• 대한한방소아과학회지
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• 제38권1호
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• pp.37-45
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• 2024

Objectives The aim of this study was to determine the inhibitory effects of Bangpungtongsungsan extract (BTS) on fat accumulation in high-fat diet-induced young obese mice. Methods The extract was administered to 3-week-old C57BL/6 male mice fed with a high-fat diet. The experimental groups were divided into a control group (Ctrl), high-fat diet group (HFDF), and BTS treated group after high fat diet feeding (BTST), with 10 mice assigned to each group. Lipid synthesis was observed to confirm the inhibition of fat synthesis. Changes in body weight, body fat percentage, and total cholesterol in the blood were observed to confirm weight control. Peroxisome proliferator-activated receptor gamma (PPAR-γ) and sterol regulatory element-binding protein (SREBP)-1 positivity was observed to confirm the inhibition of fat accumulation in liver tissue. Results Bangpungtongsungsan significantly inhibited lipid synthesis. Changes in body weight, body fat percentage, and total cholesterol in the blood were significantly lower in BTST rats than in HFDF rats. PPAR-γ and SREBP-1 positivity were significantly lower in BTST rats compared to HFDF rats. Conclusions This study confirms the potential of BTST to inhibit fat accumulation in obesity.

• 한국지역사회생활과학회지
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• 제12권1호
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• pp.15-22
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• 2001

This study was conducted to find out the effect of fermented milk on lowering of serum and liver cholesterol in high diet. Sprague-Dawley male rats of 200g initial average weight were assigned to four experimental group : (1) high fat diet + milk (2) high fat diet + bifidobacteria (3) high fat diet + microcapsulated bifidobacteria (4) high fat diet + fermented milk by bifidobacteria. Milk, bifidobacteria, microcapsulated bifidobacteria and fermented milk by bifidobacteria were given 2ml per day. According to the result, there was lowering effect of serum and liver total cholesterol in fermented milk feeding group, Serum LDL-cholesterol was also low tendency in fermented milk by bifidobacteria feeding group. Accordingly, it was confirmed that the material that caused hypocholesteremic effect was not the bifidobacteria itself in fermented milk but the metabolic material produced in the fermentation, and it inhibited cholesterol synthesis in liver.

• Journal of Nutrition and Health
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• 제31권8호
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• pp.1217-1225
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• 1998

This study was conducted to investigate the effects of shellfish on lipid metabolism in rats fed high fat supplements. Male sprague-dawley rats weighting approximately 165g were fed a basal diet, a high fat diet, or a high fat diet plus shellfish for 4 weeks. The shellfishes on the were oyster, hard-shelled mussel, little neck clam, and march clam. Alfter 4 weeks high fat diet, supplementation of 20% lard significantly increased plasma. GOT, GPT, ${\gamma}$-GTP , and liver triglyceride (TG). Plasma GOT, GPT, ${\gamma}$-GTP , triglyceride, and total cholestrerol levels were significantly lower in shellfish groups than in basal and high-fat groups regardless of high-fat supplementation (p<0.05). The total lipid and cholesterol content in liver showed similar results(p<0.05). There were no differences in glucose, HDL-cholesterol in plasma and total cholesterol and total lipid in liver between basal and high-fat supplemented diets. Liong chain fatty acids, specific components of shellfishes group, were exclusively higher than in basal and high-fat diets, and were most well-reflected in liver and plasma. From the above results, the hypolipidemic effects of shellfish were detected in the process of inducing hyperlipidemia by high-fat supplement.

• Journal of Nutrition and Health
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• 제27권8호
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• pp.785-794
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• 1994

The effect of feeding various diets on plasma lipids, liver lipids, fecal excretion of lipids and triglyceride secretion rate was studied in Sprague-Dawley rats fed for 1 month diets containing high fat(40% of energy ; HF) high cholesterol(1% ; HC), low fat/high sucrose(70% ; HS) or control diet(CT). Weight gain in HS group but there were no differences in TG contents of HF or HC groups compared to CT group. Fecal excretion of lipid was higher in HC than in HF, HS and CT groups. Hepatic TG secretion was not significantly different in four dietary groups. Accordingly, hypertriglyceridemia shown in HS group does not seem to be resulted from the increased TG secretion rate.

• Journal of Ginseng Research
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• 제29권3호
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• pp.138-144
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• 2005

본 연구에서는 high fat diet에 대한 인삼 추출물의 항산화 효과를 알아보기 위해 실험동물로 생쥐를 이용하였다. 대조군은 일반사료만을, FD군은 high fat diet만을 먹였으며, 실험군은 high fat diet와 인삼추출물을 경구 투여한 후 간 조직에서의 SOD, catalase, GPS의 활성 및 MDA 및 $H_2O_2$ 함량 등을 측정함으로서 fat diet독성에 대한 지질과산화와의 상호 관련성, 인삼의 항산화 효소의 효과를 비교 검토하였다. Fat diet 독성에 대한 항산화 효소인 SOD의 활성은 인삼 추출물 군에서 전반적으로 낮았으며, GPx의 활성은 FR500, FR3000군에서 가장 높은 결과를 보였다. 과산화수소 함량은 FD군에서 약간 높았지만, 모든 군에서 거의 유사한 결과를 보였다. 자유라디칼에 의해 생성된 지질과산화의 최종 산물인 MDA의 함량은 FD군을 기준으로 인삼추출물 군에서 모두 낮은 수치를 보였다. 이와 같이 fat diet에 대한 인삼 추출물은 SOD의 활성 증대보다는 자유라디칼 제거제로서 항산화 효과를 보였다고 생각하고, GPx의 직접적인 작용과 생체 내에서 내인성 항산화 물질인 GSH의 합성 능력을 강화시킴으로서 산화적 손상에 대한 방어기전을 향상시키는 결과라고 생각한다.

• International Journal of Industrial Entomology and Biomaterials
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• 제22권2호
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• pp.95-100
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• 2011

The effect of diet supplementation of Antheraea pernyi (A. pernyi) silk fibroin on the lipid metabolism and antioxidant defense status in high fat-fed mice was investigated. The animals were given normal control diet (NC group), high fat diet (HF group), or high fat diet supplemented with A. pernyi silk fibroin powder (HFS group) for 7 weeks. After the experimental period, the HF group showed significant increase in body weight, plasma and hepatic total cholesterol levels, and hepatic triglyceride concentration, and decreased activities of hepatic antioxidant enzymes relative to NC group. However, the HFS group exhibited marked reduction in body weight, plasma cholesterol and hepatic triglyceride levels, hepatic lipogenic enzyme activities, and lipid peroxidation rate, and higher high-density lipoprotein (HDL)-cholesterol level, fecal triglyceride content, and antioxidant enzymes activities compared with that of HF group. These findings demonstrate that dietary feeding of A. pernyi silk fibroin could improve the lipid metabolism and antioxidant defense system via regulation of hepatic antioxidant and lipogenic enzymes activities. Hence, this silk fibroin may be beneficial as a functional biomaterial for the development of therapeutic agent against high fat diet-induced hyperlipidemia and its related diseases.

• 생명과학회지
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• 제14권4호
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• pp.531-536
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• 2004

신령버섯 균사체 액체배양액이 고콜레스테롤 식이를 섭취한 흰쥐의 지질대사에 미치는 영향을 조사하고자, 생후 7주령의 숫쥐에 표준식이를 급여한 정상군, 표준식이에 15% 돈지와 0.5% 콜레스테롤을 첨가한 식이를 급여한 대조군, 대조식이에 신령버섯 균사체 액체배양액을 음료수에 30% 및 40%로 혼합 급여한 군(30% 및 40% 신령버섯군) 등 4군으로 나누어 6주간 사육한 결과는 다음과 같다. 실험동물의 체중 증가량, 식이섭취량 및 식이효율은 대조군과 30%와 40% 신령버섯군이 유사하였다. 간, 신장 및 부고환지방의 무게는 대조군과 신령버섯군들이 비슷하였다 간의 콜레스테롤 농도, 간과 혈청의 중성지질 농도는 대조군과 신령버섯군들이 비슷한 수준으로 감소효과가 나타나지 않았다. 혈청의 총 콜레스테롤과 LDL-콜레스테롤 농도 및 동맥경화지수는 대조군에 비해 30% 및 40% 신령버섯군이 유의하게 감소되었다. 총콜레스테롤에 대한 HDL-콜레스테롤의 비율은 대조군에 비해 신령버섯군들이 유의하게 증가되었다. HDL-콜레스테롤 농도는 대조군과 신령 버섯군들이 비슷한 수준으로 신령 버섯섭취에 따른 영향은 나타나지 않았다. 분으로 배설된 지방은 대조군에 비해 40% 신령버섯군이 유의하게 증가되었다. 이상의 결과로 보아 고콜레스테롤 식이를 급여한 흰쥐에 신령버섯 균사체 액체배양액을 30%나 40% 급여시 혈청의 총 콜레스테롤, LDL-콜레스테롤 농도 및 동맥경화지수를 낮추고, 총 콜레스테롤에 대한 HDL-콜레스테롤의 비율을 증가시키는 것으로 나타났다.

• Food Science and Biotechnology
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• 제18권1호
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• pp.197-201
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• 2009

This study evaluated the effect of biocellulose and the diet formulation on reducing body weight gain of obese rats induced by high-fat diet for 10 weeks. Thirty male Sprague-Dawley rats were randomly assigned to high-fat diet group (OB-CON), high-fat diet group containing 5% biocellulose (OB-BIO), and high-fat diet group containing 5% dietary formulation (OB-DF). After feeding each diet for additional 10 weeks, body weight gains of OB-BIO and OB-DF groups were lower by 3.3 and 4.8%, respectively as compared with that of OB-CON group. Although not significant, measured values of the perirenal and visceral fat pads of OB-BIO and OB-DF groups were lower than those of the OB-CON group. The weight of interscapular brown adipose tissue did not show significant difference in all groups. The size of adipocyte in rats was lower in both OB-BIO and OB-DF groups. Thus, biocelluose and the diet formulation showed the anti-obesity effect.

• 운동영양학회지
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• 제13권2호
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• pp.93-100
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• 2009

This study was conducted to investigate the effect of exercise training on blood and metabolic variances and genes expressions in hyperlipidemic rats. Three weeks-old male rats were randomly assigned into chow (n=7), high-fat diet (HF, n=7) and HF+exercise (HF+EX, n=7) groups. Exercise training consisted of the treadmill running 5 times per week during 8 weeks (0% grade, 30 min/time for first 4 weeks and 0% grade, 60 min/time the other 4weeks). The levels of triglyceride and total -cholesterol were increased in HF diet compared with chow group, and recovered to level of chow group by exercise training. Plasma glucose and insulin concentrations increased by 40 and 50%, respectively in HF diet compared with chow diet group, and these increases returned to the level of chow group by exercise training (p<.05). Body weight and abdominal fat mass were increased by high-fat diet compared with chow diet, and recovered to level of chow group by exercise training. Long-chain fatty acid oxidation rate and AMPK protein expression was not changed by HF diet, but increased by exercise training compared with high-fat diet (p<.05). UCP3 protein expression was not changed by either high-fat diet or exercise training compared with chow group. There was high correlation between plasma triglyceride and total cholesterol concentrations(p<.01). Plasma triglyceride or total cholesterol level showed correlation with following factors; plasma insulin and glucose levels, body weight, abdominal fat weight, UCP3 protein expression and long-chain fatty acid oxidation rate. These results showed that exercise training on the treadmill recovered hyperlipidemia, hyperglycemia and hyperinsulinemia induced by high-fat diet for 8 weeks. These exercise effects may be related with decreased body weight and abdominal fat mass, and increased long-chain fatty acid oxidation rate.

• Journal of Nutrition and Health
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• 제40권8호
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• pp.693-700
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• 2007

This study was performed to investigate the effects of genistein, a kind of soy isoflavones, on fatty liver and lipid metabolism in rats fed high fat diet. Twenty four male Sprague-Dawley rats were divided into four groups by dietary fat and genistein contents then raised for six weeks. The rats(n=6/group) were fed normal fat diet(NOR), high fat diet (HF), high fat with 0.1% genistein(HF+0.1%G) or high fat with 0.2% genistein(HF+0.2%G). Hepatic total lipid, triglyceride, total cholesterol and Serum GPT, as a marker for fatty liver, were significantly increased by high fat diet. Also, serum total lipid, triglyceride, total cholesterol, glucose and insulin concentration, hepatic lipogenic enzyme (fatty acid synthase and malic enzyme) activities were significantly increased by high fat diet. However, hepatic total lipid, triglyceride, total cholesterol and Serum GPT were significantly decreased by genistein intake. Also, genistein supplementation decreased serum total lipid, triglyceride, glucose and insulin concentration, hepatic lipogenic enzyme (fatty acid synthase and malic enzyme) activities. There were no differences by genistein level except for serum insulin. These results suggest that fatty liver induced by high fat diet was caused by increased serum lipid profiles and hepatic lipogenesis, whereas, genistein may be useful in inhibiting of fatty liver by reducing serum lipid profiles and hepatic lipogenesis.