• 한국세라믹학회지
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• 제54권1호
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• pp.49-54
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• 2017

In this study, based on hydrolysis and condensation via $St{\ddot{o}}ber$ process of sol-gel method, synthesis of mono-dispersed silica nanoparticles was carried out with hydrophilic solvent. This operation was expected to be a more simplified process than that with organic solvent. Based on the sol-gel method, which involves simply controlling the particle size, the particle size of the synthesized silica specimens were ranged from 30 to 300 nm by controlling the composition of tetraethylorthosilicate (TEOS), DI water and ammonia solution, and by varying the stirring speeds while maintaining a fixed amount of ethanol. Increasing the content of DI water and decreasing the content of ammonia caused the particle size to decrease, while controlling the stirring speed at a high level of RPMs enabled a decrease of the particle size. X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR) were utilized to investigate the success factors for synthesizing process; Field emission scanning electron microscopy (FE-SEM) was used to study the effects of the size and morphology of the synthesized particles. To analyze the dispersion properties, zeta potential and particle size distribution (PSD) analyses were utilized.

• 전기전자학회논문지
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• 제10권2호통권19호
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• pp.103-108
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• 2006

본 논문에서는 상위 수준 합성에서 연산자들의 스위칭 최소화를 통한 저 전력 자원 할당 알고리즘을 제안했다. 본 논문에서는 이미 스케줄링 된 CDFG를 대상으로 전력 소모의 원인이 되는 스위칭 동작을 최소화하는 자원할당 알고리즘을 제안한다. 제안된 알고리즘은 DSP 분야의 회로나 필터를 대상으로 연산자가 소모하는 전력을 최소화 하고자 한다. 스케줄링 된 CDFG상에 있는 여러 개의 연산은 자원공유를 통하여 같은 기능 장치에 구현될 수 있다. 이런 경우 두 개의 연속적인 연산의 실행사이에 각 연산의 입력 변수들이 연속적으로 변화하기 때문에 기능장치의 스위칭동작이 변하게 된다. 이때 자원할당 과정에서 기능장치의 입력 신호들 사이의 스위칭동작과 상관관계를 고려하여 소비전력을 감소시킨다. 본 논문에서 제안하는 방법을 이용하여 자원할당을 할 경우 기존 방법과 비교했을 때 그 수행속도는 사용하는 연산자의 수와 최다 제어 단계에 따라서 빨라 질 수 있다. 그리고 소모하는 전력의 경우, 작게는 8.5%에서 9.3%까지 감소효과가 있다.

• Journal of Microbiology and Biotechnology
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• 제20권3호
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• pp.467-473
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• 2010

To improve the expression efficiency of recombinant endo-$\beta$-1,4-glucanase in P. pastoris, the endo-$\beta$-1,4-glucanase (egI) gene from Aspergillus niger was synthesized using optimized codons. Fourteen pairs of oligonucleotides with 15 bp overlap were designed and the full-length syn-egI gene was generated by two-step PCR-based DNA synthesis. In the synthesized endo-$\beta$-1,4-glucanase gene syn-egI, 193 nucleotides were changed, and the G+C content was decreased from 54% to 44.2%. The syn-egI gene was inserted into pPIC9K and transformed into P. pastoris GS115 by electroporation. The enzyme activity of recombinant P. pastoris stain 2-7# reached 20.3 U/ml with 1% barley $\beta$-glucan and 3.3 U/ml with 1% carboxymethylcellulose (CMC) as substrates in shake flasks versus 1,270.3 U/ml and 220.7 U/ml for the same substrates in 50-1 fermentors. The molecular mass of the recombinant protein was approximately 40 kDa as determined by SDS-PAGE analysis, the optimal temperature for recombinant enzyme activity was $70^{\circ}C$, and the optimal pH was 5.0 when CMC was used as the substrate.

• 한국항공우주학회지
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• 제44권3호
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• pp.274-280
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• 2016

2015년 발사예정인 우주기반기술 검증용 큐브위성 STEP Cube Lab.의 주요 탑재체인 열선절단방식 힌지구동형 구속분리장치는 높은 체결력 및 저충격 구현의 장점으로 초소형위성 전개구조물의 구속/분리에 적용이 용이하며, 기존 나일론선 절단방식의 낮은 체결력 및 복수구조물 적용에 따른 시스템 복잡화의 단점을 극복가능하다. 본 논문에서는 인증모델 검증시험이 완료된 구속분리장치의 궤도검증 임무수행을 목적으로 체결력 검증 및 전개 확인 신호 획득이 가능한 비행모델을 설계/제작하였으며, 탑재체 레벨에서의 발사 및 궤도환경 검증시험을 수행하여 설계 유효성을 입증하였다.

• 한국인터넷방송통신학회논문지
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• 제20권4호
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• pp.1-6
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• 2020

본 논문에서는 효율적인 AES 암호화를 위한 곱셈역원 연산기인 S-Box 설계를 제안한다. 제안한 방법은 먼저, 합성체 기반의 개선된 S-Box 모듈을 설계하고, 다단 파이프라인(multi-stage pipeline) 구조의 S-Box의 성능을 평가한다. 제안하는 S-Box 모듈에서의 곱셈역원 연산은 조합 논리로 구성되기 때문에 하드웨어 부담이 감소되고 처리 속도가 개선된다. 논리합성을 통하여 3-단 파이프라인 구조의 S-Box 의 경우, 기존 방법과의 연산속도 비교에서 약 28% 정도 개선됨을 보인다. 본 논문에서 제안한 개선된 S-Box는 Verilog-HDL을 사용하여 혼합 레벨에서 모델링을 행하였으며, Xilinx ISE 14.7툴을 사용하여 Spartan 3s1500l FPGA 상에서 합성을 수행하였다. 그리고 타이밍 시뮬레이션(ModelSim PE 10.3 사용)을 통하여 설계된 S-Box가 정상적으로 동작함을 확인하였다.

• KSBB Journal
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• 제18권4호
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• pp.294-300
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• 2003

본 연구에서는 L. fermentum KLB12을 열 전처리 함으로서 제제화 과정 동안 거치게 되는 열 스트레스에 대한 내성이 증진됨을 확인하고, 최적의 열 전처리 조건을 수행하였다. 또한 열 전처리 뿐만 아니라, 저온과 열 전처리 조건에도 열 스트레스에 대한 간섭 효과를 확인하였다. 그리고 내성 증진에 신규 단백질 합성이 필요함을 확인하였으며 나아가, 2-D electrophoresis를 통하여 7개의 신규 단백질을 확인하였다. 따라서 이 균주를 제제화하기 위한 방법으로 열 전처리를 이용할 경우 생균력 유지에 큰 효과를 얻을 수 있다고 사료된다.

• Journal of Nutrition and Health
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• 제55권1호
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• pp.70-84
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• 2022

Purpose: Skeletal muscles display significant heterogeneity in metabolic responses, owing to the composition of metabolically distinct fiber types. Recently, numerous studies have reported that in skeletal muscles, suppression of genes related to fatty acid channeling alters the triacylglycerol (TAG) synthesis and switches the energy substrates. However, such responses may differ, depending on the type of muscle fiber. Hence, we conducted in vitro and animal studies to compare the metabolic responses of different types of skeletal muscle fibers to the deficiency of fatty acyl-CoA synthetase (Acsl)6, one of the main fatty acid-activating enzymes. Methods: Differentiated skeletal myotubes were transfected with selected Acsl6 short interfering RNA (siRNA), and C57BL/6J mice were subjected to siRNA to induce Acsl6 deficiency. TAG accumulation and expression levels of insulin signaling proteins in response to acute glucose supplementation were measured in immortalized cell-based skeletal myotubes, oxidative muscles (OM), and glycolytic muscles (GM) derived from the animals. Results: Under conditions of high glucose supplementation, suppression of the Acsl6 gene resulted in decreased TAG and glycogen synthesis in the C2C12 skeletal myotubes. The expression of Glut4, a glucose transporter, was similarly downregulated. In the animal study, the level of TAG accumulation in OM was higher than levels determined in GM. However, a similar decrease in TAG accumulation was obtained in the two muscle types in response to Acsl6 suppression. Moreover, Acsl6 suppression enhanced the phosphorylation of insulin signaling proteins (Foxo-1, mTORc-1) only in GM, while no such changes were observed in OM. In addition, the induction ratio of phosphorylated proteins in response to glucose or Acsl6 suppression was significantly higher in GM than in OM. Conclusion: The results of this study demonstrate that Acsl6 differentially regulates the energy metabolism of skeletal muscles in response to glucose supplementation, thereby indicating that the fiber type or fiber composition of mixed muscles may skew the results of metabolic studies.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• 제2권3호
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• pp.210-218
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• 2021

Recently, pest-resistant living modified (LM) crops developed using RNA interference (RNAi) technology have been imported into South Korea. However, the potential adverse effects of unintentionally released RNAi-based LM crops on non-target species have not yet been reported. Coccinella septempunctata, which feeds on aphids, is an important natural enemy insect which can be exposed to the double-stranded RNA (dsRNA) produced by RNAi-based LM plants. To assess the risk of ingestion of Snf7 dsRNA by C. septempunctata, we first identified the species through morphological analysis of collected insects. A method for species identification at the gene level was developed using a specific C. septempunctata 12S rRNA. Furthermore, an experimental model was devised to assess the risk of Snf7 dsRNA ingestion in C. septempunctata. Snf7 dsRNA was mass-purified using an effective dsRNA synthesis method and its presence in C. septempunctata was confirmed after treatment with purified Snf7 dsRNA. Finally, the survival rate, development time, and dry weight of Snf7 dsRNA-treated C. septempunctata were compared with those of GFP and vATPase A dsRNA control treatments, and no risk was found. This study illustrates an effective Snf7 dsRNA synthesis method, as well as a high-concentration domestic insect risk assessment method which uses dsRNA to assess the risk of unintentional released of LM organisms against non-target species.

• Journal of Microbiology and Biotechnology
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• 제33권10호
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• pp.1337-1350
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• 2023

Caproic acid is a precursor substance for the synthesis of ethyl caproate, the main flavor substance of nongxiangxing baijiu liquor. In this study, Clostridium butyricum GD1-1, a strain with high caproic acid concentration (3.86 g/l), was isolated from the storage pit mud of nongxiangxing baijiu for sequencing and analysis. The strain's genome was 3,840,048 bp in length with 4,050 open reading frames. In addition, virulence factor annotation analysis showed C. butyricum GD1-1 to be safe at the genetic level. However, the annotation results using the Kyoto Encyclopedia of Genes and Genomes Automatic Annotation Server predicted a deficiency in the strain's synthesis of alanine, methionine, and biotin. These results were confirmed by essential nutrient factor validation experiments. Furthermore, the optimized medium conditions for caproic acid concentration by strain GD1-1 were (g/l): glucose 30, NaCl 5, yeast extract 10, peptone 10, beef paste 10, sodium acetate 11, L-cysteine 0.6, biotin 0.004, starch 2, and 2.0% ethanol. The optimized fermentation conditions for caproic acid production by C. butyricum GD1-1 on a single-factor basis were: 5% inoculum volume, 35℃, pH 7, and 90% loading volume. Under optimal conditions, the caproic acid concentration of strain GD1-1 reached 5.42 g/l, which was 1.40 times higher than the initial concentration. C. butyricum GD1-1 could be further used in caproic acid production, NXXB pit mud strengthening and maintenance, and artificial pit mud preparation.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제42권
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• pp.41.1-41.11
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• 2020

Background: Orthognathic surgery such as bilateral sagittal split ramus osteotomy (BSSRO) for the treatment of mandibular deformities is one of the most common procedures in maxillofacial operations that may lead to neurosensory disturbance. In this study, we aimed to evaluate the effectiveness of low-level laser therapy (LLLT) on augmenting recovery of neurosensory disturbance of inferior alveolar nerve (IAN) in patients who underwent BSSRO surgery. Methods: A comprehensive literature search was conducted by two independent authors in PubMed, Cochrane Central Register of Controlled Trials (CENTRAL), Scopus, Embase, and Google Scholar electronic databases. Besides, a manual search of all textbooks and relevant articles were conducted. Searches took place in August 2020 and were limited to published and peer-reviewed articles from 2000 to 2020. All analysis was performed using the comprehensive meta-analysis (CMA) and the STATA MP (version:16) software. The weighted mean difference (WMD) using the inverse variance method and the standard mean difference (SMD) was considered for continuous variables. Results: Seventy-four papers were retrieved after removing duplicate studies and finally, eight studies were assessed for qualitative synthesis and five for meta-analysis. Totally, 94 patients were included in the meta-analysis. Based on the meta-analysis, it was shown that LLLT was not effective in a short interval (0 to 48 h) after surgery, but in a period of more than 1 month after surgery, the positive results of treatment can be observed strikingly. Also, LLLT side/group showed no significant difference in some aspects of neurosensory recovery such as thermal sensation compared to the placebo side/group. Conclusions: The meta-analysis of randomized controlled trials revealed that LLLT generally improves IAN sensory disturbance caused by BSSRO. Further high-quality clinical trials with longer follow-up periods and larger sample sizes are recommended.