• Korean Journal of Microbiology
• /
• v.24 no.1
• /
• pp.7-11
• /
• 1986

We screened lysates of the laboratory strains of pseudomonads utilizing hydrocarbon by agarose gel electrophoresis and cesium chloride-ethidium bromide equilibrium centrifugation, to find an intrinsic plasmid as a vector and to examine the relationship between the plasmid and hydrocarbon degradation. Only one strain from the examined strains, Pseudomonas putida KU190, contained a plasmid. We named the plasmid pKU41. The molecular size of pKU41 was determined as 41kb, using covalently closed circular forms of RP4 and pSY343 as standard size markers. The restriction sites of pKU41 for BamHI, BglII, EcoRI, HindIII, and SalI were 3, 1, 3, 6 and more than 13, respectively. With double or triple digestion, restriction map of pKU41 was constructed for BamHI, BglII and HindIII. For elucidation on the biological function of the plasmid, test was conducted on the ability of hydrocarbon utilization of the host strain but no apparent relationship was observed.

• Proceedings of the Korean Society of Sericultural Science Conference
• /
• 1997.06a
• /
• pp.1-21
• /
• 1997

Silk is praised "Queen of Fiber", which wrote a glorious page in the history of human adornment culture. Thanks to the development of science and technology, silk production has been increased dramatically during last half century. Nowadays, as "Healthy Fiber" silk lets all the globe to be forming the "Silk Boom" Like in other scientific fields, a great deal of progress has been made in sericultural science and technology, which also accelerated the development of multipurpose utilization of sercultural recourses. The main tendency of the sericulture development is raising the quality of cocoon, and at the same time, increasing the output per unit mulberry area and labor production efficiency. The chief measure of raising the cocoon quality is breeding and selecting the new mulberry and silkworm varieties, preventing and curing the diseases and pests and improving the environment of mounting, and the symble for increasing the labor productivity is carrying out the technological system of saving labor and operation ease, and enlarging the production scale. As a natural protein fiber, silk will be still a favorite with human in the next century, because its structure and properties can not be replaced by other any fiber. The 21st century is the biological time, and measures of developing sericulture will use the achievement on the field of molecular biology and biotechnology to carry out the molecular breeding, development regulating, silkworm rearing industrializing, exploiting the new silk and its new usage, utilizing the sericultural resources, and producing the special protein from silkworm as a host of expressing exogenous genes so that make the sericulture to contribute more what it can do for the human being.

• Journal of Microbiology and Biotechnology
• /
• v.32 no.6
• /
• pp.681-698
• /
• 2022

The Nile tilapia Oreochromis niloticus, Atlantic salmon Salmo salar, rainbow trout Oncorhynchus mykiss, olive flounder Paralichthys olivaceus, common carp Cyprinus carpio, grass carp Ctenopharyngodon idella and rohu carp Labeo rohita are farmed commercially worldwide. Production of these important finfishes is rapidly expanding, and intensive culture practices can lead to stress in fish, often reducing resistance to infectious diseases. Antibiotics and other drugs are routinely used for the treatment of diseases and sometimes applied preventatively to combat microbial pathogens. This strategy is responsible for the emergence and spread of antimicrobial resistance, mass killing of environmental/beneficial bacteria, and residual effects in humans. As an alternative, the administration of probiotics has gained acceptance for disease control in aquaculture. Probiotics have been found to improve growth, feed utilization, immunological status, disease resistance, and to promote transcriptomic profiles and internal microbial balance of host organisms. The present review discusses the effects of single and multi-strain probiotics on growth, immunity, heamato-biochemical parameters, and disease resistance of the above-mentioned finfishes. The application and outcome of probiotics in the field or open pond system, gaps in existing knowledge, and issues worthy of further research are also highlighted.

• The Plant Pathology Journal
• /
• v.39 no.3
• /
• pp.275-289
• /
• 2023

Fungal isolates from infected Chinese quince trees were found to cause black rot in Yeongcheon, Gyeongsangbuk Province, Korea. The quince leaves withered and turned reddish-brown and fruits underwent black mummification. To elucidate the cause of these symptoms, the pathogen was isolated from infected leaf and fruit tissues on potato dextrose agar and Levan media. Several fungal colonies forming a fluffy white or dark gray mycelium and two types of fungi forming an aerial white mycelium, growing widely at the edges, were isolated. Microscopic observations, investigation of fungal growth characteristics on various media, and molecular identification using an internal transcribed spacer, β-tubulin, and translation elongation factor 1-α genes were performed. The fungal pathogens were identified as Diplodia parva and Diplodia crataegicola. Pathogenicity tests revealed that the pathogen-inoculated fruits exhibited a layered pattern, turning brown rotting; leaves showed circular brown necrotic lesions. The developed symptoms were similar to those observed in the field. Fungal pathogens were reisolated to fulfill Koch's postulates. Apples were inoculated with fungal pathogens to investigate the host range. Strong pathogenicity was evident in the fruits, with browning and rotting symptoms 3 days after inoculation. To determine pathogen control, a fungicidal sensitivity test was conducted using four registered fungicides. Thiophanate-methyl, propineb, and tebuconazole inhibited the mycelial growth of pathogens. To the best of our knowledge, this is the first report on the isolation and identification of the fungal pathogens D. parva and D. crataegicola from infected fruits and leaves of Chinese quince, causing black rot disease in Korea.

• Journal of Ginseng Research
• /
• v.48 no.2
• /
• pp.229-235
• /
• 2024

Background: Plant health is directly related to the change in native microbial diversity and changes in soil health have been implicated as one of the main cause of root rot. However, scarce information is present regarding allelopathic relationship of Panax notoginseng root exudates and pathogenic fungi Fusarium oxysporum in a continuous cropping system. Methods: We analyzed P. notoginseng root exudate in the planting soil for three successive years to determine phenolic acid concentration using GC-MS and HPLC followed by effect on the microbial community assembly. Antioxidant enzymes were checked in the roots to confirm possible resistance in P. notoginseng. Results: Total 29 allelochemicals in the planting soil extract was found with highest concentration (10.54 %) of p-hydroxybenzoic acid. The HPLC showing a year-by-year decrease in p-hydroxybenzoic acid content in soil of different planting years, and an increase in population of F. oxysporum. Moreover, community analysis displayed negative correlation with 2.22 mmol. L^-1 of p-hydroxybenzoic acid correspond to an 18.1 % population of F. oxysporum. Furthermore, in vitro plate assay indicates that medium dose of p-hydroxybenzoic acid (2.5-5 mmol. L^-1) can stimulate the growth of F. oxysporum colonies and the production of macroconidia, as well as cell wall-degrading enzymes. We found that 2-3 mmol. L^-1 of p-hydroxybenzoic acid significantly increased the population of F. oxysporum. Conclusion: In conclusion, our study suggested that p-hydroxybenzoic acid have negative effect on the root system and modified the rhizosphere microbiome so that the host plant became more susceptible to root rot disease.

• International conference on construction engineering and project management
• /
• 2024.07a
• /
• pp.909-916
• /
• 2024

The emergence of Building Information Modelling (BIM), reality data, Virtual Reality (VR), and Augmented Reality (AR) has significantly enhanced the collaboration between stakeholders in construction management. The utilization of VR/AR devices holds considerable potential for monitoring safety in complex and constrained working environments on the construction site. On the other hand, near-miss incidents remain an important early sign of struck-by accidents. However, research on early warning and prevention methods for this risk is still limited. This paper, therefore, presents a framework for on-site and off-site collaborative safety monitoring framework using augmented and virtual reality for near-miss incidents. In the proposed framework, three phases to develop a VR/AR-based safety monitoring system include (1) construction safety simulation environment, (2) localization-based interaction system, and (3) safety monitoring system. The system can undertake the processing of data and enables communication among disparate VR/AR devices. VR clients are observational tools and offer guidance, while the AR client stays onsite for construction tasks. All clients connect to a processing computer, which also works as a host. The system embedded in the AR device can trigger an alarm or receive signals from the VR client when a near-miss issue happens. Additionally, all device clients possess the capability to share data acquired from onsite monitoring cameras, thereby fostering effective discussions and decision-making. The efficacy of this cross-platform system has been validated through the implementation of an outdoor coordination case study.

• 대한공업교육학회지
• /
• v.32 no.1
• /
• pp.134-152
• /
• 2007

This study deals with the development of computer program which can restrict students to access to the unallowable web sites during the Internet based class. Our suggested program can find the student's access list to the unallowable sites, display it on the teacher's computer screen. Through the limitation of the student's access, teacher can enhance the efficiency of class and fulfill his educational purpose for the class. The use of our results leads to the effective and safe utilization of the Internet as the teaching tools in the class. Meanwhile, the typical method is to turn off the LAN (Local Area Network) power in order to limit the student's access to the unallowable web sites. Our program has been developed on the Linux operating systems in the small network environment. The program includes following five functions: the translation function to change the domain name into the IP(Internet Protocol) address, the search function to find the active students' computers, the packet snoop to capture the ongoing packets and investigate their contents, the comparison function to compare the captured packet contents with the predefined access restriction IP address list, and the restriction function to limit the network access when the destination IP address is equal to the IP address in the access restriction list. Our program can capture all passing packets through the computer laboratory in real time and exactly. In addition, it provides teacher's computer screen with the all relation information of students' access to the unallowable sites. Thus, teacher can limit the student's unallowable access immediately. The proposed program can be applied to the small network of the elementary, junior and senior high school. Our research results make a contribution toward the effective class management and the efficient computer laboratory management. The related researches provides teacher with the packet observation and the access limitation for only one host, but our suggested program provides teacher with those for all active hosts.

• Korean Journal of Soil Science and Fertilizer
• /
• v.19 no.4
• /
• pp.345-350
• /
• 1986

In order to select suitable strains for utilization as soybean inoculants, the affinity between Rhizobium japonicum strains isolated from the domestic field and the several soybean varieties being distributed as recommended species was investigated by cross-inoculation experiments. The results obtained are as follows: 1. Rhizobium japonicum R-214 and R-138 showed broad affinity range to varies soybean species in terms of the nodule forming ability, the nitrogen fixing activity and the amount of total nitrogen absorption. In contrast, Rhizobium japonicum R-224 showed very low affinity to the same soybean groups. 2. The affinity of Hwang-Keum Kong and Jang-Paeck Kong to the rhizobium strains was the most signigicant, and that of Jang-Yeop Kong, Dong-Buk Tae, Paeck-Woon Kong and Dan-Yeop Kong was also significant. On the other hand, Pang-Sa Kong showed low affinity to the strains. 3. In general, the strains having high nitrogen-fixing activity seemed to possess the broad affinity range to the soybean hosts.

• Journal of KIISE:Computer Systems and Theory
• /
• v.34 no.9
• /
• pp.425-434
• /
• 2007

Even though Internet backbone speeds have increased in the last few years due to projects like Internet 2 and NGI, many high performance distributed applications are able to achieve only a small fraction of the available bandwidth. The cause of such problem is due to a character of TCP/IP. The primary goal of this protocol is reliable data transmission. Therefore high speed data transmission didn't be considered when TCP/IP is designed. Hence several researchers have been studied in order to solve the problem of TCP/IP. One of these research results, parallel transfer technique, solves this problem to use parallel TCP connections on application level. Additionally, this technique is compatibility. Recently, these researchers have been studied a mechanism to decide the number of parallel TCP connections. However, some researchers reported the number of parallel TCP connection base on only empirical results. Although hardware performance of host affects transmission rate, the hardware performance didn't be considered in their works. Hence, we collect all data related to transmission rate, such as hardware state information (cpu utilization, interrupt, context switch). Then, we analyzed collected data. And, we suggest a new mechanism determining number of parallel TCP connections for maximization of performance based on our analysis.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.21 no.8
• /
• pp.18-25
• /
• 2020

With the development of water internet technology, data communication between objects is expanding. Research related to data communication technology between vehicles that incorporates related technologies into vehicles has been actively conducted. For data communication between mobile terminals, data stability, reliability, and real-time performance must be guaranteed. The 5 GHz Wi-Fi band, which is advantageous in bandwidth, communications speed, and wireless saturation of the wireless network, was selected as the data communications network between vehicles. This study analyzes how to design and implement a 5 GHz Wi-Fi network in a vehicle network. Considering the characteristics of the mobile communication terminal device, a continuous variable communications structure is proposed to enable high-speed data switching. We simplify the access point access procedure to reduce the latency between wireless terminals. By limiting the Transmission Control Protocol Internet Protocol (TCP/IP)-based Dynamic Host Configuration Protocol (DHCP) server function and implementing it in a broadcast transmission protocol method, communication delay between terminal devices is improved. Compared to the general commercial Wi-Fi communication method, the connection operation and response speed have been improved by five seconds or more. Utilizing this method can be applied to various types of event data communication between vehicles. It can also be extended to wireless data-based intelligent road networks and systems for autonomous driving.