• Journal of Broadcast Engineering
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• v.20 no.3
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• pp.408-413
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• 2015

Recently, According to increasing interest to original sound Karaoke instrument, MIDI type karaoke manufacturer attempt to make more cheap method instead of original recoding method. The specific method is to make the original sound accompaniment to remove only the voice of the singer in the singer music album. In this paper, a system to separate vocal components from music accompaniment for stereo recordings were proposed. Proposed system consists of two stages. The first stage is a vocal detection. This stage classifies an input into vocal and non vocal portions by using SVM with MFCC. In the second stage, selective frequency subtractions were performed at each frequency bin in vocal portions. In this case, it is determined in consideration not only the energies for each frequency bin but also the phase of the each frequency bin at each channel signal. Listening test with removed vocal music from proposed system show relatively high satisfactory level.

• Transactions of the KSME C: Technology and Education
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• v.2 no.1
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• pp.1-8
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• 2014

Recently vehicle development trend changes from machinery vehicle to the electrical and electronic devices. As well the electrical and electronic devices commercial vehicles are increasing more than machinery system. The capacitance fuel sensor is a sensor that, by measuring the capacitance value of fuel level sensor MCU operating for the final voltage value of the signal output. That is increasing durability and fuel measurement accuracy more than conventional ceramic resistor type. For Sensor software verification R-BENCH TOOL generated test case automatically. We make the programmable Capacitor board for test. And it was confirmed that more than 98% of the high reliability of the software.

• Journal of the Korea Society of Computer and Information
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• v.16 no.2
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• pp.17-23
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• 2011

In this paper, daily life stress monitoring system is proposed. The proposed wireless ECG module, reducing the noise and increasing the size of signal, amplification circuit was designed for. Using HRV(Heart Rate Variability), extracted by measuring R-wave, stress diagnostic algorithms to assess the stress of human emotion were developed. For monitoring the activities, the proposed system is consist of small rectangular size for portable and by simple measurement it is possible to measure at any time. Through experiments, the proposed approach to represent user's stress level can be confirmed. Through that, it can see appropriate structure to obtain R-wave for stress assess as well as high resemblance to the clinical electrocardiogram. In this paper, performed experiments was developed nonrestraint measuring and wearable wireless biometric scanner that is able to monitor the heart's electrical activity of everyday life.Using this, the algorithm system, that is able to assess stress index through time-domain and frequency-domain analysis of the front and the rear of performing stress load protocol, was developed,

• The Korean Journal of Food And Nutrition
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• v.10 no.2
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• pp.180-185
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• 1997

Sikye was produced from glutinous rice. The glutinous rice Sikhye was found to contain 7.3% of limit dextrin, 10.1% of maltose, 1.3% of maltotriose and 1.75% of rice residue. Limit dextrin in glutinous rice Sikhye was purified by ethanol fractionation followed by gel chromatography on Biogel P-2. The purified limit dextrin showed both signal of $\alpha$-1,4- and $\alpha$-1,6-glucosidic linkage with its estimation ratio of 5:1 by 1H-NMR analysis. Limit dextrin was digested with enzymes(30units/ml) of $\alpha$-amylase, $\alpha$-glucosidase and glucoamylase from Aspergillus awamori, sweet potato $\beta$-amylase and human salivary $\alpha$-amylase at 37$^{\circ}C$ for 1 hour, respectively. Hydrolysis rates of these amylases on it were similar that of rice Sikhye. $\alpha$-Glucosidase plus human salivary $\alpha$-amylase hydrolyzed it to 18%. The results suggest that glutinous rice is more effective to produce high level of branched maltooligosaccharide compared with rice as raw material for Sikye making.

• Journal of the Institute of Electronics and Information Engineers
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• v.49 no.10
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• pp.54-59
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• 2012

Multiple antenna transmission and reception, whose principal merits are significant increase in spectral efficiency and/or reduction in error rate, lose much of their effectiveness in high levels of interference from other cells. Incorporating the other cell interference into advanced signal processing at transmitter and receiver is one of the key challenges for cell boundary users in cellular system. Since receiver can obtain exact knowledge of interference channels more easily than transmitter, an interference-aware multiple antenna receiver that can significantly attenuate interferences is considered. Based on the receiver, codebook-based coordinated precoding schemes are proposed. According to the level of cooperation, centralized and distributed schemes are proposed. We verified by the simulation results that even the distributed schemes, which have same amount of feedback and no cooperation between cells, have performance gain compared to the conventional non-coordinated scheme.

• The Journal of the Korea Contents Association
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• v.10 no.3
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• pp.64-71
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• 2010

Recently, to achieve high performance of the processor, the cache is splits physically into two parts, one for instruction and one for data. This paper proposes an architecture of asynchronous instruction cache based on mixed-delay model that are DI(delay-insensitive) model for cache hit and Bundled delay model for cache miss. We synthesized the instruction cache at gate-level and constructed a test platform with 32-bit embedded processor EISC to evaluate performance. The cache communicates with the main memory and CPU using 4-phase hand-shake protocol. It has a 8-KB, 4-way set associative memory that employs Pseudo-LRU replacement algorithm. As the results, the designed cache shows 99% cache hit ratio and reduced latency to 68% tested on the platform with MI bench mark programs.

• Ocean and Polar Research
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• v.25 no.4
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• pp.607-615
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• 2003

Many organisms are known to survive in icy environments. These include both over wintering terrestrial insects and plants as well the marine fish inhabiting high latitudes. The adaptation of these organisms is both a fascinating and important topic in biology. Marine teleosts in particular, can encounter ice-laden seawater that is approximately $1^{\circ}C$ colder than the colligative freezing point of their body fluids. These animals produce a unique group of proteins, the antifreeze proteins (AFPs) or antifreeze glycoproteins (AFGPs) that absorb the ice nuclei and prevent ice crystal growth. Presently, there are at least four different AFP types and one AFGP type that are isolated from a wide variety of fish. Despite their functional similarity, there is no apparent common protein homology or ice-binding motifs among these proteins, except that the surface-surface complementarity between the protein and ice are important for binding. The remarkable diversity of these proteins and their odd phylogenetic distribution would suggest that these proteins might have evolved recently in response to sea level glaciations just 1-2 million years ago in the northern hemisphere and 10-30 million years ago around Antarctica. Winter flounder, Pleuronectes americanus, has been used as a popular model to study the regulation of AFP gene expression. It has a built-in annual cycle of AFP expression controlled negatively by the growth hormone. The signal transduction pathways, transcription factors and promoter elements involved in this process have been studied in our laboratory and these studies will be presented.

• Journal of Microbiology and Biotechnology
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• v.9 no.6
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• pp.811-819
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• 1999

The ${\beta}-CGTase$ gene of alkalophilic Bacillus firmus var. alkalophilus was cloned into E. coli using $pZErO^{TM}-2$ as a vector. The cloned gene encoded a total of 710 amino acid residues consisting of 674 amino acids of the matured protein and 36 amino acids of the signal peptide, including 20 amino acids from the lacZ gene in the vector. Although the cloned ${\beta}-CGTase$ gene did not contain the promoter and start codons, it was expressed by the lac promoter and lacZ start codon in the $pZErO^{TM}$ vector. A comparison was made with the amino acid sequence and ten other CGTases from Bacillus sp. Also, ten highly conserved regions, which are important amino acid residues in catalysis of CGTase, were identified. The lac promoter used for expression of the ${\beta}-CGTase$ gene was induced constitutively in recombinant E. coli even without IPTG possibly because of a lack of the lacI gene in both host and vector, repressing the lacZ gene in the lac operon. Its expression was catabolically repressed by glucose, however, its repression was reduced by soluble starch, mainly because of the extremely high increase of the cAMP level. ${\beta}-CGTase$ can be overproduced in the recombinant E. coli by maintaining intracellular cAMP levels mostly through the intermittent feeding of glucose during cultivation.

• Journal of Microbiology and Biotechnology
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• v.15 no.1
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• pp.59-65
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• 2005

Poly-${\gamma}$-glutamic acid (PGA) is an unusual anionic polypeptide and has great potential as an environmentally and industrially significant biodegradable material. A new ${\gamma}$-PGA producer, Bacillus mesentericus MJM1, with high production capacity was isolated from Korean domestic Chungkuckjang bean paste. It produced ${\gamma}$-PGA at the level of 10 g/l in suitable media. The viscosities of 5% initially extracted mucin and purified ${\gamma}$-PGA solutions were 660 cps and 600 cps, respectively. The produced ${\gamma}$-PGA polymer consisted of 2,000 glutamic acid residues with even proportion of L and D types with molecular mass of about 200- 300 kDa. Bacillus mesentericus MJM1 displayed ${\gamma}$-glutamyltranspeptidase (${\gamma}$-GTP) activity that is known to play a key role in ${\gamma}$-PGA biosynthesis. The ${\gamma}$-GTP coding region was located on the plasmid of 5.8 kb. The plasmid, named pMMH1, is a rolling-circle replication (RCR) plasmid and additionally contained a replication origin and type I signal peptidase (sipP) coding region.

• Biomedical Science Letters
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• v.11 no.2
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• pp.135-141
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• 2005

A Jopock (Jpk), a trans-acting factor associating with the position-specific regulatory element of murine Hoxa-7, has shown to have a toxicity to both prokaryotic and eukaryotic cells when overexpressed. Since Jpk protein harbors a transmembrane domain and a putative endoplasmic reticulum (ER)-retention signal at the N-terminus, a subcellular localization of the protein was analyzed after fusing it into the green fluorescent protein (GFP): Both N-term (Jpk-EGFP) and C-term tagged-Jpk (EGFP-Jpk) showed to be localized in the ER when analyzed under the fluorescence microscopy after staining the cells with ER- and MitoTracker. Since ER stress triggers the ER-stress mediated apoptosis to eliminate the damaged cells, we analyzed the expression pattern of Jpk under ER-stress condition. When MCF7 cells were treated with the ER-stress inducer such as DTT and EGTA, the expression of Jpk was upregulated at the transcriptional level like that of Grp78, a molecular chaperone well known to be overexpressed under ER-stress condition. In the presence of high concentration of ER-sterss inducer (10 mM), about 70 (DTT) to $95\%$ (EGTA) of cells died stronly expressing ($10\~12$ fold) Jpk. Whereas at the low concentration ($0.001\~1.0\;mM$) of the inducer, the expression of Jpk was increased about 2.5 (EGTA) to 5 fold (DTT), which is rather similar to those of ER chaperone protein Grp78. These results altogether indicate that the ER-stress upregulated the expression of Jpk and the excess stress induces the ER-stress induced apoptosis and the concomitant expression of Jpk.