• International Journal of Stem Cells
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• v.16 no.4
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• pp.438-447
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• 2023

Recently, ex-vivo gene therapy has emerged as a promising approach to enhance the therapeutic potential of mesenchymal stem cells (MSCs) by introducing functional genes in vitro. Here, we explored the need of using selection markers to increase the gene delivery efficiency and evaluated the potential risks associated with their use in the manufacturing process. We used MSCs/CD that carry the cytosine deaminase gene (CD) as a therapeutic gene and a puromycin resistance gene (PuroR) as a selection marker. We evaluated the correlation between the therapeutic efficacy and the purity of therapeutic MSCs/CD by examining their anti-cancer effect on co-cultured U87/GFP cells. To simulate in vivo horizontal transfer of the PuroR gene in vivo, we generated a puromycin-resistant E. coli (E. coli/PuroR) by introducing the PuroR gene and assessed its responsiveness to various antibiotics. We found that the anti-cancer effect of MSCs/CD was directly proportional to their purity, suggesting the crucial role of the PuroR gene in eliminating impure unmodified MSCs and enhancing the purity of MSCs/CD during the manufacturing process. Additionally, we found that clinically available antibiotics were effective in inhibiting the growth of hypothetical microorganism, E. coli/PuroR. In summary, our study highlights the potential benefits of using the PuroR gene as a selection marker to enhance the purity and efficacy of therapeutic cells in MSC-based gene therapy. Furthermore, our study suggests that the potential risk of horizontal transfer of antibiotics resistance genes in vivo can be effectively managed by clinically available antibiotics.

• The korean journal of orthodontics
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• v.23 no.4 s.43
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• pp.619-631
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• 1993

Tooth movement by segment is one of the means which are frequently used in daily orthodontic practice. When we retract or intrude a tooth or teeth, we should recognize the center of resistance of the certain tooth or teeth. There have been many studies about the center of resistance of a single tooth, not so much was about the tooth-segment. At the present study the center of resistance of the maxillary anterior segment is experimentally investigated by using laser reflection technique and metal splints on the human dry skull. The variables of intrusive force magnitude are divided into two groups, 50g and 100g groups. The results were as follows ; 1. The center of resistance of the maxillary anterior segment composed of the central and lateral incisors was at the mesial portion of canine crown at the coronal level. 2. The center of resistance of the maxillary anterior segment composed of the central and lateral incisors and canines is between the canine and the 1st premolar crowns at the coronal level.

• Earthquakes and Structures
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• v.24 no.3
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• pp.205-215
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• 2023

Currently, many studies are underway at home and abroad on the seismic performance evaluation and dry construction method of the masonry structure. In this study, a dry stack masonry wall system without mortar using concrete blocks is proposed, and investigate the seismic performance of dry filling wall frames through experimental studies. First, two types of standard blocks and key blocks were designed to assemble dry walls of concrete blocks. And then, three types of experiments were manufactured, including pure frame, 1/2 height filling wall frame, and full height filling wall frame, and cyclic load experiments in horizontal direction were performed to analyze crack patterns, load displacement history, rebar deformation yield, effective stiffness change, displacement ductility, and energy dissipation capacity. According to the experimental results, the full height filling wall frame had the largest horizontal resistance against the earthquake load and showed a high energy dissipation capacity. However, the 1/2 height filling wall frame requires attention because the filling wall constrains the effective span of the column, limiting the horizontal displacement of the frame. In addition, the concrete block was firmly assembled in the vertical direction of the wall as the horizontal movement between the concrete blocks was allowed within installation margin, and there was no dropping of the assembled concrete block.

• Journal of the Korean GEO-environmental Society
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• v.21 no.7
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• pp.5-16
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• 2020

Currently, the seismic design standards have been strengthened due to the occurrence of the Gyeongju and Pohang earthquake, and seismic performance evaluation of existing facilities is being conducted. It aims to secure a seismic performance effect during earthquakes by improving the micro-pile method, which can be constructed in limited confined places while minimizing damage to existing facilities. The improvement method is to construct all the piles in the square-tray-type plate on the top of the pile by constructing the slope pile in the form of an umbrella around the vertical pile, the main pillar. In this paper, the numerical analysis was performed to analyze the horizontal displacement behavior of an umbrella-type micropile for various real-measurement seismic waves in sandy soil. As a result of numerical analysis, the softer the ground, the better the effect of horizontal resistance of umbrella-type micropile. The horizontal displacement reduction effect was pronounced when the embedded depth was 15 m or more at the same ground strength, and it was found to be effective in earthquakes if it was settled on the ground with an N value of 30 or more. The embedded depth and horizontal displacement suppression effect of the micropile was proportional. Generally, the weaker the ground, the greater the displacement suppression effect. Umbrella-type micropile had a composite resistance effect in which the vertical pile resists the moment and inclined pile resists the axial force.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.35 no.3
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• pp.335-342
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• 1999

The purpose of present research is to develop and efficient numerical method for the calculation of potential flow and predict the wave-making resistance for the application to ship design of tuna purse seiner. Havelock was considered the wave resistance of a post extending vertically downwards through the water from the surface, its section by a horizontal plane being the same at all depths and having its breadth small compared with its length. This enables us to elucidate certain points of interest in ship resistance. However, the ship has not infinite draft. So, the problem which is investigated ind detail in this paper is the wave resistance of a mathematical quadratic model in a uniform stream. The paper deals with the numerical calculation of potential flow around the series 60 with forward velocity by the new slender ship theory. This new slender ship theory is based on the asymptotic expression of the Kelvin-source, distributed over the small matrix at each transverse section so as to satisfy the approximate hull boundary condition due to the assumption of slender body. The numerical results using the panel shift method and finite difference method are compared with the experimental results for wigley mono hull. There are no differences in the wave resistance. However, it costs much time to compute not only wave resistance but also wave pattern over some range of Froude numbers. More improvements are strongly desired in the numerical procedure.

• Fire Science and Engineering
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• v.25 no.5
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• pp.76-84
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• 2011

Steel beams are one of primary member and those carries the horizontal load and floor load to axial member. To avoid structural failure when the steel beams are exposed to fire, fire resistance performance requires. Till now, the evaluation for fire resistance of the beam was conducted using the maximum load and standard fire curve defined in the KS F 2257. But recently the constructional patterns are changing toward multi-function performance to get a better structural performance and fire resistance as well. In this paper to get the databases for fire resistance, limiting temperatures of the beam, load-bearing fire tests according to load ratios, two grades of compressive concrete strengths were applied.

• Magazine of the Korean Society of Agricultural Engineers
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• v.42 no.4
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• pp.115-123
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• 2000

This study compared the degree of consolidation by hyperbolic, curve fitting , Asaoka's and methods using values measured with a theoretical curve in consideration of smear effect and well resistance. The degree of consolidation by the Hyperboilc method was underestimated than the degree of consolidation by Curve fitting. Asaoka's , and Monden's methods. The typical range of the coefficient of horizontal consolidation was Ch=(2-3)Cv in the case considering smear effect and well resistance, and Ch =(0.5-2.1) Cv in the case disregarding smear effect and well resistance. The degree of consolidation obtained by ground settlement monitoring was nearly the same value when the coefficient of smear zone permeability by back analysis was shown to be half that of in-situ and the diameter of the smear zone was shown to be double that of mandrel. By increasing the diameter reduction ratio of the drain, the time of consolidation was delayed. The effect of well resistance showed that the case of a small coefficient of permeability was much more than in the case of a large coefficient of permeability . It was recommended that when designing diameter reduction of a drain, well resistance should be considered.

• Journal of Mechanical Science and Technology
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• v.19 no.6
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• pp.1338-1346
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• 2005

Effects of thermal contact resistance between heater and susceptor, susceptor and graphite board in a MOCVD reactor on temperature distribution and film growth rate were analyzed. One-dimensional thermal resistance model considering thermal contact resistance and heat transfer area was made up at first to find the temperature drop at the surface of graphite board. This one-dimensional model predicted the temperature drop of 18K at the board surface. Temperature distribution of a reactor wall from the three-dimensional computational fluid dynamics analysis including the gap at the wafer position showed the temperature drop of 20K. Film growth rates of InP and GaAs were predicted using computational fluid dynamics technique with chemical reaction model. Temperature distribution from the three-dimensional heat transfer calculation was used as a thermal boundary condition to the film growth rate simulations. Temperature drop due to the thermal contact resistance affected to the GaAs film growth a little but not to the InP film growth.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.6
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• pp.267-274
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• 2021

Among several methods for determining the allowable lateral resistances of piles, the subgrade reaction method and ultimate lateral resistance method are generally used. To determine the effects of the soil conditions, pile head restraint conditions, and pile lengths on determining the allowable lateral resistances of piles, computations of the allowable lateral resistances of piles using the two methods were executed, and the computation results were compared. For piles in soft cohesive soil, the pile design is governed by the allowable lateral resistance of a pile from subgrade soil reaction method regardless of the pile head restraints conditions and pile lengths. The allowable lateral resistance of a pile from the ultimate lateral resistance governs the design as the undrained shear strength increases. Except for the case of a short pile, which is installed in loose granular soil, the allowable lateral resistance of a pile from ultimate lateral resistance governs the design of laterally loaded piles. According to this study, computation of the ultimate lateral resistance of a pile is needed, even though some opinions suggest that the design of a laterally loaded pile is satisfied only by the subgrade reaction method. The pile width barely influences the coefficient of horizontal subgrade reaction. Realistically, the effect of the pile width can be disregarded in the condition of common pile widths of 20~90cm.

• The KSFM Journal of Fluid Machinery
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• v.19 no.3
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• pp.43-47
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• 2016

Pile foundations constructed on extremely cold regions cause serviceability problems of superstructures from repeated actions of ground freezing and thawing. Oil sand module plants are mainly constructed on seasonal frozen ground. Due to the freezing and thawing actions of grounds, vertical movements of piles have been observed. To solve these erratic pile movement problems, thin vertical layer of PET aggregates is installed around the pile shaft to prevent potential unfavorable pile movements. There is no known method to calculate "thin PET aggregate layer" -surrounded pile shaft resistance (capacity) against vertical loads; therefore, this experimental research is conducted. Specifically, in this study, horizontal (normal) pressures on pile shaft were assessed varying PET aggregate layer thickness based on the experiment.