• Journal of Microbiology and Biotechnology
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• v.16 no.8
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• pp.1325-1329
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• 2006

Structural modeling of $\beta$-galactosidase from L. lactis ssp. lactis 7962 has shown that the residues Cys-472 and His-509 are located in the wall of the active-site cavity. To examine the functions of Cys-472 and His-509, we generated five site-specific mutants: Cys-472-Ser, Cys-472-Thr, Cys-472-Met, His-509-Asn, and His-509-Phe. $\beta$-Galactosidase substituted at Cys-472 with Met or His-509 with Phe had <3% of the activity of the native enzyme when assayed using ONPG as substrate. The other mutants Cys-472-Ser, Cys-472-Thr, and His-509-Asn had ca. 10-15% of the native enzyme activity. The V$_max$ values of the five mutated enzymes were lower (60-7,000-fold) than that of native enzyme. These results show that the catalytic ability of $\beta$-galactosidase is significantly affected by mutations at Cys-472 or His-509.

• 한국정보디스플레이학회:학술대회논문집
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• 2009.10a
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• pp.509-509
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• 2009

In this presentation, the speaker draws upon his years of research into volumetric display systems. Key content from several books is used as a basis to discuss current activity and to provide a basis for discussion on the next generation of volumetric technologies.

• Korean Journal of Ecology and Environment
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• v.42 no.4
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• pp.502-509
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• 2009

In this study a total of 27 stream sites, at 1 km intervals, were monitored for simple physicochemical water characteristics, land coverage patterns, and stream environment characteristics using the Habitat-riparian Indexing System (HIS), in the Yangsan Stream. The HIS has been tested in previous research, resulting in some identification of advantages in the application to the stream ecosystems data. Even though reliable stream environment characterization was possible using HIS, there was no information about the application of this tool to present continuity of environmental changes in stream systems. Also the necessity was raised to compare the results of HIS application with land coverage information in order to provide useful information in management strategy development. The monitoring results of this study showed that changes of environmental degradation were well represented by HIS. Especially, stream environment degradation due to construction was relatively well reflected in the HIS monitoring results, and the main causality of Yangsan Stream degradation was expansion of the urbanized area. In addition, there were significant relationships between the HIS scores and land coverage information. Therefore, it is necessary to prepare appropriate options in controlling or managing the expansion of the industrialized areas in this stream basin in order to improve the stream environment. For this purpose, ensemble utilization of HIS results, water quality, and geographical information, resulting in integration with remote sensing processes can be possible.

• Journal of Chest Surgery
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• v.24 no.5
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• pp.505-509
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• 1991

Rupture or laceration of the aorta is a more common result of nonpenetrating traumatic injury than is generally appreciated. If the lesion is promptly diagnosed, a appropriate surgical treatment may be life-saving. Diagnosis may be difficult and at times the rupture may remain clinically silent for variable period.< A 34 - year old male patient had sustained steering wheel injury to his chest during automobile accident 8 weeks prior to admission. The diagnosis of traumatic aneurysm of the aorta was delayed as he was asymptomatic. Surgical repair of false aneurysm of the descending aorta was successfully performed by partial cardiopulmonary bypass through the femoral artery and vein.

• Journal of English Language & Literature
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• v.54 no.4
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• pp.483-509
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• 2008

For the last decades, criticism on Frankenstein has tried to make a link between Victor's Creature and Rousseaurean "man in a state of nature." Like the Rousseaurean savage in a state of animal, the monster has only basic instincts least needed for his survival, i.e. self-preservation, but turns into a civilized man after learning language. Most critics argue that, despite the monster's acquisition of language, his failure in entry into a cultural and linguistic community is the outcome of a lack of sympathy for him by others, which displays the stark existence of epistemological barriers between them. That is to say, the monster imagines his being the same as others in the pre-linguistic stage but, in the linguistic stage, he realizes that he is different from others. Interpreting the Rousseaurean idea of language, which appears in his writings, as much more focused on emotion than many critics think, I read the dispute between Victor and his Creature as a variation of parent-offspring conflict. Shelley criticizes Rousseau's parental negligence in putting his children into a foundling hospital and leaving them dying there. The monster's revenge on uncaring Victor parallels the likely retaliation Rousseau's displaced children would perform against Rousseau, which Shelley imaginatively reproduces in her novel. The conflict between the monster and Victor is due to a disrupted attachment between parent and child in terms of Darwinian developmental psychology. Affective asynchrony between parent and child, which refers to a state of lack of mutual favorable feelings, accounts for numerous dysfunctional families. This paper shifts a focus from a semiotics-oriented perspective on the monster's social isolation to a Darwinian perspective, drawing attention to emotional problems transpiring in familial interactions. In doing so, it finds that language is a means of communicating one's internal emotions to others along with other means such as facial expressions and body movements. It also demonstrates that how to promote emotional well-being in either familial or social relationships entirely depends on the way in which one employs language that can entail either pleasure or anger on hearers' part.

• Journal of Life Science
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• v.9 no.5
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• pp.504-509
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• 1999

The trend of organogenesis in Xenopus presumptive ectoderm was studied by combined dose of activin A and IGF-1(insulin-like growth factor-1). In reference study of Asashima and his colleagues, the inductive patterns of various organs were reported with activin, the potent mesoderm inducing factor. In present study, the inducing pattern was cleared with combined-dose of concentration 1-100 ng/ml activin A and IGF-1. In addition, the result from single treatment of activin A was compared with former study. As a result, eye was differentiated in 5-20% of explants at 10 and 50 ng/ml concentrative combination of activin A. Otic vesicle was appeared in the entire concentrative combination of IGF-1. Pronephric duct was induced 19-38% of explants at the concentration of activin A 100 ng/ml by adding IGF-1. The comparison of single treatment of activin A was showed some difference in dose-dependent inducing pattern.

• (The)Study of the Eastern Classic
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• no.69
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• pp.509-530
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• 2017

The purpose f this thesis is to examine the reality of Min Sha-pyeong, which led to life in the end of the Koryo Dynasty. Min Sha-pyeong was able to conduct academic exchanges through active negotiations with various scholars at the time. After passing the civil service exam at the age of 21, Min Sha-pyeong acted in various positions and remained loyal to the government. Min Sha-pyeong, who frequently met with Lee Je-hyun, Baek Mun-bo and Lee-saek, exchanged opinions. Sometimes Baek Mun-bo gathered together and always joined him when drinking. Lee-saek was convinced that his poetry would continue to inspire. The time when Min Sha-pyeong was active very confusing at home and abroad. In the end of the Koryo Dynasty, the national operating system was unstable due to the poor land and the suffering of the afflicted people, who suffered from frequent in vasions of Japan. At the time, the corruption and the suffering of the people were serious. Min Sha-pyeong refers to the reality of the people who love the people, reflecting the lives of the people living in poverty. Min Sha-pyeong thinks life without loyality is not a normal life. If I had a desire, I realized that the riches of wealth would wane someday. Min Sha-pyeong hoped that he would be relieved of his sympathy and would be relieved of their position in sympathy ith the people. Here, youy can see the reality of the people in Min Sha-pyeong is clearly revealed.

• Journal of Physiology & Pathology in Korean Medicine
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• v.29 no.6
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• pp.503-509
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• 2015

This is a case report on a deep vein thrombosis(DVT) patient whose pain, numbness, and swelling in the left leg have been decreased with Korean Medicine. The 59 year-old male patient who was diagnosed with DVT and showed the classic symptoms of DVT(pain, numbness and swelling in the left leg). Although he was treated with medication(100mg of aspirin, 20mg of rivaroxaban) for five weeks, his symptoms did not improved. Then, he was gaven Sunkihwalhyul-tang three times a day and Jungsongouhyul pharmacopuncture once a day for two months. Conservative therapy including leg elevation and an elastic stocking was co-administered. During our treatment period, the size of the thrombus in the left external iliac vein was decreased according to sonography compared with the pre-hospital's sonography, and the patient felt his symptoms had improved. This case report suggests Korean Medicine may play a role in improving the deep vein thrombosis symptoms.

• The Korean Journal of Community Living Science
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• v.19 no.4
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• pp.509-518
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• 2008

The purpose of this study was to explore how job characteristics, housework factors (reciprocity) and family characteristics influence the health status of a husband and wife. To do this, the study used the ordered probit estimation model. The results of this study were as follows: the status of the husband's health was perceived to be slightly better than that of the wife. age, the husband's job satisfaction and wife's future job prospects were found to impact upon the health status of both the husband and the wife. In addition, the health of the husband was found to be significantly influenced by household income and his wife's perceived fairness of housework. On the other hand, the influencing factors upon the health status of the wife were her education, child caring responsibilities, her husband's future job prospects, as well as the flexibility of her work schedule and hours of work.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.27 no.11C
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• pp.1090-1096
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• 2002

The HTTP does not support continuity for browser-server interaction between successive visits or a user due to a stateless feature. Cookies were invented to maintain continuity and state on the Web. Because cookies are transmitted in plain and contain text-character strings encoding relevant information about the user, the attacker can easily copy and modify them for his undue profit. In this paper, we design a secure cookies scheme based on X.509 public key certificate for solving these security weakness of typical web cookies. Our secure cookies scheme provides not only mutual authentication between client and server but also confidentiality and integrity of user information. Additionally, we implement our secure cookies scheme and compare it to the performance with SSL(Secure Socket Layer) protocol that is widely used for security of HTTP environment.