• Research in Plant Disease
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• v.20 no.3
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• pp.211-215
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• 2014

This research was performed to examine the protective activities of the mixtures of pine oil and copper hydroxide against bacterial spot and anthracnose on pepper plants. As for bacterial spot, the treatment of pine oil alone displayed high disease incidence (59.6%) and low protective effect (28.9%). In comparison, the treatments of mixtures and copper hydroxide alone showed protective activities of 66.8-76.1%. The mixture of pine oil and copper hydroxide (4:1) suppressed the most effectively bacterial spot on pepper. On the other hand, the mixture of pine oil and copper hydroxide (4:1) also showed the strongest protective effect against pepper anthracnose among the 4 treatments tested; its disease incidence and disease control value were 49.8% and 41.7%, respectively. The other treatments showed low protective activities with control values of 7.4-17.1%. These results suggested that the mixture of pine oil and copper hydroxide (4:1) can be used for the environmental-friendly disease control of bacterial spot and anthracnose on pepper.

• Journal of Nutrition and Health
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• v.53 no.3
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• pp.244-254
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• 2020

Purpose: Ixeris strigosa (IS) is a perennial plant that commonly grows in meadows. The leaves and roots of IS have been used in medicine as a sedative. This study evaluated the antioxidant and carbohydrate-digestive-enzyme inhibitory effects of IS to determine its potential as an essential antioxidant and glycemic inhibitor for type 2 diabetics. Methods: The antioxidative and α-amylase and α-glucosidase inhibitory activities were examined using the water extracts (ISW), ethanol extracts (ISE), and solvent fractions from IS. The antioxidative activities were measured using in vitro methods by measuring the 1,1-diphenyl-2-picrylhydrazyl and 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid radical scavenging activity. Results: Investigations of the total polyphenol, flavonoid content, in vitro antioxidant activity, and α-amylase and α-glucosidase inhibitory activities of the IS extract showed that the ISE had higher total phenolic and flavonoid contents than the ISW, as well as high antioxidant activity. The ethanolic extracts of IS (70%) had an α-amylase inhibitory activity of 78.55%. The ethyl acetate fraction (90.56%) showed higher α-glucosidase inhibitory activity than the positive control, acarbose (83.01%). Conclusion: Among the ISE fractions, the ethyl acetate and butanol fractions showed the best digestive enzyme inhibitory activity. Moreover, the antioxidant activity of the extract and the carbohydrate, α-amylase, and α-glucosidase inhibitory effects showed a stronger correlation with the total phenol and flavonoid contents compared to the ISW. As a result, the antioxidant and digestive enzyme inhibitory activities of high ISE are due to the phenolic compounds, particularly the flavonoid compounds. Therefore, ethyl acetate and butanol fractions of the 70% ethanol extract are excellent anti-diabetic functional materials.

• Journal of Mushroom
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• v.10 no.4
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• pp.203-207
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• 2012

The fruiting bodies of Ganoderma lucidum extracted by water and ethanol extraction. we are analyzed antioxidant effects and cancer cell growth inhibition rate. ASI 7004, 7014 was higher antioxidant effects than Trolox, BHA as control. Generally, the rest of strains was higher antioxidant effects than ABTs as control. Water extracts and ethanol extracts was treated to the Liver cancer cell(HepG2) and stomach cancer cell(AGS). Inhibition activities of Liver cancer cell(HepG2) is a high in D.W. extracts of ASI 7002, 7011, 7014, 7020. Inhibition activities of Liver cancer cell(HepG2) is a high in EtoH extracts of ASI 7011, 7019. Inhibition activities of Stomach cancer cell(AGS) is a high in D.W. extracts of ASI 7001, 7002, 7019, 7020. Inhibition activities of Stomach cancer cell(AGS) is a high in EtoH extracts of ASI 7001, 7002.

• Journal of Ginseng Research
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• v.37 no.4
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• pp.442-450
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• 2013

The aim of this study was to determine and compare the preventive effect of Korean White Ginseng and Red Ginseng on oxidative stress in $H_2O_2$-treated HepG2 cells. The roots of ginseng were extracted with 70% methanol and partitioned with butanol to obtain saponin fractions, which have been known as bioactive constituents of ginseng. 2',7'-Dichlorofluorescein diacetate (DCF-DA) assay and malondialdehyde (MDA) content were measured for evaluating intracellular reactive oxygen species (ROS) generation. Also, mRNA expressions and activities of antioxidant enzymes were analyzed to determine the antioxidant activity of saponin or non-saponin fractions of ginsengs. According to DCF-DA assay, $H_2O_2$-induced MDA release and ROS generation were significantly reduced by treatment with saponin fractions of white and red ginseng roots. Also, saponin fractions increased effectively intracellular antioxidant enzyme activities including catalase, glutathione peroxidase and superoxide dismutase in $H_2O_2$-treated HepG2 hepatoma cells. In general, red ginseng was more effective than white ginseng for reducing oxidative stress. These results indicate that administration of red ginseng may certainly contribute relatively stronger than white ginseng to prevent from damaging liver function by oxidative stress.

• Korean Journal of Adult Nursing
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• v.13 no.3
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• pp.409-419
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• 2001

The reference study was performed to investigate the nursing importance which was based a theoretical background related to horticultural therapy and to examine the possibility which horticultural therapy was applicable as a nursing intervention through analysis on a preceding study. The research subjects related to the areas of horticultural therapy which related as a nursing intervention, were psychological, physical, environmental, and psycho-social aspects. It is judged that the horticultural therapy is effective in treating depression, emotional disruption or anxiety. It seems to be also effective in increasing the muscular tension and, thereby, expanding the scope of joint movements. Such theories show that horticultural therapy may be a good alternative nursing means. Plants act to create a pleasant interior atmosphere by generating anion, controlling the temperature and humidity and purifying the air, and therefore, the horticultural therapy may be applied to clinic or environmental therapy. When horticultural therapy is used as a nursing intervention, patients' sociopsychological needs may be fulfilled. It has been found that horticultural therapy is instrumental in treating perceptive or emotional disruption, depression, loss of self-respect, disrupted everyday activities and social behaviors. In particular, horticultural therapy seems to be effective in managing chronic patients' crisis or improving life quality. Intervention method applied on a preceding study was activity therapy and scene therapy in the horticultural therapy The above findings suggest that the pro-environmental horticultural therapy is useful as a new paradigm of nursing or holistic nursing conducive to improvement of health. So, it is desirable to prove its effects by applying it in the clinic. In order to apply horticultural therapy as a nursing intervention, it will be necessary to educate nurses on principles and methods of horticultural therapy and encourage them to apply it in nursing and clinics. Moreover, it may well be necessary to develop landscape therapy as nursing intervention as well as a variety of horticultural therapy programs befitting the clinic conditions. On the other hand, we need to provide for some scientific ground for horticultural therapy through continued studies. In this regard, this study which focuses on patients' health improvement through change of environment. may well provide for a framework for such studies.

• Journal of People, Plants, and Environment
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• v.21 no.6
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• pp.533-543
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• 2018

This study aimed to examine how the horticultural therapy that was originated in the US has influenced South Korea and Japan and developed in the two countries as an interim check to promote qualitative growth of horticultural therapy with the growing social attention. It also aimed to look into the background of how horticultural therapy was introduced in Japan (which introduced horticultural therapy around the same time as Korea), and the process of the introduction, and compare them with those of Korea in order to set the direction for horticultural therapy. Data was collected to prepare the chronological table of horticultural therapy in Korea and Japan and investigate the flow. Interviews were conducted with the professors who first opened a horticultural therapy course in university so as to determine the introduction background. The analysis results are summarized as follows: Horticultural therapy was created to give mental comfort and emotional purification to those concerned with horticulture that had concerns over social phenomena. In Korea, there had been a process of finding a way of getting mental comfort and emotional purification in the economic slowdown in the late 1990s. Since 2001, there have been more attention and demand for horticultural therapy. As a result, there has been a tendency of studying general horticultural welfare activities and professional horticultural therapy separately. In Japan, the environmental pollution that has arisen since 1970s led to a concern over social orientation in the relation between plants and humans. The academic conference of global researchers to establish the horticultural therapy studies influenced the introduction of horticultural therapy in Korea and Japan. Both countries had no operations and system, and developed them independently. They had similar directions to seek, such as the department of horticultural therapy, need for professional education, active introduction of hospital practice (internship), and security of operating budget. Horticultural therapy has many competencies and thus requires constant research and expansion.

• Journal of People, Plants, and Environment
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• v.23 no.1
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• pp.55-65
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• 2020

This study conducted a survey on 163 parents in Korea to investigate their interest in plants, experience in growing plants, and perception on horticultural activity programs (HAP) for their children. Most children (80.4%) of the respondents had experience participating in HAPs, and most of their experience occurred during school hours in preschool educational institutions more than once a month (60.3%). The more interest in plants and experience in growing plants the parents had, the more experience the preschoolers had in participating in HAPs, which implies that the experience of the parents with plants affected their children's participation in HAPs. Parents' most preferred location of HAPs was preschool educational institutions, and most preferred time was school hours with a frequency of more than once a week (57.1%), which is more frequent than the current status of HAPs in preschool educational institutions. Parents also responded that the most preferred instructors of HAP has are those with a professional background in horticulture. Among the activities in HAP for preschoolers, parents most preferred cooking (M = 4.05) activity, and they did not dislike any of the activities. They responded that the facilities for HAP needed to be improved to make HAP more active in preschool educational institutions. Overall, based on the current results, developing suitable HAPs and facilities for preschoolers, fostering professional HAP instructors, and educating parents about horticulture would enhance HAPs in preschool educational institutions, thus providing preschoolers with many benefits of HAPs.

• Journal of Microbiology and Biotechnology
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• v.30 no.2
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• pp.155-162
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• 2020

Acetyl xylan esterase (AXE; E.C. 3.1.1.72) is one of the accessory enzymes for xylan degradation, which can remove the terminal acetate residues from xylan polymers. In this study, two genes encoding putative AXEs (LaAXE and BhAXE) were cloned from Lactobacillus antri DSM 16041 and Bacillus halodurans C-125, and constitutively expressed in Escherichia coli. They possess considerable activities towards various substrates such as p-nitrophenyl acetate, 4-methylumbelliferyl acetate, glucose pentaacetate, and 7-amino cephalosporanic acid. LaAXE and BhAXE showed the highest activities at pH 7.0 and 8.0 at 50℃, respectively. These enzymes are AXE members of carbohydrate esterase (CE) family 7 with the cephalosporine-C deacetylase activity for the production of antibiotics precursors. The simultaneous treatment of LaAXE with Thermotoga neapolitana β-xylanase showed 1.44-fold higher synergistic degradation of beechwood xylan than the single treatment of xylanase, whereas BhAXE showed no significant synergism. It was suggested that LaAXE can deacetylate beechwood xylan and enhance the successive accessibility of xylanase towards the resulting substrates. The novel LaAXE originated from a lactic acid bacterium will be utilized for the enzymatic production of D-xylose and xylooligosaccharides.

• Korean Journal of Medicinal Crop Science
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• v.17 no.1
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• pp.1-7
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• 2009

Several parts of Lythrum salicaria were used for this study. Scavenging activities on radicals, inhibitory activity on linoleic acid peroxidation and total phenol contents of extracts from root, flower, and aerial part were evaluated. Flower and root selected from in vitro assay were subjected to in vivo assay on $CCL_4$-induced liver injury rat model for two weeks. Carbon tetrachloride intoxication on rats produced large amounts of hepatic lipid peroxidation product, thiobarbituric acid reactive substance (TBARS) compared with normal rats. Treatment with root extract of L. salicaria (LSR) showed effective inhibitory activity on lipid peroxidation product. Administration with LSR extract significantly alleviated $CCL_4$-induced increase in GPT activity which were more effective than silymarin. The results of this study suggest that root and flower of L. salicaria have antioxidant and liver protecting activities, and root part is the most effective candidate to develop a new functional material.

• Research in Plant Disease
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• v.12 no.1
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• pp.40-45
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• 2006

The plant defence activator, Acibenzolar-S-methyl [benzo (1,2,3) thiadiazole-7-carbothioic acid-S-methyl ester, ASM] was assayed on tomato seedlings for its ability to induce resistance against Botrytis cinerea, the causal agent of gray mold in tomato. Pre-treatment of plants with ASM reduced the severity of the disease as well as the growth of the mycelium in plants. In ASM treated plants, reduction in disease severity (up to 55%) was correlated with suppression of mycelia growth (up to 46.5%) during the time course of infection. In plants treated with ASM, activities of peroxidase were determined as markers of resistance. Applications of ASM induced Progressive and significant increase of the enzyme in locally treated tissues. Such responses were expressed earlier and with a much higher magnitude when ASM-treated seedlings were challenged with the pathogen, thus providing support to the concept that a signal produced by the pathogen is essential for triggering enhanced synthesis and accumulation of the enzymes. No such activities were observed in water-treated control plants. Therefore, the slower symptom development and reduction in mycelium growth in ASM treated plants might be due to the increase in activity of oxidative and antioxidative protection systems in plants.