• 제목/요약/키워드: House Cultivation

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Detection of Waterborne Pathogens in Public Bath Houses by PCR-Reverse Blot Hybridization Assay (PCR-REBA) (분자생물학적 방법인 PCR-REBA를 이용한 대중목욕탕 수질 중 수인성병원성미생물 검출)

  • Song, Woon-Heung;Choi, Seung-Gu;Yang, Byoung-Seon;Lee, Jae-Sang
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.12 no.8
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    • pp.3517-3522
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    • 2011
  • Contamination of public bath water by waterborne pathogens can cause disease outbreaks and contribute to background rates of disease. The aim of this study is to determine the prevalence of waterborne pathogens in public baths. A total of 30 water samples were collected from 30 different public baths in seoul, Korea. Pathogens in water samples were concentrated by 0.45 ${\mu}m$ nitrocellulose membrane filter, analyzed by both cultivation and polymerase chain reaction-reverse blot hybridization (PCR-REBA) of partial 16S rRNA gene. Various microorganisms including Escherichia coli and Shigella spp. were identified by microbiological cultivation. E. coli, Shigella spp., Salmonella spp., Pseudomonas spp. and Mycobacterium spp. were identified by PCR-REBA. Our results suggest that appropriate hygiene practice and continuous monitoring is needed for reducing health risk associated with public bath houses.

Analyzing the Applicability of Greenhouse Detection Using Image Classification (영상분류에 의한 하우스재배지 탐지 활용성 분석)

  • Sung, Jeung Su;Lee, Sung Soon;Baek, Seung Hee
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.30 no.4
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    • pp.397-404
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    • 2012
  • Jeju where concentrates on agriculture and tourism, conversion of outdoor culture into cultivation under structure happens actively for the purpose of increasing profit so continuous examination on house cultivation area is very important for this region. This paper is to suggest the effective image classification method using high resolution satellite image to detect the greenhouse. We carried out classification of greenhouse using the supervised classification and rule-based classification method about Formosat-2 images. Connecting result of two classification try to find accuracy improvement for greenhouse detection. Results about each classification method were calculated the accuracy by comparing with the result of visual detection. As a result, mahalanobis distance among the supervised methods was resulted in the highest detection. Also, it could be checked that detection accuracy was improved by tying with result of supervised method and result of rule-based classification. Therefore, it was expected that effective detection of greenhouse would be feasible if henceforward further study is performed in the process of connecting supervised classification and rule-based classification.

The Effect of Segmented tuber Size on Sprouting and Yield of Yam (Dioscorea opposota Thunb.) (소절편 크기가 둥근마 출아 및 수량에 미치는 영향)

  • Chang, Kwang-Jin;Kim, Ki-Sun;Park, Byoung-Jae;Park, Ju-Hyun;Park, Cheol-Ho
    • Korean Journal of Plant Resources
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    • v.20 no.2
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    • pp.99-103
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    • 2007
  • Tuber yield and content of general component and diosgenin which is a main bioactive property were investigated in order to determine the growth characteristics of round typed yam (Dungeon-ma, Dioscorea opposita L.) and the potential of artificial culture at Suwon, Korea. Sprouting rates of segmented small tubers such as 3, 5, 7, 9, and 12g were respectively 60.3, 80.5, 85.3, 98.6 and 99.3% when they were investigated 30 days after planting. Segmented small tubers of 3, 5, 7, 9, and 12g enlarged respectively to 66, 87, 98, 120, and 140g in the green-house cultivation. Segmented small tubers of 3, 7, 12, 18, and 23g enlarged respectively to 35.2, 124.7, 142.7, 174.8, and 200,7g in the open-air field cultivation.

Cultivating Status of Paeonia lactiflora Pallas in Central Part of Gyeong Bug Province (경북 중부지역에서의 작약 재배실태)

  • Kim, Se-Jong;Park, So-Deuk;Whang, Wheong-Baeg;Kim, Jae-Cheol
    • Korean Journal of Medicinal Crop Science
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    • v.3 no.3
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    • pp.259-264
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    • 1995
  • This study was carried out to find cultivation condition of peony in Euiseong district from 1994 to 1995. 1. Age distribution in cultivation of peony was from 30's to 50's and cultivated years was mainly from 5 years to 10 years, but there was more than 15 years 2. Cultivating area per house hold were 1300 pyeong average, there were moderate $400{\sim]1000$ pyeong and also those are occupied 22.6% more than 2000 pyeong. 3. The mothods of transplanting in Euiseong peony which Euiseong district was mostly divided peony but Yeongchen district used seedling stock. No. of sprout was mostly $3{\sim}4$ and planting time was mainly from Mid to Late of October. 4. The percentage of sterilization of soil and seedling stock was 77. 4%, in mulching by vinyl in tran­splanting 64. 5% in black vinyl and 25. 8% in white one. 5. Number of plant in peony planting was $3000{\sim}4000$ plant per l0a even more there was more than 8000 plant. 6. Chemical spraying time in peony field was 2 time in $1{\sim}2$ years cultivating, and $3{\sim}4$ time in $3{\sim}4$ years one. 7. Condition of fertilizer application in peony field was 40% in non-application, but $56.7{\sim}76.7%$ in 2 year to 3 years, and the time of fertilizer was mainly 3 time. 8. Drying time peony after havesting was from 12 to 24 hours by briquet stove and 24 hours by ma­chine of hot wind, also dry method of peony was mixed briquet stove and hot wind machine.

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A research on productivity of Cortinellus edodes (Berk) Ido et Imai cultivated on timber bed (골목일대(榾木一代)의 표고버섯 발생량(發生量)에 대(對)한 연구(硏究))

  • Chung, Tae Kyo
    • Journal of Korean Society of Forest Science
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    • v.3 no.1
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    • pp.23-27
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    • 1963
  • A research was made through a experimental cultivation during a period of 7 years (1956~1962) to determine the yearly yield, the total gross yield over 7 year's period and the maximum availability of stock timbers used as the germination bed of cortinellus edodes (Berk) Ido et Imai. The results are summarized as follows : (1) Research and plot scheme: Two experimental plots were established-plot "A" in the experimental forest of this college; plot "B" in the campus woods, 400 timber beds were furnished in each. The kinds of timbers used are : Quercus Serrata Thumb 140. Quercus acutissima Carr. 140, Quercus crispula BI 120. The environmental condition in plot "A" was better then that in plot "B" and the former showed greater yield than the latter. (2) yield : In plot "A" the total yield was 58.875kg and some of the timbers was still continuing germ in this seven after closing of the plot. In plot "B" the total yield was 56.90kg and after the sixth year no more germination was observed. In conclusion, plot "A" showed greater yield and longer germination than plot "B". (3) Germination efficacy and the kinds of timber: The best result was abserved on Quercus serrata, thumb Quercur acutissima Carr, stood next and then came Quercus crispula BI. Betula platyphylla was tried, but the result was worst. (4) Availability limits of timber beds: Quercus serrata Thumb with diameter 12~15cm had the longest availability more than (6~7 years) and Quercus acutissima Carr with diameter 12~15cm showed 6~7 year's availability. The shortest availability was found in Quercus crispula with diameter 6~9cm. Any kinds of timber with diameter 6~9cm had only 4~5 years of availability (5) In the table showing the yearly germination quantity, the figure of December and Jaunary of the 4th and 5th year shows the yield obtained in the semi-cellar paper house. This tells that there is good promise of winter cultivation of cortinellus edodes (Berk) Ido et Imai on matured timber beds.

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Comparison of Growth and Constituent of Ostericum praeteritum Kitag. by Seedling Cultivation Methods (강활 육묘 재배양식에 따른 생육 및 성분 비교)

  • Park, Jun-Hong;Kim, Sung-Han;Shon, Hyeong-Rak;Jang, Won-Cheol;Kim, Jae-Cheol
    • Korean Journal of Plant Resources
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    • v.25 no.5
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    • pp.586-590
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    • 2012
  • This study was conducted to investigate the seedling cultivation methods of Ostericum praeteritum Kitag. The results are as follows ; Ostericum praeteritum transplanted from wild seedlings and seedling cultured in the open field grew quite well, but their bolting rate was higher than other seedlings resulting in low root production. Seedling cultured in tray pot of green house produced good quality root as 426 kg/10a indicating the most adequate seedling method for Ostericum praeteritum production. Main compounds of Ostericum praeteritum roots were found as oxypeucedanin and bisabolangelone, and contents of wild seedlings were lower than theirs of the other seedlings methods.

Energy Consumption in Mushroom Canning Factory (양송이통조림 가공중의 에너지소비량 조사연구)

  • Lee, Dong-Sun;Park, Know-Hyun;Shin, Hyu-Nyun;Shin, Dong-Hwa
    • Korean Journal of Food Science and Technology
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    • v.13 no.4
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    • pp.328-333
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    • 1981
  • As a step to investigate energy conservation in canneries, energy consumption pattern and energy usages of various unit operations in a mushroom cannery were examined. The results are as follows; 1. In the mushroom cannery, fuel oil and electricity were used mainly for temperature control of mushroom growing house in winter and various cultivation operation respectively. To grow and process 1 kg of mushroom, thermal energy of 4634 kcal and electrical energy of 0. 116 kwh were consumed. About 80% of all energy was consumed for cultivation. 2. Steam qualities at each respective processing line were $92{\sim}94%$, giving no great differences among lines. 3. As a direct energy in 1 day processing operations of 8 tons of mushroom, thermal energy of $301.5{\times}10^{4}kcal$ and electrical energy of 60.1 kwh were used. The energy intensive operations were blanching (35%) and retorting (38%).

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Artificial cultivation of Medicinal Mushroom, Phellinus linteus using Mulberry logs

  • Hong, In-Pyo;Sung, Gyoo-Byung;Chung, In-Mo;Lee, Won-Chu
    • Journal of Sericultural and Entomological Science
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    • v.44 no.2
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    • pp.74-81
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    • 2002
  • The optimal conditions for mycelial growth of P. linteus ASI 26011 were 25-30$^{\circ}C$ and pH 6.0, respectively. The mycelial growth of P. linteus was excellent on MCM medium. In case of carbon sources, the mycelial growth of P. linteus was best on the culture media that were contained with sucrose, mannose and glucose. Potassium nitrate and sodium nitrate were good for the mycelial growth of P. linteus as a nitrogen source. For comparison of the mycelial colonization of P. linteus on logs, several techniques of inoculation were tested; the sterilized short log inoculation, drilling inoculation and log-end sandwich inoculation. The mycelial colonization of P. linteus on logs was good in the treatment of sterilized short log inoculation, but poor in the traditional methods such as drilling inoculation and log-end sandwich. The initial mycelial growth and the full mycelial colonization of P. linteus were the best on 20 cm logs under the condition of 42% of moisture content in log. Also the initial mycelial growth of P. linteus was accelerated over 12 hours of sterilization. Burying method of logs after 5-6 months of incubation was the best for formation of basidiocarp of P. linteus. The formation of fruiting body of P. linteus was quite good in the cultivation house at the 31-35$^{\circ}C$ and over 96% of relative humidity.

Exploring the quality standard of Phellinus spp through β-glucan content and sensory evaluation (β-glucan 함량과 관능검사를 통한 상황버섯 품질기준 모색)

  • Shin, Yong-Wook
    • The Korea Journal of Herbology
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    • v.32 no.4
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    • pp.47-52
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    • 2017
  • Objective : Phellinus spp. mushroom is an object of interest because it has excellent anticancer effect. Owing to the similalarities in the morphology, Phellinus linteus and Phellinus baumii are often used as same Sang Hwang Mushroom in the Korean market.. The quality control for mushrooms is needed because there are many differences in the efficacy according to cultivation method and cultivation area. Therefore, a reliable authentication method of these herbal medicine is necessary to compare and measure the amount of beta-glucan which is known to have a hypoglycemic effect, from the mushrooms collected in various regions Methods : 7 samples of medicinal mushrooms supplying phellinus spp. were collected in Korea, China and Cambodia. We investigated the hardness, colors, extract ratio, ${\alpha}-amylase$ and ${\alpha}-glucosidase$ inhibitory activities, glucose transporter 4 (GLUT-4) expressions of water extracts from Phellinus spp and also MTT assay were examined for cell toxicity. Results : The results revealed that Phellinus spp.water Ext.inhibited ${\alpha}-glucosidase$ activity. glucose transporter 4 (GLUT-4), the key insulin signaling pathway transcription factor, was remarkably increased by the Phellinus baumii water extract Conclusions : These results suggest that The more yellowish the mushroom is, the lower the hardness, the more the content of ${\beta}-glucan$ is proportional. Because the more ${\beta}-glucan$, the greater the effect of hypoglycemia. compared to the hypoglycemic effect, Phellinus Baumii grown at hanging on selves for 7 month in the green house is the best.

Seed Characteristics of Parasenecio firmus Kom. and Its Growth Comparison among Forest Stands (병풍쌈의 종자 및 임분별 생장 특성)

  • Park, Wan-Geun;Kim, Young-Sol;Lee, Hak-Bong;Kim, You-Sin;Kim, Nam-Jun;Kim, Nam-Young
    • Journal of Forest and Environmental Science
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    • v.26 no.3
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    • pp.219-223
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    • 2010
  • It has long been recognized that wild vegetation are sort of improving fitness. Many peoples are now ingesting them instead of the vegetables that are grown in green house. In this regard, Parasenecio firmus, which is considered to be one of the rare species in Korea, has been given a lot of attentions as edible green plants recently. Hence it may be necessary to provide the information people who want to commercially cultivate the plants. Regarding appropriate forest stands that are fit for the cultivation. Firstly, we collected the seeds from 4 natural habitats to find out how many seeds are fully matured in the natural conditions. The quality of the seeds collected were found to be very low. However, it appeared as clear differences when screened seeds were grown in covered seed beds over winter. In this case the germination rate of the seeds was 95%, indicating that there may be no difficulty for mass production of plantlets. Secondly, in the field of planting test in an appropriate cultivating site among 6 of forest stands, those in Pinus koraiensis stand were the highest in survival rate, but somewhat low with those in Quercus mongolica and broad-leaved stands. Thus, it was demonstrated that forest stands dominated with conifer like P. koraiensis are more appropriate for the cultivation of P. firums.