• Title/Summary/Keyword: Hokkaido University

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Leek Yellow Stripe Virus Can Adjust for Host Adaptation by Trimming the N-Terminal Domain to Allow the P1 Protein to Function as an RNA Silencing Suppressor

  • Sasaki, Jun;Kawakubo, Shusuke;Kim, Hangil;Kim, Ok-Kyung;Yamashita, Kazuo;Shimura, Hanako;Masuta, Chikara
    • The Plant Pathology Journal
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    • v.38 no.4
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    • pp.383-394
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    • 2022

  • In Japan, the P1 protein (S-type) encoded by leek yellow stripe virus (LYSV) isolates detected in Honshu and southward is shorter than the P1 (N-type) of LYSV isolates from garlic grown in Hokkaido due to a large deletion in the N-terminal half. In garlic fields in Hokkaido, two types of LYSV isolate with N- and S-type P1s are sometimes found in mixed infections. In this study, we confirmed that N- and S-type P1 sequences were present in the same plant and that they belong to different evolutionary phylogenetic groups. To investigate how LYSV with S-type P1 (LYSV-S) could have invaded LYSV with N-type P1 (LYSV-N)-infected garlic, we examined wild Allium spp. plants in Hokkaido and found that LYSV was almost undetectable. On the other hand, in Honshu, LYSV-S was detected at a high frequency in Allium spp. other than garlic, suggesting that the LYSV-S can infect a wider host range of Allium spp. compared to LYSV-N. Because P1 proteins of potyviruses have been reported to promote RNA silencing suppressor (RSS) activity of HC-Pro proteins, we analyzed whether the same was true for P1 of LYSV. In onion, contrary to expectation, the P1 protein itself had RSS activity. Moreover, the RSS activity of S-type P1 was considerably stronger than that of N-type P1, suggesting that LYSV P1 may be able to enhance its RSS activity when the deletion is in the N-terminal half and that acquiring S-type P1 may have enabled LYSV to expand its host range.

  • https://doi.org/10.5423/PPJ.FT.06.2022.0077 인용 PDF KSCI

SATELLITE-MEASURED TEMPORAL AND SPATIAL VARIABILITY OF TOKACHI RIVER PLUME

  • Lihan, Tukimat;Saitoh, Sei-Ichi;Iida, Takahiro;Matsuoka, Atsushi;Hirawake, Toru;Iida, Kohji
    • Proceedings of the KSRS Conference
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    • v.1
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    • pp.118-121
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    • 2006

  • Variations in the extent and dispersal of river plume are important in the study of coastal environment. The objectives of this study are to examine relationship between satellite detected plume area and river discharge and to clarify the temporal and spatial dynamic of plume from Tokachi River, Hokaido, Japan. We used 1.1 km spatial resolution of SeaWiFS normalized water-leaving radiance (nLw) images from 1998 to 2002. Supervised maximum likelihood classification was implemented to define classes of surface water optical properties. Satellite observed plume area was correlated to the amount of river discharge from April to October. First mode (44% of variance) of EOF analysis shows the turbid plume distribution resulting from re-suspension by strong wind mixing along the coast during winter. This mode also shows plume distribution along-shelf direction in spring and late summer. Second mode (17% of variance) shows spring pattern across-shelf direction due to strong discharge of snow melting water.

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Separation Performance of Zigzag Air Classifier

  • Hirajima, Tsuyoshi;Nishida, Takuji;Toshima, Ryutaro;Kataoka, Kenji;Tsunekawa, Masami;Asakura, Kuniomi
    • Proceedings of the IEEK Conference
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    • 2001.10a
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    • pp.759-764
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    • 2001

  • The separation performance of zigzag air classifier with angle of 90 degrees was studied using narrow size fractions of thin square samples and granular samples. The simulation results of air velocity inside the classifier indicated that the zigzag geometry induces a new pattern consisting of an upward flow and a circulation flow, Experimental results showed that overflow product recovery was described as an integral calculus of normal distribution as a function of dimensionless air velocity ( $V_{A}$ $V_{A50}$), where $V_{A}$ is superficial air velocity and $V_{A50}$ is the $V_{A}$ at the fifty percent recovery. The $V_{A}$ values were predicted using the equations derived from dynamics for a particle dropping in air. A monitoring system that utilizes changes in acoustic signals emitted during the process of air classification was developed to separate PET with desired recovery or grade. The technical feasibility of the on-line monitoring of the PET recovery and grade was demonstrated by measuring relative energy of the signals.signals.als.

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