• Title/Summary/Keyword: Homoorientin

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Enhanced effect extraction of Antioxidant substance Homoorientin from Pseudosasa japonica and Phyllostachys bambusoides leaves using Ultrasonic wave system (초음파를 이용한 이대와 왕대 잎으로부터 항산화물질 Homoorientin의 추출효율 향상)

  • 이광진;신용국;김영식
    • KSBB Journal
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    • v.24 no.2
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    • pp.189-194
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    • 2009
  • In this work, antioxidant substance homoorientin from Pseudosasa japonica and Phyllostachys bambusoides leaves wereextracted using ultrasonic system, and analyzed by reversed-phase high performance liquid chromatography (RP-HPLC) on-line ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) antioxidant screening method. Also, the various experimental variables were the frequency and time of ultrasonic system. From the results, homoorientin was high extracted at the experimental condition of low frequency 35 kHz and time 60 min. And the content of homoorientin in Phyllostachys bambusoides was remarkably higher than that in the Pseudosasa japonica extract.

Rapid Identification of Homoorientin from Phyllostachys bambusoides Leaves by HPLC On-line $ABTS^+$ Screening Method (HPLC On-line $ABTS^+$ Screening을 이용한 왕대(Phyllostachys bambusoides) 잎으로부터 Homoorientin의 확인)

  • Lee, Kwang-Jin;Liang, Chun;Yang, Hye-Jin;Ma, Jin-Yeul
    • YAKHAK HOEJI
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    • v.56 no.4
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    • pp.217-221
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    • 2012
  • To determination of antioxidant substance homoorientin, from Phyllostachys bambusoides leaves, the ultrasonic extraction and HPLC on-line $ABTS^+$ screening method were empolyed. Also, the various experimental variables such as the frequency and time of ultrasonic system were investigated and homoorientin was extracted efficiently at the low frequency 35 kHz and the extraction time 60 min. The values were positive peak 1574.71 (relative area, 23.67%) and negative peak 6924.34 (relative area, 1.23%), respectively. This HPLC on-line $ABTS^+$ screening method was rapid and efficient to search for antioxidants from natural products. These results will provide a database for investigating the constituents of natural products and the resources of pharmaceutical and cosmetic products.

Enhanced extraction and Antioxidant activity analysis of Flavone C-glycosides from Black bamboo using Ultrasonic wave (초음파를 이용한 오죽으로부터 Flavone C-glycosioes의 추출 및 항산화활성분석 향상)

  • Choi, Sun-Do;Lee, Kwang-Jin
    • KSBB Journal
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    • v.23 no.4
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    • pp.297-302
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    • 2008
  • In this work, the amounts of Flavone C-glycosides homoorientin, orientin extracted from Black bamboo by various ultrasonic waves frequency (35, 72, 170 KHz, 300 Watt $\pm$1) time (15, 30, 60 min) and temperature ($25^{\circ}C$) were compared using 50% aqueous ethanol solution. And describesanalysis of the antioxidant potential of Black bamboo using an high-performance liquid chromatography (HPLC) on-line $ABTS^+$antioxidant screening method. In conjunction with the analysis of their 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid ($ABTS^+$) radical scavenging ability. The optimum operating conditions were experimentally determined to analyze the Flavone C-glycosides homoorientin, orientin in the pretreatment ultrasonic extracts. From the results, the effect on extraction yield of variations in frequency, solvent composition and extraction time was investigated. The highest yield of Black bamboo was obtained by ultrasonic waves with a frequency of 72 KHz and an extraction time of 60 min, The extraction frequency 35 KHz ($25^{\circ}C$), time 30 min was selected as an optimal antioxidant activity condition.