• 한국발생생물학회지:발생과생식
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• 제18권1호
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• pp.51-56
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• 2014

This study describes the developmental process of gonads in chameleon goby, Tridentiger trigonocephalus from the stage of hatching to 100 days after hatching (DAH). Based on histological observation, the primordial germ cells were observed in mesentery between mesonephric duct and gut at 15 DAH (total length, TL: $6.8{\pm}0.2mm$). At 20 DAH (TL: $7.9{\pm}0.1mm$), the primordial gonad began to protrude into peritoneal cavity and developed between mesonephric duct and gut. Initial ovarian differentiation was identified by the presence of ovarian cavity and oogonia in the gonads at 55 DAH (TL: $21.1{\pm}1.3mm$). Testicular differentiation started at 65 DAH (TL: $23.7{\pm}0.9mm$) with appearance of spermatogonial cells in the gonads. These findings indicate that sex differentiation in T. trigonocephalus occurs earlier in females than males, suggesting that this species can be classified as an undifferentiated gonochorist.

• 대한수의학회지
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• 제26권2호
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• pp.195-199
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• 1986

The papillary tubercles (PTs) developed on the splenic capsule of normal Landrace pigs were collected and their histological structures were observed with light microscope. The results were summarized as follows : 1. The external features of the PTs were smooth spherical or oval form protruded on the splenic capsules. On cross section of PTs, the shapes were predominantly round or elliptical single follicular form, and were often multifollicular and irregular form in some PTs. 2. The PTs were interposed into the splenic capsule. Therefore the peripheral boundary of PT was consisted of splenic capsular tissue and this tissue was covered with mesothelium, The basal tissues of PT were consisted of thick connective tissue and smooth muscle of splenic capsule, and capsular foramen for transport tract between splenic parenchyma and the PT was found at the center of the basal boundary of PT. 3. The basal region of PT was composed of parenchyma and this tissue was the splenic red pulp but the central and peripheral regions of PT contained much more erythrocytes than in the splenic parenchymae. 4. The splenic parenchymae adjoining to PT contained more erythrocytes than in other splenic parenchymal regions and parallel fixed cells directed to the capsular foramen.

• 한국패류학회지
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• 제27권4호
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• pp.283-289
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• 2011

염분변화에 따른 북방전복의 생리적 반응을 조사하기 위하여 고염분 및 저염분의 농도에 따라 생존율, 삼투질농도, 대사율 및 조직학적 반응을 분석하였다. 염분변화에 따른 북방전복의 생존율은 37 psu에서는 100%였다. 반면 19.8 psu에서 노출 6일째, 12.8 psu 이하에서는 노출 24시간 이내 모두 사망하여 북방전복의 7-days $LS_{50}$은 24.9 psu (20.1-28.2 psu)를 나타내었다. 염분변화에 따른 호흡률은 대조구에 비해 감소하였으며, 반면 암모니아질소 배설률은 증가하였다. 삼투질농도는 염분 26.8 psu이상에서는 1시간 이내 순치하였다. 북방전복 발근육의 조직학적 반응은 염분감소에 따라 혈림프동의 확장, 상피세포의 괴사 및 식세포 증가 등의 현상이 관찰되었다.

• Asian-Australasian Journal of Animal Sciences
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• 제25권6호
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• pp.800-805
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• 2012

Rice straw is an important roughage resource for ruminants in many rice-producing countries. In this study, a rice brittle mutant (BM, mutation in OsCesA4, encoding cellulose synthase) and its wild type (WT) were employed to investigate the effects of a cellulose synthase gene mutation on rice straw morphological fractions, chemical composition, stem histological structure and in situ digestibility. The morphological fractions investigation showed that BM had a higher leaf sheath proportion (43.70% vs 38.21%, p<0.01) and a lower leaf blade proportion (25.21% vs 32.14%, p<0.01) than WT. Chemical composition analysis showed that BM rice straw was significantly (p<0.01) higher in CP (crude protein), hemicellulose and acid insoluble ash (AIA) contents, but lower in dry matter (DM), acid detergent fiber (ADFom) and cellulose contents when compared to WT. No significant difference (p>0.05) was detected in neutral detergent fiber (NDFom) and ADL contents for both strains. Histological structure observation indicated that BM stems had fewer sclerenchyma cells and a thinner sclerenchyma cell wall than WT. The results of in situ digestion showed that BM had higher DM, NDFom, cellulose and hemicellulose disappearance at 24 or 48 h of incubation (p<0.05). The effective digestibility of BM rice straw DM and NDFom was greater than that of WT (31.4% vs 26.7% for DM, 29.1% vs 24.3% for NDFom, p<0.05), but the rate of digestion of the slowly digested fraction of BM rice straw DM and NDF was decreased. These results indicated that the mutation in the cellulose synthase gene could improve the nutritive value of rice straw for ruminants.

• 대한약침학회지
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• 제1권1호
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• pp.35-52
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• 1997

In order to investigate experimentally that Bovis Calculus, Ursi Feli Aqua-acupuncture(BUA) have an effect on Adjuvant Arthritis in rats, the author inserted BUA at points corresponding with Chok-samni(ST36) and T'aegye(KI3), and observed an inhibitory rate of edema and pain, variations of White blood cell(WBC), Red blood cell(RBC), Hemoglobin(Hb) in blood. The author also observed the histological changes of joint tissue. The results were as follows : 1. The BUA group during the 6th and 9th were decreased with statistical significance in inhibitory rate of paw edema as compared with the control group. 2. The BUA group during the 3rd and 9th day were decreased with statistical significance in inhibitory rate of pain as compared with the control group. 3. The BUA group during the 3rd, 6th and 9th day were decreased with statistical significance in blood WBC as compared with the control group. The blood RBC and Hb didn't have statistical significance. 4. According to the histological studies, the synovial cells were necrotized at the 3rd, 6th and 9th day control group, but some synovial cells were necrotized at the 3rd day BUA group. The synovial cells of the the 6th and 9th day BUA group were recovered more than that of the 3th day group.

• Radiation Oncology Journal
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• 제8권1호
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• pp.17-27
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• 1990

온열요법이 X-선조사에 미치는 영향을 연구하기 위하여 저자는 100 watt, 2450 MHz의 마이크로파 온열기구를 제작하여 백서 60마리를 대상으로 고환에 온열요법 ($43^{\circ}C$, 15분), X-선 조사($2\;Gy\~8\;Gy$ 단독 혹은 병행하여 조직학적 변화를 관찰하여 다음과 같은 결론을 얻었다. 1. 2450 MHz 마이크로파 온열요법 단독 시행하여 조직학적 변화는 관찰되지 않았다. 2. X-선 조사후 초기변화는 정조세포의 변성을 2 Gy에서 나타나기 시작하였으며 괴사는 6 Gy 후 심하게 보였고 8 Gy 후 완전소실되었다. 3. X-선 조사와 온열요법 병행하여 2 Gy후 정조세포의 괴사와 소실이 나타나기 시작하였으며 6 Gy 후 완전소실되어 열증강율은 1.3 이었다. 4. X-선조사, 온열요법 단독 및 병행후 추적기간 15일군과 30일군의 차이는 관찰할 수 없었다.

• 환경생물
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• 제35권4호
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• pp.468-475
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• 2017

Kudoa septempunctata에 대한 PCR 검사에서 양성으로 확인된 개체를 조직 내 포자가 확인된 개체(3마리)와 포자가 확인되지 않은 개체(4마리)를 선별하여 순치 후 혈액과 조직검사를 실시하였다. 혈액에 대하여 PCR 검사를 실시하여 포자가 명확하게 확인되었던 2개체에서는 음성이 확인되었으며, 다른 5개체는 감도는 각각 차이는 있었지만 양성으로 확인되었다. 포자가 확인된 개체의 각 장기(간, 비장, 신장, 심장, 위, 소화관, 근육)에 대하여 PCR 검사를 실시한 결과, 심장과 근육에서 명확한 양성이 나타났다. 근육조직의 H&E 염색과 ISH를 실시하여 K. septempunctata의 감염으로 근섬유에 괴사가 발생하고, 이 부위에 포자의 슈도시스트가 형성되는 것을 확인하였으나 심한 염증반응을 유도하지는 않는 것이 확인되었다. 이후 혈액에 대한 더욱 면밀한 조사가 필요할 것으로 보이며, 연구내용은 넙치양식에서의 쿠도아감염증의 예방과 구제를 위한 기반자료가 될 것으로 기대된다.

• Medical Lasers
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• 제10권2호
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• pp.96-105
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• 2021

Background and Objectives Skin aging is reportedly associated with regulation in collagen and elastin synthesis. This study investigated the potential of combining light-emitting diode (LED) treatments using a 630-nm and 850-nm LED with simultaneous microcurrent application. Materials and Methods The dorsal skin of female pigs was treated with a home-use device. We examined the treatment effects using photography, thermocamera, microscopic pathology, and histological examination to determine the mechanism of action, efficacy, and safety of the procedure. A histological observation was performed using hematoxylin and eosin, Masson's trichrome, Victoria blue, and immunohistochemical staining. We also used the Sircol soluble collagen and elastin assay kit to measure the amounts of collagen and elastin in the porcine back skin tissue after 2 and 6 weeks. Results Evaluation by visual inspection and devices showed no skin damage or heat-induced injury at the treatment site. Histological staining revealed that accurate treatment of the targeted dermis layer effectively enhanced collagen and elastin deposition. Collagen type I, a protein defined by immunohistochemical staining, was overexpressed in the early stages of weeks 2 and 6. Combined therapy findings showed the superior capability of the 630-nm and 850-nm LED procedures to induce collagen; in contrast, elastin induction was more pronounced after microcurrent treatments. Conclusion The home-use LED device, comprising a combination of 630-nm and 850-nm LEDs and microcurrent, is safe and can be used as an adjunctive treatment for self-administered facial rejuvenation.

• Journal of Acupuncture Research
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• 제23권1호
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• pp.105-120
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• 2006

To investigate anti-cancer effects of wild ginseng herbal acupuncture and mitigation of anti-cancer drug when taken concurrently, cancer cells from B16/F10 melanoma were injected intraperitoneally in C57BL/6. After inducing cancer, anti-cancer effects and mitigation of reproductive toxicity of Doxorubicin were evaluated. 1. For changes in weight, Doxorubicin treated group showed significant decrease, and administration of wild ginseng herbal acupuncture didn't cause any weight change. 2. Volume of tumor was significantly reduced in Doxorubicin teated group. Wild ginseng herbal acupuncture groups showed slight decrease but insignificant compared to the control group. 3. For hematological evaluation, Doxorubicin only group's reticulocytes were significantly decreased compared to the control group, and Platelet Count was significantly increased. Wild ginseng herbal acupuncture group showed significant increase of Neutrophils and significant decrease of Lymphocytes compared to the control group. 4. For histological evaluation of the tumor, necrosis occurred in a wide range in the Doxorubicin treated group. Wild ginseng herbal acupuncture didn't cause much histological changes. 5. For histological evaluation of the testis, seminiferous tubules of the control group suffered severe damage on epithelial cells. When wild ginseng herbal acupuncture was administered concurrently, damage on the seminiferous tubules was significantly inhibited compared to the Doxorubicin only group. 6. Diameter of seminiferous tubules and spermatogonia count were insignificant between the experiment groups. 7. For BrdU positive reaction of testicle tissue, Doxorubicin only group failed to show any reaction of spermatogonia, but spermatocytes and spermatids showed slight positive reaction. When wild ginseng herbal acupuncture was treated concurrently, much greater positive reaction was made but similar to that of the control and normal groups. 8. For observation of changes in BrdU spermatogonia count of the testicle tissue, Doxorubicin only group didn't show any positive reaction, and relative increase was shown in the group with concurrent administration of wild ginseng herbal acupuncture. 9. For observation of TUNEL positive reaction cells of the testicle tissue, no significant changes were witnessed in all the experiment groups.

• Journal of Oral Medicine and Pain
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• 제19권1호
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• pp.125-136
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• 1994

Observation of elastic fiber's change of mouse TMJ due to several round factor, principally external stimulations, their influence on the TMJ structure's change and the analize of the consecutive evolution of the disease in most important. So, the author believe that the factor of TMJ feature is the elastic feature's change and it's the principal factor of the TMJ disease. For observation of the increase and disposition of elastic fiber that to regulate the elastic feature of tissue and allow it existence. For this propose, observation with histologic methods on 20mouse ICR of 3 days, 1 week, 2 weeks, 3 weeks and 4 weeks. The results were as follow : 1. In the early stage, the condyle of TMJ is originated from cartilage mass, and it's calcification is endochondral. 2. In the early stage, the disc is relatively thin and immature, but in the later stage the fiber is dense and the disposition is most functional. 3. Observation of the early stage, the elastic fiber is a thin fiber that to across antero- posterior direction, but in the later stage elastic fiber are developed, the disposition that in the early stage was perpendicular to articular surface, now in parallel. 4. The elastic fiber was observated most clearly in the retrodiscal tissue. 5. In conclusion, the elastic fiber is observed like a thin fiber 1 week from born, but the fiber to increase the weight and it dispose functionally, and 4 week from born, it can realize the normal function.