• 한국식품저장유통학회지
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• 제20권5호
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• pp.720-726
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• 2013

본 연구는 배 과즙의 가공조건을 최적화하기 위해 여과, 청징 및 갈변 방지 방법 및 가공 조건에 따른 항산화능을 조사하였다. 배를 분쇄 착즙 후 면포여과, 감압여과 및 원심분리를 실시한 결과 원심분리(3000 rpm, 10분간)를 할 경우 colorimetry value가 높은 'L'값(34.31)과, 낮은 'a'값(-0.13)을 보여 가장 좋은 청징 효과를 나타냈으며, 그 다음으로 감압여과(31.8%) 및 면포여과(11.0%) 순이었다. 청징제 종류에 따른 청징 효과는 1%의 gelatin이나 bentonite가 우수하였으며, tannin과 egg albumin은 효과가 없었다. 갈변저해제 처리에 따른 갈색도 측정결과 0.1% L-ascorbic acid 처리 시 11.5%의 갈변억제 효과를 보였다. 유리당은 fructose, sucrose, glucose, sorbitol이 검출되었으며, 가열배즙에서 fructose와 glucose는 증가되는 반면 sucrose는 감소되었다. 가공 조건에 따른 항산화능 조사 결과 총폴리페놀 함량은 가열배즙이 청징배즙에 비해 유의하게 많아졌으나 총플라보노이드 함량은 청징배즙과 가열배즙이 유사하게 증가하였다. DPPH radical 소거활성과 아질산염 소거능은 가열배즙과 청징배즙에서 착즙액보다 유의적으로 높은 것을 확인할 수 있었다.

• Journal of Microbiology and Biotechnology
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• 제32권2호
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• pp.187-194
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• 2022

Two α-ʟ-arabinofuranosidases (BfdABF1 and BfdABF3) and a β-ᴅ-xylosidase (BfdXYL2) genes were cloned from Bifidobacterium dentium ATCC 27679, and functionally expressed in E. coli BL21(DE3). BfdABF1 showed the highest activity in 50 mM sodium acetate buffer at pH 5.0 and 25℃. This exo-enzyme could hydrolyze p-nitrophenyl arabinofuranoside, arabino-oligosaccharides (AOS), arabinoxylo-oligosaccharides (AXOS) such as 3²-α-ʟ-arabinofuranosyl-xylobiose (A³X), and 2³-α-ʟ-arabinofuranosyl-xylotriose (A²XX), whereas hardly hydrolyzed polymeric substrates such as debranched arabinan and arabinoxylans. BfdABF1 is a typical exo-ABF with the higher specific activity on the oligomeric substrates than the polymers. It prefers to α-(1,2)-ʟ-arabinofuranosidic linkages compared to α-(1,3)-linkages. Especially, BfdABF1 could slowly hydrolyze 2³,3³-di-α-ʟ-arabinofuranosyl-xylotriose (A²⁺³XX). Meanwhile, BfdABF3 showed the highest activity in sodium acetate at pH 6.0 and 50℃, and it has the exclusively high activities on AXOS such as A³X and A²XX. BfdABF3 mainly catalyzes the removal of ʟ-arabinose side chains from various AXOS. BfdXYL2 exhibited the highest activity in sodium citrate at pH 5.0 and 55℃, and it specifically hydrolyzed p-nitrophenyl xylopyranoside and xylo-oligosaccharides (XOS). Also, BfdXYL2 could slowly hydrolyze AOS and AXOS such as A³X. Based on the detailed hydrolytic modes of action of three exo-hydrolases (BfdABF1, BfdABF3, and BfdXYL2) from Bf. dentium, their probable roles in the hemiceullose-utilization system of Bf. dentium are proposed in the present study. These intracellular exo-hydrolases can synergistically produce ʟ-arabinose and ᴅ-xylose from various AOS, XOS, and AXOS.

• 원예과학기술지
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• 제28권3호
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• pp.484-491
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• 2010

본 연구는 세대간 원예활동 프로그램이 노인과 유아의 정서와 자아존중감에 어떠한 영향을 미치는지 알아보고자 하였다. 세대간 원예활동 프로그램 실시 전, 후 노인의 정서를 비교한 결과, 대조군과 실험군 모두 유의한 차이를 나타내지 않았다. 노인의 자아존중감을 비교한 결과 대조군에서는 유의한 차이를 나타내지 않았지만, 실험군에서는 유의한 차이를 나타냈다($p$<0.01). 세대간 원예활동 프로그램 실시 전, 후 유아의 정서지능을 비교했을 때, 대조군은 유의한 차이를 나타내지 않았으나, 실험군은 유의한 차이를 나타냈다($p$<0.001). 유아의 자아존중감을 비교한 결과 대조군, 실험군 두 집단 모두 유의한 차이를 나타내지 않았다. 본 연구를 통해 세대간 원예활동 프로그램이 노인의 자아존중감과 유아의 정서지능 향상에 기여할 수 있었다.

• 인간식물환경학회지
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• 제22권4호
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• pp.355-364
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• 2019

This study was conducted to find out the effects of agro-healing activities and strolling on the physiological conditions of patients with chronic metabolic diseases. A total of 11 subjects participated in agro-healing activities, and their average age was 49.6±11.3. Changes in their salivary cortisol were compared and analyzed before and after participating in the agro-healing activity program, and it was found that the stress hormone decreased after participating in the program. Differences in brain wave between before and after participating in agro-healing activities and strolling were measured, and the ratio of alpha to high beta (RAHB) of subjects increased in most of the measured points after participating in agro-healing activities. In addition, the increase in the value in the temporal lobe (T3) that controls mortor skills and occipital lobe (O2) that controls visual functions indicates that the activities were effective in improving relaxation and stability. The relative low beta (RLB) power spectrum of subjects also statistically significantly decreased in the right occipital lobe (O2) that controls visual functions, the relative mid beta (RMB) and relative high beta (RHB) power spectrum decreased in the left temporal lobe (T3) and the right occipital lobe (O2). These results indicate that the activities were effective in improving relaxation under no stress and tension. The systolic and diastolic blood pressure of subjects after participating in agro-healing activities decreased from the prehypertension level to the normal level. These results indicate that horticultural activities and visual elements in healing farms, such as agro-healing activities and strolling, have positive impacts on patients with chronic metabolic diseases accompanied with diabetes or high blood pressure by increasing the brain activity and psycho-physiological conditions of participants.

• 생명과학회지
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• 제29권10호
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• pp.1062-1070
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• 2019

마늘(Allium sativum)의 주요 생리활성 성분들은 다양한 종류의 암에 대해 항암효과가 보고되고 있다. 본 연구에서는 활성추적분리방법(activity-guided purification)을 이용하여 마늘의 항암성분을 발굴하고자 하였다. 마늘 에탄올 추출물을 칼럼크로마토그래피로 얻은 각각의 분획물에 대해서 AGS세포의 증식 억제율을 검증하여 가장 효과가 좋은 분획물로부터 물질을 순수분리하여 구조를 동정한 결과 N-benzyl-N-methyldecan-1-amine (NBNMA)로 밝혀졌다. NBNMA의 암생장 억제효능을 검증하기 위해서 CT-26, AGS, HepG2, HCT-116, MCF7, B16F10 및 Sarcoma-180 세포에 대한 in vitro 효과와 CT-26 결장암 세포를 마우스에 이식한 다음 in vivo 효과를 조사하였다. NBNMA는 Bcl-2의 down-regulation과 Bad의 up-regulation을 유도하여 CT-26 세포의 세포사멸 촉진시켰다. 또한, NBNMA는 세포사멸의 외적 및 내적 경로에서 caspases 억제자인 caspase 3과 caspase 9의 활성을 약간 증가시켰다. CT-26세포를 이식한 쥐에 $19.13{\mu}M/kg$의 NBNMA를 21일 동안 경구투여한 결과 암종의 크기가 43% 감소하였다. NBNMA는 in vitro 및 in vivo에서 항암 효과를 나타내었는데, 이러한 결과는 마늘로부터 순수분리한 NBNMA가 대장암치료를 위한 항암제 후보물질로서 활용 가능성이 있을 것으로 기대된다.

• Journal of Microbiology and Biotechnology
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• 제28권9호
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• pp.1542-1546
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• 2018

Bacterial canker in kiwifruit is caused by Pseudomonas syringae pv. actinidiae (Psa). In this study, the bacteriophage PPPL-1 effective against Psa was characterized. Belonging to the Podoviridae family, PPPL-1 was effective against most Psa strains as well as most Pseudomonas syringae pathovars. PPPL-1 carries a 41,149-bp genome with 49 protein coding sequences and is homologous to the previously reported phiPSA2 bacteriophage. The lytic activity of PPPL-1 was stable up to $40^{\circ}C$, within a range of pH 3-11 and under 365 nm UV light. These results indicate that the bacteriophage PPPL-1 might be useful to control Psa in the kiwifruit field.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2007년도 춘계학술발표회
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• pp.211-212
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• 2007

• 인간식물환경학회지
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• 제24권6호
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• pp.573-584
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• 2021

Background and objective: This study was conducted with 10 men and women in their 50s-60s to investigate the effect of agro-healing activities on the improvement of mental health. Methods: The experimental group participated in total 8 sessions of agro-healing activities, once a week for 2 hours each, at a care farm in Wanju-gun. Physiological measurements were taken with an electroencephalogram (EEG), Salivettes samples, and blood pressure before and after the activities. Results: As a result of analyzing the changes in brainwaves of the experimental group before and after agro-healing activities, relative slow alpha (RSA), relative fast alpha (RFA), and ratio of alpha to high beta (RAHB), the indices of stability and relaxation, increased after the program with statistical significance. Also, the ratio of SMR to theta (RST) of the attention index increased on the right frontal lobes, temporal lobes, and left occipital lobes, and relative low beta (RLB) increasd on the frontal lobes, temporal lobes, and occipital lobes after the program with statistical significance. The sympathetic nervous system activity, which is a stress index, decreased after the program, whereas the parasympathetic nervous system activity, which is a relaxation index, increased, showing statistical significance (p < .05). As a result of analyzing the changes in blood pressure after the program, systolic blood pressure and diastolic blood pressure decreased from prehypertension to normal blood pressure, showing statistical significance. SThese results indicate that participating in agro-healing activities at a care farm for the 50-60s helps reduce stress and improve stability and relaxation as well as attention. Conclusion: Thus, developing and applying customized agro-healing programs for participants will have a positive effect on brain activity and psychophysiological improvement by relieving tension and stress. However, there are limitations in generalizing the results of this study since most of agro-healing farms have low accessibility that leads to a low level of participants.

• 인간식물환경학회지
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• 제23권3호
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• pp.267-276
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• 2020

Background and objective: Agro-healing is crucial with urban farming in the domestic. It is beyond the conventional agriculture. This study was carried out to assess the 2010s researches on domestic agro-healing and predict the future direction of agro-healing development. Methods: Among the articles published from 2010 to 2018, we collected some articles by searching keywords including agro-healing, garden activity, gardening, horticultural activity, horticultural program, horticultural therapy, plant effect, plant environment, plant growing program, plant impact, social gardening, urban agriculture and vegetable garden activity, selected 83 articles that were evaluated in advance, and analyzed by frequency analysis, t-test, and one-way ANOVA with SPSS 20.0. Results: Agro-healing journal articles were published the most in 2010, and have declined since then. In the classification according to the academic society, most of the journal articles were published by the Society for People, Plants, and Environment. The main targets of domestic agro-healing related to activities and programs were preschoolers, children and adolescents, accounting for 52.4% of the total. By the characteristics of the subjects, agro-healing programs and studies were conducted with special participants who needed special care compared to the general participants. The dependent variables were classified into six areas according to their attributes and the share of psychological and emotional areas was highest (42.6%) among them. In terms of the composition of the program, the share of those with 9-12 sessions was highest (36.7%) and the share of those with more than 20 participants was also highest (39.8%). Conclusion: It is recommended to operate agro-healing programs or industries focusing on the socially disadvantaged including those who have special needs or the underprivileged, but in order to create income for farms and expand the demand for agro-healing, it will be necessary to spread the perception that anyone without any physical or emotional issue can be the targets of and experience agro-healing. To meet the different needs of targets of agro-healing, it will be necessary to conduct objective and practical studies on broader areas and in the process the healing functions of agriculture and the strength of agro-healing needs to be further highlighted.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2011년도 추계학술발표회
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• pp.356-357
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• 2011