• Journal of Microbiology
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• 제45권6호
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• pp.485-491
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• 2007

The effects of biological pretreatment on the Japanese red pine Pinus densiflora, was evaluated after exposure to three white rot fungi Ceriporia lacerata, Stereum hirsutum, and Polyporus brumalis. Change in chemical composition, structural modification, and their susceptibility to enzymatic saccharification in the degraded wood were analyzed. Of the three white rot fungi tested, S. hirsutum selectively degraded the lignin of this sortwood rather than the holocellulose component. After eight weeks of pretreatment with S. hirsutum, total weight loss was 10.7%, while lignin loss was the highest at 14.52% among the tested samples. However, holocellulose loss was lower at 7.81 % compared to those of C. lacerata and P. brumalis. Extracelluar enzymes from S. hirsutum showed higher activity of ligninase and lower activity of cellulase than those from other white rot fungi. Thus, total weight loss and changes in chemical composition of the Japanese red pine was well correlated with the enzyme activities related with lignin- and cellulose degradation in these fungi. Based on the data obtained from analysis of physical characterization of degraded wood by X-ray Diffractometry (XRD) and pore size distribution, S. hirsutum was considered as an effective potential fungus for biological pretreatment. In particular, the increase of available pore size of over 120 nm in pretreated wood powder with S. hirsutum made enzymes accessible for further enzymatic saccharification. When Japanese red pine chips treated with S. hirsutum were enzymatically saccharified using commercial enzymes (Cellulclast 1.5 L and Novozyme 188), sugar yield was greatly increased (21.01 %) compared to non-pre treated control samples, indicating that white rot fungus S. hirsutum provides an effective process in increasing sugar yield from woody biomass.

• Advances in environmental research
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• 제9권1호
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• pp.55-64
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• 2020

The problems with unsorted municipal waste are always associated with disposal issues as it requires a large area for landfilling or high energy used for incineration. In recent years, an autoclaving technique has been considered a promising approach which could minimize the volume of organic waste from being directly disposed or incinerated. In this work, an attempt was done to study the saccharification potential of organic residues under elevated temperature Thermal treatment involving hot water bath was applied to treat the organic residue ranging from 60℃ to 100℃ for 30 and 60 minutes. The result obtained showed an increasing trend for the concentration of glucose and carbohydrate. However, the result for lignocellulose content which contains various component includes extractive, holocellulose, hemicellulose, cellulose and lignin show variation. Based on the thermal treatment carried out, the result indicated that the trend of glucose and carbohydrate content. The highest percentage of glucose that can be obtained 978.602 ㎍/ml which could be obtained at 90℃ at 60 minutes. The carbohydrate also shows an increasing trend with 0.234 mg/ml as the highest peak achieved at 80℃ for 30 minutes treatment. However, it was found that the lignocellulose content varies with temperature and time. The statistical analysis was carried out using two-ways ANOVA shows an interaction effect between the independent variables (temperature and contact time) and the saccharification effects on the food wastes. The result shows a variation in the significant effect of independent variables on the changes in the composition of food waste.

• 펄프종이기술
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• 제42권2호
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• pp.53-60
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• 2010

Kudzu vine (Pueraria lobata) is a perennial plant and spreads all around in South Korea. Recently the use of leaves, stems and roots of kudzu vine has been investigated in many fields. However, the research on kudzu fibers has not been performed in korean pulp and paper industry. As the stems and roots of kudzu were estimated to contain cellulosic fibers, the possibility of producing a raw material from kudzu fibers in pulp and paper mills was investigated in this study. The stems and roots of kudzu were collected in woods, and then the chemical composition and ash contents was measured. To acquire kudzu pulp, kraft pulping and bleaching of kudzu roots were carried out sequently. After making kudzu pulp, freeness and fiber length were measured, and handsheets was also prepared with kudzu pulp and the properties of handsheets were determined. Consequently, kudzu fibers have lower holocellulose contents than wood fibers and other non-wood fibers. Average fiber length is shorter than that of Sw-BKP, but shows the same level as that of Hw-BKP. The handsheet made from kudzu pulp shows the bulkier structure than those made from Hw-BKP and Sw-BKP.

• 한국산림과학회지
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• 제99권2호
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• pp.226-234
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• 2010

This study was conducted to examine the effects of pruning season on the compartmentalization of pruning wounds in Acer palmatum and Pinus strobus. A total of eighty five field-grown trees for each species were allocated to five different seasons, early- and late-winter, mid-spring, mid- and late-summer, for pruning treatments. Wound closure rate (WCR) of the two species for one year after treatment, area of discolored stem tissue on the medial longitudinal surface and cambial dieback length under the pruning wound of A. palmatum were measured. Changes of total phenols and variations of extractives, holocellulose and lignin at the treated branch unions were examined. In WCR of A. palmatum, late-winter (March, 39.8%) and mid-spring (May, 39.7%) were higher than any other seasons, while early-winter (November, 28.4%) was significantly lower than late-winter and mid-spring. P. strobus showed similar results with A. palmatum. The WCR of early-winter (57.2%) was the lowest significantly among the five seasons, and mid-spring (73.5%) and late-winter (71.4%) showed higher a WCR than other seasons. In the discolored/wound area ratio of A. palmatum, early-winter (73.2%) was the highest by far, and mid- (July) and latesummer (September, 36.7%, respectively) were the lowest among the five seasons. In the length of cambial dieback, two dormant seasons, early- and late-winter were longer than any other seasons. Phenol contents at the treated branch union were changed in line with the seasonal fluctuation of the tree. Total phenols in the below core of the treated union were higher than those of the branch union with living branch, while little differences were seen in the above core. At the branch core of the treated union, phenols of A. palmatum decreased one month after the treatments, but P. strobus maintained similar to or a little higher than those at the controls. The major changes in chemical composition at pruning wounds were extractives and lignin increased by less than 20% in A. palmatum, while extractives in P. strobus remarkably increased by 70%.

• Journal of Plant Biology
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• 제30권2호
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• pp.135-149
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• 1987

An experiment was made in order to analyze the growth characteristics and productivity of Zoysia japonica under control, mowing and TIBA treatment conditions at the experimental farm of Kyung Hee University. The field was planned by the split plot design method and each treatment was given to two plots (40$\times$40 and 100$\times$100 mm) and was replicated three times. Each plot was 9$m^2$ for Zoysia japonica. The sampling of each plot was taken once a week after sowing. In order to know the dry matter of total standing crops, each organ of plants was kept at 9$0^{\circ}C$ and weighed. Total leaf area of a plant was measured by drawing method. The author adopted the growth analysis of English School. Holocellulose analysis by sodium chlorite method was made. The increasing rate of LAI was high in all plots between 10th and 12th week after sowing and high growth rate of assimilatory organ was observed in low density area of TIBA plot. Between 10th and 12th week after sowing all the experimental plots showed high increasing rate of standing crop and in the process of growth each density area of TIBA plot recorded high increasing rate. In all the plots F/C ratio and RGR were high when the growth rate of assimilatory organ was increasing cosiderably, and the higher the planting density was, the higher F/C ratio was recorded. In all the plots NAR recorded maximum rate before the growth period to show a high increase of RGR. In the process of the growth TIBA plot showed high icnreasing rate of NAR. CGR showed high value in high density areas of all the plots and reached its maximum in the 13th week after sowing. Compared to the other experimental areas the low planting density areas of control plot and TIBA plot showed considerable earlier lignification indicating low Holocellulose content in the growth process.

• 펄프종이기술
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• 제46권5호
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• pp.79-87
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• 2014

The enrichment with nutrients in rivers and lakes becomes serious problems in Korea. As the environmental friendly solution, the phytoremediation are getting more attention and the water hyacinth could be a strong candidate plant for this. Water hyacinth is a free floating freshwater plant proven to be a effective plant used for a purification of a polluted lakes, sewage wastewater, and livestock wastewater. The biggest obstacles for application of water hyacinth in Korea could be the disposal of the water hyacinth before winter, because there is no feasible use for the water hyacinth until now. In this study, the characteristics of water hyacinth were deeply investigated for providing background data to develop appropriate application of the water hyacinth. The water hyacinth was fractioned three parts, leaf, air blader, root of which morphological properties were evaluated with microscope and field emission scanning electron microscope (FE-SEM). The water contents of each parts of the water hyacinth were 87% for leaf, 96.5% for air bladder, 94.6% for root. The chemical compositions of each parts were also measured and showed around 29% of hot water extract in leaf and air bladder, 42% holocellulose for leaf, 47% holocellulose for air bladder. The application of water hyacinth biomass without root parts to papermaking provided bulkier structure, but the tensile strength was decreased.

• Journal of the Korean Wood Science and Technology
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• 제47권2호
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• pp.189-199
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• 2019

This study addresses the biorefinery feedstock from Pinus densiflora. This raw material is a major tree species in the Republic of Korea; it is renewable, has cost-effective, and is readily available. In this study, steam explosion of P. densiflora was performed in a reactor at $225^{\circ}C$ and with 1 to 13 min reaction times with or without previous water impregnation. The combined severity factor (Ro), which is an expression relating the reaction temperature and reaction time used in the steam explosion treatment, ranged from 3.68 to 4.79. The influence of both impregnation and steam explosion conditions were investigated by examining color variations, chemical composition, and mass balance on the pretreated solids. The results showed that steam-exploded P. densiflora that was not impregnated with water exhibited significantly darker color (chroma 28.8-41.4) than water-impregnated and steam-exploded P. densiflora (chroma 18.8-37.3). The increased ${\alpha}$-cellulose and lignin contents were detected as the severity factor increased. Furthermore, the ${\alpha}$-cellulose and lignin contents in the non-impregnated/steam-exploded P. densiflora were higher than those in the water-impregnated/steam-exploded P. densiflora. However, the decreased holocellulose content was detected as the severity factor increased. In mass balance, the holocellulose yield from water-impregnated/steam-exploded P. densiflora was higher than that from the non-impregnated P. densiflora.

• Weed & Turfgrass Science
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• 제3권4호
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• pp.253-261
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• 2014

본 연구는 지칭개, 박주가리, 큰부들, 띠 종자섬유에 대한 간단한 화학적, 물리적 특성을 파악하고 이의 활용가능성 여부를 알아보기 위해 수행되었다. Holocellulose 함량은 건조중의 74-88.5%로서 박주가리 종자섬유가 가장 높았고 전체적으로 큰부들 줄기의 것(59.5%)보다 높은 경향이었다. 그러나 holocellulose에 대한 alpha-cellulose의 비율은 45-48%로서 종자섬유간 서로 비슷하였다. 리그닌 함량은 17.0% (띠)-24.0% (박주가리), 회분은 0.22% (박주가리)-4.2%(띠), 열수추출물은 2.2% (지칭개)-7.8% (띠), 유기용매 추출물은 0.4% (띠)-6.3% (부들) 함량을 나타내었다. Crystallinity index (CI) 분석에 있어서는 무처리 종자섬유의 경우 지칭개와 박주가리가 약 65%로서 높았고, 띠와 큰부들은 약 54%로서 상대적으로 낮았다. 그러나 화학처리후 얻어진 EDA 섬유간의 CI는 박주가리가 71.8%로서 가장 높았고 미소한 차이이지만 큰부들과 지칭개는 보다 낮아 각각 69.3%, 67.2%를 나타내었다. 한편 열분해 특성은 전형적인 lignocellulose계 패턴을 보였는데 무처리 종자섬유의 경우, 가장 높은 분해율을 나타낸 온도는($%/^{\circ}C$) 박주가리, 큰부들, 지칭개, 띠에서 각각, $312^{\circ}C$, $321.8^{\circ}C$, $331.5^{\circ}C$, $341.6^{\circ}C$로서 박주가리가 가장 낮고, 띠에서 가장 높은 편이었다. 그런데 EDA 섬유의 경우는, 지칭개, 큰부들, 박주가리, 띠에서 각각 $327^{\circ}C$, $327^{\circ}C$, $341.1^{\circ}C$, $360.0^{\circ}C$로서 띠가 가장 높은 편이었다. 이상의 결과들은 종합해 볼 때, 본실험의 종자섬유는 그 자체로 직접 이용할 수 있을 정도의 화학적, 물리적 특징을 가졌으나 일련의 화학처리를 하면 보다 우수한 품질의 섬유를 확보할 수 있으며, 이들은 여러 용도의 천연섬유 자원으로 유용하게 활용될 수 있을 것으로 판단되었다.

• Journal of the Korean Wood Science and Technology
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• 제18권2호
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• pp.8-15
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• 1990

This study was carried out to investigate the wood qualities for the end-use development of sycamore (Platanus occidentalis L.) grown in Korea. The results obtained were as follows: 1. The average length of wood fibers was l.56mm and the average width of annual rings was 9.5mm. It had very fast growth rate. 2. The specific gravity in air-dry was 0.66. The shrinkage and water absorption were relatively large. The shear and impact strengthes were very strong, while the compressive, tensile, and bending strengthes were weak in comparison to it's specific gravity. 3. The contents of ash, holocellulose, lignin were relatively high 0.74%, 83.08%, 28.79%, but that of pentosan was low 18.53%. 4. The expected uses of sycamore wood are plywood. fancy veneer, small furniture, musical instrument, door and window frame, tool handels, boxes, etc.

• Journal of the Korean Wood Science and Technology
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• 제18권2호
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• pp.3-7
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• 1990

The chemical Composition of Chinese red pine (Pinus massoniana) submerged in the Yellow Sea for more than 700 years has been examined. When compared to the recent wood, the marked chemical changes in the waterlogged wood is the higher amount of lignin with lesser amount of holocellulose and abnormally high ash content. In the heavily degraded samples, the degradation of cellulose is more severe than that of hemicellulose. However, hemicellulose is much more attacked than the cellulose at the initial stage of deterioration in the sea water. Chemical analysis suggests that the cellulolytic marine microorganisms, whether they are fungi or bacteria. can be regarded as the primary agents for the destruction of the archaeological woods submerged in the sea water.