• BMB Reports
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• 제29권4호
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• pp.386-392
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• 1996

A Saccharomyces cerevisiae Protein Tyrosine Phosphatase, PTP1, was expressed from an Escherichia coli expression system and milligram quantities of active PTP1 were purified chromatographically. The substrate specificity of the recombinant PTP1 was probed using synthetic phosphotyrosine-containing peptides corresponding to the regulatory phosphorylation sites of the yeast MAP kinase homologues $Fus3_{176-186}$, $Kss1_{179-189}$, and $Hog1_{170-180}$. Peptide sequences derived from the MAP kinase homologues were chosen arbitrarily as starting points for sequence variation studies even though they are not likely to be candidates for physiological substrates of PTP1. Phosphotyrosyl-$Hog1_{170-180}$ peptide showed a $K_M$ value of 877 ${\mu}M$ and phosphorylated $Kss1_{179-189}$ and $Fus3_{176-186}$ peptides showed lower $K_M$ values of 74 ${\mu}M$ and 51 ${\mu}M$ each. To study the effect of sequence variations of the peptide, amino acids of the undecapeptide $Hog1_{170-180}$ (DPQMTGpYVSTR) were sequentially substituted by an alanine residue. More extensive variations of each amino acid revealed positional importance of each amino acid residue. Based on these results, we derived a peptide sequence (DADEpYDA) that is recognized by PTP1 with an affinity ($K_M$ is 4 ${\mu}M$) significantly higher than that of the peptides derived from the phosphorylation sites of Fus3, Kss1, and Hog1.

• 한국축산시설환경학회지
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• 제12권1호
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• pp.29-34
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• 2006

The effects of turning frequency were examined on the efficiency of composting lime treated excess sludge amended with sawdust from the activated sludge process after a liquid/solids separation process. The raw and excess sludge from the activated sludge process associated with the hog wastewater treatment system is a significant problem and composting is an effective method far reducing the pollution potential of hog wastewater sludge. The coagulant used sludge composting and ammonia emissions from composting are not well established. The effect of compost properties such as high total carbon, C/N ratio and pH value on performance of composting sludge and biofiltration of ammonia from composting process were investigated. The ammonia emission was not significantly increased during composting. The ammonia concentrations of the exhaust air of composter were ranged from 0.5 and 7 ppm about 12 days after composting. The performance of the hog wastewater sludge composting was the most sensitive to chemical treated sludge properties such as high total carbon and high C/N ratio of the initial compost mixes. Temperature in compost and ammonia emission were not greatly affected by the turning frequency.

• 대한수의학회지
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• 제22권1호
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• pp.37-52
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• 1982

This study was taken to clarify the histopathological changes of pigs naturally infected with hog cholera. Microscopic observations of the necrotic lesion and inclusion body in the lymphoid organs were carried out in the natural cases of hog cholera and experimental cases inoculated with ALD virus and isolated virus strains. Electron microscopic findings of the intranuclear inclusion bodies in the reticular cell of spleen and lymph node were also observed in the experimental cases. The results obtained are as follow, As the histological findings necrosis of lymphoid organs was observed mainly in the lymph follicle. The necrotic lymphoid organs were found to contain 35.0% in the natural and 37.5% in the experimental cases. Intranuclear inclusion bodies were found mainly in the reticular cells of lymphoid organ, the epithelium of bronchiole and alevolus, and the vascular endothelium of brain. These inclusion bodies were seen in 40.0% of the natural cases and all of the experiment. The inclusion body was appeared to compose of activated nucleoli and chromatin granules (interchromatin and perichromatin) by electron microscopy.

• 대한수의학회지
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• 제6권1호
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• pp.1-9
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• 1966

돈(豚)콜레라는 세계각처(世界各處)에서 널리 유행(流行)되는 돼지의 중요(重要)한 질병(疾病)이다. 그러나 이 질병(疾病)을 혈청학적(血淸學的)으로 진단(診斷)할 수 있는 간단(簡單)하고 고도(高度)로 특이(特異)한 방법(方法)은 아직 없다. 이 연구(硏究)에서는 보체결합반응(補體結合反應)을 이용(利用)한 보다 우수한 혈청적진단법(血淸的診斷法)을 실험(實驗)하여 좋은 성적(成績)을 얻었다. 검출항원(檢出抗原)은 감염돈(感染豚)의 췌장유제(膵臟乳劑)이 양성예(陽性例)에서의 역가(力價)는 1/16 이상이였다. 항혈청(抗血淸)은 조직배양원(組織培養源) 바이러스 재료(材料)를 가토(家兎)에 접종(接種)하여 만들었고 이의 역가(力價)는 1/32 이상이었다. 보체(補體)는 1.1~1.2 충분단위(充分單位)를 사용하였고 감작적혈구(感作赤血球)는 2 단위(單位) 2%로 하였다. 이 방법(方法)으로써 감염돈(感染豚)의 95% 이상이 특이적(特異的)으로 검출(檢出)될 수 있었고, 비감염돈(非感染豚)은 97% 이상이 음성(陰性)으로 검출(檢出)될 수 있었다. 따라서 이 방법(方法)은 돈(豚)콜레라를 진단(診斷)하는데 이용(利用)될 수 있는 보다 낳은 혈청학적(血淸學的) 방법(方法)으로 믿어진다.

• 한국환경보건학회지
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• 제39권6호
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• pp.522-531
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• 2013

Objectives: A field survey was conducted in a hog-raising industry in order to help develop personal protective equipment for workers which would secure the safety and the health of these workers. The attempt by this study will help enhance safety in the livestock industry and contribute to the advancement of the industry. Method: The study first selected a total of 111 workers from the hog-raising industry as research participants and designed a survey with questions on general characteristics, indoor and outdoor working environments, how the workers would in practice wear or purchase the working clothes, what needs to be improved in these new working clothes, how much the workers would be likely to accept the working clothes and protective equipment, and lastly, conditions of the communicable disease control overgarment. The collected data underwent frequency analysis and cross analysis with SPSS 21.0. Result: The research targets' average age was 50 years. Work efficiency by environmental factor was normal, but all age groups had experience of accidents (79.3%). Major wounded parts were under elbow and under knee. Protective equipment most commonly worn was helmet (83.4%), gloves (98.2%) and boots (99.1%), and satisfaction with them was normal at 3.41. Working clothing most commonly worn was old clothing (31.8%) and everyday wear (17.6%) and satisfaction with it was low. Considering the improvement of working clothing, they required attached pouches, elasticity and deodorization. The acceptability of improved working clothing was high at 69.2%. Conclusion: After problems have been addressed in relevant future research, what has been learned from the concerned study will be referred to as a useful basic reference when the relevant field works to develop high-quality working clothing and protective equipment for workers in the hog-raising industry.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 춘계학술대회
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• pp.107-107
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• 2019

Millets are provided considerable amounts of nutrients and gluten-free cereal products and their rich non-nutritional compounds having proven health benefits, especially phenolic compounds. The aim of present investigation was to determine phenolic composition and antioxidant and SOD activity of three different millet of genetic resources namely, foxtail, proso and finger millet. Phenolic compounds were extracted from dehulled grain of genetic resources using methanol and examined for their total phenolic content (TPC), antioxidant activities and superoxide dismutase (SOD)-like activity. The TPC range of hog millet, finger millet and finger millet range from 3.3 to 25.1, 11.1 to 29.0 and 3.8 to 94.3 gallic acid equivalent (GAE)mg/g, respectively. Most of TCP content in dehulled millet grains was distributed from 10 to 20 gallic acid equivalent (GAE)/g, but two accessions of finger millet (IT235690 and 235689) were showed over than 90. The antioxidant activities were measured by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity. Finger millet and hog millet showed 26.4% and 26.7% in the mean of DPPH scavenging activity percentage, but foxtail millet was 13%. The finger millet showed the higher value than hog and foxtail millet in superoxide dismutase (SOD)-like activity. Particularly, two accessions of finger millet (IT235690 and 235689) showed the highest phenolic content and antioxidant activities among the used millet genetic resources and will be primary resources for finger millet breeding to develop the appropriate breeding strategies.

• Asian-Australasian Journal of Animal Sciences
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• 제7권4호
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• pp.523-526
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• 1994

This study was carried out to examine the salting and desalting of small casings from hog and to determine the shelf-life during cold storage. The concentration of salt in the casings equilibrated with that of the added salt after 1 day for 10%, after 2 days for 20% after 7 days for 40% salting level. During desalting at 15 and $30^{\circ}C$, residual salt concentrations in the casings decreased to less than 1% after 1 hour for 10% salt, after 12 hours for 20% salt and after 24 hours for 40% salt. The total colony count of the freshly prepared casing was about log10 4.2. The initial microflora of the prepared casings was dominated by lactic acid bacteria. The higher the salting level, the greater the microbial growth was suppressed during 6 months of storage at refrigerator temperature ($4^{\circ}C$). A salt content of 20% is satisfactory if the casings are being stored for less than 1 month before being used.

• 한국동물위생학회지
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• 제23권2호
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• pp.105-112
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• 2000

This survey was conducted to evaluate the microbiological quality of raw beef and pork products from January to December in 1999. A total of 107 beef and 157 hog carcasses were collected from two abattoirs located in Seoul. The result showed that beef carcasses had an average bacterial loading around 139,000 bacteria/$\textrm{cm}^2$ of carcass surface, indicating a little bit higher count than the results reported in USA and Australian meat. However, overall hygienic status was found to be acceptable for all examined carcasses because 84.4% of product rated excellent, good or acceptable comparable to USA of 91.6% and Australia of 88%. The analysis of data on overnight-chilled to weekend-chilled carcasses indicated that the microbiological growth occurred in the chiller during the weekend chill with increases in total viable count from 130,000cfu/$\textrm{cm}^2$ to 400,000cfu/$\textrm{cm}^2$. Qualitative testing for escherichia coli, EC + MUG was used as a most probable number (MPN) method along with the petrifilm method. The average of MPN/$\textrm{cm}^2$ of E coli biotype 1 was 29MPN/$\textrm{cm}^2$ for beef carcasses and 1,100 MPN/$\textrm{cm}^2$ for hog carcasses, respectively. However, 41% of beef and 16.3% of hog carcasses were shown to be less than < 3 MPN/$\textrm{cm}^2$ in E coli biotype 1 examination. Although salmonella enteritis, S typhimurium and E coli O157:H7 were all negative, listeria monocytogenes was recovered from only one hog surface samples of the 89 carcasses tested.

• 대한수의학회지
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• 제25권1호
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• pp.69-75
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• 1985

Hog cholera serum neutralizing antibody of piglets and sows were titrated by means of END SN method. The piglets of a variety of ages, precolostrally immunized with LOM living HC vaccine were subjected to the test. The sows were vaccinated with lapinized living HC vaccine after 25 days from the parturition. Throughout the studies the following results were obtained and summarised. 1. Hog cholera antibody titers of inbred sows immunized with lapinized living HC vaccine after 25 days from parturition were high except Hampshire group(Table 2). 2. Sows, different stage of the pregnancy or the day of parturition, and of 3 way crossed, that were immunized with lapinized living HC vaccine have shown no significant difference on HC antibody titer(Table 2, 3). 3. HC antibody titers of piglets, immunized with a single dose of LOM HC vaccine before feeding colostrum, were high in case of the younger group(1 week) compare to the older(7 week) (Table 4). 4. The piglets that were booster immunized with LOM HC vaccine at the age of 7 weeks have shown an inconsistent antibody titers(Table 5).

• 대한수의학회지
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• 제25권2호
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• pp.103-112
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• 1985

국내에서 분리된 enterovirus의 병원성을 관찰하기 위하여 1~2일령의 자돈 10두와 35일 령의 유돈 6두에 enterovirus 조직배양부유액을 뇌내 또는 근육내 주사한 후 임상 및 병리학적으로 관찰하였던 바 다음과 같은 결과를 얻었다. 임상적으로는 체온의 상승, 보양창랑, 보행실조, 유약성 마비 및 고도의 삭수가 관찰되었다. 병리조직학적으로는 중추신경계 전반에 걸쳐 수막하 세포침윤, 혈관주위 원형세포침윤, 신경세포의 변성, 미만성과 한국성 gliosis, glial nodule의 형성 등이 관찰되었으며 백질부보다 회백질부에서 다소 심한 경향이었고 배측 신경절염이 전 실험예에서 인정되었다. 한편 hog cholera백신과 enterovirus의 공동주사예에서는 중추신경계의 병변이 enterovirus 단독주사예에 비해 급격히 진행되는 경향이었다. 이상의 소견들을 종합해 볼때 국내에서 분리된 enterovirus는 뇌내 또는 근육내에 접종하였을 때 자돈에서 polioencephalomyelitis를 일으킬 수 있는 병원성이 강한 형으로 추측되며 hog cholera백신과 enterovirus를 공동주사한 예에서 병변의 진행이 급격한 점으로 보아 enterovirus의 자연감염에 hog cholera 백신 등의 stress요인이 작용될 것으로 추측된다.