• Mass Spectrometry Letters
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• 제3권3호
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• pp.63-67
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• 2012

The need of heavy and unconventional crude oil as an energy source is increasing day by day, so does the importance of petroleomics: the pursuit of detailed knowledge of heavy crude oil. Crude oil needs techniques with ultra-high resolving capabilities to resolve its complex characteristics. Therefore, ultra-high resolution mass spectrometry represented by Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) has been successfully applied to the study of heavy and unconventional crude oils. The analysis of crude oil with high resolution mass spectrometry (FT-ICR MS) has pushed analysis to the limits of instrumental and methodological capabilities. Each high-resolution mass spectrum of crude oil may routinely contain over 50,000 peaks. To visualize and effectively study the large amount of data sets is not trivial. Therefore, data processing and visualization methods such as Kendrick mass defect and van Krevelen analyses and statistical analyses have played an important role. In this regard, it will not be an overstatement to say that the success of FT-ICR MS to the study of crude oil has been critically dependent on data processing methods. Therefore, this review offers introduction to peotroleomic data interpretation methods.

• BMB Reports
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• 제55권11호
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• pp.553-558
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• 2022

Hepatocellular carcinoma (HCC) is dangerous cancer that often evades early detection because it is asymptomatic and an effective detection method is lacking. For people with chronic liver inflammation who are at high risk of developing HCC, a sensitive detection method for HCC is needed. In a meta-analysis of The Cancer Genome Atlas pan-cancer methylation database, we identified a CpG island in the USP44 promoter that is methylated specifically in HCC. We developed methylation-sensitive high-resolution melting (MS-HRM) analysis to measure the methylation levels of the USP promoter in cell-free DNA isolated from patients. Our MS-HRM assay correctly identified 40% of patients with early-stage HCC, whereas the α-fetoprotein test, which is currently used to detect HCC, correctly identified only 25% of early-stage HCC patients. These results demonstrate that USP44 MS-HRM analysis is suitable for HCC surveillance.

• Mass Spectrometry Letters
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• 제2권3호
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• pp.65-68
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• 2011

The effects of column length and particle size on the efficiency of separation and characterization of phospholipids (PLs) are investigated using nanoflow liquid chromatography-electrospray ionization-tandem mass spectrometry (nLC-ESI-MS-MS). Since PLs are associated with cell proliferation, apoptosis, and signal transduction, it is of increasing interests in lipidomics to establish reliable analytical methods for the qualitative and quantitative profiling of PLs related to biomarker development in adult diseases. Due to the complexity of PLs, the preliminary separation of PLs is necessary prior to MS analysis. In this study, length of capillary column and the particle size of reversed phase ($C_{18}$) packing materials are varied to find a reliable condition for the high speed and high resolution separation using 8 PL standard mixtures. From experiments, it was found that a capillary column of nLC-ESI-MS-MS analysis for PL mixtures can be minimized to a 5 cm long pulled tip column packed with 3 ${\mu}m$ $C_{18}$ particles without losing resolution.

• 대한전자공학회논문지SD
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• 제43권12호
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• pp.55-64
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• 2006

본 설계에서는 무선 랜 등 최첨단 무선 통신 및 고급영상 처리 시스템과 같이 고해상도와 높은 신호처리속도, 저전력 및 소면적을 동시에 요구하는 고성능 집적시스템 응용을 위해 기존의 보정기법을 사용하지 않는 14b 70MS/s 0.13um CMOS A/D 변환기(Analog-to-Digital Converts- ADC)를 제안한다. 제안하는 がU는 중요한 커패시터 열에 인접신호에 덜 민감한 3차원 완전 대칭 구조의 레이아웃 기법으로 소자 부정합에 의한 영향을 최소화하였고, 3단 파이프라인 구조로 고해상도와 높은 신호처리속도와 함께 전력 소모 및 면적을 최적화하였다. 입력 단 SHA 회로에는 Nyquist 입력에서도 14비트 이상의 정확도로 신호를 샘플링하기 위해 게이트-부트스트래핑 (gate-bootstrapping) 회로를 적용함과 동시에 트랜스컨덕턴스 비율을 적절히 조정한 2단 증폭기를 사용하여 14비트에 필요한 높은 DC전압 이득을 얻음과 동시에 충분한 위상 여유를 갖도록 하였으며, 최종 단 6b flash ADC에는 6비트 정확도 구현을 위해 2단 오픈-루프 오프셋 샘플링 기법을 적용하였으며, 기준 전류 및 전압 발생기는 온-칩으로 집적하여 잡음을 최소화하면서 필요시 선택적으로 다른 크기의 기준 전압 값을 외부에서 인가할 수 있도록 하였다. 제안하는 시제품 ADC는 0.13um CMOS 공정으로 요구되는 2.5V 전원 전압 인가를 위해 최소 채널길이는 0.35um를 사용하여 제작되었으며, 측정된 DNL 및 INL은 14비트 해상도에서 각각 0.65LSB, 1.80LSB의 수준을 보이며, 70MS/s의 샘플링 속도에서 최대 SNDR 및 SFDR은 각각 66dB, 81dB를 보여준다. 시제품 ADC의 칩 면적은 $3.3mm^2$이며 전력 소모는 2.5V 전원 전압에서 235mW이다.

• 한국측량학회지
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• 제30권1호
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• pp.75-86
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• 2012

SAR는 기상상태와 태양고도 제약을 받지 않고 영상을 취득할 수 있는 장점을 갖지만 광학영상에 비해 시각적 가독성이 떨어지는 단점을 갖는다. 광학영상의 다중분광정보를 융합하여 SAR 영상의 가독성을 향상시키기 위한 다중센서 융합기술에 대한 관심이 증대되고 있다. 본 연구에서는 고속 퓨리에 변환을 통한 고주파 정보 추출 및 이상치 제거과정을 통해 SAR 영상의 공간적 세밀함과 다중분광영상의 분광정보를 유지할 수 있는 새로운 다중센서 융합기술을 제안하였다. 실험데이터로는 KOMPSAT-5호와 동일한 고해상도 X-band SAR 시스템을 장착한 TerraSAR-X 영상과 KOMPSAT-2호의 다중분광영상을 사용하였다. 제안기법의 효용성을 평가하기 위해 기존에 위성영상융합에 많이 사용된 융합기법과의 시각적/정량적 비교평가를 수행하였다. 실험 결과 기존 영상융합알고리즘에 비해 분광정보 보존측면에서 보다 향상된 결과를 보임을 확인할 수 있었다.

• Asian Pacific Journal of Cancer Prevention
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• 제15권2호
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• pp.891-894
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• 2014

This study is aimed to investigate the role of paired boxed gene 1 (PAX1) methylation analysis by methylation-sensitive high-resolution melting (MS-HRM) in the detection of high grade lesions in atypical squamous cells cannot exclude high-grade squamous intraepithelial lesion (ASC-H) and compared its performance with the Hybrid Capture 2 (HC2) human papillomavirus (HPV) test. In our study, 130 cases with a diagnosis of ASC-H from the cervical cytological screening by Thinprep cytologic test (TCT) technique were selected for triage. Their cervical scrapings were collected and evaluated by using PAX1 methylation analysis (MS-HRM) and high-risk HPV DNA test (HC2), followed by colposcopy and cervical biopsy. Chi-square test were used to test the differences of PAX1 methylation or HPV infection between groups. In the detection of CIN2+, the sensitivity, specificity, the PPV, NPV and the accuracy of PAX1 MS-HRM assay and high-risk HPV (HR-HPV) tests were respectively 80.6% vs 67.7%, 94.9% vs 54.5%, 83.3%, vs 31.8%, 94.0% vs 84.4%, and 91.5% vs 57.7%. The PAX1 MS-HRM assay proved superior to HR-HPV testing in the detection of high grade lesions (CIN2+) in ASC-H. This approach could screen out the majority of high grade lesion cases of ASC-H, and thus could reduce the referral rate to colposcopy.

• Mass Spectrometry Letters
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• 제6권3호
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• pp.53-58
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• 2015

Recombinant erythropoietins (EPOs) are an important class of biotherapeutics that stimulate red blood cell production. The quality, safety, and potency of EPO variants are determined largely by their glycosylation, which makes up nearly half their mass. Thus, detailed glycomic analyses are important to assess biotherapeutic quality and establish the equivalency of biosimilar EPOs now coming to market. High-resolution mass spectrometry (MS) has recently emerged as the premier tool for glycan analysis in EPOs. Using the accurate mass measurements provided by high-resolution MS, the compositions of even large, complex glycans can easily be determined. When combined with a nano-LC separation, differentiation of structural isomers also becomes a possibility. These components, together, provide a comprehensive picture of biotherapeutic glycosylation. In this review, we provide an overview of MS-based analytical platform for glycomic characterization of EPO biotherapeutics and biosimilars.

• Mass Spectrometry Letters
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• 제2권1호
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• pp.1-7
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• 2011

China's rapid economic growth has resulted in significant environmental side effects. Therefore, China has been interested in reducing her dependence on foreign oil and gas by developing technologies needed for hydrogen, in addition to her increasing energy mix of nuclear and renewable energy form, such as solar and wind power. There are three isotopes of hydrogen, i.e. protium (P or H), deuterium (D), and tritium (T). Both deuterium and tritium are important materials in nuclear fuel cycle industry. Tritium is one of the critical radioactive nuclides. Planning for and implementing contamination control as a part of normal operation and maintenance activities is an important function in any hydrogen facility, especially tritium facility. The development of hydrogen isotopes analysis is the key issues in this area. Mass spectrometry (MS) with medium (about 600) and high resolution (> 1,400) is commercially available; however, the routine analysis of hydrogen isotopes is done with low-resolution MS (< 200) in China. This paper summarizes the progress of MS measurement technology for hydrogen isotope abundance in China, focusing on our lab's research program and technical status. An analyzing method has been introduced for accurate measurement of tritium abundance in the H.D.T system by low resolution MAT-253 MS. The quotient of compression ratio coefficient is determined by building up equipment for laboratory-scale preparation of secondary standard gases and by considering the difference in sensitivity between hydrogen isotopes. The results show that the measured value is reproducible within the relative error range of 0.8% for gas samples of different tritium abundance.

• Mass Spectrometry Letters
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• 제1권1호
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• pp.13-16
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• 2010

The active components in a plant extract can be represented as mass profiles. We introduce here a new, multi-compound discovery method known as Scaling of Correlations between Activity and Mass Profiles (SCAMP). In this method, a correlation coefficient is used to quantify similarities between the extract activity and mass profiles. The method was evaluated by first measuring the anti-oxidation activity of eleven fractions of an Astragali Radix extract using DPPH assays. Next, 15 T Fouriertransform ion cyclotron resonance (FT-ICR) MS was employed to generate mass profiles of the eleven fractions. A comparison of correlation coefficients indicated two compounds at m/z 285.076 and 286.076 that were strong antioxidants. Principal component analyses of these profiles yielded the same result. FT-ICR MS, which offers a mass resolving power of 500,000, was used to discern isotopic fine structures and indicated that the molecular formula corresponding to the peak at m/z 285.076 was $C_{16}H_{13}O_5$. SCAMP in combination with high-resolution MS can be applied to any type of mixture to study pharmacological activity and is a powerful tool for active compound discovery in plant extract studies.

• Asian Journal of Atmospheric Environment
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• 제11권4호
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• pp.300-312
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• 2017

To improve the understanding of secondary organic aerosol (SOA) formation from the photo-oxidation of anthropogenic and biogenic precursors at the regional background station on Baengnyeong Island, Korea, gas phase and aerosol chemistries were investigated using the Proton Transfer Reaction Time of Flight Mass Spectrometer (PTR-ToF-MS) and the Aerodyne High Resolution Time of Flight Aerosol Mass Spectrometer (HR-ToF-AMS), respectively. HR-ToF-AMS measured fine particles ($PM_1$; diameter of particle matter less than $1{\mu}m$) at a 6-minute time resolution from February to November 2012, while PTR-ToF-MS was deployed during an intensive period from September 21 to 29, 2012. The one-minute time-resolution and high mass resolution (up to $4000m{\Delta}m^{-1}$) data from the PTR-ToF-MS provided the basis for calculations of the concentrations of anthropogenic and biogenic volatile organic compounds (BVOCs) including oxygenated VOCs (OVOCs). The dominant BVOCs from the site are isoprene (0.23 ppb), dimethyl sulphide (DMS, 0.20 ppb), and monoterpenes (0.38 ppb). Toluene (0.45 ppb) and benzene (0.32 ppb) accounted for the majority of anthropogenic VOCs (AVOCs). OVOCs including acetone (3.98 ppb), acetaldehyde (2.67 ppb), acetic acid (1.68 ppb), and formic acid (2.24 ppb) were measured. The OVOCs comprise approximately 75% of total measured VOCs, suggesting the occurrence of strong oxidation processes and/or long-range transported at the site. A strong photochemical aging and oxidation of the atmospheric pollutants were also observed in aerosol measured by HR-ToF-AMS, whereby a high $f_{44}:f_{43}$ value is shown for organic aerosols (OAs); however, relatively low $f_{44}:f_{43}$ values were observed when high concentrations of BVOCs and AVOCs were available, providing evidence of the formation of SOA from VOC precursors at the site. Overall, the results of this study revealed several different SOA formation mechanisms, and new particle formation and particle growth events were identified using the powerful tools scanning mobility particle sizer (SMPS), PTR-ToF-MS, and HR-ToF-AMS.