• Nuclear Engineering and Technology
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• v.55 no.10
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• pp.3549-3558
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• 2023

The jet pump can be used in a test device of a nuclear reactor for high flow amplification as it reduces inlet flow requirement and thereby size of the process components. In the present work, a miniature jet pump was designed to meet high flow amplification greater than 3. Subsequently, experiments were carried out using a test setup for design validation and performance evaluation of the jet pump for different parameters. It was observed that a minimum pressure of 0.6 bar (g) was required for the secondary fluid inside the jet pump to ensure cavitation free performance at high amplification. Spacing between the nozzle tip and the mixing chamber entry point had significant effect on the performance of the jet pump. Variation in primary flow, temperature and area ratio also affected the performance. It was observed that at high flow amplification, the analytical solution differed significantly from experimental results due to very large velocities encountered in the miniature size jet pump.

• The Plant Pathology Journal
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• v.38 no.2
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• pp.159-166
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• 2022

Barley yellow dwarf virus (BYDV) has been a major viral pathogen causing significant losses of cereal crops including oats worldwide. It spreads naturally through aphids, and a rapid, specific, and reliable diagnostic method is imperative for disease monitoring and management. Here, we established a rapid and reliable method for isothermal reverse transcription recombinase polymerase amplification (RT-RPA) combined with a lateral flow strips (LFS) assay for the detection of BYDV-infected oat samples based on the conserved sequences of the BYDV coat protein gene. Specific primers and a probe for RT-RPA reacted and optimally incubated at 42℃ for 10 min, and the end-labeled amplification products were visualized on LFS within 10 min. The RT-RPA-LFS assay showed no cross-reactivity with other major cereal viruses, including barley mild mosaic virus, barley yellow mosaic virus, and rice black streaked dwarf virus, indicating high specificity of the assay. The sensitivity of the RT-RPA-LFS assay was similar to that of reverse transcription polymerase chain reaction, and it was successfully validated to detect BYDV in oat samples from six different regions and in individual aphids. These results confirm the outstanding potential of the RT-RPA-LFS assay for rapid detection of BYDV.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2006.05a
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• pp.1285-1289
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• 2006

In a large diameter piping system, high frequency energy can produce excessive noise, high vibration, and failures of thermo-well, instrumentation, and attached small-bore piping. High frequency energy is generated by flow induced vibration like vortex shedding in orifices and valves. Once this energy is generated, amplification may occur from acoustical and/or structural resonances, resulting in high amplitude vibration and noise. At low frequencies, pipe vibration occurs laterally along the pipe's length, but at higher frequencies, the pipe shell wall vibrates radially across its cross-section. The simple beam analogy which is based on the beam mode vibration can not be applied to evaluate shell mode vibration. ASME OM3 recommends that the stress be measured directly by strain gauge and be evaluated according to the fatigue curves of the piping material. This Paper discusses the excitation and amplification mechanism relevant to high frequency energy generation in piping system, the monitoring method of the shell mode vibration in ASME OM3, the evaluation method generally used in the industry. Finally this paper presents the stress evaluation of the cavitating venturi down stream piping, where high frequency shell mode vibrations were observed during the operation.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2004.10a
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• pp.1282-1285
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• 2004

The high voltage driving system with multi-phase shifter including piezoelectric actuators comprised a driving power unit for outputting the driving power by converting input alternate current into direct current, a frequency shifting unit for supplying the direct current power and shifting or generating a frequency, a high-voltage amplification unit for amplifying the input signal outputted from the driving power unit and the frequency shifting unit into a high-voltage signal, and a phase shifting unit for shifting the phase difference of the amplified signal applied to the high-voltage amplification unit and driving plural piezoelectric actuators sequentially. The results that the operating voltage was stable, the voltage loss ratio was low and the response velocity was fast could be obtained. An experiment on performance of the high voltage driving system with multi-phase shifter designed and manufactured as above described was conducted by using a piezoelectric pump having 3 sheets of round unimorph piezoelectric actuators laminated respectively in a rectangular case. It sucks any fluid by causing the first piezoelectric actuator to shift from the inlet porter side, the phase delay of 60$^{\circ}$ causes the second piezoelectric actuator to begin to shift, and the phase delay of 120$^{\circ}$ causes the third piezoelectric actuator to begin to shift. As a result of measuring each change in the outlet flow rate of the piezoelectric pump, it was shown that the frequency-flow rate characteristic, the voltage-flow characteristic, and the load pressure-flow rate characteristic were improved.

• Fisheries and Aquatic Sciences
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• v.15 no.2
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• pp.107-115
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• 2012

In developing a useful tool to detect parasitic dynamics in an estuarine ecosystem, a denaturing high-performance liquid chromatography (DHPLC) assay was optimized by cloning plasmid DNA from the grass shrimp Palaemonetes pugio, and its two parasites, the trematode Microphallus turgidus and bopyrid isopod Probopyrus pandalicola. The optimal separation condition was an oven temperature of $57.9^{\circ}C$ and 62-68% of buffer B gradient at a flow rate of 0.45 mL/min. A peptide nucleic acid blocking probe was designed to clamp the amplification of the host gene, which increased the amplification efficiency of genes with low copy numbers. Using the DHPLC assay with wild-type genomic, the assay could detect GC Gram positive bacteria and the bopyrid isopod (P. pandalicola). Therefore, the DHPLC assay is an effective tool for surveying parasitic dynamics in an estuarine ecosystem.

• Parasites, Hosts and Diseases
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• v.59 no.2
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• pp.167-171
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• 2021

Haemonchosis remains a significant problem in small ruminants. In this study, the assay of recombinase polymerase amplification (RPA) combined with the lateral flow strip (LFS-RPA) was established for the rapid detection of Haemonchus contortus in goat feces. The assay used primers and a probe targeting a specific sequence in the ITS-2 gene. We compared the performance of the LFS-RPA assay to a PCR assay. The LFS-RPA had a detection limit of 10 fg DNA, which was 10 times less compared to the lowest detection limit obtained by PCR. Out of 24 goat fecal samples, LFS-RPA assay detected H. contortus DNA with 95.8% sensitivity, compared to PCR, 79.1% sensitivity. LFS-RPA assay did not detect DNA from other related helminth species and demonstrated an adequate tolerance to inhibitors present in the goat feces. Taken together, our results suggest that LFS-RPA assay had a high diagnostic accuracy for the rapid detection of H. contortus and merits further evaluation.

• The Plant Pathology Journal
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• v.39 no.5
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• pp.486-493
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• 2023

Cowpea mild mottle virus (CPMMV) is a global plant virus that poses a threat to the production and quality of legume crops. Early and accurate diagnosis is essential for effective managing CPMMV outbreaks. With the advancement in isothermal recombinase polymerase amplification and lateral flow strips technologies, more rapid and sensitive methods have become available for detecting this pathogen. In this study, we have developed a reverse transcription recombinase polymerase amplification combined with lateral flow strips (RT-RPA-LFS) method for the detection of CPMMV, specifically targeting the CPMMV coat protein (CP) gene. The RT-RPA-LFS assay only requires 20 min at 40℃ and demonstrates high specificity. Its detection limit was 10 copies/µl, which is approximately up to 100 times more sensitive than RT-PCR on agarose gel electrophoresis. The developed RT-RPA-LFS method offers a rapid, convenient, and sensitive approach for field detection of CPMMV, which contribute to controlling the spread of the virus.

• International Journal of Aeronautical and Space Sciences
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• v.7 no.2
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• pp.26-32
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• 2006

This paper targets a direct and quantitative prediction of characteristics of unstable waves in a combustion chamber, which employs the governing equations derived in terms of amplification factors of flow variables. A freshly formulated nonlinear acoustic equation is obtained and the analysis of unsteady waves in a rocket engine is attempted. In the present formalism, perturbation method decomposes the variables into time-averaged part that can be obtained easily and accurately and time-varying part which is assumed to be harmonic. Excluding the use of conventional spatially sinusoidal eigenfunctions, a direct numerical solution of wave equation replaces the initial spatial distribution of standing waves and forms the nonlinear space-averaged terms. Amplification factor is also calculated independently by the time rate of changes of fluctuating variables, and is no longer an explicit function for compulsory representation. Employing only the numerical computation, major assumptions inevitably inherent, and in erroneous manner, in up to date analytical methods could be avoided. With two definitions of amplification factor, 1-D stable wave and 3-D unstable wave are examined, and clearly demonstrated the potentiality of a suggested theoretical-numerical method of combustion instability.

• Journal of the Korean Institute of Telematics and Electronics D
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• v.35D no.4
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• pp.78-83
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• 1998

A small size cartrideg for FET type electrolyte sensor is designed and faricated with much simplified process by using micromachining tenchiques such as silicon etching andglass bonding. Size of the whole cartideg is 2.4cm*2.5cm, and the dead volume of a micro flow-channel in the cartrideg is only 8.5.mu.l. The photosensitive polymer(THB 30) is used to define a micropool and to encapsulate the sensor surface for standardizationof electrolyte sensors. To miniaturize micro flow-channel conventional reference electrode(Ag/AgCl) a differential amplification is introduced using REFET and quasi reference electrode. Refet was fabricated using photosensitive polymer(OMR 83). The fabricated cartridge with built-in pH-ISFET showed good operational characteristics such as linearity and high sensitivity (55.4mV/pH) in a wide pH range(pH2-pH12).

• Korean Journal of Optics and Photonics
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• v.1 no.1
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• pp.58-64
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• 1990

The flow direction in a glass tube is measured by using a forward scattering dual beam laser Doppler velo$.$ cimeter with an acousto-optic modulator. We can determine the flow direction by measuring the shifted Doppler frequency which is dependent on the order of modulation of the laser beam and the fluid flow direction. Also. an electronic amplification circuit which has a bandwidth of 0 . 300 MHz and a gain of 38 dB is designed and fabricated to amplify the high frequency signal.signal.