• 한국미생물·생명공학회지
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• 제23권1호
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• pp.60-67
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• 1995

Methanol assimilating yeast, Hansenula sp. MS-364 that has high productivity with methanol as carbon and energy source has been preserved at dept. of Microbiological engineering. Purification and properties of alcohol oxidase (E.C.1.1.3.13: oxygen oxidoreductase) were investigated in the methanol assimilating yeast, Hansenula sp. MS-364. Alcohol oxidase is related to the catalytic reaction that degrades alcohol to aldehyde and peroxide. The methanol oxidizing enzyme was purified by ammonium sulfate precipitation, DEAE-Sephadex A-50 chromatography and gel filtration on Sepharose 6B from cell-free extract. The purified enzyme preparation gave a single band in the sodium dodesyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The molecular weight of the enzyme was calculated to be about 576,000 and molecular weight of subunit was also calculated to be 72,000. The optimal pH and temperature of the enzyme reaction were pH 7.5 and 37$\circ$C, respectively. The enzyme was unstable in acidic pH and higher temperature. The enzyme was not specific for methanol and also oxidized lower primary alcohols. The Km value for methanol was 2.5 mM and that for ethanol was 1.66 mM. The enzyme was heavily inhibited by metal ions such as Hg$^{2+}$, Ag$^{2+}$, Cu$^{2+}$. The high concentration of EDTA and sulfhydryl reagents strongly inhibited the enzyme activity. The component of coenzyme was determined to flavin adenine dinucleotide.

• 천문학회지
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• 제42권6호
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• pp.145-153
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• 2009

We present measurements of diffuse interstellar $H_2$ absorption lines in the continuum spectra of 10 early-type stars. The data were observed with the Berkeley Extreme and Far-Ultraviolet Spectrometer (BEFS) of the ORFEUS telescope on board the ORFEUS-SPAS I and II space-shuttle missions in 1993 and 1996, respectively. The spectra extend from the interstellar cutoff at 912 $\AA$ to about 1200 $\AA$ with a resolution of ~ 3000 and statistical signal-to-noise ratios between 10 and 65. Adopting Doppler broadening velocities from high-resolution optical observations, we obtain the $H_2$ column densities of rotational levels J" = 0 through 5 for each line of sight. The kinetic temperatures derived from J" = 0 and 1 states show a small variation around the mean value of 80 K, except for the component toward HD 219188, which has a temperature of 211 K. Based on a synthetic interstellar cloud model described in our previous work, we derive the incident UV intensity IUV and the hydrogen density $n_H$ of the observed components to be -0.4 $\leq$ log $I_{UV}\leq2.2$ and $6.3{\leq}n_H2500cm^{-3}$, respectively.

• 천문학회지
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• 제38권2호
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• pp.219-222
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• 2005

We present the high-resolution (2"-4") images of the molecular envelopes surrounding the evolved stars, V Hya, VY CMa, and ${\pi}^1$ Gru observed with the Submillimeter Array. The CO J=2-1 and 3-2 images of the carbon star V Hya show that the circumstellar structure of this star consists of three kinematic components; there is a flattened disk-like envelope that is expanding with a velocity of ${\~}16 km\;s^{-1}$, the second component is the medium-velocity wind having a deprojected velocity of 40-120 km $s^{-l}$ moving along the disk plane, and the third one is the bipolar molecular jet having an extreme velocity of 70-185 km $s^{-l}$. The axis of this high velocity jet is perpendicular to the plane of the disk-like envelope. We found that the circumstellar structure of the S-star ${\pi}^1$ Gru traced by the CO J =2-1 resembles that of V Hya quite closely; the star is surrounded by the expanding disk-like envelope and is driving the medium-velocity wind along the disk plane. We also obtained the excellent images of VY CMa with the CO and $^{13}CO$ J=2-1 and $SO\;6_5-5_4$ lines. The maps of three molecular lines show that the envelope has a significant velocity gradient in the east-west direction, suggesting that the envelope surrounding VY CMa is also flattened and expanding along its radial direction. The high-resolution images obtained with the SMA show that some AGB stars are associated with the asymmetric mass loss including the equatorial wind and bipolar jet.

• Horticulture, Environment, and Biotechnology : HEB
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• 제59권6호
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• pp.875-888
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• 2018

Tulip (Tulipa L.) is one of the most important ornamental geophytes in the world. Analysis of molecular variability of tulips is of great importance in conservation and parental lines selection in breeding programs. Of the 70 genic microsatellites, 15 highly polymorphic and reproducible markers were used to assess the genetic diversity, structure, and relationships among 280 individuals of 36 wild and cultivated tulip accessions from two countries: Iran and the Netherlands. The mean values of gene diversity and polymorphism information content were 0.69 and 0.66, respectively, which indicated the high discriminatory power of markers. The calculated genetic diversity parameters were found to be the highest in wild T. systola Stapf (Derak region). Bayesian model-based STRU CTU RE analysis detected five gene pools for 36 germplasms which corresponded with morphological observations and traditional classifications. Based on analysis of molecular variance, to conserve wild genetic resources in some geographical locations, sampling should be performed from distant locations to achieve high diversity. The unweighted pair group method with arithmetic mean dendrogram and principal component analysis plot indicated that among wild tulips, T. systola and T. micheliana Hoog exhibited the closest relationships with cultivated tulips. Thus, it can be assumed that wild tulips from Iran and perhaps other Middle East countries played a role in the origin of T. gesneriana, which is likely a tulip species hybrid of unclear origin. In conclusion, due to the high genetic variability of wild tulips, they can be used in tulip breeding programs as a source of useful alleles related to resistance against stresses.

• 접착 및 계면
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• 제24권4호
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• pp.124-130
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• 2023

외부 온도 변화에 따른 특성변화가 적어야 하는 자동차 부품용 코팅제는 외부 환경 변화에 의한 노이즈 특성 변화가 매우 중요하다. 이에 내한성, 내후성, 내마모성이 우수한 연질의 폴리우레탄과 하이드록시 말단 혹은 곁사슬이 아민으로 변성된 폴리실록산 등이 널리 사용되고 있다. 본 연구에서는 각각의 폴리머 성분들이 노이즈에 미치는 영향을 확인하기 위하여 수분산 폴리우레탄을 합성하였고, 폴리실록산, ultra high molecular polypropylene (UHMWPP) 분말, 카본블랙, 소광제를 첨가하여 코팅제를 제조하였다. 수분산 폴리우레탄의 hard/soft 세그먼트의 함량을 27.1%/72.9%, 아민으로 변성된 폴리실록산과 하이드록시 말단 폴리실록산이 2:7의 비율에서 노이즈의 발생이 가장 적었으며, 마찰자가 고속에서 움직일 때 마찰계수의 차이가 컸다. 또한 SiO₂를 UHMWPP 분말로 대체함으로써, 노이즈가 감소하였으며 광택도 또한 감소하였다.

• Bulletin of the Korean Chemical Society
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• 제33권3호
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• pp.911-916
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• 2012

Ion selectivity in a simple cyclic peptide nanotube, composed of four cyclo[-(D-Ala-Glu-D-Ala-Gln)$_2-$] units, is investigated by calculating the PMF profiles of $Na^+$, $K^+$, and $Cl^-$ ions permeating through the peptide nanotube in water. The final PMF profiles of the ions obtained from the umbrella sampling (US) method show an excellent agreement with those from the thermodynamic integration (TI) method. The PMF profiles of $Na^+$ and $K^+$ display free energy wells while the PMF curve of $Cl^-$ features free energy barriers, indicating the selectivity of the cyclic peptide nanotube to cations. Decomposition of the total mean force into the contribution from each component in the system is also accomplished by using the TI method. The mean force decomposition profiles of $Na^+$ and $K^+$ demonstrate that the dehydration free energy barriers by water molecules near the channel entrance and inside the channel are completely compensated for by attractive electrostatic interactions between the cations and carbonyl oxygens in the nanotube. In the case of $Cl^-$, the dehydration free energy barriers are not eliminated by an interaction between the anion and the peptide nanotube, leading to the high free energy barriers in the PMF profile. Calculations of the coordination numbers of the ions with oxygen atoms pertaining to either water molecules or carbonyl groups in the peptide nanotube reveal that the stabilization of the cations in the midplane regions of the nanotube arises from the favorable interaction of the cations with the negatively charged carbonyl oxygens.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2003년도 추계학술대회 논문집 Vol.16
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• pp.108-111
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• 2003

Currently, molecular devices are reported utilizing active self-assembled monolayers containing the nitro group as the active component, which has active redox centers[1]. We confirm the electrical properties of 4,4-di(ethynylphenyl)-2'-nitro-1-benzenethiolate. To deposit the SAM layer onto gold electrode, we transfer the prefabricated Au(111) substrates into a 1mM self-assembly molecules in THF solution. Au(111) substrates were prepared by ion beam sputtering method of gold onto the silicon wafer. As a result, we measured current-voltage curve using ultra high vacuum scanning tunneling microscopy (UHV STM), I-V curve also clearly shows several current peaks between the negative bias region (-0.3958V) and the positive bias region (0.4658V), respectively.

• 대한화학회지
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• 제63권2호
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• pp.78-84
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• 2019

High-resolution $^1H$ NMR spectroscopic technique has been widely used as one of the most powerful analytical tools in food chemistry as well as to define molecular structure. The $^1H$ NMR spectra-based metabolomics has focused on classification and chemometric analysis of complex mixtures. The principal component analysis (PCA), an unsupervised clustering method and used to reduce the dimensionality of multivariate data, facilitates direct peak quantitation and pattern recognition. Using a combination of these techniques, the various green teas and black teas brewed were investigated via metabolite profiling. These teas were characterized based on the leaf size and country of cultivation, respectively.

• 한국육종학회지
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• 제43권5호
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• pp.479-489
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• 2011

Gluten is the main functional component of wheat, and is the main source of the viscoelastic properties in a dough. One of the gluten group is glutenin, which is composed of high molecular weight (HMW) and low molecular weight (LMW) subunits. The HMW glutenin subunits (HMW-GS) have been shown to play a crucial role in determining the processing properties of the grain. They are encoded by the Glu-1 loci located on the long arms of homeologous group one chromosomes, with each locus comprising two genes encoding x- and y-type subunits. The presence of certain HMW subunits is positively correlated with good bread-making quality. The highly conserved N- and C- terminal contaning cystein residues which form interand intra-chain disulphide bonds. This inter chain disulphide bonds stabilize the glutenin polymers. In contrast, the repetitive domains that comprise the central part of the HMW-GS are responsible for the elastic properties due to extensive arrays of interchain hydrogen bonds. In this review, we discuss HMW-GS, HMW-GS structure and gluten elasticity, relationship between HMW-GS and bread wheat quality and genetic engineering of the HMW-GS.

• 천문학회지
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• 제38권2호
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• pp.249-252
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• 2005

We present results of the velocity-resolved spectroscopy of the [Fe II] $\lambda$1.644${\mu}m$ emission toward outflow sources with the Subaru Telescope at the angular resolution of 0.apos;16 ${\~}$ 0.apos;5 arcseconds. The observed sources are L1551 IRS 5, DG Tau, HL Tau and RW Aur, which are located in the Taurus-Aurigae Molecular Cloud, one of the closest star forming regions (0.apos;1 = 14 AU). We were able to resolve outflow structure in the vicinity of the sources at a scale of a few tens of AU. The position-velocity diagram of each object shows two velocity components: the high velocity component (HVC: 200 - 400 km $s^{-l}$) and the low velocity component (LVC: 50 - 150 km $s^{-l}$), which are clearly distinct in space and velocity. The HVC may be a highly collimated jet presumed from its narrow velocity width and high velocity. The LVC, on the other hand, may be a widely opened disk wind inferred from its broad velocity width and low velocity. The spectrum taken perpendicular to the L1551 IRS 5 outflow at its base shows that the LVC has a spatially wide subcomponent, supporting the above interpretation. We demonstrated that the [Fe II] 1.644 $\mu$ spectroscopy is a very powerful tool for the studies of fast jets and winds that directly emanate from star-disk systems.