• 농업과학연구
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• 제41권4호
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• pp.299-302
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• 2014

A freesia (Freesia hybrida Hort.) 'Shiny Bell' was developed for the cut flower in the National Institute of Horticultural Herbal Science in 2012. This hybrid was selected from a crossing between cn. 'Teresa' and 'Rossini', which is white semi-double flowering and red double flowering, respectively. in 2004. Morphological characteristics of the selected hybrid were investigated for 3 years from 2009 to 2011, and then it was named 'Shiny Bell'. 'Shiny Bell' had red color of RHS W155C, and double flower. The growth of the plant was vigorous and the average height was 109.3 cm. The average flower width was 5.8 mm, number of floret per stalk was 11.3, and stalk length was 8.5 cm. The average yield, 7.3 Branches per plant, was 3 stems per plant more than the control cultivar. The average days to first flowering of 'Shiny Bell', was 143.3 days, and it was approximately 7 days later than the control cultivar. The average yield of 'Shiny Bell' was 4.0 cormlets per plant.

• Journal of Plant Biotechnology
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• 제43권1호
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• pp.132-137
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• 2016

The genus Cypripedium, known as lady's slipper orchid, has been considered to have a considerable marketability as a potted and garden plant with beautiful flowers; however, this species is becoming endangered and, in some places extinct, due to habitat destruction and illegal collections. As such, an optimized artificial propagation system is necessary for its conservation and horticultural cultivation. For the establishment of the in vitro proliferation of the endangered Cypripedium macranthos Sw., native to Korea, the effects of medium strength and sucrose concentrations on germination, protocorm formation and seedling growth were investigated through asymbiotic seed culture. The highest germination rate and protocorm formation rate were obtained with a 1/4 MS medium; higher MS medium strengths did not generate the favorable conditions required for germination and protocorm growth. The overall growth characteristics of roots and buds developed from protocorms were best in cultures of 1/4 MS medium. On this medium containing $10g{\cdot}L^{-1}$ sucrose, both the germination rate and protocorm formation rate increased significantly. The general growth properties of seedlings developed on the medium with $10g{\cdot}L^{-1}$ sucrose were best, showing the highest bud formation rate and root number. Our results demonstrate that the 1/4 MS medium, supplemented with $10g{\cdot}L^{-1}$ sucrose, could improve in vitro germination, and facilitate the growth of seedlings developed from the protocorms of C. macranthos Sw.

• Journal of Plant Biotechnology
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• 제43권3호
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• pp.311-316
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• 2016

The efficacy of plant breeding has been enhanced by application of molecular markers in population screening and selection. Pepper (Capsicum annuum L.) is a major staple crop that is economically important with worldwide distribution. It is valued for its spicy taste and medicinal effect. The aim of this study was to discover single nucleotide polymorphisms (SNPs), microsatellite markers information, and percentage sharing through orthologous analysis of pepper-specific pungency-related genes. Here, we report the results of transcriptome analysis and microsatellite markers for four pepper varieties that possess a pungency-related gene. Orthologous analyses was performed to identify species-specific pungency-related genes in pepper, Arabidopsis thaliana L., potato (Solanum tuberosum L.), and tomato (Solanum lycopersicum L.). Advancements in next-generation sequencing technologies enabled us to quickly and cost-effectively assemble and characterize genes to select molecular markers in various organisms, including pepper. We identified a total of 9762, 7302, 8596, and 6886 SNPs for the four pepper cultivars Blackcluster, Mandarine, Saengryeg 211, and Saengryeg 213, respectively. We used 454 GS-FLX pyrosequencing to identify microsatellite markers and tri-nucleotide repeats (54.4%), the most common repeats, followed by di-, hexa-, tetra-, and penta-nucleotide repeats. A total of 5156 (15.9%) pepper-specific pungency-related genes were discovered as a result of orthologous analysis.

• 식물병연구
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• 제22권3호
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• pp.213-216
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• 2016

토마토반점위조바이러스(TSWV)는 토마토 식물체에 가장 심한 피해를 주는 바이러스들 중의 한 종이다. 2015년에 선단 부위가 고사되는 토마토 식물체의 잎에서 분리한 TSWV-GT를 토마토 품종들에 대한 내병성 검정에 활용하였다. 우리나라에서 시판되고 있는 토마토 51품종 중 'TY 스마트사마'와 '마놀리아'는 내병성, '티티찰', 'TY 센스큐' 및 '베네키아'는 중간내병성이었다.

• 원예과학기술지
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• 제32권5호
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• pp.584-589
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• 2014

Jeffersonia dubia is a spring-flowering perennial found in rich forests in Korea and Northern China and has potential as an ornamental or medicinal plant. However, illegal picking and land use change have decreased the number of populations and overall population size of this plant in its natural habitat. Although J. dubia has been reported to be a shade-preferring plant, no study has determined the optimum light intensity for its growth. The objectives of this work were to observe the effects of various shading levels on the physiological responses of J. dubia and to determine the proper shading level for cultivation. Treatments consisted of four shading levels (0%, 50%, 75%, and 95% shade) imposed using black mesh cloth. The number of leaves and dry weight increased with decreased shading. The shoot-to-root ratio increased with increased shading, mainly due to decreased root dry weight under shading. Plants showed low net $CO_2$ assimilation rates and $F_v/F_m$ values combined with low dry matter levels when grown under 0% shade (full sunlight). These results indicate that J. dubia plants experience excessive irradiance without shading, resulting in damage to the photosynthetic apparatus. By contrast, the net photosynthesis rate increased as the shading level increased. $F_v/F_m$, the potential efficiency of PSII, was 0.8 under 95% shade, indicating that J. dubia is well-adapted under heavy shading. However, the low dry matter of plants in the 95% shade treatment indicated that the low light intensity under 95% shade led to a decline in plant growth. Thus, moderate light (50% shading) is recommended for cultivating J. dubia without physiological defects.

• 식물병연구
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• 제21권3호
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• pp.180-185
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• 2015

In the present work, a pair of primers specific to Tomato yellow leaf curl virus (TYLCV) was designed to allow specific amplification of DNA fragments from any TYLCV isolates using an extensive alignment of the complete genome sequences of TYLCV isolates deposited in the GenBank database. A pair of primers which allows the specific amplification of tomato ${\beta}$-tubulin gene was also analyzed as an internal PCR control. A duplex PCR method with the developed primer sets showed that TYLCV could be directly detected from the leaf crude sap of infected tomato plants. In addition, our developed duplex PCR method could determine PCR errors for TYLCV diagnosis, suggesting that this duplex PCR method with the primer sets is a good tool for specific and sensitive TYLCV diagnosis. The developed duplex PCR method was further verified from tomato samples collected from some farms in Korea, suggesting that this developed PCR method is a simple and reliable tool for rapid and large-scale TYLCV detections in tomato plants.

• 한국자원식물학회지
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• 제27권6호
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• pp.622-628
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• 2014

The aim of this work is to evaluate the potential of oriental melon (Cucumis melo L. var. makuwa Makino) seeds for the management of type 2 diabetes by controlling glucose absorption. The ${\alpha}$-glucosidase and ${\alpha}$-amylase inhibitory effects of the hexane extracts from oriental melon seeds were investigated. A bioassay-guided fractionation technique was used to elucidate the principal active components. The results show that the hexane extract from oriental melon seeds exhibited high inhibitory activities against ${\alpha}$-glucosidase and ${\alpha}$-amylase. The hexane extract was further fractionated into four sub-fractions. Among them, the sub-fraction F-1 exhibited the most potent anti-diabetic effect. The active components were isolated and identified by gas chromatography/mass spectroscopy (GC-MS). Free fatty acids showed significant hypoglycemic activity (p < 0.001) and fatty acid composition influenced enzyme inhibitory activities. These results suggest that oriental melon seeds could be used to prevent type 2 diabetes.

• 한국육종학회지
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• 제41권3호
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• pp.358-362
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• 2009

A New Cymbidium variety "Yellow Evening" was developed from a cross between "Oji" and "Valley Flower Cherry Life" followed by seedling and line selections at the National Institute of Horticultural and Herbal Science (NIHHS), Rural Development Administration (RDA) in 2004. After investigation of the characteristics and selection for 9 years (1996-2004), it has been selected specifically pot orchid flower. "Yellow Evening" has medium plant height and semi-erect peduncle. It has medium flowering habit and brownish yellow flowers (RHS, Y11B: petal and sepal) with grayed purple GP183A) spot on lip, when fully opened. Each peduncle has 15.3 flowers with diameter of about 7.4 cm. It has adequate peduncle height, with some curved and spreading petal and sepals. Under optical culture conditions, it started to bloom early-winter (November).

• The Plant Pathology Journal
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• 제37권6호
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• pp.681-686
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• 2021

It is difficult to distinguish melanose and melanoses-like symptoms with the naked eye because they appear similar. To accurately detect melanose symptoms caused by Diaporthe citri from melanose-like symptoms, we developed PCR-based specific primers Dcitri by aligning the internal transcribed spacer (ITS) region of D. citri with the ITS of Diaporthe cytosporella, Diaporthe foeniculina, Colletotrichum gloeosporioides, Botrytis cinerea, Alternaria citri, and Fusarium oxysporum found on citrus peel. PCR results showed that the specific product was amplified in D. citri but not in other isolates including, C. gloeosporioides, B. cinerea, A. citri, F. oxysporum. In addition, specific products were observed in melanose symptoms caused by D. citri but not in melanose-like symptoms, such as copper-injury, sunscald, damages by yellow tea thrips, and pink citrus rust mite. Using the Dcitri primers developed in this study, it is expected that melanose caused by D. citri could be accurately distinguished from melanose-like symptoms.

• 식물병연구
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• 제29권1호
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• pp.82-87
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• 2023

Exogenous ferrous chloride (FeCl₂) suppressed in vitro growth of Ralstonia pseudosolanacearum, causing bacteria for tomato bacterial wilt. More than 50 μM of FeCl₂ reduced the in vitro bacterial growth in dosedependent manners. Two to 200 μM of FeCl₂ did not affect the fresh weight of detached tomato leaves at 3 and 5 days after the petiole dipping without the bacterial inoculation. The bacterial wilt of the detached tomato leaves was evaluated by inoculating two different inoculum densities of R. pseudosolanacearum (10⁵ and 10⁷ cfu/ml) in the presence of FeCl₂. Bacterial wilt in the detached leaves by 10⁵ cfu/ml was efficiently attenuated by 10-200 μM of FeCl₂ at 3 and 5 days post-inoculation (dpi), but bacterial wilt by 10⁷ cfu/ml was only reduced by 200 μM of FeCl₂ at 3 and 5 dpi. These results suggest that iron nutrients can be included in the integrated disease management of tomato bacterial wilt.