• 한국임상수의학회지
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• 제30권4호
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• pp.247-252
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• 2013

We compared wound healing with $CO_2$ laser incision and scalpel incision by measuring the extent of bleeding, the ease of incision, time, degrees of adhesion and histological observation in pig's stomach. Eight healthy pigs were used. Two symmetrical incisions were made in ventral aspect of the stomach between the greater curvature and lesser curvature were made with scalpel and 2 mm spot diameter $CO_2$ laser (8W, continuous wave) in eight pigs. And then each wound was closed with absorbable suture in a two-layer inverting seromuscular pattern. At 7 and 14 days after initial wounding, each wound was taken for histological observation. On surgery, the extent of bleeding, the ease of incision and incision time showed significant differences between the two groups. The $CO_2$ laser provided better hemostasis (p < 0.01) and smaller postoperative adhesion compared with the scalpel. However, the scalpel produced faster speed of incision and was easier to handle than the $CO_2$ laser group (p < 0.01). There was no considerable difference between the two groups in histological observation.

• Imaging Science in Dentistry
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• 제50권3호
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• pp.183-192
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• 2020

Purpose: This study was performed to assess the accuracy of preoperative cone-beam computed tomography (CBCT), when justified for other reasons, in locating the apical foramen and establishing the working length. Materials and Methods: Six electronic databases were searched for studies on this subject. All studies, of any type, were included if they compared measurements of working length with preoperative CBCT to measurements using an electronic apex locator (EAL) or histological reference standard. Due to the high levels of heterogeneity, an inverse-variance random-effects model was chosen, and weighted mean differences were obtained with 95% confidence intervals and P values. Results: Nine studies were included. Compared to a histological reference standard, CBCT indicated that the apical foramen was on average 0.40 mm coronal of its histological position, with a mean absolute difference of 0.48 mm. Comparisons were also performed to an EAL reference standard, but the conclusions could not be considered robust due to high levels of heterogeneity in the results. Conclusion: A low level of evidence is produced suggesting that preoperative CBCT shows the apical foramen to be on average 0.40 mm coronal to its histological position, with a mean absolute difference of 0.48 mm.

• 한국전문물리치료학회지
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• 제16권2호
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• pp.41-49
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• 2009

This study was designed to investigate the effects of treadmill exercise of low-intensity and moderate- intensity on the functional recovery and histological change in spinal cord injury (SCI) rats. SCI was induced by the spinal cord impactor dropped after laminectomy. Experimental groups were divided into the Group I (normal control), Group II (non-treatment after SCI induction), Group III (low-intensity treadmill exercise after SCI induction), Group IV (moderate-intensity treadmill exercise after SCI induction). After operation, rats were tested at modified Tarlov scale at 2 days with divided into 4 groups, and motor behavior test (BBB locomotor rating scale, Grid walk test) was examined at 3, 7, 14, and 21 days. For the observation of damage change and size of the organized surface in spinal cord, histopathological studies were performed at 21 days by H & E, and BDNF(brain-derived neutrophic factor) & Trk-b immunohistochemistry studies were performed at 1, 3, 7, 14, 21 days. According to the results, treadmill exercise can play a role in facilitating recovery of locomotion following spinal cord injury. Specially, moderate-intensity treadmill exercise after SCI induction was most improvement in functional recovery and histological change.

• Environmental Analysis Health and Toxicology
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• 제21권1호
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• pp.35-43
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• 2006

Reproduction and organ structure of the pale chub, Zacco platypus in Isa stream were investigated by means of histological methods. The results of the study confirmed reproductive abnormality and histopathological features in the pale chub. The gonadosomatic index (GSI) of the fish showed two peak in April and August. In summer season, GSI of the male was about two times of the female's GSI. Monthly variation of the gonadal development was very irregular. From the histological analysis of the organ structure, epidermal atrophy, necrosis and hyperplasia of pigment cell were observed in the skin. Epithelial layer lifting and clubbing of the lamella and bifurcation of the filament were observed in the gill. Also histological changes as congestion, cytoplasmic degeneration of hepatic cell, degeneration of bile duct, glomerular dilatation, degeneration of renal tubule and pycnosis of interstitial cell were identified in the liver and kidney, respectively.

• Fisheries and Aquatic Sciences
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• 제21권1호
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• pp.2.1-2.8
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• 2018

This study evaluated hemato-histological changes of black porgy in recirculating aquaculture systems (RAS) with three different ozone doses (no ozone, 20 g, and $40g\;ozone/kg\;feed\;day^{-1}$). During the 44-day study, black porgy did not show significant behavior changes or mortalities in both the ozonated systems displaying average total residual oxidants concentrations of 0.12 and 0.25 mg/L. There were no differences in growth and blood parameters among the systems. However, histological alterations on gills and livers were observed in both the treatment systems. In the higher ozone dose, signs of cellular damage were more apparent. Although the ozone doses did not manifest a serious adverse effect on growth and hematological observations in this short-term study, an ozone dose should not exceed $20g\;ozone/kg\;feed\;day^{-1}$ for black porgy based on the histological result. In order to use ozone in a seawater RAS, further studies will be needed to evaluate long-term effects of total residual oxidants.

• 수산해양교육연구
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• 제28권4호
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• pp.1068-1075
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• 2016

The suitable temperature and salinity for the long-distance transportation for the rockfish were investigated by assessing survival rate, metabolism, histological change. All experimental groups showed survival rates of 100%. Daily Oxygen consumption rhythm was decreased during nights and increased during days. Average oxygen consumption was significantly decreased as temperature and salinity were decreased. Ammonia excretion was significantly increased as temperature and salinity were decreased. Histological changes were observed in the skin and gill of the rockfish exposed to 10 psu under all the temperature conditions, with larger changes at $4^{\circ}C$. Further, nucleus deformation and uniformity in the cytoplasm were also observed.

• 동의생리병리학회지
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• 제20권6호
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• pp.1543-1547
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• 2006

The aim of this experiment was to observe and analysis clinical effect of the 'Sexiang shuhuo Jing' on histological change for 14days after skeletal muscle injury in rats. The gastrocnemius muscles of rats were damaged by electromechanical and serial cryosections of the damaged muscle were prepared at 1, 5, 10, 14 days after injury. Muscle sample of the both control and 'Sexiang Shuhuo Jing' treated group were prepared for histological analysis by optical microscope and electron microscopy. 'Sexiang Shuhuo Jing' treatment group's skeletal muscle recovery was much more faster than control group. After 5 day's 'Sexiang Shuhuo Jing' treatment group's basically recovery normal structure of muscle fiber. After 14 day's control group's damaged muscle were basically recovery structure of muscle fiber but still has some factor of pathological impression but in Sexiang Shuhuo Jing treatment group's can't be found that.

• Journal of Veterinary Science
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• 제19권6호
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• pp.725-734
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• 2018

Ovaries of 21 bitches presented with gynecopathies were surgically removed and histologically examined. Standard histological, as well as immunohistochemical, classification of 193 cystic structures resulted in the classification of 72 cysts of subsurface epithelial structures (SES), 61 follicular cysts (FCs), 38 cystic rete ovarii (CRO), 13 lutein cysts (LCs), and 9 non-classifiable cysts (NCCs). In addition to the histological classification, results were interpreted according to subject medical history, clinical examination outcome, and macroscopic observations during ovariohysterectomy. Dogs with ovarian cysts (OCs) and associated reproductive perturbations were mostly nulliparous, of large breed, and had an average of $9.5{\pm}3$ years. Prolonged or shortened inter-estrus intervals of past heats, however, seemed to be relatively low-risk factors for the development of OCs in dogs. Furthermore, we provide histological observations of a rarely seen canine LC including a degenerated oocyte in the central cavity.

• 대한본초학회지
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• 제32권3호
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• pp.79-87
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• 2017

Objectives : This study aimed to investigate the protective effects of an herbal mixture of Atractylodes macrocephala and Taraxacum spp. (ATC) on ulcerative colitis. We have previously screened traditional medicinal herbs to discover the effective candidate by the animal model. A. macrocephala and T. spp were identified as one of the effective herbs in the screening process. Methods : Experimental colitis was induced in male Balb/c mice by administering drinking water containing dextran sulfate sodium, which mimics the clinical and histological features of ulcerative colitis in human. ATC at doses of 30, 100 or 300 mg/kg were orally administered to mice twice per day for 10 consecutive days. To evaluate the damage from experimentla ulcerative colitis, body weight, colon length, disease activity index, myeloperoxidase and histological changes were measured and analyzed. Results : The administration of dextran sulfate sodium with drinking water resulted in markedly reduced colon length, severe body weight loss, increased levels of myeloperoxidase activity and histological damages in mice. ATC treatment significantly ameliorated the colon shortening, histological damage, body weight loss and disease activity index score in a dose-dependent manner. ATC also attenuated the colonic myeloperoxidase activity which reflects the severity and extent of inflammatory damage of colon. Conclusions : ATC exerts protective effects against inflammatory colonic structural damage induced by epithelial barrier integrity impairment. ATC also inhibits weight loss and related symptoms of UC which can be considered as the functional recovery of colon.

• 대한치과교정학회지
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• 제48권6호
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• pp.405-411
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• 2018

Objective: This study was performed to investigate the rate of tooth movement and histological characteristics of extraction sockets those were subjected to corticotomy. Methods: A split-mouth randomized controlled trial experiment was designed. Thirty-two adult, male Wistar rats were divided into 2 groups: healing extraction socket (H) and recent extraction socket (R); these groups were randomly classified into 4 subgroups (0/7/21/60 days). The first maxillary molar was extracted on 1 side and 2 months were allowed for complete bone healing; then, the corresponding molar was extracted on the other side and surgical intervention was performed at the mid-alveolar point of the first maxillary molar. Ten grams of continuous force was applied. The outcomes measured were rate of tooth movement, percentage of periodontal space and histological evaluation. The rate of tooth movement was calculated as the measured distance divided by the duration of molar movement. Histomorphometric evaluations were performed on the second and third maxillary molars. The Wilcoxon signed rank test was used to compare differences between the two groups. Results: There were no significant differences in the rates of tooth movement between H and R groups at any of the 4 time points. The histological appearance and percentage of periodontal space between the R and H groups also demonstrated no significant differences. Conclusions: The rates of orthodontic tooth movement into recent and healed socket sites did not differ between the groups. Histological analysis of tooth movement revealed regional acceleration during every time period.