• Parasites, Hosts and Diseases
• /
• 제32권2호
• /
• pp.101-110
• /
• 1994

심장사상충 자충이 합성한 당단백의 분지(분지) 역할을 하는 N-linked high mannose 타입 올리고당의 구조에 대한 조사를 수행하였다. 사상충 자충을 방사선 표식 2-[$^3H$] mmannose를 함유한 배지에서 24시간 배양하였다. 자충으로부터 분리 정제한 당단백을 pronate로 소화시킨 다음. concanavalin A-Sepharose 크로마토그라피하여 분획하였다. 37%의 mannose가 자충 대사에 이용되었으며 pectin chromatography를 사용하여 high mannose 타입의 올리고당을 회수하였다. 이 올리고당을 $endo-{\beta}-N-acetylglucosaminidase$ H 효소로 소화시킨 후 HPLC를 사용하여 highmannose 타입 올리고당의 구성을 분석하였다. 심장사상충 자충이 합성한 high mannose 타입 올리고당의 형태는 $Man_5GIcNAc_2,{\;}Man_6GIcNAc_2,{\;}Man_7GIcNAc_2과{\;}Man_8GlcNAc_2$로 확인되었다. 이와 같이 심장사상충 자충이 체내 합성한 high mannose 타입 올리고당이 일반 척추동물이 체내 합성한 high mannose 타입 올리고당과 구조적으로 일치하였다.

• Bulletin of the Korean Chemical Society
• /
• 제35권3호
• /
• pp.955-957
• /
• 2014

• Parasites, Hosts and Diseases
• /
• 제42권2호
• /
• pp.57-60
• /
• 2004

A highly specific antigenic protein of 31 kDa from plerocercoid of Spirometra mansoni (sparganum) was obtained by gelatin affinity and Mono Q anion-exchange column chromatography. The purified 31 kDa protein was subjected to N-glycan enzymatic digestion for structural analysis. The relative electrophoretic mobility was analyzed by SDS-PAGE, before and after digestion. On SDS-PAGE after enzymatic digestion, the 31 kDa protein showed a molecular shift of approximately 2 kDa, which indicated the possession of complex N-linked oligosaccharides (N-glycosidase F sensitive) but not of high-mannose oligosaccharides (endo-beta-N-acetylglucosaminidase H, non-sensitive). Chemically periodated 31 kDa protein showed statistically non-significant changes with human sparganosis sera by enzyme linked immunosorbent assay (ELISA). Therefore, the dominant epitopes of the 31 kDa molecule in human sparganosis were found to be mainly polypeptide, while N-glycans of the antigenic molecule in sparganum was minimal in anti-carbohydrate antibody production.

• Journal of the Korean Wood Science and Technology
• /
• 제15권3호
• /
• pp.34-43
• /
• 1987

This study was performed to find out the types of linkage of carbohydrates in wood cell walls. To study the structure of linkage of carbohydrates in wood cell walls, we have attempted to find out the method holocellulose preparation and optimum condition of enzyme hydrolysis in holocellulose, and fractionate oligosaccharide with products that hydrolized partly by acetolysis and deacetylation in holocellulose. We have achieved four results. These results as follow; 1. At first. we reacted in wood meal $NaClO_2$ 1g per lignin lg for one hour and then the same of quantity $NaClO_2$ for four hours. Through these experiments, we have developed new holocellulose preparation method which had low loss of carbohydrates and high effect of the delignification. 2. The optimum condition of enzyme hydrolysis of holocellulose which had lignin was 0.005M sodium acetate buffer (pH 5.0). We have achieved 7.2% reducing sugar through the procedure that reactioned 0.01g holocellulose putting enzyme 0.03g for 72 hours. It may be supposed that 5.5% of lignin contained in holocellulose prevented enzyme contaction from holocellulose and so this lignin has resulted in the low efficiency of enzyme hydrolysis. 3. We did not fractionated from oligosaccharides which were preparated by the method of acetolysis and deacetylation in holocellulose. The reason is that holocellulose having a lot of lignin prevented prefectly partial hydrolysis from the method of acetolysis and deacetylation. 4. We attempted analysis of six standard substances through HPLC apparatus having sugar pak 1 column which we have changed flow rate and the column temperature variably. These six standard substances were D-glucose, D-mannose, D-xylose, D-galactose and L-rhamnose, L-arabinose, But sugar pak 1 column was not fitted analysis of four substances because D-galactose, D-mannose, D-xylose, L-rhamnose were agreement with elution time. And so, we could not analize four standard substances with sugar pak 1 column.

• 한국응용곤충학회지
• /
• 제22권4호
• /
• pp.293-299
• /
• 1983

강남콩 렉틴과 근류균(R. phaseoli)의 결합에 관한 광범위한 실험을 수행하여 다음과 같은 결과를 얻었다. 1. 강남콩종자의 자엽에서 가장 많은 렉틴은 성장에 따라 뿌리로 이동하였는데 발아후 약 5일경에, 뿌리의 길이가 $6\~8cm$인 것이 가장 렉틴의 함량이 많았다. 2. 근류균의 배양시기에 따가 강남콩렉틴과의 응집력을 측정 한 결과 배양초기 가 후기보다 응집력이 높았다. 3. 강낭콩종자에 존재하는 렉틴을 추출 정제하여서 6종 근류균과의 응집력을 측정한 결과 R. phaseoli와의 응집이 가장 강했고 이는 렉틴에 대한 특이적 결합, 즉 숙주특이성을 입증하는 것이었다. 4. 단당류에 의한 응집반응의 경쟁적 저해를 시도함으로써 강남콩렉틴의 근류균에 대한 결합부위가 mannose와 galactose를 포함한 oligosaccharides가는 것을 알수 있었으며 이들의 저해효과에 필요한 당의 최소농도는 6.25mM로서 측정되었다. 5. R. phaseoli와 강낭콩 렉틴을 강낭콩 뿌리에 인공감염시켜본 결과 렉틴의 경쟁적 저해가 확인되었으므로 뿌리혹형성에 렉틴의 가교가 관여한다는 가설을 입증할 수 있었다. 6. Immunodiffusion에 의한 R. phaseoli의 항원 결정기는 R. japonicum과 일부 관련성이 있을뿐 다른 Rhizobium spp.과는 전혀 다른 것으로 나타났다.