• Restorative Dentistry and Endodontics
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• v.35 no.4
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• pp.285-294
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• 2010

The purpose of this study was to evaluate the viability of periodontal ligament cells of rat teeth after low-temperature preservation under high pressure by means of MTT assay, WST-1 assay. 12 teeth of Sprague-Dawley white female rats of 4 week-old were used for each group. Both side of the first and second maxillary molars were extracted as atraumatically as possible under tiletamine anesthesia. The experimental groups were group 1 (Immediate extraction), group 2 (Slow freezing under pressure of 3 MPa), group 3 (Slow freezing under pressure of 2 MPa), group 4 (Slow freezing under no additional pressure), group 5 (Rapid freezing in liquid nitrogen under pressure of 2 MPa), group 6 (Rapid freezing in liquid nitrogen under no additional pressure), group 7 (low-temperature preservation at $0^{\circ}C$ under pressure of 2 MPa), group 8 (low-temperature preservation at $0^{\circ}C$ under no additional pressure), group 9 (low-temperature preservation at $-5^{\circ}C$ under pressure of 90 MPa). F-medium and 10% DMSO were used as preservation medium and cryo-protectant. For cryo-preservation groups, thawing was performed in $37^{\circ}C$ water bath, then MTT assay, WST-1 assay were processed. One way ANOVA and Tukey HSD method were performed at the 95% level of confidence. The values of optical density obtained by MTT assay and WST-1 were divided by the values of eosin staining for tissue volume standardization. In both MTT and WST-1 assay, group 7 ($0^{\circ}C$/2 MPa) showed higher viability of periodontal ligament cells than other group (2-6, 8) and this was statistically significant (p < 0.05), but showed lower viability than group 1, immediate extraction group (no statistical significance). By the results of this study, low-temperature preservation at $0^{\circ}C$ under pressure of 2 MPa suggest the possibility for long term preservation of teeth.

• Journal of the Korea Organic Resources Recycling Association
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• v.25 no.2
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• pp.41-48
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• 2017

A vertical multi-stage ammonia stripping reactor using E-PFR, which has been proved to be superior in anaerobic and aerobic treatment, was developed and a lab scale experiment was conducted. According to the change of stage number condition, the removal rate of the ammonia nitrogen in the reactor with 0-stage was about 52.5% after 8 hours (pH 10, temperature $35^{\circ}C$, and the air/liquid ratio $3min^{-1}$) However, in the reactor with 5-stage, the removal efficiency was about 62.6%. According to the change of pH condition, the removal rate of ammonia nitrogen was about 42.6% at pH 9 after 8 hours, and was about 74.4% at pH 11 (5-stage reactor, temperature $35^{\circ}C$, and the air/liquid ratio $3min^{-1}$). According to the change of temperature condition, the removal rate of the ammonia nitrogen was about 51% at $25^{\circ}C$ after 8 hours (5-stage reactor, pH 10, and the air/liquid ratio $3min^{-1}$), and was about 87.2% at $45^{\circ}C$. According to the change of air injection volume condition, the removal rate of the ammonia nitrogen was about 45.8% at $2min^{-1}$ after 8 hours (5-stage reactor, pH 10, and at $35^{\circ}C$). and was about 75% at $4min^{-1}$. Based on these results, we will follow up the applicability of the actual plant in the future through continuous operation evaluation.

• Journal of Veterinary Clinics
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• v.27 no.4
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• pp.348-365
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• 2010

Artemisia capillaries THUNB is a perennial herb that belongs to the family compositae spp. and the most common plant among the various herbal folk remedies used in treatment of abdominal pain, hepatitis, chronic liver disease, jaundice and coughing in Korea. The object of this study is to observe the dosage-dependent anti-obestic effects of an aqueous extracts of dried aqueous extracts of stems of Artemisia capillaris Thunberg. [Artemisiae capillaris Herba, In-Jin in Korean, INJ] on 45%/Kcal high fat diet (HFD) supplied mice. 45%/Kcal rodent HFD are supplied to ICR mice from 1 week before initiation of INJ administration throughout the 12 weeks, and after the end of 12 weeks of 62.5, 125 and 250 mg/kg/day of INJ administration, the efficacy was divided into five categories 1) hypoglycemic, 2) hepato-protective, 3) nephroprotective, 4) hypolipemic, and 5) anti- obesity effects. The effects were compared to those of simvastatin (for hypolipemic activity), silymarin (for hepatoprotective and free radical scavenger effects) and metformin (for hypoglycemic and related anti-obesity effects). 7 animals per group (8 groups; total 56adapted mice on HFD were selected base on the body weight at 6 days after initiation of HFD supply) were used in this experiment. INJ and all three different reference drugs were directly suspended or dissolved in distilled water, and administered at a volume of 10 mL/kg, once a day for 84 days from 1 week after HFD supply. As results of 91 days of continuous HFD supply, mice showed marked obese states, hyperglycemia, hyperlipemia, liver damages and kidney damages. These mean the obesity, diabetes, diabetic hepatopathies, nephropathies and hyperlipemia were induced by HFD supply. After end of 84 days of continuous treatment of three different dosages of INJ, all diabetes related complications were inhibited; relatively favorable anti-obesity, hypolipemic, hepatoprotective, hypoglycemic and nephroprotective effects. These favorable effects showed relatively good dose-relations between all three different dosages of INJ treated, and INJ 250 mg/kg showed enough favorable effects on diabetes and related four complications tested as compared with one of each three different references. Otherwise, the efficacy of 62.5 and 125 mg/kg of INJ was somewhat slighter than those of all three reference drugs. Therefore, the suitable effective dosage of INJ is considered as 250 mg/kg/day in the present study. The overall anti-obesity effects of INJ 250 mg/kg-treated group was similar or more favorable than those of metformin 250 mg/kg-treated group, and INJ 250 mg/kg showed slighter hypoglycemic effects with silymarin 100 mg/kg and metformin 250 mg/kg, similar hypolipemic effects with simvastatin 10 mg/kg, and similar hepatoprotective effects with silymarin 100 mg/kg, and similar nephroprotective effects with that of silymarin 100 mg/kg and metformin 250 mg/kg, respectively. Obese, hyperglycemia, hyperlipemia, steatohepatitis and related nephropathies induced by HFD supply were dramatically inhibited by 84 days of continuous treatment of all three different dosages of INJ. It is, therefore expected that INJ extracts will be a favorable alternative agent for diet-related diabetes and complications.

• Korean Journal of Organic Agriculture
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• v.9 no.2
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• pp.101-115
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• 2001

Nitrogen applied as fertilizer for crop production is partly absorbed by plant , and the remaining nitrogen in soil might be leached out through complicated processes to the subsoil layer Especially, NO$_3$-N in leachate causes environmental pollution. The purpose of this study was focused on understanding of uptake of nutrients by plants, the behaviors of nutrients in soil and the possibility of leaching loss when nitrogen fertilizer and completely decomposed compost were applied. Lysimeters(Volume 0.15㎥, Diameter 62cm, Height 62.8cm) were installed for collecting leachate in the Jeju volcanic ash soils. Lysimeter study consisted of thirteen treatments : fallow, fallow with weeding, cropping without fertilizer and compost, three N fertilizer soil surface applications(16, 32, 64kg/10a), three N fertilizer and compost soil surface applications(16+800, 32+1600, 64+32kg/10a), two water dissolved N fertilizer applications(16, 32kg/10a), and low and high plant densities. N fertilizer was applied as urea. The growth of com(preceding crop) and potatoes(succeeding crop) and leaching loss were determined during the experimental period. The results obtained were summarized as follows ; With Increased N, pH of leachate tended to decrease and NO$_3$-N concentration of leachate increased. NO$_3$-N leaching loss was remarkably greater in soil from the bare plot without fertilization and the weed control than from plots with medium N rate and was least in the cropping plot without fertilization. NO$_3$-N concentration in leachates from the water dissolved N fertilizer application plots was 64% of that from the soil surface application plots. The concentration of Ca and K ions and the leaching loss of these ions were least from the cropping plot without fertilization and were greatest from bare plots(T1 and T2) without fertilization. The proportion of leaching and residual N in soil increased as N rate increased indicting that higher N rates increase the possibility of N leaching to subsoil layer The proportion of N leaching losses was lower at the low N rate and the high plant density. In future, fertilization prescription which can maximize fertilizer use efficiency and minimize the pollution of ground water will be needed for conserving the environments.

• Proceedings of the Korean Radioactive Waste Society Conference
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• 2005.06a
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• pp.110-120
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• 2005

For utilizing vitrification to treat low and intermediate level waste, industrial pilot plant was designed and constructed in October 1999 at Daejon, Korea through the joint research program among NETEC, MOBIS and SGN. More than 70 tests were performed on simulated IER, DAW etc. including key nuclide surrogate(Cs, Co); this plant has been shown to vitrify the target waste effectively and safely, however, some dust are generated from the HTF(High Temperature Filter) as a secondary waste. In case of long term operation, it is also concerned that pipe plugging can be occurred due to deposited dust in cooling pipe namely, connecting pipe between CCM(Cold Crucible Melter) and HTF. In this regard, we have developed the special complementary system of the off-gas treatment system to recycle the dust from HTF to CCM and to remove the interior dust of cooling pipe. Main concept of the dust recycling is to feed the dust to the CCM as a slurry state; this system is regarded as of an important position in the viewpoint of volume reduction, waste disposal cost and glass melt control in CCM. The role of DRS(Dust Recycling System) is to recycle the major glass components and key nuclides; this system is served to lower glass viscosity and increase waste solubility by recycling B, Na, Li components into glass melt and also to re-entrain and incorporate into glass melt like Cs, Co. Therefore dust recycling is helpful to control the molten glass; it is unnecessary to consider a separate dust treatment system like a cementation equipment. The effects of Dust Cleaner are to prevent the pipe plugging due to dust and to treat the deposited dust by raking the dust into CCM. During the pilot vitrification test, overall performance assessment was successfully performed; DRS and Dust Cleaner are found to be useful and effective for recycling the dust from HTF and also removing the dust in cooling pipe. The obtained operational data and operational experiences will be used as a basis of the commercial facility.

• Journal of Animal Science and Technology
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• v.50 no.4
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• pp.561-572
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• 2008

Responses of real-time control parameters, such as ORP, DO and pH, to the conditions of biological animal wastewater treatment process were examined to evaluate the stability of real-time control using each parameter. Also an optimum index for supplemental carbon source addition based on NOx-N level was determined under a consideration of denitrification rate by endogenous respiration of microorganism and residual organic matter in liquor. Experiment was performed with lab-scale sequencing batch reactor(SBR) and working volume of the process was 45L. The distinctive nitrogen break point(NBP) on ORP-and DO-time profiles, which mean the termination of nitrification, started disappearing with the maintenance of low NH4-N loading rate. Also the NBP on ORP-and DO-time profiles was no longer observed when high NOx-N was loaded into the reactor, and the sensitivity of ORP became dull with the increase of NOx-N level. However, the distinctive NBP was constantly occurred on pH(mV)-time profile, maintaining unique profile patterns. This stable occurrence of NBP on pH(mV)-time profile was lasted even at very high NOx-N:NH4-N ratio(over 80:1) in reactor, and the specific point could be easily detected by tracking moving slope change(MSC) of the curve. Revelation of NBP on pH(mV)-time profile and recognition of the realtime control point using MSC were stable at a condition of over 300mg/L NOx-N level in reactor. The occurrence of distinctive NBP was persistent on pH(mV)-time profile even at a level of 10,000mg/L STOC(soluble total organic carbon) and the recognition of NBP was feasible by tracing MSC, but that point on ORP and DO-time profiles began to disappear with the increase of STOC level in reactor. The denitrfication rate by endogenous respiration and residual organic matter was about 0.4mg/L.hr., and it was found that 0.83 would be accepted as an index for supplemental carbon source addition when 0.1 of safety factor was applied.

• Tuberculosis and Respiratory Diseases
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• v.48 no.3
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• pp.357-364
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• 2000

Background : Complicated exudative pleural fluid collections have traditionally been treated by either closed tube thoracostomy drainage or by open surgical drainage. Complete drainage is important in order to control pleural sepsis, restore pulmonary function, and entrapment. Recently intracavitary fibrinolytic therapy has been advocated as a method to facillitate drainage of complicated exudative pleural effusion and to allow enzymatic debridemant of the restrictive fibrinous sheets covering the pleural surface. The purpose of this study is to prospectively evaluate the effects of image-guided catheter drainage with high dose urokinase(UK) instillation in the treatment of complicated pleural effusions. Patients : Twenty complicated pleural effusion patients that poorly respond to image-guided drainage were allocated to receive UK. There were 8 pneumonia and 12 tuberculosis. Methods : Drugs were diluted in 250 mL normal saline and were infused intrapleurally through the chest tube or pig-tail catheter in a daily dose of 250,000 IU of UK. Response was assessed by clinical outcome, fluid drainage, chest radiography, pleural ultrasound and/or computed tomography. Results : The mean UK instillation time was $1.63{\pm}0.10$. The mean volume drained UK instillation was $381.3{\pm}314.4\;mL$, and post-UK was $321.6{\pm}489.5\;mL$. The follow up duration after UK therapy was mean $212.9{\pm}194.5$ days. We had successful results in 19 cases (95.0%). There were 12 pleural thickenings (60.0%), 2 markedly decreased effusions (10.0%) and 5 cases of no thickening or effusion. There was recurrence after treatment in only one patient(5%) with complicated pleural effusiondue to tuberculosis. Conclusions : Image-guided drainage with high dose UK instillation (250,000 U/day) in complicated pleural effusion is a safe and more effective method than closed thoracostomy drainage. And this management, in turn, can obviate surgery in most cases.

• Tuberculosis and Respiratory Diseases
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• v.50 no.4
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• pp.415-425
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• 2001

Background : The correlation between the high resolution computed tomography(HRCT) emphysema score and the physiologic parameters including resting and exercise pulmonary function test was investigated in 14 patients($60.6{\pm}10.3$ years) with pulmonary emphysema. Methods : The patients underwent a HRCT, a resting pulmonary function test, and incremental exercise testing(cycle ergometer, 10 W/min). Computed tomography scans were obtained on a GE highlight at 10 mm intervals using 10 mm collimation, from the apex to the base after a full inspiration. The emphysema scores were determined by a CT program 'Density mask' outlining the areas with attenuation values less than -900 HU, indicating the emphysema areas, and providing an overall percentage of lung involvement by emphysema. Results : Among the resting PFT parameters, only the diffusing capacity(r=-0.75) and $PaO_2$ (r=-0.66) correlated with the emphysema score(p<0.05). Among the exercise test parameters, the emphysema score correlated significantly with the maximum power(r=-0.74), maximum oxygen consumption(r=-0.68), anaerobic threshold(V-slope method: r=-0.69), maximal $O_2$-pulse(r=-0.73), and the physiologic dead space ratio at the maximum workload(r=-0.80)(p<0.01). Conclusion: We could find that exercise testing parameters showed a much better correlation with the HRCT emphysema score, which is known to have a good correlation with the pathologic severity than the resting PIT parameters. Therefore it is suggested that exercise testing is superior to resting PIT for estimating in the estimation of the physiologic disturbance in emphysema patients.

• Korean Journal of Agricultural and Forest Meteorology
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• v.1 no.1
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• pp.41-51
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• 1999

This study was conducted to investigate the effects of local climatic conditions on the annual increment of Korean white pine planted in Gapyung and Yaungdong. For this, stand variables such as mean DBH, mean height, basal area per hectare, and volume per hectare by stand age were measured and summarized for each locality. Based on these statistics, annual increments for 8 years from stand age 10 to 18 were calculated for each of stand variables. A topoclimatological technique which makes use of empirical relationships between the topography and the weather in study sites was applied to produce normal estimates of monthly mean, maximum, minimum temperatures, relative humidity, precipitation, and hours of sunshine. Then, the yearly climatic variables from 1990 to 1997 for each study site were derived from the spatial interpolation procedures based on inverse- distance weighting of the observed deviation from the climatic normals at the nearest 11 standard weather stations. From these estimates, 17 weather variables such as warmth index, coldness index, index of aridity etc., which affect the tree growth, were computed on yearly base for each locality. The deviations of measured annual increments from the expected annual increments for 8 years based on yield table of Korean white pine were then correlated with and regressed on the yearly weather variables to examine effects of local climatic conditions on the growth. Gapyung area provides better conditions for the growth of Korean white pine in the early stage than Youngdong area. This indicates that the conditions such as low temperature, high relative humidity, and large amount of precipitation provide favor environment for the early growth of Korean white pine. A ccording to the correlation and regression an analysis using local climatic conditions and annual increments, the growth pattern of Gapyung area corresponds to this tendency. However, it was found that the relationship between annual increments and local climatic conditions in Youngdong area shows different tendency from Gapyung. These results mean that the yearly growth pattern could not sufficiently be explained by climatic conditions with high variance in yearly weather variables. In addition, the poor growth in Youngdong area might not only be affected by climatic conditions, but also by other environmental factors such as site quality.

• Journal of the Korean Society of Food Science and Nutrition
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• v.35 no.10
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• pp.1385-1393
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• 2006

The purpose of this study was to investigate nutrient intake status and dietary behaviors in elementary school children. The subjects were 98 boys and girls in the 4~6 grade and divided into three groups by body shape. The protein intakes of the subjects were higher than the Korean EER (estimated energy requirements). Especially male over weight group had more than twice intakes (216.0% ) comparing the Korean EER. The mean daily intakes of calcium, iron, vitamin A and vitamin C in all subjects did not meet the Korean RI (recommended intake). The vitamin C intake of male underweight group was only 43.2% comparing the Korean RI. Rice & cereals were main food sources of energy, protein and iron, and milk & milk products were main food sources of calcium. As their weights were high, the meal volume and the rate of skipped meal were shown to be high (p<0.05). Thus, this study implicates that desirable nutrition education may be needed for overweight and underweigh children.