• Journal of Veterinary Science
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• 제25권1호
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• pp.14.1-14.5
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• 2024

An adult female ringed seal died suddenly and was subsequently examined for diagnostic purposes. The animal's lungs demonstrated mild non-collapsibility and multifocal white to yellow patches. Histopathological examination revealed multifocal pulmonary histiocytosis. Alveoli were filled with numerous foamy macrophages cytoplasm and scattered multinucleated giant cells containing cholesterol clefts. The foamy cytoplasm of the macrophages stained with oil red O stain. Further, lipid droplets within the cytoplasm were detected by electron microscopy. To the author's knowledge, this is the first case report describing the histochemical staining and electron microscopic findings associated with endogenous lipid pneumonia in ringed seal.

• 한국동물학회지
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• 제29권2호
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• pp.141-158
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• 1986

흰쥐를 실험재료로 그 적혈구에서 glutathione peroxidase를 $(NH_4)_2SO_4$ 침전법, Sephadex filtration column, DEAE-sephadex column chromatogrgphy하여 순화시키고 이것의 성질을 연구하였으며, 간에서 이 효소의 작용부위를 조직화학적 처리를 하여 전자현미경으로 관찰한 결과는 다음과 같다. 1. Glutathione peroxidase는 약 33.5배 순화되었다. 2. Crude glutathione peroxidase의 최적온도는 $40^\\circC$이며, 반응 최적 pH는 7.5였다. 3. 이 효소는 $30^\\circC$에서 가장 안정되었으며 glutathione농도 변화에 대한 Km값은 8.5 mM, 최대 반응속도는 15.6 $\\mu$moles/min이었고, $H_2O_2$ 농도변화에 대한 Km값은 40 $\\mu$M이며, 최대 반응속도는 10.5 $\\mu$moles/min이었다. 4. 이 효소의 분자량은 약 90,000정도로 측정되었다. 5. 쥐의 간에서 이 효소의 활성부위는 microbody에 국한되며 간소염의 주변주에서 그 활성이 크게 나타났다. 6. 조직화학적 방법으로 나타난 반응산물은 직경 $2.0\\sim0.7 \\mum$ 정도의 원형으로 그 경계막은 뚜렷하지 않았다.

• 한국동물학회지
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• 제36권2호
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• pp.159-174
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• 1993

까치 살모사(AEkistrodon snatilis)의 활동기와 동면기에서 일반적인 혈액성상과 혈액내 가스함량의 변화 또한 위장관 점액세포에 대한 형태적 및 조직화학적 변화 양상을 규명 비교하고자 한다 활동기와 동면기에 까치 살모사의 혈액내 총이산화탄소량(TCO2). 이산화탄소부분압(PCO2), 산소압(PO2)과 pH는 활동기보다 동면기에 증가하였으며, 적혈구수, 혈색소, 적혈구 용적 백분율은 활동기보다 동면기에 약간 증가하는 경향이었다. 또한 백혈구수는 활동기보다 동면기에 뚜렷한 감소를 보였다 활동기와 동면기의 위점막에서는 현저한 차이는 없으나, 소장 점막은 동면기에 그 주름수가 감소하고 높이가 낮아지며 점액세포가 위축되었으며, 위 표면 점액세포는 활동기에 중성 점액질과 약간의 Sialomucin을 포함한 산성 점액질로 구성되었으나 동면기에는 중성 점액질과 Sialomucin이 약간 감소하였다 위점액경세포는 활동기에 중성 점액질만을 포함하고 있었으나. 동면기에는Sialomucin을 포함한 산성 점액질이 소량 검출되었다 소장 점액세포는 활동기에 산성점액질과 중성 점액질로 구성되었으며. 산성 점액질에는 Sulfomucin과 Sialomucirr이 포함되어 있었으나 동면기에는 Sulfomucin과 Sialomucin이 다소 감소하는 경향이었다 주사전자현미경 관찰을 통한 까치 살모사의 위장관 표면 상피세포의 미세구조적 변화는 각동면기 별로 차이를 인정할 수가 있었다. 위장관점 막 상피세포는 활동기에 비해 동면기에 상당한 구조적 위축현상을 보이는 경향을 알 수 있다.

• 대한수의학회지
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• 제33권4호
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• pp.633-647
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• 1993

NC strain mice were inoculated intraperitoneally with Dermatophoilus-like microorganism strain W254 isolated from the sulfur granules of the swine tonsils. The development of the resulting abscesses in NC strain mice was investigated at regular time intervals by means of histological, histochemical and electron microscopical methods. The results obtained were as follows ; 1. The inoculated bacteria have settled in the subserous site of the peritoneal organ and provoked cellular response to form neutrophil, macrophage and connective tissue layers at the time of one week after inoculation, and thereafter were found to have formed typical actinomycotic abscess two weeks after inoculation. 2. The persistant abscesses were appeared to reveal close interaction between higher activity of organism and tissue reaction and contained hyaloid bodies within or outside bacterial clumps. 3. The hyaline materials were appeared to be composed of protein rich in hydroxyphenyl group by histochemical examination. The materials were presumed to be produced by the interaction between bacterial activity and host cell reaction. 4. The bacteria in the abscesses showed the various developmental stages of coccoid body, mulberry-like body tuber-shaped body and filamentous body, and it was confirmed that the mesosomal development was related to septation of the bacterial cells.

• 한국동물위생학회지
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• 제29권4호
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• pp.507-511
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• 2006

Acanthomatous epulis is a malignant form of gingival tumor. This is a case of epulis affecting a four-year old, intact, female Maltese dog raised as indoor pet in Iksan city. Clinical examination showed an enlarged palpable mass along the rostral maxillary gingiva. Section of the excised mass showed epithelial overgrowth and expansion from the basal portions through the tissue stroma. Post-surgical radio-graph showed displacement of the first and second maxillary incisors. Histochemical staining showed strongly positive reaction to epithelial marker cytokeratin and moderately positive reaction to connective tissue marker vimentin. This case was diagnosed as a form of maxillary gingival acanthomatous epulis. Routine clinical oral examination is suggested.

• 한국식품과학회지
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• 제14권4호
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• pp.336-341
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• 1982

미역김의 제조와 이화학적 특성에 관한 계속 연구로 실시한 미역김의 조직화학적 특성 연구 결과는 다음과 같다. 미역잎은 표층, 피층, 속층으로 구성되어 있는데 미역김의 결합단면을 광학현미경으로 관찰한 결과 피층과 속층이 완전히 파괴되었고 표층세포끼리 결합되어 있었다. 미역김 절편을 $Na_2CO_3$용액 처리로 알진산을 추출한 후 광학현미경으로 검경한 결과 알진산이 추출된 결합부위는 periodic acid schiff reagent반응이 나타나지 않았으며, 한편 미역잎을 $Na_2CO_3$용액으로 미리 처리하여 알진산을 추출한 후에는 정상적인 미역김을 제조할 수 없었고 비정상적인 결합구조이었으므로 알진산이 미역김의 결합물질임을 확인할 수 있었다. 미역김의 결합부위를 전자현미경으로 관찰한 결과 알진산의 결합위치는 미역김의 표층세포막과 세포막사이임이 밝혀졌다. $Na_2CO_3$용액을 처리한 미역잎으로 제조한 비정상적인 미역김의 결합구조는 표층세포껍질로 인지되는 물질만이 서로 엉켜 있었다.

• 대한방사선기술학회지:방사선기술과학
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• 제24권2호
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• pp.19-22
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• 2001

The purpose of this study is to evaluate time course of signal enhancement on Gadomer-17 enhance MRI, and to correlate the size of enhanced area with that of the infarct area on 2'3'5'-triphenyl tetrazolium chloride(TTC) histochemical examination for the assessment of myocardial viability in reperfused Myocardial Infarction in a cat model. Tan cats(average weight: 3.8 kg) which had undergone 90 minutes of occlusion of the LAD followed by 90 minutes of reperfusion underwent MR T2-weighted imaging, and T1-weighted imaging, enhanced T1-weighted imaging. We used 1.5T Magneton Vision MRI system(Siemens, Erlangen, Germany). Signal intensities were measured in the enhanced and non-enhanced areas of enhanced T1-weighted imaging. and TTC histochemical staining the size of the abnormal signal area on each image was compared with that of the infarct area. Maximum enhancement was detected during a $40{\sim}60$ minute period with an average enhancement of $168{\pm}9.9%$ of normal myocardium. TTC staining revealed that the size of the high signal area on T2-weighted images and of the enhanced area on enhanced T1-weighted images was greater than that of the infarct area($T2=48.1%{\pm}3.7$, enhanced $T1=47.2%{\pm}2.6$, TTC $staining=38.7%{\pm}3.1$ ; p<0.05). In reperfused Myocardial Infarction in a cat model, enhanced MR imaging delineates reversibly and irreversibly damaged myocardium, with a strong enhancement and a broad temporal window. We may therefore expect that enhanced MR image is useful for demonstrating myocardial injury.

• 한국동물위생학회지
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• 제30권4호
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• pp.539-544
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• 2007

A 7-year-old intact female Golden Retriever was presented for examination. The dog had large irregular subcutaneous masses in the abdomen which were ruptured or encapsulated. Those were removed surgically. Histopathologically, the masses consisted of spindle cells that often formed distinct whorls around a central capillary. Immuno-histochemical analysis revealed that the neoplastic cells were strong diffuse cyto-plasmic immunolabelling for vimentin and focal immunoreactivity for smooth muscle actin, whereas not immunoreactive for cytokeratin, desmin, von Willebrand factor, glial fibrillary acidic protein, or S-100. The neoplastic cells ultrastructurally had processes attached by desmosome-like structures, swollen mitochondria and dilated rough endoplasmic reticulum. Based on the above results, this case was diagnosed as a canine hemangiopericytoma in the abdominal subcutis of a Golden Retriever.

• 대한치과의사협회지
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• 제12권5호
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• pp.313-316
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• 1974

The experimental female rats were ovariectomized and after 14day, they were injected intramuscullarly with estrogen of single dose, 2/100gm body weight. After hormone injection, the animals were sacrificed by ether inhalation, the salivary glands were removed for microscopic examination and the histochemical preparations were observed. The results were as followed; In the submaxillary gland of ovariectomized rats, the acinar cells have lesser PAS reactivity than control group, and-SH group reaction were slight. In the estrogen injected-ovariectomized rats, aciner cells and striated duct cells have high reactivities for PAS, and-SS and SH group reaction were more increased than control group.

• Advances in pediatric surgery
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• 제8권1호
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• pp.48-53
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• 2002

Diagnosing Hirschprungs disease (HD) is a clinical challenge to pediatric surgeons. The cardinal symptoms are failure of passage of meconium within first 24 hours of life, abdominal distension, and vomiting. The severity of these symptoms and the degree of consitpation vary considerably between patients. HD is suspected on the basis of history and clinical findings and the diagnosis is established by radiological examination, anorectal manometry, and histochemical analysis of biopsy specimens. In this review, the advantages and pitfalls of each diagnostic modality are discussed. And a diagnostic approach utilizing these diagnostic modalities in children with suspicious HD is presented.