• Clinical and Experimental Reproductive Medicine
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• 제3권2호
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• pp.29-34
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• 1976

동물에서 정상적인 임신유지를 함에 필요한 홀몬의 양을 측정하기 위하여 토끼에서 인공수정을 실시한 다음날 양쪽 ovary를 제거하고, 홀몬대치요법(replace therapy)에 의해서 인공적으로 임신상태를 분만시까지 유지시키면서 competitive protein-binding assay method을 이용하여 혈중 progestin의 농도를 측정하여 정상 임신토끼와 비교하였던 바 다음과 같은 결과를 얻었다. 1) 정상임신토끼에 있어서 혈중 progestin의 평균 농도 변화는 제 1일에 $1.7{\sim}2.7$ ng/ml으로 시작해서 차차 증가되어 제$13{\sim}15$일까지는 $19.8{\sim}25.3$ ng/ml까지 상승하였다가 그후 분만시까지 서서히 감소되었다. 2) 홀몬 대치요법에 의해서 인공적으로 임신을 유지시킨 토끼의 혈중 progestin 농도는 정상토끼의 progestin 농도의 변화와 매우 비슷한 변화곡선을 나타내었다.

• Preventive Nutrition and Food Science
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• 제9권4호
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• pp.336-341
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• 2004

Leptin, resisitn and PAI-1 (plasminogen activator inhibitor-1) are synthesized and secreted by rodent fat cells and recently postulated to be an important link to obesity. This study was conducted to characterize the hormonal regulation of leptin, resistin, and PAI-1 gene expression in the 3T3-L1 adipocytes. The cells were treated with 0.5 $\mu$M insulin, 1 $\mu$M dexamethasone (Dex), or 0.05 $\mu$M triiodothyronine (T3) for 72 hours. The mRNA levels of each peptide were measured by semi-quantitative RT-PCR. The mRNA level of the leptin-producing ob gene was significantly increased by insulin, Dex, and T3 by 3.2-, 3.1- and 2.7-fold, respectively, compared to the control (p < 0.05). The level of resistin mRNA was increased by insulin, Dex, and T3 by 2.7-, 2.5- and 2-fold, respectively, compared to the control (p < 0.05). Likewise, the level of PAI-1 mRNA was significantly increased by insulin, Dex, and T3 compared to the control (p < 0.05). Taken together, our results suggest that insulin, Dex, and T3 may regulate the gene expression of leptin, resistin, and PAI-1 in 3T3-L1 adipocytes.

• 수면정신생리
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• 제5권1호
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• pp.12-17
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• 1998

Circadian rhythms in subjective alertness, mood, and performance can be classified as psychological rhythm, compared with physiological rhythm such as body temperature and hormonal change. While in normal condition entrained by 24hr zeitgeber, subjective alertness would reach its maximum value around midday, subjective alertness would parallel body temperature rhythm with its peak at evening in non-entrained, free-running state. With desynchronization technique, subjective alertness rhythm is thought to be controlled by both temperature and sleep-wake rhythm oscillator. Circadian performance rhythms depend on the kind of task tested. It shows parallelism with body temperature rhythm when subjects are tested with simple, repetitive task. But when tested with tasks requiring complex verbal reasoning or immediate memory, subjects would perform them best at early morning, with performance decreasing as time of day advances. The desynchronization technique shows that circadian performance rhythm of simple, repetitive task is dependent on temperature oscillator but circadian performance rhythm of complex verbal reasoning is influenced by both temperature and sleep-wake rhythm oscillator or another independent oscillator. It would be worthwhile to compare psychological rhythm with hormonal change such as cortisol and melatonin. And more simple and time-saving method than desynchronization technique may facilitate the study of the mechanism underlying psychological rhythm.

• 한국독성학회:학술대회논문집
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• 한국독성학회 2003년도 추계학술대회
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• pp.149-149
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• 2003

It is well known that many pesticides possess hormonal activity, and thus have been classified as endocrine disruptors. Currently, pyrethroid insecticides are in worldwide use to control in door pests, providing potential for environmental exposure. A few studies of hormonal activities of these pyrethroid insecticides, however, have been reported, and are controversial between studies.(omitted)

• 한국독성학회:학술대회논문집
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• 한국독성학회 2002년도 Molecular and Cellular Response to Toxic Substances
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• pp.167-167
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• 2002

Many pesticides possess hormonal activity and have been thus classified as endocrine disruptors. Permethrin, a pyrethroid insecticide has little been characterized to it's hormonal potential. In the present study, effects of neonatal exposure to permethrin on the sex steroid receptor levels and hoxa-10 expression of the uteri were investigated in female mice.(omitted)

• Biomolecules & Therapeutics
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• 제22권6호
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• pp.570-576
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• 2014

Studies have demonstrated that electromagnetic waves, as the one of the most important physical factors, may alter cognitive and non-cognitive behaviors, depending on the frequency and energy. Moreover, non-ionizing radiation of low energy waves e.g. very low frequency waves could alter this phenomenon via alterations in neurotransmitters and neurohormones. In this study, short, medium, and long-term exposure to the extremely low frequency electromagnetic field (ELF-EMF) (1 and 5 Hz radiation) on behavioral, hormonal, and metabolic changes in male Wistar rats (250 g) were studied. In addition, changes in plasma concentrations for two main stress hormones, noradrenaline and adrenocorticotropic hormone (ACTH) were evaluated. ELF-EMF exposure did not alter body weight, and food and water intake. Plasma glucose level was increased and decreased in the groups which exposed to the 5 and 1Hz wave, respectively. Plasma ACTH concentration increased in both using frequencies, whereas noradrenaline concentration showed overall reduction. At last, numbers of rearing, sniffing, locomotor activity was increased in group receiving 5 Hz wave over the time. In conclusions, these data showed that the effects of 1 and 5 Hz on the hormonal, metabolic and stress-like behaviors may be different. Moreover, the influence of waves on stress system is depending on time of exposure.

• 한국수정란이식학회지
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• 제33권4호
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• pp.271-280
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• 2018

Here, we evaluated the mode of programmed cell death during porcine oocyte maturation by comparing the two major pathways associated with programmed cell death, apoptosis (type I), and autophagy (type II). We investigated the expression and localization of major genes involved in autophagy and apoptosis at mRNA and protein levels. Furthermore, the effect of hormonal stimulation on autophagy and apoptosis was analyzed. We found that the activity of autophagy-associated genes was increased in the cumulus-oocyte complexes (COCs) following follicle-stimulating hormone (FSH) treatment, while the addition of luteinizing hormone (LH) reversed this effect. The expression of proteins associated with autophagy was the highest in FSH-treated COCs. On the other hand, caspase-3 protein level was maximum in COCs cultured with LH. The treatment with rapamycin resulted in the effect similar to that observed with FSH treatment and increased autophagy activity. Thus, hormonal stimulation of pig oocytes resulted in distinct patterns of maturation. The high-quality oocytes majorly relied on the type II pathway (autophagy), while the type I pathway (apoptosis) was more prominent among poor-quality oocytes. Further investigation of this distinction may allow the development of techniques to produce high-quality oocytes in porcine in vitro fertilization.

• Brain Tumor Research and Treatment
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• 제6권2호
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• pp.82-85
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• 2018

Extremely massive sellar xanthogranuloma (XG) are rare, and the surgical outcome and prognosis are not well known. XG remain unknown whether they are derived from Rathke's cleft cysts (RCCs) or craniopharyngiomas (CPs) following extensive inflammation and metaplasia, to the point that no epithelium is readily identifiable. These lesions usually tend to occur in younger patients (mean 28.3 years), have a smaller diameter, and remain primarily intrasellar region with infrequent calcification. This 36-year-old man presented our hospital with visual deterioration. At the time of visit, there were no neurological problems other than visual field defect and hormonal disorder. He visited our hospital in 2007 due to headache and decreased vision, and underwent transphenoid surgery for pituitary RCC. Since then, he has received treatment at our hospital for postoperative hormonal disorders. Through preoperative imaging study, the author suspected CP and underwent surgery. During the operation, the adhesion of the tumor to the surrounding major neurovascular structures was severe in the naked eyes, but the tumor could be removed more easily than expected. The postoperative histological findings were confirmed as XG. The postoperative course was uneventful. Compared to the previous literature, this case is a case where the size of XG is very large in a sellar region and it can be proved that it originated from the RCC. And regular follow-up is necessary to confirm the prognosis after surgery.

• 대한정형도수물리치료학회지
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• 제27권2호
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• pp.87-92
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• 2021

Purpose: This study aimed to investigate the effects of myofascial release technique on pain threshold and hormonal changes in patients with adhesive capsulitis of the shoulder. Methods: Eight patients with adhesive capsulitis were treated with the myofascial release technique. Myofascial release is a form of manual therapy that involves the application of a low load, long duration stretch to the myofascial complex, intended to restore optimal length, decrease pain, and improve function. Blood tests and pressure pain threshold (PPT) examinations were performed on their first visit. On their second visit, the myofascial release technique was applied to the shoulder for 20 min. Then, blood tests and PPT were re-evaluated to determine the effects of the myofascial release technique on pain threshold and hormonal changes. Results: Pain threshold increased from 2.92 to 24.13 lb after treatment. Epinephrine decreased from .13 to .08 ng/mL whereas norepinephrine increased from .25 to .41ng/㎖ after treatment. Conclusion: Myofascial release technique in patients with adhesive capsulitis increased pain thresholds, norepinephrine and decreased epinephrine levels.

• 한국동물생명공학회지
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• 제37권1호
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• pp.42-47
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• 2022

This study aimed to determine whether hormonal hypersecretion could cause morphological problems in the mouse vagina and affect the ovaries and nearby extra uterine organs. All mice were synchronized to estrus before the experiment. Then human chorionic gonadotropin (hCG), progesterone, and testosterone were continuously administered for about 6 days to maintain hormone hypersecretion, and then morphological changes were analyzed, and Matrix metalloproteinases (MMPs) activity and Casp-3 expression were evaluated. As a result of the analysis, in the case of hCG, the morphological change did not show a significant difference from the vagina of normal estrus. In the case of progesterone, changes were observed in the mucosa zone and basal membrane, and it was confirmed that the activity of MMPs was increased in squamous epithelium cells. On the other hand, in the case of testosterone, overall changes in vaginal tissues were observed, and MMPs activity was increased to a very high level in all sections. The expression of Casp-3 was also the highest compared to other groups. Therefore, as a result of this study, it is thought that hormone hypersecretion affects the morphological changes of the vagina other than the ovaries and uterus and induces the activity of MMPs to cause morphological degeneration of tissues.