• BMB Reports
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• v.37 no.3
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• pp.275-281
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• 2004

Rhodanese was isolated and purified from the cytosolic fraction of liver tissue homogenate of the fruit bat, Eidolon helvum, by using ammonium sulphate precipitation and CM-Sephadex C-50 ion exchange chromatography. The specific activity was increased 130-fold with a 53% recovery. The $K_m$ values for KCN and $Na_2S_2O_3$ as substrates were $13.5{\pm}2.2\;mM$ and $19.5{\pm}0.7\;mM$, respectively. The apparent molecular weight was estimated by gel filtration on a Sephadex G-100 column to be 36,000 Da. The optimal activity was found at a high pH (pH 9.0) and the temperature optimum was $35^{\circ}C$. An Arrhenius plot of the heat stability data consisted of two linear segments with a break occurring at $35^{\circ}C$. The apparent activation energy values from these slopes were 11.5 kcal/mol and 76.6 kcal/mol. Inhibition studies on the enzyme with a number of cations showed that $Mg^{2+}$, $Mn^{2+}$, $Ca^{2+}$, and $Co^{2+}$ did not affect the activity of the enzyme, but $Hg^{2+}$ and $Ba^{2+}$ inhibited the enzyme.

• Journal of the Korean Society of School Health
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• v.21 no.2
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• pp.89-95
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• 2008

Purpose: The purpose of this study is to investigate the heavy metal pollution of playgrounds of elementary schools in the Ulsan Metropolitan City. Soil pollution is one of the most dangerous problems of the utmost concern to both children and teachers. The result of this study would be an educational datum for improving children's health and establishing educational policy. Methods: For the analysis, fifteen representative schools were selected from five sample areas such as Dong-gu, Nam-gu, Jung-gu, Buk-gu and Ulju-gun of the Ulsan city. And from each school playground, soil was extracted from five spots and mingled into one sample. These fifteen samples were pretreated by the microwave extraction method, and the extracted heavy metals(As, Cd, Cu, Pb, Cr⁶⁺, Zn and Hg) were quantified by the ICP(Inductively Coupled Plasma) system. Results: The results of the analysis shows that the level of heavy metal pollution of elementary school playgrounds in Ulsan city did not exceed the permitted limit of each heavy metal. This means that the soil pollution of playgrounds in the Ulsan Metropolitan City is not so serious as it is expected. But it should be added that the soil is not too safe to be ignored.

• Journal of Chest Surgery
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• v.24 no.5
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• pp.445-450
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• 1991

Forty eight patients diagnosed as atrial septal defect, had been operated from April 1986 to December 1990 at the Department of Thoracic and Cardiovascular Surgery, Inha University Hospital, were analysed retrospectively. Of the 48 patients, 12 patients, were male and 36 patients were female, Their ranged from 8 months to 51 years old, and the mean was 18.0 years old. The proceeding two symptoms were exertional dyspnea and frequent upper respiratory infection. But 10 patients [20.8%] were asymptomatic. Electrocardiographic findings were regular sinus rhythm in 100.0%, RVH in 29.2%, incomplete RBBB in 27.1%, and first degree AV block in 2.1%. Hemodynamic studies were performed in 38 of 48 patients. Significant pulmonary hypertension[over 50mmHg in systolic pulmonary arterial pressure], which is the most serious risk factor, was developed in 4 patients[8.3%]. There was statistical significance between size of defect[cm2/BSA-M2] and systolic pulmonary arterial pressure[sPAP] retrospectively[p=0.036]. We could not find the correlationship between age and Qs % size. Anatomically, secundum type constituted 97.9%, in which 43 patients were PFO type[91.6%] and 3 patients were IVC type[6.3%]. 38 of 48 ASD patients were repaired with patch closure[72.9%] and remains were repaired with direct closure[27.1%]. The operative result was excellent except two cases of transient postoperative arrhythmia. There was no operative mortality.

• The Korean Journal of Emergency Medical Services
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• v.24 no.1
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• pp.67-76
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• 2020

Purpose: Effective time management, as well as life-saving care, are important in maximizing the prognosis of patients who have sustained major traumas. This study evaluated the appropriateness of emergency medical system (EMS) provider's essential care and how this care impacted on-scene time in patients with major traumas. Methods: This retrospective observational study analyzed the EMS major trauma documents, classified according to the physiological criteria (Glasgow coma scale <14, systolic blood pressure <90mmHg, Respiration rate <10 or >29) in Daejeon, from January, 2015 to December, 2018. Results: Of the 707 major trauma cases, the mean on-scene time was 7.75±4.64 minutes. According to EMS guidelines, essential care accuracy was 67.5% for basic airway, 36.4% for advanced airway, 91.2% for cervical collar, 81.5% for supplemental oxygen, 47.0% for positive pressure ventilation, 19.9% for intravenous access and fluid administration, and 96.0% for external hemorrhage control. Factors affecting on-scene time were positive pressure ventilation (p<.004), and intravenous access and fluid administration (p<.002). Conclusion: Adherence to guidelines was low during advanced airway procedures, positive pressure ventilation, intravenous access, and fluid administration. In addition, the on-scene time was prolonged when the practitioner provided positive pressure ventilation, intravenous access, and fluid administration; however, these durations did not exceed the recommended 10 minutes.

• Analytical Science and Technology
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• v.5 no.1
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• pp.51-56
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• 1992

A method to determine the trace level of arsenic in scalp hair by the hydride generation(HG)-inductively coupled plasma(ICP) spectrometry is described. The optimum conditions for the generation of arsine($AsH_3$), and the interference effects from the concomitant ions were studied. Severe interference effect from Ni(II) is circumvented by the coprecipitation of arsenic with $La(OH)_3$. The detection limit of arsenic is 0.3ppm and the arsenic contents in scalp hair ranged 10~20ppb.

• Microbiology and Biotechnology Letters
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• v.19 no.6
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• pp.592-597
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• 1991

An extracellular xylanase of Bacillus stearothemophilus was purified to a single protein through a sequency of operations including ammonium sulfate fractionation, DEAE Sepharose CL-6B ion exchange chromatography, Sephadex G-100 gel filtration and heat treatment. The purified enzyme had a moleular weight of 170, 000. the pH and temperature optima for the enzyme activity were pH 9.0 and $55^{\circ}C$, respectively. The activity was enhanced by $co^{2+} \; and\; Mn^{2+}$, and inhibited by $Hg^{2+}$. Pattern of hydrolysis demonstrated that the xylanase was an endo-splitting enzyme able to break down larchwood xylan at random giving xylobiose and xylotriose as the main end products.

• CELLMED
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• v.5 no.4
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• pp.26.1-26.3
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• 2015

A new physiotherapy named Ciliary Muscle Training method and one successful case had been reported first for treatment of Posner-Schlossman Syndrome (PSS), also known as Glaucomatocyclitic Crisis. When a person observed a back and forth moving object, the ciliary muscle would involuntarily do sometimes tense and sometimes relaxant exercise which can relieve the tensive state of the ciliary muscle of patients suffering PSS disease. Through this period of continued treatment, this ciliary muscle training method can improve the health status of the ciliary muscle and finally achieve the purpose of curing PSS disease. In this successful case, the patient's feedback result was very good after receiving our suggested ciliary muscle training method as an attempt to treat PSS, ill right eye of this PSS patient showed normal appearance without any differences from the other normal eye, his vision test values of naked eye were both 4.9 under nearly 200 degrees of myopia. High intraocular pressure (IOP) was mitigated, measured IOP value was in the normal (≦ 21 mmHg) and comfortable state without PSS relapse until now. Therefore, ciliary muscle training method could be a potential clinical treatment approach to PSS disease.

• Environmental Analysis Health and Toxicology
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• v.19 no.2
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• pp.183-189
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• 2004

To obtain the residual organochlorine pesticides in the culture environment of ginseng on Sangju, Gyeongbuk, the analytical methods for BHC isomers, Aldrin, DDE, Dieldrin, DDD, Endrin and DDT by GCECD are surveyed. The relative retention times for $\alpha$-BHC, $\beta$-BHC, ${\gamma}$-BHC, $\delta$-BHC, Aldrin, DDE, Dieldrin, DDD, Endrin and DDT were 1.000, 1.042, 1.049, 1.086, 1.202, 1.335, 1.341, 1.371, 1.391 and 1.439, respectively The BHC isomers, Aldrin, DDE, Dieldrin, DDD, Endrin and DDT were separated on the base line. The qualified detection concentration for $\alpha$-BHC, $\beta$-BHC, ${\gamma}$-BHC and $\delta$-BHC is 0.43, 1.33, 0.54 and 1.63 ng/g and it's of Aldrin, DDE, Dieldrin, DDD, Endrin and DDT were 0.63, 0.50, 0.66, 0.74, 0.96 and 0.88ng/g, respectively. BHC isomers, Aldrin, DDE, Dieldrin, DDD, Endrin and DDT were not detected in culture environment of ginseng on Sanaju, Gyeongbuk. The detection concentration for Hg, Cd, Pb, Cr, and As in ginseng were 0.0086ppm, 0.0020ppm, 0.0200ppm, 0.0271ppm and 0.0220ppm, respectively, which were also lower than the 30ppm Korea Food & Drug Administration advisory level for heavy metal in herbal medicines.

• Journal of Microbiology and Biotechnology
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• v.8 no.4
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• pp.318-324
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• 1998

A ${\beta}$-galactosidase with high transgalactosylic activity was purified from a Bacillus species, registered as KFCC10855. The enzyme preparation showed a single protein band corresponding to a molecular mass of 150 kDa on SDS-PAGE and gave a single peak with the estimated molecular mass of 250 kDa on Sephacryl S-300 gel filtration, suggesting that the enzyme is a homodimeric protein. The amino acid and sugar analyses revealed that the enzyme is a glycoprotein, containing 19.2 weight percent of sugar moieties, and is much more abundant in hydrophilic amino acid residues than in hydrophobic residues, the mole ratio being about 2:1. The pI and optimum pH were determined to be 5.0 and 6.0, respectively. Having a temperature optimum at $70^{\circ}C$ for the hydrolysis of lactose, the enzyme showed good thermal stability. The activity of the enzyme preparation was markedly increased by the presence of exogenous Mg (II) and was decreased by the addition of EDTA. Among the metal ions examined, the most severely inhibitory effect was seen with Ag (I) and Hg (II). Further, results of protein modification by various chemical reagents implied that 1 cysteine, 1 histidine, and 2 methionine residues occur in certain critical sites of the enzyme, most likely including the active site. Enzyme kinetic parameters, measured for both hydrolysis and transgalactosylation of lactose, indicated that the enzyme has an excellent catalytic efficiency for formation of the transgalactosylic products in reaction mixtures containing high concentrations of the substrate.

• Microbiology and Biotechnology Letters
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• v.15 no.5
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• pp.312-318
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• 1987

The apparent Michaelis constant Km of extracellular adenine deaminase from Streptomyces sp. J-350P was 5.8$\times$10$^{-5}$M. The activation energy or the enzyme was calculated from Arrhenius plots for adenine and the value was 3.13 Kcal/mole. The purine analogues, 6-chloropurine, 2,6-diaminopurine, 6-bromopurine, 4-aminopyrazolo[3,4-d] pyrimidine, 6-iodopurine, and 8-bromoadenine were substrates for the enzyme. 6-Dimethylaminopurine was a competitive inhibitor of the enzyme. The enzyme was inhibited by 0.1mM of Fe$^{3+}$, Ag+, and Hg$^{2+}$ and 1 mM of $\alpha$, $\alpha$＇-dipyridyl, Penta-chiorophenol, and p-chloromercuribenzoate.