• Asian Pacific Journal of Cancer Prevention
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• 제14권5호
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• pp.3197-3203
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• 2013

Background: microRNAs (miRNAs) that regulate proliferation, invasion and metastasis are considered to be the principal molecular basis of tumor heterogeneity. Breast cancer is not a homogeneous tissue. Thus, it is very important to perform microarray-based miRNA screening of tumors at different sites. Methods: Breast tissue samples from the centers and edges of tumors of 30 patients were classified into 5 clinicopathological subtypes. In each group, 6 specimens were examined by microRNA array. All differential miRNAs were analyzed between the edges and centers of the tumors. Results: Seventeen kinds of miRNAs were heterogeneously distributed in the tumors from different clinicopathological subtypes that included 1 kind of miRNA in Luminal A and Luminal B Her2+ subtypes, 4 kinds in Luminal A and Her2 overexpression subtypes, 6 kinds in Luminal B Ki67+ and Luminal B Her2+ subtypes, 2 kinds between Luminal B Ki67+ and triple-negative breast cancer (TNBC) subtypes, 2 kinds between Luminal B Her2+ and TNBC subtypes, and 2 kinds between Luminal B Ki67+, Luminal B Her2+, and TNBC subtypes. Twenty kinds of miRNAs were homogenously distributed in the tumors from different clinicopathological subtypes that included 6 kinds of miRNAs in Luminal B Ki67+ and Luminal B Her2+ subtypes, 1 kind in Luminal B Ki67+ and Her2 overexpression subtypes, 10 kinds between Luminal B Ki67+ and TNBC subtypes, 2 kinds in Luminal B Her2+ and TNBC subtypes, and 1 kind between Luminal B Ki67+, Luminal B Her2+, and TNBC subtypes. Conclusions: A total of 37 miRNAs were significantly distributed in tumors from the centers to edges, and in all clinicopathological subtypes.

• 정보관리학회지
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• 제25권3호
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• pp.273-296
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• 2008

본 연구는 선행연구의 연구결과를 데이터로 간주하여 통계분석하는 메타분석방법을 사용하여 사서의 직무만족에 영향을 미치는 요인을 연구하였다. 선행연구 27편으로부터 집단변수로는 남녀성별과 결혼유무, 종속변수로는 전체직무만족도와 7개 하위변수(직무자체, 급여, 승진, 감독, 근무환경, 사회적 인식, 자아실현)가 선정되어 분석되었다. 분석결과 남녀간 사회적 인식과, 승진, 감독, 근무환경의 효과크기는 유의한 차이를 보였으나, 감독과 근무환경의 효과크기만 동질적인 것으로 분석되었다. 이질적으로 분석된 사회적 인식과 승진에 대해서는 랜덤효과분석을 추가로 수행한 결과 승진의 효과크기만 유의한 차이를 보였다. 결혼여부에서는 직무자체와 급여, 자아실현의 효과크기가 유의한 차이를 보였으나, 이질적으로 판명된 직무자체의 효과크기는 랜덤효과분석에서 유의한 차이를 보이지 못하였다. 연구시기 (2000년전후)는 중재변수가 되지 못한 것으로 분석되었다.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2011년도 제41회 하계 정기 학술대회 초록집
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• pp.199-199
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• 2011

The addition of a carbanion to ${\yen}{\acute{a}}{\yen}{\hat{a}}$-unsaturated carbonyl compounds is of importance in the C-C bond formation reactions for modern pharmaceuticals and organic synthesis. Recently, heterogeneous asymmetric catalysis became more attractive area of research because of the easy recovery and separation of the catalyst from the reaction system. Most of synthetic methods for heterogeneous catalysts were grafting or immobilization of homogeneous catalyst onto the solid supports. Trans-1,2-Diaminocyclohexane(DACH) and L-proline ligands have been enormously used as chiral ligands in several catalytic transformation under homogenous conditions. Our group prepared l-proline functionalized mesoporous silica was synthesized under acidic condition using a poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) triblock copolymer template (EO20PO70EO20, Pluronic P-123, BASF). Furthermore, we successfully directly synthesized trans-1,2 diaminocyclohexane functionalized mesoporous silica by using microwave method. The direct functionalization of chiral ligand into the framework of mesoporous materials is expected to be useful for the heterogeneous asymmetric catalysis. So, we adopt the direct synthesis of chiral ligand functionalized mesoporous silica by using thermal and microwave irradiation. Then, chiral ligand functionalized mesoporous silicas were applied to enantioselective asymmetric catalytic reactions.

• Reproductive and Developmental Biology
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• 제33권4호
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• pp.263-271
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• 2009

Cardiovascular diseases (CVDs) are one of the most cause of death around the world and fields of interest for cardiac stem cells. Also, current use of terminally differentiated adult cardiomyocytes for CVDs has limited regenerative capacity therefore any significant cell loss may result in the development of progressive heart failure. Human embryonic stem cells (hESCs) derived from blastocyst-stage embryos spontaneously have ability to differentiate via embryo-like aggregates (endoderm, ectoderm and mesoderm) in vitro into various cell types including cardiomyocyte. However, most effective molecule or optimized condition which can induce cardiac differentiation of hESCs is rarely studied. In this study, we developed both spontaneous and inductive cardiomyocyte-like cells differentiation from hESCs by treatment of induced-factors, 5-azacytidine, BMP-4 and cardiogenol C. On the one hand, spontaneous and inductive cardiomyocyte-like cells showed that cardiac markers are expressed for further analysis by RT-PCR and immunocytochemistry. Interestingly, BMP-4 greatly improved homogeneous population of the cardiomyocyte-like cells from hESCs CHA15 and H09. In conclusion, we verified that spontaneously differentiated cells showed cardiac specific markers which characterize cardiac cells, treated extrinsic factors can manage cellular signals and found that hESCs can undergo differentiation into cardiomyocytes better than spontaneous group. This finding offers an insight into the inductive factor of differentiated cardiomyocytes and provides some helpful information that may offer the potential of cardiomyocytes derived from hESCs using extrinsic factors.

• 공업화학
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• 제27권6호
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• pp.664-668
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• 2016

무단 폐기물 투기로 인해 토양, 지하수, 하천 등 환경오염이 심각해지고 있다. 본 연구에서는 J시에서 발생된 무단 투기 유류를 분석함으로써 어떤 유종인지를 분석하였다. 물, 고형불순물, 유류 혼합물 형태를 전처리하여 균질한 유류성분을 얻은 뒤, 물성분석, 원소분석, 및 가스크로마토그래피를 이용해 분석한 결과, 11.8%의 산소함량, $-6^{\circ}C$의 유동점, 크로마토그램의 패턴 결과 식물성기름으로 판단하였다. 또한 어떤 식물성 기름인지 알기 위해 HPLC를 이용해 구성성분을 분석한 결과, LLO, OOL, POL이 높은 비율로 분석되어졌으며, 이는 대두유가 주성분이면서 다른 식물성기름의 혼합형태인 폐유로 최종 판단하였다. 본 연구를 통해 향후, 불법 투기되는 유류에 대한 유종 구분 및 오염원인자 판단을 위해 활용 가능할 것으로 판단된다.

• 한국수소및신에너지학회논문집
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• 제22권1호
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• pp.83-91
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• 2011

We investigated the effect of composition of a sulfur electrode with MWNT on the discharge behavior and cycling property of a Li/S cell. The MWNT content of a 60wt.% sulfur electrode varied from 10 wt.% to 30 wt.%. The optimum content of MWNT is 20wt.%, which shows the best cycling property. The first discharge capacity is 1166 mAh/g and decrease to the 542 mAh/g after 30th cycle. The homogeneous distribution of MWNT is an important factor for cycling properties.

• 한국게임학회 논문지
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• 제12권4호
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• pp.65-75
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• 2012

본 연구는 Xbox-360게임 "댄스센트럴"을 통해 댄스게임의 신체적 정서적 무용교육효과를 살펴보았다. 이에 질적연구 방법 중 사례연구방법을 선정하여 댄스게임 무경험자이자 N세대에 속하는 성인 8인을 동일집단대상으로 연구하였다. 연구는 2011년 7월 18일부터 8월 15일까지 5주 동안 총 15회 댄스게임 체험을 통해 관찰자료, 반구조화 면담, 자기보고서, 참여영상을 수집하여 분석하였다. 그 결과 신체적('선행된 리듬의 익숙함', '반복된 동작의 표현성 향상', '신체활동이 활발한 댄스게임'), 정서적('단계변화에 따른 자신감 형성과 몰입 변화', '스트레스 해소', '중독성 없는 댄스게임')으로 도출되었다.

• 한국과학교육학회지
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• 제23권1호
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• pp.57-65
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• 2003

이 연구에서는 계획과 검토 단계를 강조한 4단계 문제 해결 전략을 사용하는 해결자 청취자 활동에서 내 외향성에 따른 소집단 구성의 효과를 조사하였다. 수업 전 고등학교 이과 2학년 3개 학급(N=87)을 대상으로 내 외향성 검사를 실시한 후, 내 외향성 동질 집단과 이질 집단, 그리고 통제 집단으로 무선 배치하였다. 연구 결과, 화학 문제 해결력 검사의 점수는 두 처치 집단이 통제 집단보다 유의미하게 높았으나, 학습 곤란도와 자야효능감에서는 집단 간 유의미한 차이가 없었다. 수업 처치에 대한 의식의 하위 항목들에서는 두 처치 집단 간 유의미한 차이가 없었으나, 청취자의 역할 수행에 대한 인식, 문제 해결 전략과 해결자 청취자 활동에 대한 선호도에서 외향적인 학생의 점수가 내향적인 학생의 점수에 비해 유의미하게 높았다.

• 동아시아식생활학회지
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• 제24권6호
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• pp.915-923
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• 2014

This study investigated organic coffee choice motives from a coffee shop market segmentation perspective in order to understand the potential importance they may have upon attitudes and behavioral intentions to buy organic coffee. A factor-cluster segmentation approach was used for this study. An exploratory factor analysis identified five organic coffee choice motives: 'Sensory', 'Environment', 'Trust', 'Health' and 'Price'. Based upon these five choice motives, cluster analyses classified all respondents into three homogeneous subgroups: 'Highly motivated', 'Moderately motivated' and 'Unmotivated'. Analysis of variance tests indicated that attitudes and intentions to purchase organic coffee were significantly different among the three clusters. In particular, two cluster groups representing 'Highly motivated' and 'Moderately motivated' were found to offer the most utility for further organic coffee market segmentation research. Especially, due to perceptions about high price premium of organic coffee, the 'Moderately motivated' group had higher positive attitudes, although, their intentions to buy organic coffee were not higher than those of the 'Unmotivated' cluster. Findings support previous research propositions that high price could be the strongest barrier for people to purchase organic products including the organic coffee business context. This will assist to market and promote pricing strategies for caf$\acute{e}$s and restaurants to optimize organic coffee sales revenue. Implications for all cluster groups regarding unique socio-demographic characteristics and behavioral intentions are discussed. Organic coffee marketers can apply these findings towards the development of effective target market strategies.

• 청정기술
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• 제23권2호
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• pp.148-157
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• 2017

To prepare UV-curable polyurethane-acrylate oligomer, NCO-terminated urethane prepolymers with trimethylolpropane, [TMP; 0 (0), 0.1 (0.021) and 0.2 (0.043) mole (mole fraction)] as crosslinkable tri-functional chain extender were end-capped with pentaerythritol triacrylate [PETA; 2.0 (0.400), 1.7 (0.354) and 1.4 (0.304) mole (mole fraction)] with one hydroxyl group/three vinyl functionalities. The stable as-formulated UV-curable polyurethane-acrylates [stable mixtures of PETA-capped oligomer/reactive acrylic monomer diluents without/with heptadecafluorodecyl methacrylate (PFA; 0, 6 and 9 wt%)] were formed up to 0.2 (0.043) mole (mole fraction) of TMP content in the prepolymer, while homogeneous-mixing failed at 0.3 (0.068) mole (mole fraction), in which the crosslink density in NCO-terminated urethane prepolymer was too high to enable the formation of stable mixture. This study examined the effect of TMP/PETA molar ratio and heptadecafluorodecyl methacrylate (PFA) content (wt%) on the properties of UV-cured polyurethane-acrylates as marine antifouling coating materials. The properties of UV-cured polyurethane-acrylate were found to be significantly dependent on the crosslinkable TMP/PETA ratio and PFA content. With the increasing of the TMP and PFA contents, the contact angles increased, and consequently the surface tension decreased. The adhesion of algae/barnacles to PFA contained film samples were found to be sufficiently weak to allow their easy removal. These results suggest that the UV-cured samples containing PFA have strong potential as coating materials for antifouling applications.