• Title/Summary/Keyword: Hormonse

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Effects of Hormones, Oviduct Epithelial Cells, Cumulus Cells during the In Vitro Culture in Medium on In Vitro Developmental Rates of Bisected Bovine Embryos (소 분할배와 호르몬, 난관상피세포, 난구세포와의 공배양이 체외발생율에 미치는 영향에 관한 연구)

  • 김상근;남윤이;이명헌;이만휘
    • Korean Journal of Animal Reproduction
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    • v.21 no.3
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    • pp.281-285
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    • 1997

  • The study was conducted to investigate on in vitro developmental rates of bisected bovine embryos co-culture in 10% FCS＋TCM-199 media containing hormones, oviductal epithelial cells and cumulus cells 0 to 7 days after bisection. In vitro developmental rates was defined as development rates on in vitro culture or FDA-test. The results are summarized as follows : 1. In vitro developmental rates of bisected bovine embryos co-cultured in 10% FCS＋TCM-199 media containing PMSG＋hCG, PMSG＋$\beta$-estradiol, hCG＋$\beta$-estradiol, PMSG, hCG 0 to 3 days and 4 to 7 days were 16.7~30.0% and 11.1~25.0%, respectively. In vitro developmental rates of bisected embryos co-cultured in 10% FCS＋TCM-199 media containing hormones significantly higher than that of non co-culture. 2. In vitro developmental rates of bisected bovine embryos co-cultured 10% FCS＋TCM-199 media containing oviductal epithelial cells 0 to 3 days and 4 to 7 days were 25.0% and 22.2%, respectively.

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