• The Plant Pathology Journal
• /
• v.19 no.3
• /
• pp.162-165
• /
• 2003

Transformation of Fuji apple (Malus domestica 'Fuji') was performed using Agrobacterium tumefaciens harboring a coat protein (CP) gene of Cucumber mosaic virus (CMV). A plasmid DNA containing the virus CP and NPT II genes was introduced into the loaves of apple by th e Agrobacterium - mediated transformation procedure. Regenerated transformants of the apple were obtained by kanamycin resistance conferred by the introduced NPT II gene. PCR analysis showed that 3 out of 20 putatively selected R0 plant lines contain the CMV-CP gene. Nine putative transgenic lines out of 20 lines were investigated with the PCR analysis; 5 regenerants produced a 450 bp DNA band and 3 regenerants showed a 671 bp DNA band for the NPT II and CMV-CP genes, respectively. Southern hybyidization results demonstrate the successful integration of the CMV-CP gene into the genome of the apple. This is the first report on the generation of useful vius resistance source of transgenic apple for molecular breeding program.

• Journal of Plant Biotechnology
• /
• v.43 no.2
• /
• pp.181-188
• /
• 2016

In this study, we developed 15 novel polymorphic simple sequence repeat (SSR) markers by SSR-enriched genomic library construction from Codonopsis lanceolata. We obtained a total of 226 non-redundant contig sequences from the assembly process and designed primer sets. These markers were applied to 53 accessions representing the cultivated C. lanceolata in South Korea. Fifteen markers were sufficiently polymorphic, and were used to analyze the genetic relationships between the cultivated C. lanceolata. One hundred three alleles of the 15 SSR markers ranged from 3 to 19 alleles at each locus, with an average of 6.87. By cluster analysis, we detected clear genetic differences in most of the accessions, with genetic distance varying from 0.73 to 0.93. Phylogenic analysis indicated that the accessions that were collected from the same area were distributed evenly in the phylogenetic tree. These results indicate that there is no correlative genetic relationship between geographic areas. These markers will be useful in differentiating C. lanceolata genetic resources and in selecting suitable lines for a systemic breeding program.

• Horticultural Science & Technology
• /
• v.30 no.4
• /
• pp.400-408
• /
• 2012

Effects of initial controlled atmosphere (CA) treatment on quality maintenance of 'Fuji' apples were assessed and compared with 1-methylcyclopropene (1-MCP) treatment and continuous CA storage. Apples were harvested twice at different maturity, treated with 1 ${\mu}L{\cdot}L^{-1}$ 1-MCP and then stored for 8 months at $0^{\circ}C$ under 3 conditions: air, CA for the first month followed by air (initial CA), and continuous CA (full CA). CA storage was performed with 1.5 kPa $O_2$ and < 1.0 kPa ($N_2$ balance). Following long-term storage, export simulation, refrigerated shipment and local distribution, were performed by holding apples at $0^{\circ}C$ for 2 weeks and on the shelf at $20^{\circ}C$ for 7 days. Both the application of 1-MCP and CA storage reduced ethylene production and respiration rates. Initial CA storage was also effective on reducing the metabolism although the effects were not as noticeable as full CA. Full CA storage with or without 1-MCP treatment maintained titratable acidity, flesh firmness, and sensory quality at the acceptable to excellent level even after the export simulation following 8-month storage regardless of harvest maturity. In contrast, effects of initial CA storage were limited to the maintenance of firmness and texture in early-harvested apples. Overall results indicated that harvest maturity is the critical factor for export fruit quality after long-term storage when separate treatment of initial CA storage or 1-MCP treatment is applied as a postharvest program.

• Mycobiology
• /
• v.39 no.3
• /
• pp.200-205
• /
• 2011

Marssonina coronaria associated with apple blotch disease causes severe premature defoliation, and is widely distributed in Korea. Thirteen isolates were collected from orchards located in Gyeongbuk Province from 2005~2007. All isolates displayed over 99.6% and 99.2% sequence similarity to each other in internal transcribed spacer regions and partial sequences of 28S rDNA, respectively. The isolates were phylogenetically closely related to Chinese isolates. Selected isolates did not differ in their pathogenicity. The optimum conditions for fungal growth were $20^{\circ}C$ and pH 6 on peptone potato dextrose agar (PPDA). Peptone and mannose were the best nitrogen and carbon source, respectively. Fungal growth was better on PPDA than on common potato dextrose agar. This study provides valuable information for integrated disease management program and facilitates the routine culturing of M. coronaria.

• Journal of Korean Society of Rural Planning
• /
• v.25 no.4
• /
• pp.87-97
• /
• 2019

본 연구는 방과 후 농업 체험활동 프로그램이 초등학생의 정서지능과 회복탄력성 향상 및 우울감 감소에 미치는 영향을 알아보고자 실시하였다. 서울시 초등학교에 재학 중인 4-6학년(11-13세) 초등학생 총 30명이 본 연구에 참여하였다. 참여에 동의한 30명의 학생 중 농업체험 프로그램에 참여를 희망한 18명은 실험군으로, 농업체험 프로그램에 참여를 희망하지 않은 학생 12명은 대조군으로 집단화 하였다. 실험군은 2018년 4월부터 7월까지, 총 12주간(주 1회, 회기당 60분)의 방과 후 활동으로 본 프로그램에 참여하였다. 초등학생의 정서적 건강 증진을 위해 사회정서학습 이론에 기반한 농업체험 프로그램을 설계하였다. 프로그램 실시 전과 후에 자기보고식 설문지를 이용해 정서지능, 우울, 회복탄력성을 측정하였다. 그 결과, 농업 체험활동 프로그램에 참여한 초등학생의 정서지능이 향상되었고(P=0.031), 우울감이 감소된(P=0.011) 반면 대조군에서는 유의한 차이가 나타나지 않았다(P>0.05). 회복탄력성은 실험군과 대조군에서 모두 실험 전후 유의한 차이가 없었다(P>0.05). 본 농업체험 프로그램에 참여한 아동들은 프로그램에 대해 높은 만족감을 보고하였다. 따라서 본 연구에서 사회 정서학습이론을 적용한 농업체험 프로그램이 초등학교 고학년 학생의 정서지능 향상과 우울감 감소에 효과적인 것으로 나타났다. 추후, 표본수를 확대하여 농업 체험활동 프로그램의 효과 검증 연구가 추가적으로 필요할 것이라 생각되며, 다양한 지역의 초등학생들에게 확대 적용하는 연구도 필요할 것이다.

• Journal of Mushroom
• /
• v.17 no.4
• /
• pp.171-178
• /
• 2019

Agaricus bisporus is a functional food and among the most widely cultivated mushrooms in the world. In this study, we analyzed the genetic diversity and population structure of 23 Korean and 42 foreign A. bisporus cultivars using SSR (Simple sequence repeat) markers. Genetic diversity of A. bisporus cultivars was as follows: number of alleles was approximately 13; observed and expected heterozygosity were approximately 0.59 and 0.74, respectively; and polymorphic information content value was about 0.71. A. bisporus cultivars were divided into three groups using distance-based analysis. Genetic diversity of Group 2, which consisted of cultivars from various countries, was high. In addition, model-based subpopulations were divided into two, and the genetic diversity of Pop2 (Population 2), which had many cultivars, was high. The results of this study could be used in a breeding program for A. bisporus, such as the development of new genetic resources and securing diversity.

• Horticultural Science & Technology
• /
• v.29 no.3
• /
• pp.224-231
• /
• 2011

Effects of 1-methylcyclopropene (1-MCP) treatment and controlled atmosphere (CA) storage was analyzed for the export quality of 'Fuji' apples after 8-month storage. Apples were harvested from two orchards located in different regions at relatively late stage of maturity for long-term storage. Fruit were treated with 1 ${\mu}L{\cdot}L^{-1}$ 1-MCP and then stored in $0^{\circ}C$ air or CA with 1.5 kPa $O_2$ and < 1.0 kPa $CO_2$ for 8 months. To simulate poststorage export process such as 2-week refrigerated container shipment plus 7-day local distribution, fruit were put in $0^{\circ}C$ air storage for additional two weeks and then on the shelf for 7 days at $20^{\circ}C$. Both the 1-MCP treatment and CA storage reduced respiration and ethylene evolution after storage and shipping simulation, and successfully maintained titratable acidity and flesh firmness even after the export process. In 'Fuji' apples harvested after adequate stage of maturity for long-term storage, however, 1-MCP treatment alone seemed not to be satisfactory for the maintenance of sensorial fruit texture for export market. CA storage or 1-MCP treatment/CA storage combination program should be applied for lately harvested 'Fuji' apples exported after eight months storage.

• Korean Journal of Microbiology
• /
• v.54 no.1
• /
• pp.69-70
• /
• 2018

Erwinia pyrifoliae is a Gram-negative bacterium causing black shoot blight in apple and Asian pear trees. E. pyrifoliae strain EpK1/15 was isolated in 2014 from an apple twig from the Pocheon, Gyeonggi-do, South Korea. In this study, we report the draft genome sequence of E. pyrifoliae EpK1/15 using PacBio RS II platform. The draft genome is comprised of a circular chromosome with 4,027,225 bp and 53.4% G + C content and a plasmid with 48,456 bp and 50.3% G + C content. The draft genome includes 3,798 protein-coding genes, 22 rRNA genes, 77 tRNA genes, 13 non-coding RNA genes, and 231 pseudo genes.

• Journal of People, Plants, and Environment
• /
• v.23 no.6
• /
• pp.683-694
• /
• 2020

Background and objective: Cancer is the number one cause of death in Korea, and it affects any part of the body regardless of gender and age. Forest healing is a treatment that maximizes the effect of treatment and improves the quality of life. This study aims to provide basic data for the development and implementation of differentiated forest healing programs for cancer patients based on the survey on their interest and needs for the programs. Methods: The subjects were those diagnosed with cancer from October 2018 to April 2019, and this study used 144 copies of the questionnaire retrieved. The sample size of this study (n = 144) was the appropriate size required by G-Power, and the collected responses were analyzed using SPSS 25.0. Results: In the frequency analysis on the interest in forest healing, 79.2% of the subjects had no experience participating in forest healing, but 87% were aware of it, and 82.6% showed the intention to participate in forest healing programs. This indicates that even though not many of them have experience participating in forest healing, they showed high interest and needs for participation. They preferred to participate in spring (29.9%) and fall (27.8%), in programs carried out for 1.5-2 hours in the morning on weekdays. Conclusion: This study has implications for the analysis on forest healing needs of cancer patients, and it is necessary to plan, develop, and implement differentiated forest healing programs that meet the needs of the cancer patients depending on their characteristics. There is also a need to plan forest healing program that can promote both psychological stability and physical health of cancer patients and verify and evaluate their effects based on specialized training of forest healing instructors.

• Horticultural Science & Technology
• /
• v.31 no.5
• /
• pp.544-551
• /
• 2013

The effect of different silicon (Si) sources and methods of application on the growth of two chrysanthemum cultivars grown in a soilless substrate was investigated. Rooted terminal cuttings of Dendranthema grandiflorum 'Lemmon Eye' and 'Pink Eye' were transplanted into pots containing a coir-based substrate. A nutrient solution containing 0 or $50mg{\cdot}L^{-1}$ Si from calcium silicate ($CaSiO_3$), potassium silicate ($K_2SiO_3$) or sodium silicate ($Na_2SiO_3$) was supplied once a day through an ebb-and-flood sub irrigation system. A foliar spray of 0 or $50mg{\cdot}L^{-1}$ Si was applied twice a week. Cultivar and application method had a significant effect on plant height. Cultivar, application method, and Si source had a significant effect on plant width. Of the three Si sources studied, $K_2SiO_3$ was found to be the best for the increasing number of flowers, followed by $CaSiO_3$ and $Na_2SiO_3$. In both the cultivars, sub irrigational supply of Si developed necrotic lesions in the older leaves at the beginning of the flowering stage as compared to the control and foliar spray of Si. Cultivar, application method, Si source, and their interactions had significant influence on leaf tissue concentrations of calcium (Ca), potassium (K), phosphorus (P), magnesium (Mg), sulfur (S), sodium (Na), boron (B), iron (Fe), and zinc (Zn). The addition of Si to the nutrient solution decreased leaf tissue concentrations of Ca, Mg, S, Na, B, Cu, Fe, and Mn in both cultivars. The greatest Si concentration in leaf tissue was found in 'Lemmon Eye' ($1420{\mu}g{\cdot}g^{-1}$) and 'Pink Eye' ($1683{\mu}g{\cdot}g^{-1}$) when $K_2SiO_3$ was applied through a sub irrigation system and by foliar spray, respectively.