• Title/Summary/Keyword: Human ART center

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Integrity of human sperm DNA assessed by the neutral comet assay and its relationship to semen parameters and clinical outcomes for the IVF-ET program

  • Chi, Hee-Jun;Chung, Da-Yeon;Choi, Soon-Young;Kim, Jong-Hyun;Kim, Gi-Young;Lee, Jae-Seok;Lee, Hee-Sun;Kim, Myung-Hee;Roh, Sung-Il
    • Clinical and Experimental Reproductive Medicine
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    • v.38 no.1
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    • pp.10-17
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    • 2011
  • Objective: To explore potential relationships between sperm DNA integrity and both semen parameters and clinical outcomes. Methods: Semen analysis of 498 samples was performed according to the 2010 criteria of the World Health Organization. The sperm DNA fragmentation Index (DFI) of the semen samples was assessed using a neutral comet assay. Results: Sperm DFI showed a significant correlation with semen parameters, including the patient's age, sperm viability, motility, morphology, and number of leukocytes (p<0.05). The sperm DFI values for asthenozoospermic (15.2%), oligoteratozoospermic (18.3%), asthenoteratozoospermic (17.5%), and oligoasthenoteratozoospermic semen samples (21.3%) were significantly higher than that observed in normozoospermic semen samples (10.5%, p<0.05). A sperm DFI value of 14% was used as a threshold of sperm DFI in assessing whether DNA was highly damaged. In 114 IVF-ET cycles, the fertilization rate of the sperm DFI <14% group (70 cycles, 61.7%) was significantly higher than that observed for the ${\geq}14%$ group (44 cycles, 55.3%), but there was no difference in the other clinical outcomes between the two groups. In the ${\geq}14%$ group, the pregnancy rates of the ICSI cycles (40.0%) and half-ICSI (44.0%) were higher than conventional IVF cycles (30.7%), but the difference was not statistically significant. Conclusion: Along with the conventional semen analysis, the sperm DFI assessed using the comet assay was shown to improve the quality of the semen evaluation. To evaluate the precise effect of ICSI on pregnancy rates in the patients who demonstrate high sperm DFI values, further study is necessary.

Integral Imaging and Digital Holography Techniques for Three-dimensional Sensing, Imaging and Display (Invited Paper) (3차원 입체영상 센싱, 이미징 및 디스플레이를 위한 집적영상 및 디지털 홀로그래피 기술)

  • Kim, Seung-Cheol;Shin, Dong-Hak;Kim, Eun-Soo
    • Korean Journal of Optics and Photonics
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    • v.25 no.4
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    • pp.169-192
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    • 2014
  • In this paper, state-of-the-art digital holography and integral imaging have been introduced as practical three-dimensional imaging and display technology. Operational principles and recent research and development activities of these technologies have been discussed, as well as a vision of their future.

The modern Chinese literature and Freudian psychoanalysis - Focuing acceptance of the Freud's theory psychoanalysis on Lu xun and Guo Mo ruo (중국 신문학 초기의 프로이드 정신분석학 수용 - 루쉰(魯迅)과 궈모뤄(郭沫若)의 프로이드 정신분석학 이론 수용을 중심으로)

  • Ko, hae-kyung
    • Cross-Cultural Studies
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    • v.37
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    • pp.81-107
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    • 2014
  • Freud's' discovery of the unconscious' is a great revolution. Because of this, human beings were able to get the opportunity to look into their inner than honest. Lu Xun and Guo Mo Ruo a pair of realism and romanticism literature representative of Chinese modern literature writers. They then absorb the psychoanalytic theory o Freud along with other Western zeitgeist, introduce them widely inliterary theory and creative practice, which was again. Lu Xun was reflected in the human world of the unconscious Freud uncovered on the basis of strict realism literary spirit, Guo Mo Ruo is in accordance with the romantic literary time for the purpose of 'art for art' depicting a man's inner psychological well did. Although Freud spirit they claim to social and literary artistry of literature based on different yarns in the Acceptance of analytical theory, has the characteristics of a common sentiment analysis method to express and describe the unconscious human potential. 5.4 When Lu Xun and Guo Mo Ruo China Journalism writers, including Freud's psychoanalytic theory has embraced the Enlightenment and the old feudal society was a major contribution to want to read exactly what the human inner hearing. Chinese modern literature writers have to accept sometimes positive, sometimes accepting the psychoanalytic theory was intellectually sharp criticism, which could be a great instrument in time to the 5.4 Enlightenment and psychological fiction novel further development.

A Study on Nail Art Service by College Woman's Life Style (여대생의 라이프스타일과 네일 아트 서비스에 관한 연구)

  • Park, Ju-Hyun;Park, Ok-Lyun;Jung, Yun-Ji
    • Korean Journal of Human Ecology
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    • v.16 no.4
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    • pp.877-884
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    • 2007
  • Today the number of economically independent women are increasing in Korea, they have a tendency to pay more attention to their appearance in their social life. Female university students show a high tendency of that kind, especially when they prepare for their job interviews. They purchase beauty products, visit beauty salons and skin care centers to improve their looks. Above all, their interest in nail art is amazingly increasing for maintaining their self-image. This study thus aims at surveying the relationship between college woman's life style and their interest in nail care, by analysing life styles of college women pursuing up-to-date style. The results show that an 'egoistic' type has a high interest in and a high frequency of using a nail care center, and 'egoistic' type's demand for the nail service is equal to or quite higher than 'appearance-oriented' type's. That means that the 'egoistic' type seems to pay much attention to how they look, because they think their neat appearance help to accomplish their ego.

Group Art Therapy Program of Low-income Broken; Effects, on Depression, Anxiety and Impulsivity (집단미술치료가 결손가정아동의 정서에 미치는 영향 -우울, 불안, 충동성 중심으로-)

  • Kim, Kui-Bok
    • Korean Journal of Human Ecology
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    • v.17 no.6
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    • pp.1237-1248
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    • 2008
  • This is to study the effects of the program of group art therapeuties for the low-income bracket children's melancholy and uneasiness. The experimental group is 19 children at their option of all 38 children in P child center at J city, Jeonbuk. The program has been going on after-school hours program at the facilities at 16times from May. 4 to August. 17, 2007. I have made the before-medium-after examination the COD, RCMAS and K-MFF through Repeated Measures ANOVA. The result is as follows, The melancholy of experimental group in comparison with that of control group is reduced by control group 0.42 and experimental group 2.67. And in lower rank group, the melancholy of experimental group for control group is dropped in the same way. In uneasiness, by the decrease of control group 0.10, experimental group 0.33, the uneasiness of experimental group for that of control group is decreased. In all drive, the drive of experimental group for that of control group is decreased by control group 0.12 and experimental group 0.33. And similarly, in all lower field, the drive of experimental group is reduced for that of control group. It is through our help to learn the proper way that they express their experience before the group members, listen to another person's story with attention, recognize their own and other's emotion, and are aware of feeling and action.

Behind and Beyond the Archaeology of the Silk Road: Laboratory Analyses in Eurasia, Some Results, Discussions, and Interpretations for Protohistory and Antiquity

  • Henri-Paul FRANCFORT
    • Acta Via Serica
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    • v.8 no.2
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    • pp.53-78
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    • 2023
  • The paper presents some new results illustrating some developments related to the concept of the Silk Road and subsequent methodological reflections. New laboratory results of scientific analyses of plants, minerals, and human remains in combination with more conventional methods of research contribute to a better understanding of the multidirectionality of exchanges in Pre- and Protohistory. Unsuspected long-distance transfers of items, especially of metals (tin) and biological materials (plants, pathogens, etc.) are discovered. Adding ancient DNA and petroglyphs to the vexed question of the Indo-European migrations across Eurasia complexifies the familiar linguistic, historical, and archaeological research landscape. Recent excavations show the impact of the adoption of artistic elements adapted from the Achaemenid arts, far in the steppe world, and up to China. Multidirectional (including North-South lanes) and multidisciplinary approaches leave space and hope for more rigorous scientific modelizations for the archaeology of Eurasia and the Silk Road.

Efficacy of ISO 9001 : 2000 Quality Management System in Human Assisted Reproductive Technology Center (보조생식술 센터에서 ISO 9001 : 2000 품질경영시스템의 도입 및 시행의 효용성)

  • Jun, Jin-Hyun;Park, Yong-Seog;Lee, Hyoung-Song;Kim, Soon-Duk;Hwang, Son-Hee;Han, Su-Kyoung;Kim, Jae-Ho;Song, In-Ok;Kang, Inn-Soo;Koong, Mi-Kyoung
    • Clinical and Experimental Reproductive Medicine
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    • v.34 no.2
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    • pp.107-115
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    • 2007
  • Objective: A quality management system of international standardization organization (ISO) 9001:2000 has been proposed to properly evaluate and improve the quality of productions and services. The purpose of this article was to describe on successful introduction and application of the ISO system to human assisted reproductive technology (ART) center. Methods: Center for reproductive medicine and infertility of Cheil General Hospital started the action for ISO 9001:2000 certification at January-2004. The ISO system of our center was monitored by measuring the customer satisfaction index and periodical internal and external audit. Reports of non-conformity described corrective and preventive actions for problems and occurrences that were not consistent with the standard process and operation of our center. Continuous management and improvement were performed on the project of customer dissatisfaction. Results: Our center has got the certification of ISO 9001:2000 for "Research and development of IVF-ET program for infertility treatment" from Korean Foundation for Quality at June-2004. The policy of ISO was "To establish the best center of reproductive medicine and infertility" and "To establish the best quality management system". We found 140 cases and 7 cases of non-conformity in internal and external audit for three years, respectively, and performed corrective action. Conclusion: Improvement of customer satisfaction, systemic documentation and transparency and efficacy of working procedure were achieved by application and operation of the ISO system to our center. This ISO system could be used as a basic institutional management system for the national guideline to the human ART center.

Studies on the Suitability and Efficiency of Human Follicular Fluid as Protein Supplement in Assisted Reproductive Technology(ART);I. Effect of Human Follicular Fluid on Meiotic Maturation of Mouse Follicular Oocytes In Vitro (생식보조시술시 단백질원으로서 인간난포액의 적합성 및 효율성에 관한 연구;I. 인간난포액이 생쥐난포란의 체외성숙에 미치는 효과)

  • Chi, H.J.;Kim, D.H.;Kim, J.Y.;Koo, J.J.;Chang, S.S.;Chung, K.S.
    • Clinical and Experimental Reproductive Medicine
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    • v.23 no.1
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    • pp.87-94
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    • 1996
  • For evaluating the suitability of human follicular fluid(HFF) as protein supplement in ART, this preliminary study was performed to examine the maturation promoting activity of HFF on mouse follicular oocytes in vitro. Mouse follicular oocytes were collected from ovaries of 21-28 day old ICR mice by puncturing the antral follicles with fine needle at 48 hours after PMSG injection. The oocytes were rinsed and cultured in modified Whittingham's $T_6$ medium containing purines or dbcAMP to maintain meiotic arrest, and different concentrations of HFF were added into the culture medium to examine the effect of HFF on releasing the oocytes from the suppressive influence of the meiotic inhibitors. As a control for HFF, the maturation promoting activity of human fetal cord serum(HFCS) was investigated and compared with the activity of HFF. While HFF was separated, by molecular weight(M.W), into high M.W. fraction(M.W>30,000) and low M.W. fraction(M.W<30,000) and the effects of the fractions on meiotic resumption were investigated in the presence of the meiotic inhibitors. Also hormone analysis was performed to compare the content of hormones in HFF with that in HFCS. Same concentrations of HFF and HFCS induced similar germinal vesicle break down(GVBD) rates of the oocytes meiotic arrested by purines(4mM hypoxanthine+0.75mM adenosine), but the extrusion rate of 1st polar body(PB) of the oocytes cultured in HFF(65.0%, P<0.05) was significantly higher than that(51.6%) in HFCS. While, in the presence of 200 M dbcAMP, the maturation promoting activity of HFF (GVBD: 70.5%, $p<10^{-6}$; 1st PB extrusion: 67.1%, $p<10^{-3}$) was significantly greater than that of HFCS(GVBD: 35.2%; 1st PB extrusion: 41.1%). The oocytes cultured in the fraction of HFF containing high M.W. components showed higher meiotic maturation rates than the oocytes cultured in the low M.W. fraction of HFF. Gonadotropins and $E_2$ were known to improve the completion of maturation changes, and the levels of these hormones were higher in HFF than in HFCS. Therefore, HFF was more effective than HFCS to use for promoting meiotic resumption of mouse oocytes in vitro.

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A Case of Pregnancy and Delivery by Round Spermatid Injection into Oocytes in Nonobstructive Azoospermia Patient (비폐쇄성 무정자증 환자에서 난자내 원형정세포 주입에 의한 임신 및 분만 1례)

  • Cho, J.H.;Shim, H.N.;Seo, J.T.;Lee, D.R.;Yoon, H.S.;Paik, H.R.;Roh, S.I.
    • Clinical and Experimental Reproductive Medicine
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    • v.23 no.3
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    • pp.327-331
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    • 1996
  • Normal fertilization and pregnancy by round spermatid was achieved from nonobstructive azoospermia patient to be believed untreatable. Therefore, it is suggested that application of round spermatid in human ART program seems to be new treatment of male infertility. Also, it will be needed further research for evaluated fertilization mechanism by round spermatid injection.

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Hierarchical Graph Based Segmentation and Consensus based Human Tracking Technique

  • Ramachandra, Sunitha Madasi;Jayanna, Haradagere Siddaramaiah;Ramegowda, Ramegowda
    • Journal of Information Processing Systems
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    • v.15 no.1
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    • pp.67-90
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    • 2019
  • Accurate detection, tracking and analysis of human movement using robots and other visual surveillance systems is still a challenge. Efforts are on to make the system robust against constraints such as variation in shape, size, pose and occlusion. Traditional methods of detection used the sliding window approach which involved scanning of various sizes of windows across an image. This paper concentrates on employing a state-of-the-art, hierarchical graph based method for segmentation. It has two stages: part level segmentation for color-consistent segments and object level segmentation for category-consistent regions. The tracking phase is achieved by employing SIFT keypoint descriptor based technique in a combined matching and tracking scheme with validation phase. Localization of human region in each frame is performed by keypoints by casting votes for the center of the human detected region. As it is difficult to avoid incorrect keypoints, a consensus-based framework is used to detect voting behavior. The designed methodology is tested on the video sequences having 3 to 4 persons.