• Title/Summary/Keyword: Hot-water extract

Search Result 995, Processing Time 0.029 seconds

The Effects of ChenwhangBosindan(CBD) Hot Water Extract & Ultra-fine Powder on the Alzheimer's Disease Model (천왕보심단(天王補心丹) 열수추출물(熱水抽出物), 초미세분말제형(超微細粉末劑形)이 Alzheimer's Disease 병태(病態) 모델에 미치는 영향(影響))

  • Choi, Kang-Wook;Jung, In-Chul
    • Journal of Oriental Neuropsychiatry
    • /
    • v.19 no.2
    • /
    • pp.77-93
    • /
    • 2008
  • Objective : This experiment was designed to investigate the effect of the CBD hot water extract & ultra-fine Powder on Alzheimer's Disease Model Induced by ${\beta}A$. Method : The effects of the CBD hot water extract on expression of interleukin-1 beta($IL-1{\beta}$), $TNF-{\alpha}$ mRNA and production of IL-6, $TNF-{\alpha}$ in BV2 microglial cell line treated by lipopolysacchaide(LPS). The effects of the CBD hot water extract & ultra-fine powder on (1) the behavior (2) expression of $IL-1{\beta}$, tumor necrosis factor-alpha($TNF-{\alpha}$), (3) the infarction area of the hippocampus in Alzheimer's diseased mice induced with ${\beta}A$ were investigated. Result : The CBD hot water extract suppressed the expression of $IL-1{\beta}$, $TNF-{\alpha}$ mRNA in BV2 microglia cell line treated with LPS. The CBD hot water extract significantly suppressed the production of $IL-1{\beta}$, $TNF-{\alpha}$ in BV2 microglial cell line treated with LPS. The CBD hot water extract & ultra-fine powder a significant inhibitory effect on the memory deficit was shown for the mice with Alzheimer's disease induced by ${\beta}A$ in the Morris water maze experiment, which measured step-through latency and distance movement-through latency. The CBD hot water extract & ultra-fine powder significantly suppressed the expression of $IL-l{\beta}$ and $TNF-{\alpha}$ protein in the microglial cell of mice with Alzheimer's disease induced by ${\beta}A$. The CBD hot water extract & ultra-fine powder suppressed the over-expression of AChE activity in the serum of the mice with Alzheimer's disease induced by ${\beta}A$. The CBD hot water extract & ultra-fine powder reduced infarction area of hippocampus, in the mice with Alzheimer's disease induced by ${\beta}A$. Conclusions : These results suggest that the CBD hot water extract & ultra-fine powder may be effective for the prevention and treatment of Alzheimer's disease. Investigation into the clinical use of the CBD hot water extract & ultra-fine powder for Alzheimer's disease is suggested for future research.

  • PDF

Effects of Sungsimjihwang-tang Hot Water Extract & Ultra-fine Powder on the Alzheimer's Disease Model (성심지황탕(醒心地黃湯) 열수추출물과 초미세분말제형이 Alzheimer's Disease 병태 모델에 미치는 영향)

  • Min, Kyung-Jik;Lee, Sang-Ryong;Jung, In-Chul
    • Journal of Physiology & Pathology in Korean Medicine
    • /
    • v.22 no.5
    • /
    • pp.1178-1191
    • /
    • 2008
  • This experiment was designed to investigate the effect of the SSJHT hot water extract & ultra-fine Powder on Alzheimer's Disease Model Induced by ${\beta}A$. The effects of the SSJHT hot water extract on expression of IL-1RA, $IL-1{\beta}$$, IL-6, IL-10, $TNF-{\alpha}$, NOS-II, COX-2 mRNA and production of $IL-1{\beta}$, IL-6, $TNF-{\alpha}$ in BV2 microglial cell line treated by lipopolysacchaide(LPS). The effects of the SSJHT hot water extract & ultra-fine powder on (1) the behavior (2) expression of $IL-1{\beta}$, $TNF-{\alpha}$, MDA, CD68, CD11b and AChE (3) and the infarction area of the hippocampus in Alzheimer's diseased mice induced with ${\beta}A$ were investigated. The SSJHT hot water extract suppressed the expression of $IL-1{\beta}$, IL-6 and $TNF-{\alpha}$, NOS-II, COX-2 mRNA and increased IL-1RA, IL-10 in BV2 microglia cell line treated with LPS. The SSJHT hot water extract suppressed the production of $IL-1{\beta}$, IL-6, $TNF-{\alpha}$ significantly in BV2 microglial cell line treated with LPS. The SSJHT hot water extract & ultra-fine powder a significant inhibitory effect on the memory deficit was shown for the mice with Alzheimer's disease induced by ${\beta}A$ in the Morris water maze experiment, which measured step-through latency. The SSJHT hot water extract & ultra-fine powder suppressed the expression of $TNF-{\alpha}$$, $L-1{\beta}$ protein significantly in the microglial cell of mice with Alzheimer's disease induced by ${\beta}A$. The SSJHT hot water extract & ultra-fine powder reduced the MDA and suppressed the over-expression of CD68, CD11b in the mice with Alzheimer's disease induced by ${\beta}A$. The SSJHT hot water extract & ultra-fine powder significantly decreased AChE activity in the serum of the mice with Alzheimer's disease induced by ${\beta}A$. The SSJHT hot water extract & ultra-fine powder reduced infarction area of hippocampus. and controlled the injury of brain tissue in the mice with Alzheimer's disease induced by ${\beta}A$. The results suggest that the SSJHT hot water extract & ultra-fine powder may be effective for treatment of Alzheimer's disease. Investigation into the clinical use of the SSJHT hot water extract & ultra-fine powder for Alzheimer's disease is suggested for future research.

Comparison and Analysis of Several Main Components between Hot Water Extract and Alcohol Extract of Gamichungsangbohatang (가미청상보하탕(加味淸上補下湯) 열수추출물(熱水抽出物)과 알코올추출물(抽出物)의 지표물질(指標物質) 및 패턴의 비교분석(比較分析))

  • Min, Jung-Ki;Jung, Hee-Jae;Rhee, Hyung-Koo;Jung, Sung-Ki
    • The Journal of Internal Korean Medicine
    • /
    • v.27 no.1
    • /
    • pp.55-71
    • /
    • 2006
  • Objectives : To find out more pharmacologically efficient way of extraction of herbal dicoction, Gamichungsangbohatang (GMCSBHT). Methods : Several main components of GMCSBHT was compared and analysed between hot water extract of GMCSBHT and Alcohol(70% ethanol) extract of GMCSBHT via HPLC method. Results : Hot water extract of GMCSBHT showed relatively more component content than ethanol extract of GMCSBHT. Also weighted mean of main components of hot water extraction of GMCSBHT was higher than that of alcohol extract of GMCSBHT. But from chromatographic pattern analysis of matching ratio and similarity ratio showed that these two forms of extracts might have different chemical composition, and 3D PDA plot of alcohol extract of GMCSBHT showed high peaks near UV $190{\sim}220nm$ which was invisible in hot water extract of GMCSBHT. Conclusion : Alcohol extract of GMCSBHT may have some special components which do not exist in hot water extract of GMCSBHT.

  • PDF

Effect of Rot Water Soluble Extract from Eleutherococcus and Senticosus and Dietary Carnitine on the Lipid Metabolism and Antioxidant Defense System of Rats on Hypercholesterol Diet (가시오가피 열수추출물과 카르니틴 공급이 고콜레스테롤 식이를 공급한 흰쥐의 지질대사와 항산화계에 미치는 영향)

  • 이승교;원향례
    • The Korean Journal of Community Living Science
    • /
    • v.15 no.1
    • /
    • pp.105-113
    • /
    • 2004
  • This study investigated the synergy effects of hot water soluble extract from Eleutherococcus senticosus and dietarty carnitine on the lipid metabolism and antioxidant defense system of rats on hypercholesterol diets. Sprague-Dawley male rats were fed either a hypercholesterol diet with water or a hot water soluble extract from Eleutherococcus senticosus and dietarty carnitine. The experimental groups consisted of the control group (CO), the group supplied with hot water soluble extract from Eleutherococcus senticosus(ES), and the group supplied with hot water soluble extract from Eleutherococcus senticosus and dietarty carnitine(ESC). Eleutherococcus senticosus was extracted, made into 0.5% solution, and fed to the ES and ESC groups. A 3% carnitine diet was supplied to the ESC group. Hypercholesterol diets contained 18% beef tallow and 5% cholesterol. After 4 weeks of administering these diets, serum and liver were obtained and the level of serum lipid and the activities of GOT and GPT were measured. In addition, the level of liver lipid and TBARS and the activity of GSH-Px were measured. The results were as follows: 1) Weight gain and FER in the group supplied with hot water soluble extract from Eleutherococcus senticosus and dietarty carnitine(ESC) was low significantly(P<0.05). 2) In the groups supplied with hot water soluble extract from Eleutherococcus senticosus(ES) and the group supplied with hot water soluble extract from Eleutherocorcus senticosus and dietarty carnitine(ESC), total serum cholesterol levels were both significantly low (P<0.05,P.0.01). 3) Serum GOT activity was significantly low(P<0.05) in the group supplied with hot water soluble extract from Eleutherococcus senticosus(ES) and in the group supplied with hot water soluble extract from Eleutherococcus senticosus and dietary carnitine(ESC). There was no difference between serum GPT activity in the control group and the groups supplied with hot water soluble extract from Eleutherococcus senticosus or dietary carnitine. 4) Liver triglyceride was low (P.0.05) in the group supplied with hot water soluble extract from Eleutherococcus senticosus and dietarty carnitine(ESC). No difference was observed in other lipid composition, TBARS level, and GSH-px activity in liver between the control group and the experimental groups. In summary, the effects of hot water soluble extract from Eleutherococcus senticosus and dietarty carnitine on the lipid metabolism and antioxidant defense system (such as weight gain, FER, total serum cholesterol and liver triglyceride) were low when Eleutherococcus senticosus and dietarty carnitine were supplied simultaneously.

  • PDF

The Effects of Ondam-tanghapChongmyung-tang(Wendantanghecongmingtang) Hot Water Extract & Ultra-fine Powder on Proinflammatory Cytokine of Microglia and Memory Deficit Model (온담탕합총명탕(溫膽湯合聰明湯) 연수추출물과 초미세분말이 cytokine과 건망증 생쥐모델 기억력감퇴에 미치는 영향)

  • Jang, Young-Joo;Jung, In-Chul;Lee, Sang-Ryong
    • Journal of Oriental Neuropsychiatry
    • /
    • v.20 no.1
    • /
    • pp.59-73
    • /
    • 2009
  • Objectives : This experiment was designed to investigate the effect of the ODTCMT hot water extract & ultra-fine Powder on Alzheimer's Disease Model Induced by 13A. Methods : The effects of the ODTCMT hot water extract on expression of IL-l${\beta}$, IL-6, TNF-${\alpha}$ in BV2 microglial cell line treated by lipopolysacchaide(LPS) were investigated. The effects of the ODTCMT hot water extract & ultra-fine powder on the behavior of the memory deficit mice induced by scopolamine were investigated. Results : 1. The ODTCMT hot water extract significantly suppressed the production of TNF-${\alpha}$, IL-6 and IL-l${\beta}$ in BV2 microglial cell line treated with LPS. 2. The ODTCMT hot water extract & ultra-fine powder groups showed significantly inhibitory effect on the scopolamine-induced impairment of memory in the experiment of Morris water maze. Conclusions : These results suggest that the ODTCMT hot water extract & ultra-fine powder may be effective for the prevention and treatment of Alzheimer's disease. Investigation into the clinical use of the ODTCMT hot water extract & ultra-fine powder for Alzheimer's disease is suggested for future research.

  • PDF

The Effects of BunSimGiEumGami-Bang(Fenxinqiyinjiameifang) on Serotonin of P815 cell (분심기음가미방(分心氣飮加味方)의 항산화능과 serotonin 대사에 미치는 영향)

  • Lim, Jae-Won;Jung, In-Chul;Lee, Sang-Ryong
    • Journal of Oriental Neuropsychiatry
    • /
    • v.22 no.2
    • /
    • pp.147-162
    • /
    • 2011
  • Objectives : This experiment was designed to investigate the effect of the BSGE hot water extract on serotonin biosynthesis of depression model. Methods : The cytotoxicity of the BSGE hot water extract was analyzed by MTT assay on P815 cell. The antioxidant activity was measured by DPPH free radical-scavenging activity and SOD activity on P815 cell. The quantity of 5-HT and expression of TPH-1, AAADC and MAOa mRNA was measured by of HPLC profle Analysis on P815 cell. Results : 1. The BSGE hot water extract increased DPPH free radical-scavenging activity and SOD activity on P815 cell. 2. The BSGE hot water extract increased significantly the quantity of 5-HT. 3. The BSGE hot water extract increased the expression of TPH-1 mRNA. Conclusions : This experiment shows that the BSGE hot water extract might be effective for the prevention and treatment of depression. Investigation into the clinical use of the BSGE hot water extract for depression is suggested for future research.

Optimal Process of Eel Hot-Water Extract for Proper Edible Volume (장어고음의 적정 음용량을 위한 제조공정의 설정)

  • Cho, Young-Je;Jung, Ho-Jin;Kim, Yun-Chul;Oh, Sang-Min;Son, Myoung-Jin;Kim, Seung-Mi;Sim, Kil-Bo
    • Journal of Fisheries and Marine Sciences Education
    • /
    • v.18 no.3
    • /
    • pp.374-378
    • /
    • 2006
  • Eel is Anguilla japonica and Anguillidae family as a freshwater fish, and Japanese name is Unagi. The content of vitamin A of eel muscle are about 4,000IU/100g and they have many effective components, so it is known as healthy food. Therefore, many people have eel that cooked roast and hot-water extract in Korea. WHO encouraged that daily edible content of vitamin A is 2,000IU. Now, Japan government proposes the weight of roasted eel in lunch basket as 50g. But, Korea government does not proposes in every related aspects. Therefore, we researched content of vitamin A in hot-water extract from eel and optimal processing condition of hot-water extract from eel, in order to determine a proper edible volume. The content of vitamin A in eel hot-water extract sold at a market was about 200 IU. This study resulted from the fact that many eel hot-water extracts lacked the vitamin A. The reason was that the lipids were removed for taste on processing. But, The content of vitamin A in eel hot-water extract which didn't removed lipid on processing was about 800IU. Therefore, in drinking for 3packs per day, eel hot-water extract might have adjusted 100mL per pack which is almost consistent with the recommended dietary allowance of vitamin A in WHO.

The Effects of KakamBoyangHwanohTang(KBHT) Hot Water Extract & Ultra-fine Powder on the Alzheimer's Disease Model (가미보양환오탕(加味補陽還五湯) 열수추출물, 초미세분말제형이 Alzheimer's Disease 병태 모델에 미치는 영향)

  • Seo, Seung-Ho;Jung, In-Chul;Lee, Sang-Ryong
    • Journal of Oriental Neuropsychiatry
    • /
    • v.19 no.3
    • /
    • pp.69-84
    • /
    • 2008
  • Objective: This experiment was designed to investigate the effects of the KBHT hot water extract & ultra-fine powder on Alzheimer's Disease Model Induced by $\beta$A. Method: The effects of the KBHT hot water extract on expression of proinflammatory cytokine mRNA in BV2 microglial celll cell line treated by lipopolysacchaide(LPS). The effects of the KBHT hot water extract & ultra-fine powder on (1) the behavior (2) AChE in serum (3) the infarction area of the hippocampus, and brain tissue injury in Alzheimer's diseased mice induced with $\beta$A were investigated. Results: 1. The KBHT hot water extract suppressed the expression of proinflammatory cytokine mRNA in BV2 microglial cell line treated with LPS. 2. The KBHT hot water extract & ultra-fine powder a significant inhibitory effect on the memory deficit was shown for the mice with Alzheimer's disease induced by $\beta$A in the Morris water maze experiment, which measured stop-through latency and distance movement-through latency 3. The KBHT hot water extract & ultra-fine powder suppressed the over-expression of AChE activity in the serum of the mice with Alzheimer's disease induced by $\alpha$A. 4. The KBHT hot water extract & ultra-fine powder suppressed the expression of TNF-$\alpha$, IL-l$\beta$ protein significantly in the microglial cell of mice with Alzheimer's disease induced by 1$\beta$A. 5. The KBHT ultra-fine powder reduced infarction area of hippocampus significantly in the mice with Alzheimer's disease induced by $\beta$A. Conclusions: These results suggest that the KBHT hot water extract & ultra-fine powder may be effective for the prevention and treatment of Alzheimer's disease. Investigation into the clinical use of the PMCMT hot water extract & ultra-fine powder for Alzheimer's disease is suggested for future research.

  • PDF

The Effects of DoDamTanghapChongMungTang(Daotantanghecongmingtang) on LPS induced-Microglia and Memory Deficit Mice Model (도담탕합총명탕(導痰湯合聰明湯)이 LPS로 처리된 microglia 및 기억력 감퇴 생쥐 모델에 미치는 영향)

  • Park, Dae-Myung;Lee, Sang-Ryong;Jung, In-Chul
    • Journal of Oriental Neuropsychiatry
    • /
    • v.22 no.2
    • /
    • pp.107-128
    • /
    • 2011
  • Objectives : This experiment was designed to investigate the efficacy of DDTCMT hot water extract & ultra-fine powder on Alzheimer's Disease Model. Methods : The effects of the DDTCMT hot water extract on expression of IL-$1{\beta}$, IL-6, TNF-${\alpha}$, COX-2, NOS-II, IL-10, IL-1 receptor antagonist mRNA and production of IL-$1{\beta}$, IL-6, TNF-${\alpha}$ in BV2 microglial cell line treated by lipopolysacchaide(LPS) were investigated. Expression of NO, ROS in BV2 microglial cell line treated by LPS and AChE activity in PC-12 cell treated by NGF were investigated. anti-AChE was observed through Western blot analysis. The effects of the DDTCMT hot water extract & ultra-fine powder on the behavior of the memory deficit mice induced by scopolamine were investigated. Results : 1. The DDTCMT hot water extract significantly decreased the production of mIL-6, mNOS-II, mTNF-${\alpha}$, and increased the production of mIL-10, mIL-1 receptor antagonist. 2. The DDTCMT hot water extract significantly suppressed the production of IL-$1{\beta}$, IL-6, TNF-${\alpha}$ in BV2 microglial cell line treated by LPS. 3. The DDTCMT hot water extract significantly suppressed the NO and ROS production in BV2 microglial cell line treated by LPS. 4. The DDTCMT hot water extract groups showed inhibition of AChE activity in NGF treated PC-12 cell line. 5. The DDTCMT hot water extract suppressed anti-AChE expression in NGF treated PC-12 cell line was observed by Western blot analysis. 6. The DDTCMT hot water extract & ultra-fine powder groups showed significantly inhibitory effect on the scopolamine -induced impairment of memory in the experiment of Morris water maze. Conclusions : These results suggest that the DDTCMT hot water extract & ultra-fine powder may be effective for the prevention and treatment of Alzheimer's disease.

Effects of ChongMyung-Tang and ChongMyung-Tang added Moutan Cortex Hot water extract & Ultra-fine Powder on Microglia and Memory Deficit Model (총명탕(聰明湯)과 파극천총명탕(巴戟天聰明湯) 열수추출물, 초미세분말제형이 microglia 및 기억력 감퇴 병태모델에 미치는 영향)

  • Lim, Jung-Hwa;Jung, In-Chul;Lee, Sang-Ryong
    • Journal of Physiology & Pathology in Korean Medicine
    • /
    • v.20 no.4
    • /
    • pp.997-1008
    • /
    • 2006
  • This experiment was designed to investigate the effect of the CMT and PCMT hot water extract & ultra-fine powder on microglia and memory deficit model. The effects of the CMT and PCMT hot water extract on expression of $IL-l{\beta},\;IL-6,\;TNF-{\alpha}$, NOS-II, COX-2, IL-10, $TGF-{\beta}1$ mRNA and production of $IL-l{\beta},\;IL-6,\;TNF-{\alpha}$, NO, ROS in BV2 microglial cell line treated by lipopolysacchaide(LPS) , serum glucose, uric acid, AChE activity of the memory deficit mice induced by scopolamine , behavior of the memory deficit mice induced by scopolamine and were investigated, respectively. The CMT and PCMT hot water extract suppressed the expression of $IL-l{\beta},\;IL-6,\;TNF-{\alpha}$, NOS-11, COX-2 mRNA, production of $IL-l{\beta},\;IL-6,\;TNF-{\alpha}$, NO, ROS and increased the expression of IL-10, $TGF-{\beta}1$ mRNA in BV2 microglial cell line treated by LPS. The PCMT hot water extract & ultra-fine powder increased glucose, decreased uric acid and AChE significantly in the serum of the memory deficit mice induced by scopolamine. The CMT and PCMT hot water extract & ultra-fine powder groups showed significantly inhibitory effect on the scopolamine-induced impairment of memory in the experiment of Morris water maze. According to the above result, it is suggested that the CMT and PCMT hot water extract & ultra-fine powder might be usefully applied for prevention and treatment of dementia.