• 한국식품영양과학회지
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• 제34권8호
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• pp.1284-1288
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• 2005

멜라닌 색소 생성에 관여하는 tyrosinase 유전자의 발현을 억제하는 물질을 탐색하고자 tyrosinase 프로모터를 지닌 Bl6 mouse melanoma cell에 어성초 메탄올 추출물을 처리한 결과 메탄올 추출물은 10$\mu$g/mL와 100$\mu$g/mL의 농도에서 대조군에 비해서 약 13$\%$와 45$\%$의 억제효과를 나타냈으며, 세포생존율은 1$\mu$g/mL, 10$\mu$g/mL, 100$\mu$g/mL의 농도에서 약 104$\%$, 101$\%$, 74$\%$로서 세포독성이 낮게 나타났다. Butyl alcohol과 물 분획물은 tyrosinase 유전자의 발현을 억제하는 효과가 없 었지만 methylene chloride 분획물은 tyrosinase 유전자의 발현을 억제하는 효과를 나타내었으며, 특히 methylene chloride 분획물은 10$\mu$g/mL와 100$\mu$g/mL의 농도에서 각각 약 82$\%$와 48$\%$의 발현율을 나타냄으로써 대조군에 비해 tyrosinase유전자의 발현을 크게 억제하였다.

• 동의생리병리학회지
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• 제20권3호
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• pp.690-696
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• 2006

Houttuynia cordata Thunb, well known as 'E-Sung-Cho' in Korea, is a traditional medicinal plant generally used in Oriental medicine therapy. In the present study, we investigated the effect of water extract of H. cordafa (WEHC) on the growth of human breast carcinoma MCF-7 cells. Exposure of MCF-7 cells to WEHC resulted in growth inhibition and apoptosis in a dose-dependent manner as measured by MTT assay and fluorescent microscope. The anti-proliferative effect of WEHC was associated with a dose-dependent up-regulation of cyclin-dependent kinase inhibitor p21 in a p53-independent fashion. We found WEHC decreased the levels of cyclooxygenase (COX)-2 and inducible nitric oxide synthease (iNOS) expression without significant changes in the expression of COX-1 , which was correlated with a decrease in prostaglandin $E_2{\;}(PGE_2)$ synthesis. Taken together, these findings provide important new insights into the possible molecular mechanisms of the anti-cancer activity of H. cordata.