• 약학회지
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• 제4권1호
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• pp.1-7
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• 1959

I) Bupleurum $\circled1$ In ooder to study on the histological review, we have devided into two types of all Bupleurum species of Korean products 1) Rhizoma type 2) root type and we selected one, B. leveilei (Jae-Ju-Do) and B. latislimum (Ul-Nung-Do) is Compared with each others as shown in figures. II) Gentiana Uchiyamana Nakai We have studied about the classifical seats of this Gentiana Uchiyamana Nakai. and compared with Gentiana sebra Bunge. III) Lysimachia Barystachys Bunge Also has been studied about the calssifical, echological steats, and we now decided the relation of this all species each others.

• 수산해양교육연구
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• 제26권2호
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• pp.245-256
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• 2014

잉어, Cyprinus carpio와 동자개, Pseudobagrus fulvidraco의 상악 하악 수염을 조직학적으로 조사하였다. 동자개의 수염은 연골성 증축(axial rod of cartilage), 신경섬유다발(bundle of nerve fiber), 표피(epidermis), 평활근 층(smooth muscle layer) 및 미뢰(taste bud)로 구성되었으며, 잉어의 수염은 표피, 신경섬유다발, 혈관(blood vessel) 및 미뢰로 구성되었다. 수염 길이에서 잉어는 상악 바깥쪽 수염(second maxillary barbel)이 상악 안쪽 수염(first maxillary barbel) 보다 길게 나타났으며, 동자개는 하악 안쪽(inner mandibular barbel), 상악 위쪽(upper maxillary barbel), 하악 바깥쪽(outer mandibular barbel), 상악 아래쪽(lower maxillary barbel) 순으로 길게 나타났다(P<0.05). 미뢰의 수를 고려하였을 때, 동자개와 잉어간의 미각에 대한 유의적 차이가 없었다(P>0.05). 아울러, 두 어종의 모든 수염에서 수염 상부의 미뢰 수가 하부의 미뢰 수 보다 높게 나타났다(P<0.05). 본 연구 결과, 동자개의 수염은 딱딱하며 굴절성인 수염(flexible and stiff type)이었으며 잉어의 수염은 연하고 유연한 수염(tender and yielding type)으로 파악되었다.

• Journal of Chest Surgery
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• 제27권9호
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• pp.785-792
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• 1994

In this study, the authors analyzed the prognostic value of four clinical variables[age and sex of patients, association with myasthenia gravis and clinical stage] and histological type in 30 consecutive patients with thymoma, histologically classified as cortical[10],medullary[5] and mixed[15]type according to Marino and Muller-Hermelink classification. There were significant differences between the histological types in the frequency of the different tumor stages and myasthenia gravis and prognosis.Most of the cortical thymomas were at stage III and all of the medullary and most of the mixed tumors at stage I or II.Myasthenia gravis occurred more commonly in patients with cortical[30%] and mixed thymoma[60%] than in patients with medullary thymoma[10%]. Follow-up was conducted in 30 patients,with follow-up range from 3 months to 120 months[mean,47.3months]. 5 year actuarial survival was 100% for medullary thymoma, 73% for mixed thymoma, and 47% for cortical thymoma.The overall survival curve shows that 87.6% of the patients are alive at 2 years and 72.8% at 5 years. And 7 patients was dead during follow-up periods.By Kaplan-Meier technique, we found that the patients who had myasthenia gravis had better prognosis[P<0.05]. Medullary thymoma is a comparatively rare, benign tumor, and usually not associated with myasthenia gravis. Cortical thymoma must be regarded as malignant. Mixed thymoma is intermediate in its behavior between medullary and cortical thymoma. But these tumors should be considered potentially malignant despite of presence as stage I of II disease. Also, the patients with stageI,II had good prognosis and the patients with total resection had good prognosis[P<0.05].

• Asian Pacific Journal of Cancer Prevention
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• 제15권22호
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• pp.9603-9606
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• 2014

Background: Endometrial cancer is the fourth most common cancer among women in developed countries. Patients with endometrial cancer may benefit from systemic chemotherapy alone or in combination with targeted therapies if the disease is clinically diagnosed prior to spread and metastasis to other organs. The aim of this study was to evaluate the prognostic role of p53 polymorphism and its correlation with tumor grade in human uterine endometrial carcinomas. Materials and Methods: A total of 75 patients with endometrial carcinomas were studied for possible mutations in exon 4 of the p53 gene using polymerase chain reaction and restricting fragment length polymorphism techniques and sequencing. Results: In recent study, The rate of homozygote genotype of pro/pro or Arg/Arg in high grade group was higher than in comparison with low grade one. In addition samples that were undigested in RFLP, showed mutation in exone 4. Conclusions: Our findings showed that high grade endometrial carcinomas are highly associated with TP53 polymorphisms in comparison with low grades.

• 한국동물학회지
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• 제35권3호
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• pp.295-307
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• 1992

In order to study the differentiation of the epithelial cells during the development of fetal rat lung tissue, histological changeB in organotypic culture and in vivo were examined. Light microscopy and scanning electron microscopy were used to analvre the histological change in rat lung from the 15th nary of gestation to the 111th nary after birth. In organotypic culture system, the pulmonary epithelial cell differentiation was studied by scanning electron microscopy. The results obtained from this study were as follows. 1. During deveiopment of lung, the glandular stage lasted from the Isth day to the lsth naut of gestation; the canalicular stage from the 17th nay to the 19th naut of gestation; the saccuiar stage from 20th nary to the birth. Alveolar stage was observed at the 3rd nary of postnatal rat lung. 2. In organotvpic culture of fetal rat lung cells organized alveolar-like structures resembling those of in uiuo state were observed on the gelatin matrix. In contrast with in vivo state, fetal lung cells formed group of type ll pneumocytes predominently along the contours of the matrix. These cells have large apical surface, short microvilli and secreted materials which may be sunactant. These results suggested that an orsanotypic culture retaining epithelial- -mesenchvmal relationships is appropriate culture model to study the pulmonary epithelial cell (especially type ll pneumocvte) differentation.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제38권1호
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• pp.2-8
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• 2012

Introduction: Auto-tooth bone graft material consists of 55% inorganic hydroxyapatite (HA) and 45% organic substances. Inorganic HA possesses properties of bone in terms of the combining and dissociating of calcium and phosphate. The organic substances include bone morphogenetic protein and proteins which have osteoinduction capacity, as well as the type I collagen identical to that found in alveolar bone. Auto-tooth bone graft material is useful as it supports excellent bone regeneration capacity and minimizes the possibility of foreign body reaction,genetic diseases and disease transmission. Materials and Methods: Implant placement combined with osteoinductive regeneration,preservation of extraction socket, maxillary sinus augmentation, and ridge augmentation using block type,powder type, and block+powder type autobone graft materialwere performed for 250 patients with alveolar bone defect and who visited the Department of Oral and Maxillofacial Surgery, College of Dentistry, Dankook University from September 2009 to August 2011. Results: Clinical assessment: Among the 250 patients of auto-tooth bone graft, clinical assessment was performed for 133 cases of implant placement. The average initial stabilization of placed implants was 74 implant stability quotient (ISQ). Radiological assessment: The average loss of crestal bone in the mandible as measured 6 months on the average after the application of prosthesis load was 0.29 mm, ranging from 0 mm to 3.0 mm. Histological assessment: In the histological assessment, formation of new bone, densified lamellated bone, trabecular bones, osteoblast, and planting fixtures were investigated. Conclusion: Based on these results, we concluded that auto-tooth bone graft material should be researched further as a good bone graft material with osteoconduction and osteoinduction capacities to replace autogenous bone, which has many limitations.

• 동의생리병리학회지
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• 제19권3호
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• pp.618-622
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• 2005

Articular cartilage is an important target for studying the arthritic diseases. To verify the therapeutic effects of bee venom herb-acupuncture in vivo, 3${\mu}$l of diluted solution of bee venom for herb-acupuncture were injected into articular cavity once a day during 3 months after making full-thickness defects in rat articular cartilage. Histological examination and immunohistochemistry indicated that the chondrocyte-like tissue was formed during the repair process of cartilage injury, and the expression of a cartilage-specific protein, collagen type II, were significantly activated. It means that the expression of the gene encoding type I collagen was down-regulated, whereas those of collagen type II were up-regulated. Histological examination by hematoxylin-eosin staining indicated that the cells regained their original round morphology. In addition, a homogeneous distribution of articular cartilage extracellular matrices was detected around the cells. These results suggested that bee venom herb-acupuncture was very effective on the recovery of articular chondrocyte phenotype.

• Asian-Australasian Journal of Animal Sciences
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• 제31권10호
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• pp.1565-1574
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• 2018

Objective: The aim of the present study was to identify genetic variants based on RNA-seq data, obtained via transcriptome sequencing of muscle tissue of pigs differing in muscle histological structure, and to verify the variants' effect on histological microstructure and production traits in a larger pig population. Methods: RNA-seq data was used to identify the panel of single nucleotide polymorphisms (SNPs) significantly related with percentage and diameter of each fiber type (I, IIA, IIB). Detected polymorphisms were mapped to quantitative trait loci (QTLs) regions. Next, the association study was performed on 944 animals representing five breeds (Landrace, Large White, Pietrain, Duroc, and native Puławska breed) in order to evaluate the relationship of selected SNPs and histological characteristics, meat quality and carcasses traits. Results: Mapping of detected genetic variants to QTL regions showed that chromosome 14 was the most overrepresented with the identification of four QTLs related to percentage of fiber types I and IIA. The association study performed on a 293 longissimus muscle samples confirmed a significant positive effect of transforming acidic coiled-coil-containing protein 2 (TACC2) polymorphisms on fiber diameter, while SNP within forkhead box O1 (FOXO1) locus was associated with decrease of diameter of fiber types IIA and IIB. Moreover, subsequent general linear model analysis showed significant relationship of FOXO1, delta 4-desaturase, sphingolipid 1 (DEGS1), and troponin T2 (TNNT2) genes with loin 'eye' area, FOXO1 with loin weight, as well as FOXO1 and TACC2 with lean meat percentage. Furthermore, the intramuscular fat content was positively associated (p<0.01) with occurrence of polymorphisms within DEGS1, TNNT2 genes and negatively with occurrence of TACC2 polymorphism. Conclusion: This study's results indicate that the SNP calling analysis based on RNA-seq data can be used to search candidate genes and establish the genetic basis of phenotypic traits. The presented results can be used for future studies evaluating the use of selected SNPs as genetic markers related to muscle histological profile and production traits in pig breeding.

• 대한임상검사과학회지
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• 제50권3호
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• pp.217-224
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• 2018

매선요법은 다른 시술에 비해 비침습적이고, 인체 내에서 생분해가 일어나는 물질로 제작되기 때문에 성형수술에서 각광받고 있다. 상업적으로 이용가능한 매선의 종류로는 poly-L-lactic acid (PLLA), polycarprolactone (PCL), polydioxanone (PDO) 등이 있으나, 매선으로 사용되는 생분해성 물질들의 종류에 따른 차이점이나, 콜라겐을 형성하는데 있어서 매선의 어떤 부분이 결정적으로 작용하는지에 대한 연구는 잘 알려져 있지 않다. 따라서 이 연구에서는 현재 판매되고 있는 3가지 생분해성 물질(PLLA, PCL, PDO)로 만들어진 매선을 동물모델에서 그 효과를 비교하고, 매선의 콜라겐생성능에 미치는 영향 인자가 무엇인지를 분석하였다. 매선을 랫드의 등에 삽입하여 2주, 4주, 8주, 12주동안 경과를 관찰하고 특수염색을 통해 콜라겐의 생성 정도를 비교하였다. 매선에 의한 조직학적 변화를 관찰하기 위해서, 조직검체는 H&E, Masson's trichrome, Herovici's collagen stain이 진행되었다. 그 결과 3가지 모두 다 동일한 시기에 비슷한 양의 신생콜라겐을 형성하는 것을 관찰할 수 있었고, 3가지 매선 모두 type 3의 콜라겐을 형성하는 것을 확인할 수 있었다. 또한 생성되는 콜라겐의 양은 매선이 피부조직에 닿는 면적이 크면 클수록 증가하는 것을 관찰할 수 있어 한 번의 시술만으로도 많은 양의 매선이 들어갈 수 있는 PDO cavern타입의 매선이 가장 많은 콜라겐을 형성하는 것을 확인할 수 있었다.

• 한국패류학회지
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• 제11권2호
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• pp.135-145
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• 1995

A morphological andk histochimical study on the amterior tintacular antenna of Korean sulg, Incilaria fruhstorferi was conducted under the light microscopic observations. The histological sturctures of the antenna were apparently divided into three parts such as the epithelium, the connective tissues and the muscular layers. The cells forming the antenna were classified into several types on the basis of their morphological and histochemical characteristics. The simple columnar epithelium cotering the whole antenna was composed of supporting cells, sensory neurons and type-a clear cells. The connective tissue was consisted of dispersed large cells, type-b clear cells and 7 types of secretory cills such as type-A, type-B, type-F, thpe-G, type-H, type-J and type-K. The large cells found in the form of group situated only in the stalk of the antenna. The large cells possessed relatively small nuclei as compared with their cytoplasm. The cytoplasm positively reacted upon alcian blue, and the nucleus was PASpositive. The type-a and type-b clear cells which were irregular in shape showed no evident reaction against various stains employed in the present study. The secrtory cells were observed mainly in the connective tissues and in the muscular layers. Histochemical components of the type-A, type-B and K were identified as acid mucopolysaccharides and those of type-F and H were neutral mucopolysaccharides. The muscular layders supporting the epithelium possessed the type-B and F secretory cells which were also observed in the connective tissues.