• Clinical and Experimental Reproductive Medicine
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• 제29권1호
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• pp.45-56
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• 2002

Objectives: Recently, recombinant FSH (rFSH) has been manufactured using a Chinese hamster ovary cell line transfected with the gene encoding human FSH. Both rFSH and urinary gonadotropin (uFSH) could be used for controlled ovarian hyperstimulation (COH). However, uFSH implies a number of disadvantages, such as batch-to-batch inconsistency, no absolute source control, dependence on large amounts of urine, low specific activity, and low purity. The purpose of this study was to evaluate the efficacy of rFSH in human IVF-ET program. Materials and Methods: A total of 508 infertile women was enrolled in this study. They are classified into rFSH group (n=177) or uFSH group (n=331), and all of them were matched by age and cause of infertility in same period. The $Puregon^{(R)}$ (Organon, Holland) was used as rFSH, and the Metrodin-$HP^{(R)}$ (Serono, Switzeland) and $Humegon^{(R)}$ (Organon, Holland) was used as uFSH. We subdivided the patients into three age groups. The outcomes of IVF-ET program were analyzed using the statistical package for social sciences (SPSS). Results: There was no significant differences in the level of estradiol on hCG injection day, the numbers of retrieved oocytes, matured oocytes, fertilized oocytes, transferred embryos, frozen embryos between the two groups. The total dose (IU) of gonadotropin for COH was significantly lower in the rFSH group compared to uFSH group ($1339{\pm}5491.1$ vs $2527.8{\pm}1075.2$ IU, p<0.001). Clinical pregnancy rate per embryo transfer in the rFSH group showed increasing tendency, compared to the uFSH group, but there was no statistical significance (35.2% vs 29.3%). Our results demonstrated that the relative efficiency of rFSH compared with uFSH is higher in older patients. Conclusions: The ovarian stimulatory effect and clinical outcome of recombinant FSH was similar to that of the urinary gonadotropin. The IVF-ET cycles with significantly lower dose of gonadotropin in rFSH group showed comparable results. Therefore, we suggest that recombinant FSH is more potent and effective than urinary gonadotropin.

• Asian-Australasian Journal of Animal Sciences
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• 제13권9호
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• pp.1205-1209
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• 2000

The present study was undertaken to investigate the effect of stimulation of follicular development with eCG on the peripheral levels of inhibin and FSH in Murrah buffaloes. Estrus was synchronized in five normally cycling females by insertion of Crestar (Intervet, Boxmeer, Holland) implants for nine days. Estradiol valerate was administered i.m. on the day of implant insertion. On the 10th day of the induced estrous cycle a single dose of 3000 IU eCG (Folligon, Intervet, Boxmeer, Holland) was given, followed by treatment with 25 mg of $PGF_2$ alpha (Lutalyse, Upjohn, Belgium) 48 h later. Blood samples were obtained during the induced estrus, on cycle day 10 (luteal phase), at the superovulatory estrus (43 h after PGF) and during the periovulatory period (64 h after PGF). Ultrasonography was done daily to monitor follicular development. Plasma concentrations of inhibin and FSH were determined by specific radioimmunoassays. Differences between $mean{\pm}SEM$ values of different phases of the cycle were compared by ANOVA. The mean number of small (2-5 mm), medium (6-9 mm) and large (>10 mm) follicles observed two days after eCG treatment and on the day of superovulatory estrus was $2.8{\pm}0.31$, $5.2{\pm}0.30$ and $1.4{\pm}0.09$ and $1.9{\pm}0.21$, $2.8{\pm}0.40$ and $5.0{\pm}0.83$, respectively. The mean number of ovulations was $3.6{\pm}0.37$ and the mean number of unovulated follicles was $6.1{\pm}0.47$. Most of the follicles >10 mm in diameter had ovulated (72%). The mean ${\pm}SEM $ of plasma inhibin concentration $(2584.15{\pm}17.92pg/ml)$ during the superovulatory estrus was significantly higher $(p{\leq}0.05)$ than during the induced estrus $(749.87{\pm}17.29pg/ml)$, the luteal phase $(1099.54{\pm}24.98pg/ml)$ and periovulatory period $(1682.71{\pm}29.88pg/ml)$, respectively. $Mean{\pm}SEM$ plasma FSH concentration during the induced estrus $(10.35{\pm}0.41ng/ml)$ was not different from that during the superovulatory estrus $(8.52{\pm}0.39ng/ml)$, but was significantly higher $(p{\leq}0.05)$ than during the luteal phase $(2.81{\pm}0.42ng/ml)$ and periovulatory period $(5.7{\pm}0.28ng/ml)$. These data indicate that treatment with eCG in buffaloes for inducing superovulation results in a significant elevation in plasma inhibin levels and a decrease in plasma FSH levels during the superovulatory estrus. Thus, we suggest that the elevated plasma inhibin coming from fully developed follicles continued for a long time which results in inhibition of FSH leading to poor ovulation in the remaining follicles, which may be the cause of suboptimal superovulatory response.

• 한국과학교육학회지
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• 제41권3호
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• pp.267-281
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• 2021

참여로서의 학습에 대한 관점의 전환은 실행 과정에서의 학습자의 정체성 변화 및 발달(identity world, 정체성 작업)에 관심을 두게 한다. 본 연구는 정체성의 지속적이고 유동적인 과정에 관심을 두며, 그에 따라 정체성 작업이 일어나는 학습자를 둘러싼 구조와 그 구조 속에서 개인의 행위 및 행위력의 발현에 초점을 둔다. 이러한 관점에서 구조, 행위력, 정체성을 변증법적으로 이해하기 위한 이론적 도구로써 Holland et al.(1998)의 실행 과정에서의 정체성(identity-in-practice) 이론을 소개한다. 이 이론을 대표하는 개념은 '인식된 세계', '위치성', '자아 저술'이다. '인식된 세계'는 인간의 행위에 의해 지속적으로 재구성되는 의미의 그물망이자 동시에 인간의 행위를 조각하는 사회적 힘으로 이해되며, '위치성'은 이 세계 속에서 자신의 사회적 위치에 대한 이해를 의미한다. '자아 저술'은 위 두 개념을 종합하는 차원으로서 개인은 인식된 세계 속에서 실행을 통해 다양한 사회적 목소리를 협연하여 자아를 스스로 형성해간다는 의미를 담고 있다. 본 연구는 이 개념을 바탕으로 과학 교육의 선행 연구 분석을 통해 이 이론의 다양한 활용 사례를 기술했다. 그러나 사회문화적 접근을 취하는 이 이론의 특성상 해외 연구 사례가 단순히 우리에게도 적용될 수 없기에, 국내 상황에서 본 이론이 활용될 수 있는 방향을 논의하여 방법론적 도구로서 이 이론의 적용가능성을 확장하고자 하였다. 다층적이고 지속적이며 누적적인 정체성 작업을 강조하는 본 이론의 도입이 시대적으로 요구되는 과학 교육 개혁의 지평을 넓히는 자원으로 활용되기를 기대한다.

• 한국심리학회지:학교
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• 제18권3호
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• pp.371-398
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• 2021

본 연구는 A 대학 4학년 학생선수들을 대상으로 3회기에 걸친 진로프로그램을 시행하고 그 효과성을 검증하였다. Super와 Ginsberg의 진로발달이론과 Holland의 직업선택이론을 기반으로 하여 진로프로그램을 구성하였으며 90분씩 3주에 걸쳐 진행됐다. 연구에 참여한 학생선수는 총 13명이며 운동 종목은 야구, 럭비, 하키, 피겨이다. 학생선수는 운동을 그만두었을 때 운동선수 이외의 다른 직업에 대한 준비가 쉽지 않다는 문제점에 기반하여 본 프로그램은 학생선수들이 졸업 후 가질 수 있는 직업들은 무엇인지 탐색할 수 있게 하고 그를 통해 진로 준비의 효능감을 높이는 것을 주목적으로 하였다. 프로그램을 통해 학생선수들의 자기 이해가 향상되었으며 진로 태도 성숙의 준비성이 향상되었다. 또한, 학생선수들의 진로결정 자기효능감이 프로그램 시행 전과 비교하여 유의미하게 향상되었다. 그러나 학생들의 진로 스트레스는 프로그램 전과 비교하여 유의미하게 줄어들지 않았다. 비교적 단기간의 진로프로그램이지만 학생선수들이 학령기 동안 경험하기 힘들었던 자신에 대한 이해와 직업정보에 대한 탐색은 그들의 진로준비에 효과적으로 도움이 된다는 것이 발견되었다. 마지막으로 학생선수들을 위한 진로프로그램의 개발과 시행의 필요성과 본 연구의 의의 및 한계점 등이 논의되었다.

• 한국체육학회지인문사회과학편
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• 제55권6호
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• pp.661-676
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• 2016

이 연구의 목적은 무용전공자들을 위한 진로개입의 체계적인 접근 방안을 제시하는 것이다. 의사결정의 종합적인 평가와 합의를 도출하기 위하여 델파이와 계층구조분석(AHP)을 실시하였다. 연구결과, 총 4개 영역의 16개 요소가 도출되었다. 직업지도에서는 '다양한 무용직업 세계의 이해', 진로교육에서는 '구체적인 진로설계와 목표설정', 진로상담에서는 '삶과 연계한 무용진로의 내러티브 지도', 진로개입 네트워크에서는 '무용일자리 박람회 및 워크숍 정례화'를 각각 가장 중요시하는 것으로 나타났다. 모든 요인의 복합가중치에서는 진로설계와 목표설정, 다양한 무용직업 세계의 이해 등 전반적으로 진로교육과 직업지도가 중요하게 요구되고 있는 것으로 확인되었다. 이를 토대로 무용진로개입의 방안은 다양한 무용직업과 이중경력을 통한 진로대안의 확장, 다각화된 무용진로개발로드맵의 교육과정 적용, 무용진로개입 진단도구 및 성과준거의 개발, 체계적인 진로개입을 위한 무용교육자들의 전문교육과 인식제고, 민·관·학의 네트워크 조직화 등이 제시되었다. 이 연구는 Holland(1997)의 진로탐색이론, Super(1990)의 진로발달이론, 그리고 Savickas(2005)의 진로구성이론 등 진로개입의 다양한 관점에 기초하여 무용전공자를 위한 진로개입의 역동적인 실제와 체계적인 접근 방안에 대하여 논의하였다.

• 대한물리치료과학회지
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• 제15권1호
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• pp.57-66
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• 2008

This study was conducted to investigate the effects of kinesio taping in patients with degenerative arthritis. 40 female patients with degenerative arthritis were voluntarily recruited and randomly divided into experimental and control group. Experimental group(mean age was 73.4 years, height was 153.5cm, and weight was 59.9kg) was applied patellar inferior and medial gliding, quadriceps, tibial external rotation, gastrocnemius, and soleus using a kinesio tape. And control group(mean age was 72.7 years, height was 154cm, and weight was 58.6kg) was received a interferential current therapy(ENDOMED 582, Enraf－Nonius, Holland). And we received a consent form from all subjects. Measuring was conducted by using a 3－dimensional gait analysis system for measuring of stride length, cadence, and velocity. The results were as follow: Stride length was significantly increased after than before treatment(p<.05). Cadence was significantly increased after than before treatment(p<.05). And Velocity was significantly increased after than before treatment(p<.05). Cadence was a significant difference between experimental and control group(p<.05), but there was no significant difference stride length and velocity between two groups. So we suggest that kinesio taping has a effectiveness in patients with degenerative arthritis.

• KIEAE Journal
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• 제11권5호
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• pp.3-12
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• 2011

Industrialization and development of technology satisfied various humanly needs and lay the basis of numerous benefit and profit. New technologies like these mostly required large amounts of fossil Fuel, Fossil energy depletion rate was increasing rapidly. However, technical development for Human race required absolute sacrifice of the environment. Especially, 'City' which had been focused as stage of human activities, allowed to continue to have fossil energy dependent activities, and it shows in many data that the city is responsible for the 75-80% of the green house gas by human. In order to solve the problem relating climate changes and energy, European countries already made progress studies on many of the low carbon green city and pilot case construction. Especially, Germany, Austria, Holland, Sweden who had been playing leader role on environmental awareness and ecological concept, are actively constructing low carbon green city project based on Ecological city planning scheme. These projects positively utilize planning scheme that limits carbon emission using Urban Metabolism concept. Therefore, throughout this study, I would like to present planning and direction of future domestic low carbon green city by analyzing theories relative theories and best practices in Europe.

• 대한치과보철학회지
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• 제42권2호
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• pp.167-174
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• 2004

Statement of problem: Removable partial denture and complete denture often require denture base relines to improve the fit against the tissue-bearing mucosa because of gradual changes in edentulous ridge contours and resorption of underlying bone structure. Purpose: This study was performed to investigate the effect of surface design on bond strength of relining denture base resins to denture base acrylic resin. Materials and method: Heat curing resin(Lucitone 199, Dentsply U.S.A. and Vertex, Dentimex, Holland), self curing resin(Tokuso rebase, Tokuyama, Japan), and visible light curing resin(Triad, Dentsply, U.S.A.) were used in this study. The surface designs were classified as butt, bevel and rabbet joint and the bond strengths were measured by Universial Testing Machine (Zwick 2020, Zwick Co., Germany). Results and Conclusion: The obtained results from this study were as follows ; 1. The bond strength of Vertex resin was higher than those of Tokuso rebase and Triad. 2. The bond strength of rabbet and bevel joint was higher than that of butt joint. 3. The failure mode of Triad and Tokuso rebase was mainly adhesive, but cohesive failure was shown mainly in vertex.

• 한국사회복지학
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• 제41권
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• pp.7-42
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• 2000

Little empirical study has been conducted concerning social worker' empowerment that has very important function on clients' empowerment. Using the sample of 222 social workers selected from 92 community welfare centers, this study attempted to identify social workers' empowerment level and its predictors. This study used the empowerment scale developed by Leslie, Holzhalb, Holland (1998) that focuses on measurement of social workers's empowerment in social work agencies. Using confirmatory factor analysis, it was confirmed that the scale has three factors such as control of work environment, work relationships, and personal work orientation. This study showed that using 5 points scale, mean of social workers' empowerment is 3.67. In identifying predictors of the empowerment, this study used variables from the following aspects: organizational characteristics, job characteristics, and individual characteristics. Using regression analysis, it was found that variables related to job characteristics such as role ambiguities, role conflicts, and skill varieties are statistically very significant predictors of social workers' empowerment. Self-esteem in personal characteristics and participation in decision making and transformational leadership in organizational characteristics are also statistically significant predictors of social workers' empowerment. This study also revealed that self-control and self-esteem variables function as moderator in the relation with variables such as role ambiguities and role conflicts. Finally, this study discussed the direction of future research in social workers' empowerment and the areas that management efforts should be focused on for promoting social workers' empowerment.

• 한국미생물·생명공학회지
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• 제19권6호
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• pp.568-574
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• 1991

효모에서 대랑의 물질생산계를 구축하기 위하여 먼저 여러 종류의 베터의 이용이 가능한 다양한 영양요구성 marker를 지니며 형질전환율이 향상된 효모숙주를 선별 개량하였다. 벡터의 제작에 사용되는 프로모터로는, 효모의 여러 유전자 중에서 그 활성이 매우 높은 해당계의 효소 GAP-DH의 구조 유전자 GAP를 이용하기로 하여, 효모염색체 DNA중에서 GAP 프로모터를 분리하여 이용하기 쉽게 변형하였다. 분리된 GAT promoter의 기능을 검토하기 위하여, reporter로 APase의 구조유전자 PHO5'를 이용하여 세포내의 copy수가 상이한 발현 벡터를 제작하여 GAP 프로모터에 의한 APase의 활성 및 전사산물을 측정한 결과, 정상적인 전사가 이루어 졌으며, 효소활성도 높게 나타났으며, 벡터의 copy수에 의한 효소활성의 차이도 감지되었다.