• Journal of Animal Science and Technology
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• v.63 no.1
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• pp.16-24
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• 2021

The suppressive effect of monensin as an ionophore-feed additive on enteric methane (CH4) emission and renewable methanogenesis were evaluated. To clarify the suppressive effect of monensin a respiratory trial with head cage was performed using Holstein-Friesian steers. Steers were offered high concentrate diets (80% concentrate and 20% hay) ad libitum with or without monensin, galacto-oligosaccharides (GOS) or L-cysteine. Steers that received monensin containing diet had significantly (p < 0.01) lower enteric CH4 emissions as well as those that received GOS containing diet (p < 0.05) compared to steers fed control diets. Thermophilic digesters at 55℃ that received manure from steers fed on monensin diets had a delay in the initial CH4 production. Monensin is a strong inhibitor of enteric methanogenesis, but has a negative impact on biogas energy production at short retention times. Effects of the activity of coprophagous insects on CH4 and nitrous oxide (N2O) emissions from cattle dung pats were assessed in anaerobic in vitro continuous gas quantification system modified to aerobic quantification device. The CH4 emission from dungs with adults of Caccobius jessoensis Harold (dung beetle) and the larvae of the fly Neomyia cornicina (Fabricius) were compared with that from control dung without insect. The cumulative CH4 emission rate from dung with dung insects decreased at 42.2% in dung beetles and 77.8% in fly larvae compared to that from control dung without insects. However, the cumulative N2O emission rate increased 23.4% in dung beetles even though it reduced 88.6% in fly larvae compared to dung without coprophagous insects. It was suggested that the antibacterial efficacy of ionophores supplemented as a growth promoter still continued even in the digested slurry, consequently, possible environmental contamination with the antibiotics might be active to put the negative impact to land ecosystem involved in greenhouse gas mitigation when the digested slurry was applied to the fields as liquid manure.

• Fisheries and Aquatic Sciences
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• v.23 no.1
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• pp.1.1-1.14
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• 2020

Background: Dietary protein requirements are dependent on a variety of factors and water temperature is one of the most important abiotic factors affecting protein requirement of fish. This study was, therefore, conducted to investigate effects of water temperature on dietary protein requirement of fry Heteropneustes fossilis which has high demand in most of the Asian markets. Methods: Quadruplicate groups of 30 fish per treatment (2.97 ± 0.65 cm; 5.11 ± 0.34 g) were fed seven isoenergetic diets (17.9 kJ g^-1 gross energy; 14.99 kJ g^-1 digestible energy) containing dietary protein levels ranging from 28 to 52% at two water temperatures (18 and 26 ℃). Experimental diets were fed to apparent satiation as semi-moist cakes thrice daily at 17:00, 12:00, and 17:30 h for 12 weeks. For precise information, various growth parameters, protein deposition, hematological parameters, metabolic enzymes, and stress response were analyzed, and effects of water temperature on dietary protein requirement was recommended on the basis of response from above parameters. Results: Groups held at 26 ℃ attained best growth, feed conversion, and protein deposition at 44% dietary protein indicating that temperature affected dietary protein requirement for optimum growth of H. fossilis fry and protein requirement seems to be satisfied with 44% dietary protein. Interestingly, interactive effects of both dietary protein levels and temperature were not found (P > 0.05). Fish reared at 18 ℃ had comparatively higher values for aspartate and alanine transferases than those reared at 26 ℃ water temperature which exhibited normal physiological value for these enzymes indicating that body metabolism was normal at this temperature. Hematological parameters also followed same pattern. Furthermore, fish reared at 26 ℃ water temperature exhibited more resistant to thermal stress (P < 0.05). The 95% maximum plateau of protein deposition data using second-degree polynomial regression analyses exhibited dietary protein requirement of fry H. fossilis between 40.8 and 41.8% of diet at 26 ℃ water temperature. The recommended range of dietary protein level and protein/digestible energy ratio for fry H. fossilis is 40.8-41.8% and 27.21-27.88 mg protein kJ^-1 digestible energy, respectively. Conclusions: Information developed is of high significance for optimizing growth potential by making better utilization of nutrient at 26 ℃ and, to develop effective management strategies for mass culture of this highly preferred fish species.

• Journal of Nutrition and Health
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• v.35 no.9
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• pp.903-911
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• 2002

The effects of dietary levan, high-molecular-weight $\beta$-2,6-linked fructose polymer, on adiposity, serum leptin and UCP expression in rats fed high fat diet were studied. The adipose tissue hormone, leptin has been proposed to be involved in the regulation of food intake and energy expenditure. Uncoupling protein (UCP), a mitochondrial protein that uncouples the respiratory chain from oxidative phosphorylation, generates heat instead of ATP, thereby increase energy expenditure. To determine whether the dietary levan may have the anti-obesity effect, 4 wk old Sprague Dawley male rats fed high fat diet for 6 wks to induce obesity, and subsequently fed one of three diets for further 6 wks: 1) high fat (40% of calories) diet without levan 2) with 3% (w/w) levan 3) with 5% levan. For the comparison, control group fed AIN-76A diet. Visceral and peritoneal fat masses were lower in high fat diet with levan groups compared to high fat diet group. The effect of levan was dose-dependent. Adipocyte size was significantly reduced in the levan diet groups compared to the no levan diet group. Serum cholesterol level was not affected by levan containing diet, while the serum HDL cholesterol level was higher in leven diet groups. In addition, serum triglyceride level was markedly reduced by levan containing diet, thus lower than that of control group. Serum leptin was reduced by levan containing diet and lower in 5% levan group compared to 3% levan group (p < 0.001), as a result, serum leptin and insulin levels of 5% levan group were reduced to level of control group. Futhermore, the serum leptin level reflected the adiposity. The expression of UCP 1, and UCP 2 in brown adipose tissue was up-regulated by levan containing diet. In conclusions, levan containing diet reduced adiposity and serum triglyceride but increased UCP expression in the obese rats fed high fat diet. (Korean J Nutrition 35(9) : 903~911, 2002)

• Korean Journal of Poultry Science
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• v.23 no.1
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• pp.27-37
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• 1996

This experiment was carried out to study the effects of feeding split diets for a.m. and p.m. on laying hen performance and feed cost with 480 ISA Brown layers for three periods during 32∼36, 52∼56 and 72∼76 wk of age, respectively. The control group(C) was fed a conventional single diet throughout the day and split diet groups (T$_1$, T$_2$, T$_3$, T$_4$ and T$\_$5/) were offered high energy-high protein-low Ca diets, and low energy-low protein-high Ca diets in a.m. and p.m., respectively. In split diet groups, feed intake, ME and CP consumption, and feed cost were significantly reduced(P<0.05) compared to the C, while the hen-day egg production was significantly improved. However, the average egg weight was not influenced by the feeding system. Due to the reduced daily feed, ME, and CP intakes, and the improved egg production, the conversions of feed, ME, CP, and feed cost required per kg egg mass were also significantly improved (P<0.05) in splits diet groups. Among split diet groups, the T$_2$ group fed the diet devoid of Ca supplement from the conventional laying hen diet in a.m., and the diet containing the Ca supplement 2 times the conventional diet in p.m., was superior in conversion of nutrients and feed cost per kg egg mass to the other split diet groups. It was concluded that daily feed and nutrients cousumption and daily feed cost could be reduced 5∼6％ while hen-day egg production could be improved about 4％, so that about 10％ of the feed and nutrients and feed cost per kg egg mass could be spared by introducing the split diet feeding for a.m. and p.m. based on T$_2$ treatment in laying hens.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.11
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• pp.1593-1598
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• 2003

Nutritional evaluation of some agro-industrial byproducts available in Samoa [dry brewers' grains (DBG), cocoa shell (CS), cocoa dust (CD) and desiccated coconut waste meal (DCWM)] available in Samoa was carried out using both the in vivo and in vitro techniques. In the in vivo study 24 Anglo-nubian goats were offered by-products with other feed ingredients to compound four different diets. The goats were randomly allocated to 4 diets on the basis of liveweight (18.7-0.3kg). The ADF content of the byproducts followed a similar trend to NDF. The byproducts have a high content of organic matter (91.0-95.4%). Gross energy (GE) content was higher in DCWM (25.1 MJ/kg DM), closely followed by CD (23.2 MJ/kg DM). Concentrate intake was significantly different (p<0.05) among the goats. Average daily live weight gains were 105, 92, 88 and 97 g/goat/day for DBG, CS, CD and DCWM, respectively. Daily live weight gains were higher (p<0.05) in the goats that received DBG, while the least gain was obtained in the goats that received CS byproduct diet. DM digestibility was significantly higher (p<0.05) in the goats on DBG diet than in the other goats. The least DM digestibility was obtained in the goats that received CD diet (p>0.05). CP digestibility followed a similar pattern to DM digestibility. The digestibility of NDF and ADF was influenced by the nature of the diets. The digestibility of OM and GE were best (p<0.05) in the goats that received DBG, DCWM and CS byproduct diets than in CD. Significant differences (p<0.05) among the byproducts were recorded for net gas production. Potential gas production (a+b) ranged from 7.064 to 42.17 ml. Organic matter digested (OMD) from gas production value at 24 h was higher in DBG (47.6 g/kg DM) and this was followed by DCWM (42.5 g/kg DM). The least OMD was obtained in CD (17.9 g/kg DM). A significant difference (p<0.05) in DM disappearance after 4, 8, 16, 24, 48 and 72 h was recorded. The potential and effective degradability varied significantly (p<0.05) from 85.95-99.6 g/kg DM and from 39.9-65.8%, respectively. The digestibility of the byproducts in both the in vivo and in in vitro techniques demonstrated that they are potential source of feed ingredients for ruminant livestock in Samoa and possibly in the other small Pacific Island countries. On the basis of their potential degradability the byproducts could be ranked in the following order:DCWM>DBG>CD>CS. In conclusion, the results obtained suggest that all the byproducts can contribute to ruminant livestock diets without adverse effects on feed intake, growth rate and apparent nutrient digestibility coefficients.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.4
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• pp.502-511
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• 2005

The effects of different levels of crude protein (CP) and cottonseed meal replacing for soybean meal in cassava chips and rice straw-based diets for mid-lactating cows (100-150 day in milk [DIM]), were studied using 32 multiparous Holstein Fresian crossbred dairy cows. Diets containing 10.5, 12.5, 13.7, 14.4% CP of the rations and 0, 12.1, 14.9, 17.8% cottonseed meal were fed to cows for 60 days. Thirty two cows were randomly divided into four dietary treatments using a Randomized complete block design. Four dietary treatments were offered in the form of total mixed ration (TMR) with concentrate to roughage (chopped rice straw) at 60:40 and offered ad libitum. Dry matter (DM) and neutral detergent fiber (NDF) intakes tended to linearly increase with increasing dietary CP levels. Intakes and digestibility of crude protein increased linearly with increasing dietary CP level (p<0.01). Crude protein digestibility of the 10.5% CP diet was lower (p<0.05) than that in diets with higher levels of CP, while there were no significant differences among the other three levels of CP (12.5, 13.7 and 14.4%). Daily milk yield tended to increase with increased CP from 10.5 to 14.4%. Income over feed in terms of US$/kg of milk increased with increased CP from 10.5 to 13.7% and decreased when the CP level was higher than 13.7% (quadratic effect p<0.09). Milk composition was not significantly affected by increasing level of CP, however there were relatively high contents of protein and fat among treatments. The proportion of milk-urea N (MUN), ammonia-N ($NH_3$-N) and bloodurea N (BUN) were closely correlated and increased linearly with increasing CP levels (p<0.01). Balanced diet was found in diet containing 12.5 and 13.7% CP of the rations when BUN and MUN were used as indicators of the protein to energy ratio in the diet. Conclusions can be made that increasing dietary CP levels from 10.5 to 13.7% using cottonseed meal as the main source to completely replace soybean meal was beneficial to cows consuming rice straw and cassava chips based-diets. Increasing the CP level above 13.7% of total ration did not additionally improve milk yield and composition or net income.

• Journal of Nutrition and Health
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• v.33 no.4
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• pp.365-373
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• 2000

The purpose of this study was to compare the nutritional effects of resistant starch, cellulose and pectin on growth rate, metabolic energy and apparent digestibility in rats. Forty eight male Sprague-Dawley rats were randomly assigned to six dietary treatments, Rats were fed, ad libitum one of six experimental diets: 5% of resistant starch (RS), 5% of cellulose (CE), 5% of pection(PT), 2.5% resistant starch and 2.5% cellulose(RS+CE), 2.5% resistant starch and 2.5% pectin(RS+PT), and 2.5% cellulose and 2.5% pectin (CE+PT) during 4 weeks. The results obtained were as follows: body weight gain were higher in groups RS and CE, compared with that in the group PT, where other groups such as RS+CE, RS+PT and CE+PT showed no significant differences to the RS group. Food intake and food efficiency into showed the same patterns. Metabolic energy were similar in the RS and CE, and was the lowest the metabolic energy in the group CE+PT was significantly low. Apparent digestibility of protein was significantly higher in the group RS, compared with the groups CE and PT. Apparent digestibility of mixed dietary fiber groups such as the RS+CE, RS+PT and CE+PT group were also higher compared with the group PT. Apparent digestibility of lipid were significantly higher in the groups RS, CE and PT. Apparent digestibility of lipid in the mixed pectin groups such as the RS+PT and CE+PT growth rate, metabolic energy and apparent digestibility of lipid in the mixed pectin groups such as the RS+PT and CE+PT recorded the lowest, while that of the group RS+CE was significantly high. From above results, following was concluded: The growth rate, metabolic energy and apparent digestibility of protein and lipid of group RS were significantly higher in comparision with the group PT, while availability of energy nutrients of group CE was similar to that of the group RS. Also resistant starch fed with pectin(RS+PT) seemed to have increased the growth rate and the apparent digestibility of protein compared with those of the group PT fed with pectin(PT) alone, however RS+PT scemed to have the apparent digestibility of lipid.

• Journal of Nutrition and Health
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• v.56 no.1
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• pp.1-11
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• 2023

Purpose: Obesity is associated with alterations in vitamin D metabolism and elevation of parathyroid hormone (PTH). Increased PTH level in obesity is likely one of the factors contributing to the dysregulation of vitamin D metabolism. We investigated the effects of lowering the PTH level in high-fat diet-induced obese mice on vitamin D metabolism. Methods: Five-week-old male C57BL/6N mice were fed either with control (10% energy as fat) or high-fat (60% energy as fat) diets ad libitum for 12 weeks, and vehicle or cinacalcet HCl (30 ㎍/g body weight) was gavaged daily during the final week of the experiment. The following groups were studied: CON (control diet + vehicle), HFD (high-fat diet + vehicle), and HFD-CIN (high-fat diet + cinacalcet HCl). PTH, 1,25-dihydroxyvitamin D (1,25[OH]₂D), 25-hydroxyvitamin D (25[OH]D), calcium, and phosphate levels in circulation, and the expression of genes related to vitamin D metabolism in the liver and kidneys were determined. Results: Renal 1α-hydroxylase expression in the HFD group was higher than that in the CON group despite the lack of a difference in the PTH levels between the 2 groups. The plasma PTH level in the HFD-CIN group was 60% lower than that in the HFD group (p < 0.05). In parallel, the HFD-CIN group had lower adipose tissue amount (9% lower), renal 1α-hydroxylase expression (48% lower), and plasma 1,25(OH)₂D concentration (38% lower) than the HFD group. Conclusion: Lowering the PTH levels in high-fat diet-induced obese mice recovered the expression of renal 1α-hydroxylase and might be associated with lower amounts of white adipose tissue.

• Nutrition Research and Practice
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• v.3 no.1
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• pp.31-37
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• 2009

Recently, Korean people are consuming seaweeds almost 3.5 times more now than three decades ago. It is well known that seaweeds contain lots of soluble dietary fiber in addition to micronutrients such as ${\beta}$-carotene, iodine and some bioactive components. Seaweeds are considered to be effective for preventing chronic diseases including obesity, diabetes mellitus, atherosclerosis, cancer or constipation. This study was conducted to investigate the effect of seamustard intake on body weight gain, blood glucose level and lipid profiles in rats fed diets with different energy nutrient composition. Male Sprague-Dawley rats (average initial weight 103.7g) were divided into groups for two experiments as follows; Control, M2.5 & M5 groups (Exp. I) and M5, M10, HCM5, HCM10, HFM5 & HFM10 groups (Exp. II). The rats were fed diet and water ad libitum for 4 weeks. In general, there was no significant difference in blood glucose and triglyceride concentration among groups. In Exp. I, serum LDL-cholesterol level of rats fed diet with 5% seamustard powder (M5) was significantly lower than that of control group, while HDL-cholesterol level, TC/LDL ratio and weight of adrenal gland were higher. In Exp. II, food intake, body weight gain and EER of high fat diet with 10% seamustard group (HFM10) were the lowest among groups. Except gastrocnemius muscle, all organ weights of HFM10 group were the lowest. Fecal cholesterol excretion and serum LDL-cholesterol concentration of HFM10 group were the highest, while serum HDL-cholesterol level was the lowest among groups. Interestingly, HDL-cholesterol concentration was the highest in HCM5 group among groups. From these results, it was suggested that seamustard intake might be more effective for body weight control, but not for improving blood lipid profiles in high fat diet than in high carbohydrate diet.

• Journal of the Korean Society of Food Science and Nutrition
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• v.26 no.1
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• pp.116-122
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• 1997

Rats were adapted to diets containing 10% cellulose,10% sodium alginate and fiber-free diet for 5 weeks. Following a 14 hour fasting, rats were fed 5g of a test meal that provided 50% energy from fat, then killed at 4 hour postprandially. Plasma and lipoprotein fraction-cholesterol levels were lower in sodium alginate-fed animals than in rats fed other diets. Plasma TG did not differ among diet treatments. Increase in TG content of HDL fraction occurred in dietary fiber groups. Intestinal apolipoprotein B level and lipase activity were lower in sodium alginate-fed group than in other dietary groups. These results suggest that chronic consumption of sodium alginate affects plasma cholesterol level as in the case of fiber supplemetation, but is less likely to modify the acute Plasma TG response to high fat meal than if a fiber supplement is incorporated into the meal.