• Research in Plant Disease
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• v.15 no.3
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• pp.262-265
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• 2009

The fungus gnat, Bradysia difformis was examined for its ability to transmit Fusarium oxysporum in PDA culture. Larvae and adults of B. difformis were able to transmit the fungus as ingested and sticking. We constructed GFP-expressed mutants with Fusarium oxysporum, then feed it to larvae of fungus gnat, B. difformis. So that mycelia were placed in the alimentary canal of larva.

• The Plant Pathology Journal
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• v.18 no.5
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• pp.244-250
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• 2002

Grapevine leafroll-associated 3 virus (GLRaV-3) and Grapevine fanleaf virus (GFLV) are important viral diseases of grapevine in the world. In this study, the most reliable woody indicator plants were selected for virus indexing. Two grapevines, LN33 (Couderc 1613x vitis berlandieri) and Vitis riparia Gloire, were selected for CLRaV-3 and CFLV graft indexing, respectively. The specific characteristics of Closterovirus isolated from grapevines cultivated in Korea were identified. filamentous virus-like particles only existed in the phloem parenchyma cell. In particular, the vesiculation of mitochondria was observed. This mitochondrial vesicu-lation was considered to be one of the most reliable cytopathic features of Closterovirus. During observation of GFLV-infected Chenopodium quinoa sections, virus-like particles arranged consistently were found forming several layers in cytoplasm. Moreover, virus-like particles in tubules were observed and were associated with plasmodesmata in cytoplasm. This is the first report on cytopathological characteristics of Closterovirus and Nepovirus identified from grapevines in Korea.

• Research in Plant Disease
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• v.22 no.3
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• pp.194-197
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• 2016

Chinese yam necrotic mosaic virus (CYNMV) is one of the most widespread viruses in Chinese yam (Dioscorea opposita Thunb.) and causes serious yield losses. Currently, genetic information of CYNMV is very restricted and complete genome sequences of only two isolates (one from Japan and another from China) have been reported. In this study, we determined complete genome sequence of the CYNMV isolate AD collected from Andong, Korea. Genetic analysis of the polyprotein amino acid sequence revealed that the Korean isolate AD has high similarity with the Japanese isolate PES3 (97%) but relatively low similarity with the Chinese isolate FX1 (78%). Phylogenetic analysis using the CYNMV 3' proximal nucleotide sequences harboring the coat protein and 3' untranslated region further supported genetic relationship among the CYNMV isolates. Based on comparative analysis of the CYNMV genome sequences determined in this study and other previous studies, we generated molecular detection primers that are highly specific and efficient for CYNMV diagnosis.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.10a
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• pp.15-15
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• 2019

The walnut (Juglans regia L.), a member of the Juglandaceae, is native to the mountain ranges of central Asia. This species of walnut is valued commercially for its nuts and in some areas for its timber. The seeds of walnut are recalcitrant and it has strong integument dormancy and their germination is irregular, making its natural propagation difficult. Low percentage of seed germination and long propagation cycle are the main problems of propagation. This study was conducted medium composition on in vitro plantlet regeneration from axillary buds of walnut. It has proved to be the most generally applicable and reliable method of in vitro propagation. Micropropagation culture that axillary buds are excised aseptically enables faster multiplication of plants. The axillary buds of walnut new cultivar "Sinlyeong" were cultured on two basal media which contained the different plant growth regulators depending on the respective shooting and rooting stage. After 12 weeks, the shoot generation rate was 85.3%, the shoot number and its length were 1.9/explant and 2.7 cm in the most favorable medium composition. The percentage of rooting was 25.4%. From these results, it was found the optimum basal medium and plant growth regulator for in vitro plant regeneration from axillary buds of walnut new cultivar "Sinlyeong". However, we have continued to search the other medium additives to enhance the rate of walnut root.

• Journal of People, Plants, and Environment
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• v.24 no.2
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• pp.137-152
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• 2021

Background and objective: This study was carried out to investigate and analyze the preference for plant fragrance and the psychological effects of a agro-healing activity in 101 firefighters aged 39.71 ± 10.94. Methods: For the psychological evaluation, post-traumatic stress symptoms, Korean occupational stress scale short form (KOSS-SF), emotional intelligence, and job satisfaction were surveyed on the Likert scale. For the preference for plant fragrances, 20 ㎕ of commercially available essential oils from four plant species (Mentha spp., Lavandula spp., Citrus limon, C. sinensis) were absorbed into each test paper and provided to the participants. Results: The rate of post-traumatic stress symptoms was high at 37.6% of the firefighters surveyed.. C. limon fragrance had the highest preference among the four plant fragrances, followed by C. sinensis > Mentha spp. > Lavandula spp. with statistically significant difference (F = 14.256, p < .001). The lower their age, income, and position, and higher the education level, the higher the preference for plant fragrances. And the administrative group had higher preference for plant fragrances than field group. In addition, as a result of examining the correlation between job satisfaction, preference for plant fragrance, post-traumatic stress, emotional intelligence, and job stress for fire-fighters, the higher the job satisfaction, the higher the fragrance preference and emotional intelligence, and the lower the post-traumatic stress and job stress. Conclusion: Therefore, installing a garden using botanical fragrances at workplaces that firefighters can easily access will improve job satisfaction and relieve stress. It can be concluded that applying the fragrance of plants to the healing farming activities at the fire-fighter's workplace can increase the usefulness of the healing activities.

• Journal of Plant Biotechnology
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• v.50
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• pp.183-189
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• 2023

This study investigated the impact of two distinct MS basal media: one containing FeNaEDTA and the other FeEDDHA, on the growth of five unique gerbera cultivars (Shy Pink, Pink Holic, Breeze, Harmony, Snow Dream). Notably, the response to these media types varied significantly among the cultivars, particularly concerning leaf yellowing and plant growth. 'Shiny Pink' and 'Pink Holic' exhibited leaf yellowing on the FeNaEDTA-containing medium but displayed leaf greening on the FeEDDHA-containing medium. In contrast, 'Snow Dream,' 'Harmony,' and 'Breeze' remained unaffected on both medium types. However, the FeNaEDTA-containing medium promoted higher plant height and petiole length in 'Breeze,' 'Harmony,' and 'Snow Dream' than the FeNaEDTA-containing medium did. A promotive effect of silver nanoparticles (AgNPs) on plant growth and leaf greening was observed in 'Pink Holic,' particularly on the FeNaEDTA-containing medium, while the addition of AgNPs to the FeEDDHA-containing medium negatively affected plant growth. These results highlight the substantial influence of medium type, specifically the presence of FeNaEDTA or FeEDDHA, on gerbera growth responses, emphasizing the critical role of medium selection in gerbera propagation. Additionally, when contemplating the addition of AgNPs for in vitro gerbera propagation, it is crucial to consider the medium type.

• Research in Plant Disease
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• v.21 no.4
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• pp.341-345
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• 2015

Virus-like symptoms including stunt, severe mosaic with malformation of leaves, fern-like leaves and abnormal petals were observed from an African impatiens (Impatiens walleriana) grown in a plant nursery in Icheon, Korea. Serological analysis using immuno-strip kits for viruses reported in African impatiens indicated that Cucumber mosaic virus (named CMV-Im) was a causal agent for the symptomatic African impatiens. Biological properties of CMV-Im were analyzed using responses of host plant species, suggesting that CMV-Im is a typical strain that belongs to CMV subgroup I. RT-PCR analysis verified CMV-Im infection from naturally infected African impatiens or mechanically inoculated some host species. Analysis of multiple alignments of CMV capsid protein (CP) sequences showed that CMV-Im shared high CP amino acids identities with other CMV strains. Phylogenetic tree analysis for the CP sequences of CMV-Im and representative CMV strains confirmed that CMV is a typical member of CMV subgroup I. To our knowledge, it is the first report of CMV in African impatiens in Korea.

• 한국약용작물학회:학술대회논문집
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• 2011.04a
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• pp.152-153
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• 2011

• 한국약용작물학회:학술대회논문집
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• 2011.04a
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• pp.272-273
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• 2011

• Research in Plant Disease
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• v.16 no.3
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• pp.320-322
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• 2010

The Sclerotium blight was found on Neofinetia falcata at Yong-in city, Gyenggi-do, Korea. The symptom occurred low leaves yellowish and wilt of a whole plant. Severely infected plants were blighted and dies eventually. White mycelial mats appeared on the surface of basal stem and bulbs and the sclerotia were formed on stems, roots, and sphagnum moss. The sclerotia were spherical in shape, 1~3 mm in size and white to brown in color. The optimum temperature for the growth and sclerotia formation was $25{\sim}30^{\circ}C$ on PDA. On the pathogenicity test, the first symptom was appeared 5 days after inoculation and development to severe stem rot and blight. The causal fungus was identified as Sclerotium rolfsii and we suggested to call that the new Sclerotium blight on Neofinetia falcata caused by Sclerotium rolfsii in Korea.