• Korean Journal of Clinical Laboratory Science
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• v.51 no.1
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• pp.78-85
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• 2019

Membrane proteins are involved in many common diseases, including heart disease and cancer. In various disease states, such as cancer, abnormal signaling pathways that are related to the membrane proteins cause the cells to divide out of control and the expression of membrane proteins can be altered. Membrane proteins have the hydrophobic environment of a lipid bilayer, which makes an analysis of the membrane proteins notoriously difficult. Therefore, this study evaluated the efficacy of two different methods for optimal membrane protein extraction. High-speed centrifuge and reagent-based method with a -/+ filter aided sample preparation (FASP) were compared. As a result, the high-speed centrifuge method is quite effective in analyzing the mitochondrial inner membranes, while the reagent-based method is useful for endoplasmic reticulum membrane analysis. In addition, the function of the membrane proteins extracted from the two methods were analyzed using GeneGo software. GO processes showed that the endoplasmic reticulum-related responses had higher significance in the reagent-based method. An analysis of the process networks showed that one cluster in the high-speed centrifuge method and four clusters in the reagent-based method were visualized. In conclusion, the two methods are useful for the analysis of different subcellular membrane proteins, and are expected to assist in selecting the membrane protein extraction method by considering the target subcellular membrane proteins for study.

• Journal of the Korean Society of Manufacturing Technology Engineers
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• v.19 no.4
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• pp.576-581
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• 2010

As a super high speed small-sized centrifugal separator which is operated by the center inputting method has solved two difficulty problems, it can be made use of developing the new composition through the making of Nano particle (50~100nm) by high speed revolution and alleviation of unbalanced shapes inside the disk continuously throwing slurry in the central part. Uses the air spindle and the centrifugal machine production will be able to manufacture the Roh class fine particle under boil.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 1997.04a
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• pp.312-317
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• 1997

This paper present an optimum design for the rotor-bearing system of a high-speed (80000RPM) ultra-centrifuge supported by ball bearings with nonlinear stiffness characteristics. To obtain the nonlinear bearing stiffnesses, a ball bearing is modelled in five degrees of freedom and is analyzed quasi-statically. The dynamic behaviors of the nonlinear rotor-bearing system are analyed by using a transfer-matrix method iteratively. For optimum design, minimizing the weight of a rotor is used as a cost function and the Augmented Lagrange Multiplier (ALM) method is employed. The result shows that the rotor-bearing system is optimized to obtain 8% weight reduction.

• Journal of the Korean Geotechnical Society
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• v.32 no.4
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• pp.5-14
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• 2016

Numerical simulation of dynamic soil-pile-structure interaction embedded in a dry sand was carried out. 3D model of the dynamic centrifuge model tests was formulated in a time domain to consider nonlinear behavior of soil using the finite difference method program, FLAC3D. As a modeling methodology, Mohr-Coulomb criteria was adopted as soil constitutive model. Soil nonlinearity was considered by adopting the hysteretic damping model, and an interface model which can simulate separation and slip between soil and pile was adopted. Simplified continuum modeling (Kim et al., 2012) was used as boundary condition to reduce analysis time. Calibration process for numerical modeling results and test results was performed through the parametric study. Verification process was then performed by comparing numerical modeling results with another test results. Based on the calibration and validation procedure, it is identified that proposed modeling method can properly simulate dynamic behavior of soil-pile system in dry condition.