• 한국동물위생학회지
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• 제41권1호
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• pp.57-61
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• 2018

Canine peripheral nerve sheath tumors (PNSTs) are spindle cell tumors that arise from Schwann cells, perineural cells, fibroblasts or all of them. Based on the morphology and biologic behavior, PNSTs are divided into benign PNST (BPNST) and malignant PNST (MPNST) forms. The aim of this study is to diagnose the two cases of neoplastic tissue samples with features of PNSTs by the histopathology and immunohistochemistry. The study was performed using two specimens from small animal clinic. The first case, A was a mass, 3~4 cm in diameter, extruded from vaginal mucosa of 10-year-old spayed female mixed-breed dog. And the second case, B was a subcutaneous mass, 1.5 cm in diameter, which is originated from right hind leg of 9-year-old castrated male mixed-breed dog. Two cases were stained with hematoxylin and eosin (H&E) for histopathological examination. And also immunohistochemistry (IHC) was performed by the avidin-biotin peroxidase complex (ABC) method with antibodies specific for the following proteins: S-100 protein, smooth muscle actin (SMA) and epidermal growth factor receptor (EGFR). In results, Antoni B schwannoma pattern characterized by pleomorphic, round and fusiform polygonal cells was seen in A. In B, Antoni A pattern, densely packed spindle cells arranged in interlacing bundles was seen in addition to Antoni B pattern. In IHC, cytoplasms of neoplastic cells were diffusely labeled for S-100 expression in A and B. For SMA, both A and B show negative expression. And for EGFR, A shows negative expression but B shows partially positive expression in areas of Antoni B schwannoma pattern. The histopathologic features of two cases coupled with the S-100 immunoreactivity led to a diagnosis of PNST. For SMA, both A and B show negative expression. The diagnosis of A will be a BPNST with the negative result and B will be a MPNST with the positive result for EGFR.

• 대한수의학회지
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• 제39권3호
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• pp.531-537
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• 1999

The present study was carried out to investigate the pathogenicity and pathogenesis of wild rats(Rattus norvegicus), trapped in nature, intranasally infected with Aujeszky's disease virus(ADV/NYJ-1-87) by histopathology, immunohistochemistry and in situ hybridization(ISH). Fifteen rats inoculated intranasally were roughened haircoat, anorexia, listlessness, and depression second day after inoculation, and three rats died in 66-72 hours. Eight rats showed severe pruritus at the face that was accompanied by frequent face-washing movements of the forelegs, and then became violent and spasmodic for an hour or until they died. Four rats slowly recovered after showing mild clinical signs of the disease. Microscopic lesions in infected rats were characterized by meningitis, perivascular round cell infiltration, focal gliosis, and neuronal degeneration and necrosis. And intranuclear inclusion bodies were frequently detected in the cerebral cortex and medulla. Positive reaction to ADV by immunohistochemistry and ISH were detected in the following areas : trigermimal ganglion, brain, tonsil, nasal mucosa, spleen, lung and liver. The result has suggested that ADV intranasally infected in wild rats is followed by replication in epithelial calls of nasal mucosa and tonsil, then invade local lymph nodes by way of the lymphatics. It is also believed that the virus invades bipolar olfactory cells and trigerminal ganglion; and then spread into central nervous system.

• 한국임상수의학회지
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• 제30권4호
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• pp.296-300
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• 2013

7년령의 중성화된 수컷 Yorkshire Terrier가 복강에 종괴가 촉진되어 이를 주증으로 내원하였다. 방사선 검사 상 복강의 등쪽면에 신장 종괴와 작은 복강 종괴가 관찰되었다. 수술적으로 적출된 우측 신장에서는, 육안적으로 붉은 갈색조를 띄는 종괴와 낭상 구조가 함께 관찰되었다. 병리조직검사 상에서 다각형 또는 타원형의 내피세포를 가진 다수의 불규칙한 모양의 신생혈관이 관찰되었으며, 이 혈관들과 종양세포들은 주변조직으로의 강한 침습성을 보였다. 복강의 또 다른 작은 종괴에서는 신장 종괴와 동일한 병리조직학적 소견을 관찰할 수 있었다. 면역조직화학염색을 실시한 결과 종양세포들은 vimentin, CD31 및 von Willbrand factor에 대해 양성 반응을 보였으나, cytokeratin에 대해서는 음성 반응을 보였다. 육안적인 특징, 병리조직학적 소견 및 면역조직화학적 검사를 바탕으로 본 증례는 신장원성 혈관육종으로 진단하였다.

• 한국임상수의학회지
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• 제32권6호
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• pp.548-550
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• 2015

1세령 암컷 고슴도치의 앞다리 피하에서 종괴가 발견되어 내원하였다. 육안적으로 앞다리 피하종괴는 직경 약 4 cm 크기로 융기되어 있었다. 종괴의 단면상에서 중심 부위는 암적색이었으며, 그 주위에 유백색 결절이 무수히 매몰되어 있었다. 병리조직학적으로 진피층에는 다량의 종양세포들이 불규칙한 줄 또는 시트형태를 이루고 있었다. 대부분의 종양세포들은 기저막에 울타리 모양으로 배열되어 있었으며, 종양세포무리의 중심부에서는 건락괴사를 관찰할 수 있었다. 또한 종양세포들은 주변조직으로의 침습성을 보이고 있었다. 면역조직화학염색을 실시한 결과, 종양세포들은 vimentin에는 음성반응을 보인 반면, pancytokeratin에는 강한 양성반응을 보였다. 이상의 육안적인 특징, 병리조직학적 소견 및 면역조직화학적 검사를 바탕으로 본 증례는 기저세포암종으로 진단하였다.

• 한국동물위생학회지
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• 제33권2호
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• pp.199-206
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• 2010

To evaluate the hypoglycemic effect of nano powder propolis, streptozotocin (STZ) induced diabetic rats were divided into 2 groups : diabetic control group and nano powder propolis (0.9ml) group. Then the rats were fed with nano-powder-propolis for 4 weeks. After 4 weeks, oral glucose tolerance test (oral GTT) was performed and blood sugar levels after 16 hours fasting, body weights, and blood lipid levels were measured. Finally, pancreas were collected and examined by histopathology and immunohistochemistry. In conclusion, the nano-powder-propolis was effective in the treatment of diabetes due to the reduction of blood sugar level and the regeneration of damaged ${\beta}$-cells observed in streptozotocin-induced diabetic rats.

• 한국동물위생학회지
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• 제44권4호
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• pp.309-313
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• 2021

A 12-year-old, spayed female, Toy-poodle dog presented in progressive, pruritic carpal mass and mandibular papule. The skin lesions and enlarged superficial lymph nodes were surgically removed. According to histopathology and immunohistochemistry, the patient diagnosed as cutaneous epitheliotropic T-cell lymphoma with lymph node metastasis. Chemotherapy with L-asparaginase, lomustine and prednisolone was initiated. The patient deteriorated despite treatment and was admitted to the hospital. Blood tests revealed significant leukocytosis. Circulating large lymphocytes with convoluted and cerebriform nuclei seen in peripheral blood led to presumption of Sézary cells. Thus, considering skin, lymph nodes and blood involvement, Sézary syndrome was diagnosed.

• 대한족부족관절학회지
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• 제24권4호
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• pp.156-160
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• 2020

Malignant peripheral nerve sheath tumors (MPNSTs) usually arise in soft tissues; they are rarely found in the bone. This paper reports a case of MPNST in the foot and ankle joint involving the distal tibia, talus, calcaneus, navicular, medial intermediate, and lateral cuneiform, cuboid, and 2nd to 4th metatarsal bone. Palliative treatment was performed. The authors encountered a patient with intraosseous MPNST of the midfoot who presented with nonspecific clinical and radiologic findings. This case shows that a high index of suspicion and a histopathology examination, including immunohistochemistry, will be necessary for an accurate diagnosis.

• 한국임상수의학회지
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• 제37권1호
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• pp.38-41
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• 2020

An 11-year-old castrated male miniature Schnauzer, weighing 8 kg, presented with multiple masses on the humeri. Grossly, the masses were separated from the surrounding soft tissues. Desmoplastic melanoma is an uncommon tumor in dogs. Fine-needle aspiration cytology showed large spindle-shaped cells with a high nuclear-cytoplasmic ratio. Histopathology showed interlacing bundles of fibroblast-like cells, highly pleomorphic multinuclear cells, occasional atypical spindle cells separated by fibrocollagenous stroma, and lymphocyte aggregates admixed with abundant collagen. The tumors were diffusely positive for S100a, a strong marker for desmoplastic melanoma, and negative for desmin. Herein, we report the pathobiology of a rare, uncommon variant of melanoma in a dog.

• 한국수의병리학회:학술대회논문집
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• 한국수의병리학회 2000년도 추계학술대회 및 정기총회
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• pp.25-25
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• 2000

N. caninum infections have been associated with neonatal paresis as well as abortion around the world. Bovine abortion induced by N. caninum was first reported in 1997 in Korea. Diagnosis of N. caninum infection is usually based on histopathology and immunohistochemical detection of organism. However, often the tissues having lesion suggestive of N. caninum infection were negative on immunohistochemistry. (omitted)

• Journal of Veterinary Science
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• 제25권5호
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• pp.61.1-61.13
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• 2024

Importance: Ovine pulmonary adenomatosis (OPA) and maedi-visna disease (MVD) are chronic and progressive infectious diseases in sheep caused by Jaagsiekte sheep retrovirus (JSRV) and maedi-visna virus (MVV), respectively. Objective: To investigate the pathological changes and conduct viral gene analysis of OPA and MVD co-occurrence in Inner Mongolia, China. Methods: Using gross pathology, histopathology, immunohistochemistry, ultrastructural pathology, PCR, and sequence analysis, we investigated the concurrent infection of JSRV and MVV in 319 Dorper rams slaughtered in a private slaughterhouse in Inner Mongolia, in 2022. Results: Of the 319 rams included, 3 showed concurrent JSRV and MVV infection. Gross lung pathology showed diffuse enlargement, consolidation, and greyish-white miliary nodules on the lung surface; the trachea was filled with a white foamy fluid; hilar and mediastinal lymph nodes were significantly enlarged. Histopathology results revealed typical OPA and MVD lesions in the lung tissue. Immunohistochemical results were positive for JSRV envelope protein (Env) in the tumor cells and MVV CA in alveolar macrophages. Transmission electron microscopy showed several virions and autophagosomes in the lung tissue, severely damaged mitochondria, and the induced mitophagy. Nucleotide sequences obtained for JSRV env and MVV gag showed the highest homology with the Inner Mongolian strains of JSRV env (JQ837489) and MVV gag (MW248464). Conclusions and Relevance: Our study confirmed that OPA and MVD co-occurrence and identified the pathological changes in Inner Mongolia, China, thereby providing references for the identification of concurrent JSRV and MVV infections.