• Journal of Microbiology and Biotechnology
• /
• 제15권4호
• /
• pp.683-688
• /
• 2005

A transformation system mediated by Agrobacterium tumefaciens is routinely used for the genetic engineering of plants. Here, we report an efficient and stable method for transformation of heterogeneous genes into an industrial Cephalosporium acremonium by using a similar transformation system established in plants. Both the phleomycin-resistant gene and vgb gene were used as screening markers to confirm the success of transformation by either Southern hybridization or PCR amplification. It was found that acetosyringone (AS) was necessary only for protoplast transformation and the heterogeneous genes transferred were integrated into the genome of C. acremonium. The transformation efficiency obtained with this system was much higher than the conventional techniques used for transformation of C. acremonium.

• Journal of Ginseng Research
• /
• 제48권4호
• /
• pp.366-372
• /
• 2024

Background: The biological activity and pharmacological effects of rare ginsenosides have been proven to be superior to those of the major ginsenosides, but they are rarely found in ginseng. Methods: Ginsenoside Rb1 was chemically transformed with the involvement of methanol molecules by a synthesized heterogeneous catalyst 12-HPW@MeSi, which was obtained by the immobilization of 12-phosphotungstic acid on a mesoporous silica framework. High-performance liquid chromatography coupled with mass spectrometry was used to identify the transformation products. Results: A total of 18 transformation products were obtained and identified. Methanol was found to be involved in the formation of 8 products formed by the addition of methanol molecules to the C-24 (25), C-20 (21) or C-20 (22) double bonds of the aglycone. The transformation pathways of ginsenoside Rb1 involved deglycosylation, addition, elimination, cycloaddition, and epimerization reactions. These pathways could be elucidated in terms of the stability of the generated carbenium ion. In addition, 12-HPW@MeSi was able to maintain a 60.5% conversion rate of Rb1 after 5 cycles. Conclusion: Tandem and high-resolution mass spectrometry analysis allowed rapid and accurate identification of the transformation products through the characteristic fragment ions and neutral loss. Rare ginsenosides with methoxyl groups grafted at the C-25 and C-20 positions were obtained for the first time by chemical transformation using the composite catalyst 12-HPW@MeSi, which also enabled cyclic heterogeneous transformation and facile centrifugal separation of ginsenosides. This work provides an efficient and recyclable strategy for the preparation of rare ginsenosides with the involvement of organic molecules.

• 한국세라믹학회지
• /
• 제34권6호
• /
• pp.553-560
• /
• 1997

A fine $\alpha$-Al2O3 powder was prepared by sol-gel process for membrane application. And it was carried out by adding 1.5wt% $\alpha$-Al2O3 powders(mean size : 87 nm) as seeds to the prepared sols and by controlling the heating schedule (the heating rate and the soaking time) to prevent the microstructural change, which occured during $\theta$-to $\alpha$Al2O3 phase transformation. The seeded $\alpha$-Al2O3 particles acted as the heterogeneous nucleation sites for the $\alpha$-Al2O3 nucleation during the transformation of $\theta$- to $\alpha$-Al2O3 and resulted in increasing the driving force of phase transformation to activate the formation of $\alpha$-Al2O3 phase at 82$0^{\circ}C$. By $\alpha$-Al2O3 seeding and controlling of heating condition the phase transformation of $\theta$- to $\alpha$-Al2O3 was accomplished at low temperature and the grain growth process was depressed. Therefore, the unsupported membrane could be fabricated in $\alpha$-Al2O3 . The average diameter of pores in the fabricated membrane was 7 nm and the porosity was 47%.

• Genomics & Informatics
• /
• 제17권4호
• /
• pp.39.1-39.20
• /
• 2019

Analyzing patterns in data points embedded in linear and non-linear feature spaces is considered as one of the common research problems among different research areas, for example: data mining, machine learning, pattern recognition, and multivariate analysis. In this paper, data points are heterogeneous sets of biosequences (composite data points). A composite data point is a set of ordinary data points (e.g., set of feature vectors). We theoretically extend the derivation of the largest generalized eigenvalue-based distance metric D_ij(γ₁) in any linear and non-linear feature spaces. We prove that D_ij(γ₁) is a metric under any linear and non-linear feature transformation function. We show the sufficiency and efficiency of using the decision rule $\bar{{\delta}}_{{\Xi}i}$(i.e., mean of D_ij(γ₁)) in classification of heterogeneous sets of biosequences compared with the decision rules min_𝚵iand median_𝚵i. We analyze the impact of linear and non-linear transformation functions on classifying/clustering collections of heterogeneous sets of biosequences. The impact of the length of a sequence in a heterogeneous sequence-set generated by simulation on the classification and clustering results in linear and non-linear feature spaces is empirically shown in this paper. We propose a new concept: the limiting dispersion map of the existing clusters in heterogeneous sets of biosequences embedded in linear and nonlinear feature spaces, which is based on the limiting distribution of nucleotide compositions estimated from real data sets. Finally, the empirical conclusions and the scientific evidences are deduced from the experiments to support the theoretical side stated in this paper.

• 산업경영시스템학회지
• /
• 제24권67호
• /
• pp.49-60
• /
• 2001

Data warehouse(DW) manages all information in a Enterprise and also offers the specific information to users. However, it might be difficult to develope an effective DW system due to varieties in computing facilities, data base, and operating systems. The heterogeneous system environments make it harder to extract data and to provide proper information to usesr in real time. Also commonly occurred is data inconsistency of non-integrated legacy system, which requires an effective and efficient data extraction flow control as well as data cleansing. We design the integrated automatic ETT(Extraction Transformation Transportation) system to control data extraction flow and suggest implementation methodology. Detail analysis and design are given to specify the proposed ETT approach with a real implementation.

• 대한원격탐사학회:학술대회논문집
• /
• 대한원격탐사학회 2002년도 Proceedings of International Symposium on Remote Sensing
• /
• pp.885-890
• /
• 2002

As Geographic Information Systems represents three-dimensional topological Information, the systems provide accurate and delicate services for user. In order to execute three-dimensional topological operations, a dimensional transformation and heterogeneous spatial models should be used. However, the existing systems that use the dimensional transformation and the heterogeneous models, are not only difficult to operate the spatial operators, but also happened to support non- interoperability. Therefore, in order to support the spatial operation as well as interoperability between dimensions, we propose three-dimensional spatial operators for the proposed models. We defined the three-dimensional spatial operators prior to designing the proposed model. We also implemented the operators of proposed model and evaluated the implemented model on the component environment. Finally, the proposed model is able to not only support interoperability among systems but also execute spatial queries efficiently on three-dimensional spatial objects.

• 제어로봇시스템학회논문지
• /
• 제17권2호
• /
• pp.102-107
• /
• 2011

In this paper we present cooperation of heterogeneous robot team, composed of a wheeled robot and a helicopter for localization and map building. This heterogeneous robot team can successfully fulfill task by combining the abilities of both robots than single robot because wheeled robot and helicopter have complementing ability. The scenario describes a tightly cooperative task, where the wheeled robot move carrying the helicopter and detect obstacles, if there are obstacles, helicopter take off for map building and land, then robot team move destination avoiding obstacles. We present PID controller for position control of helicopter and transformation algorithm to global coordinate from image pixel coordinate. Experimental result show that the proposed method is valid.

• 한국원자력학회:학술대회논문집
• /
• 한국원자력학회 2005년도 추계학술발표회
• /
• pp.115-116
• /
• 2005

• Earthquakes and Structures
• /
• 제9권2호
• /
• pp.305-320
• /
• 2015

The present paper is devoted to study SH-wave propagation in heterogeneous layer laying over an inhomogeneous isotropic elastic half-space. The dispersion relation for propagation of said waves is derived with Green's function method and Fourier transform. As a special case when the upper layer and lower half-space are homogeneous, our derived equation is in agreement with the general equation of Love wave. Numerically, it is observed that the velocity of SH-wave increases with the increase of inhomogeneity parameter.

• 한국미생물·생명공학회지
• /
• 제23권4호
• /
• pp.425-431
• /
• 1995

A kinetic model of the cyclodextrin formation in a heterogeneous enzyme reaction system using swollen extrusion starch as substrate was derived emphasing the structural features of extrusion starch. The degree of gelatinization, the ratio of accessible and inaccessible portion of extrusion starch, adsorption of CGTase on swollen starch, the structural transformation during reaction, and product inhibition caused by produced CDs were considered in deriving kinetic model. Various kinetic constants were also evaluated. The derived kinetic equation was numerically simulated, which result showed that the derived kinetic equations can be used to predict the experimental data reasonably well under the various experimental conditions. Kinetic model can be utilized for the optimization of enzyme reactor and the process development for CD production from swollen extrusion starch.