• Journal of Microbiology and Biotechnology
• /
• v.20 no.5
• /
• pp.893-903
• /
• 2010

A cellulolytic and xylanolytic enzyme complex-producing alkalothermoanaerobacterium strain, Tepidimicrobium xylanilyticum BT14, is described. The cell was Grampositive, rod-shaped, and endospore-forming. Based on 16S rRNA gene analysis and various lines of biochemical and physiological properties, the strain BT14 is a new member of the genus Tepidimicrobium. The strain BT14 cells had the ability to bind to Avicel, xylan, and corn hull. The pH and temperature optima for growth were 9.0 and $60^{\circ}C$, respectively. The strain BT14 was able to use a variety of carbon sources. When the bacterium was grown on corn hulls under an anaerobic condition, a cellulolytic and xylanolytic enzyme complex was produced. Crude enzyme containing cellulase and xylanase of the strain BT14 was active in broad ranges of pH and temperature. The optimum conditions for cellulase and xylanase activities were pH 8.0 and 9.0 at $60^{\circ}C$, respectively. The crude enzyme had the ability to bind to Avicel and xylan. The analysis of native-PAGE and native-zymograms indicated the cellulosebinding protein showing both cellulase and xylanase activities, whereas SDS-PAGE zymograms showed 4 bands of cellulases and 5 bands of xylanases. Evidence of a cohesinlike amino acid sequence seemed to indicate that the protein complex shared a direct relationship with the cellulosome of Clostridium thermocellum. The crude enzyme from the strain BT14 showed effective degradation of plant biomass. When grown on corn hulls at pH 9.0 and $60^{\circ}C$ under anaerobic conditions, the strain BT14 produced ethanol and acetate as the main fermentation products.

• Bulletin of the Society of Naval Architects of Korea
• /
• v.8 no.1
• /
• pp.67-94
• /
• 1971

Korean fisheries industry holds 57,255 boats amounting to 269,079 tons in gross as of the end of 1967. The boats of the size under 30G.T., about sixty-two per cent of the total in tonnage, are utilized in coastal and their contribution to the total production of Korean fisheries industry is estimated at over seventy-five percent. Hull forms and construction method of them are partly in transition stage to the western tradition from the oriental tradition, which employs the chine-type straight-framed section, and tick and wide wooden planking fastened on naturally bent timber frames. And only about a half of them are mechanized. About fifty-seven per cent, 7,525 boats amounting to 91,751 gross tons, of the coastal and inshore fishing boats are those of the size ranging 5G.T. to 30G.T., which are engaged mostly in draft-and gill-netting, angling, longlining, anchovy seining, squid fishing and set-netting. The important fishing boats forming main structure of the inshore fishing fleets can be classified as 5G.T.-class multi-purpose boats, 10G.T.-class angling/longlining boats and 20G.T.-class drift-/gill-netters mostly utilized in the east-sea, 15G.T.-class anchovy seiner in the south-sea-sea, 20G.T.- and 28G.T.-class stow-netters in the west sea. Each of the three sea regions, east, south, and west, of Korean water has distinctly different characters from one another in topography, geology and sea aspects, and consequently in marine resources, fishing grounds and fishing tactics desired. Hence, the finishing boats in each sea region have also their own characters in hull form, structural features, deck design and equipments. In this report, analyzing the characteristics of the existing inshore fishing boats ranging 5G.T. to 30G.T. in size from view points of naval architecture and engineering, the author made an integrated investigation of their characteristics, covering size and principal dimensions, hull form, deck design, structural features and mechanization, in close connection with the natural circumstances of Korean water and local techno-socio-economic problems, and, finally, made some suggestions for the rational improvement or modernization of the inshore fishing boats. Further details of the characteristics of Korean inshore finishing boats are referred to the drawings of them compiled by the author and given in the reference[23] listed at the end of this report.

• Journal of the Society of Naval Architects of Korea
• /
• v.55 no.2
• /
• pp.136-143
• /
• 2018

3-D templates are produced to evaluate completeness of the shell plates during the forming process, which is an essential step for the ship production. They are mostly produced in advance during the detail/production design stage, but occasionally they are requested by the shell plate forming department, because it is impossible to predict accurately the necessities of them at the design stage. This results in a huge loss of man-hour and a bottleneck. In order to resolve this issue while reducing the dependence on other department, the process of manufacturing the 3-D templates needs to be automated. Therefore, this study proposes an automatic system that calculates the manufacturing information of the 3-D templates with only geometric information of the shell plates. The system considers the thickness and the cutting method of the parts of the 3-D templates and some options are provided to reflect the intention of the worker.

• Journal of the Society of Naval Architects of Korea
• /
• v.36 no.3
• /
• pp.122-133
• /
• 1999

Overlap, excessive wide gaps, and errors between blocks during erection process increases cost and man-hour. In this paper, a system to simulate the assembly process is suggested and a plane block assembly of welding deformation with gravity is simulated in consideration of assembly order, deformations and errors occurring in the cutting and forming processes are not considered and welding deformations are acquired by equivalent stiffness and load method from experiments and hull double bottom plane block is assembled on a assembly order by panel method. It is certified that according to the order of assembly, intermediate product shape affects rigidity which affects welding deformations. Assembly order must be considered in the assembly process. It is certified that the gravity has important role in the assembly process.

• International Journal of Ocean System Engineering
• /
• v.1 no.1
• /
• pp.46-51
• /
• 2011

In a line heating process for hull forming, the phase of the steel transforms from austenite to martensite, bainite, ferrite, or pearlite depending on the actual speed of cooling following line heating. In order to simulate the water cooling process widely used in shipyards, a heat transfer analysis on the effects of impinging water jet, film boiling, and radiation was performed. From the above simulation it was possible to obtain the actual speed of cooling and volume percentage of each phase in the inherent strain region of a line heated steel plate. Based on the material properties calculated from the volume percentage of each phase, it should be possible to predict the plate deformations due to line heating with better precision. Compared to the line heating experimental results, the simulated water cooling process method was verified to improve the predictability of the plate deformation due to line heating.

• Journal of the Society of Naval Architects of Korea
• /
• v.33 no.4
• /
• pp.142-149
• /
• 1996

Pyramid type three roll bending machines are widely used in roll-bending process to produce singly curved plate. In forming singly curved plate, controlling the vertical displacement of the center roller is most important to acquire the shape required and automation system of the process. In this paper roller bending process is modeled as an elastic-plastic phenomenon and analyzed using beam theory and finite element method. In finite element analysis the workpiece is modeled by using beam elements and plane strain elements respectively. Through the analyses vertical center roller displacement is obtained to get constant curvature distribution along arc length. The relationship between center roller displacement and curvature in steady state as well as residual stress and strain along plate thickness direction are calculated through finite element analysis.