• Journal of Genetic Medicine
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• v.4 no.1
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• pp.72-79
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• 2007

Purpose : Glycogen storage disease type III (GSD-III) is a rare autosomal recessive disorder of glycogen metabolism. The affected enzyme, amylo-1,6-glucosidase, 4-alpha-glucanotransferase (AGL, glycogen debranching enzyme), is responsible for the debranching of the glycogen molecule during catabolism. The disease shows clinical and biochemical heterogeneity, reflecting genotype-phenotype heterogeneity among different patients. In this study, we aim at analyzing mutations of the AGL gene in three unrelated Korean GSD-III patients, and characterizing their clinical and laboratory findings. Methods : We characterized the clinical features of three unrelated Korean GSD-III patients by biochemical, histological and imaging studies. The 35 exons and part of exon-intron boundaries of AGL were analyzed by direct sequencing using genomic DNA extracted from the peripheral leukocytes of patients. Results : Diverse clinical features were observed in these patients including hepatomegaly (all patients), seizures (patient 2), grow th failure (patients 1 and 2), hyperlipidemia (patients 1 and 3), raised transaminase and creatine kinase concentrations (all patients), and mild cardiomyopathy (patient 2). Liver transplantation w as performed in patient 2 due to progressive hepatic fibrosis. A dministration of uncooked corn starch maintained normoglycemia and improved biochemical and growth profiles. DNA sequence analysis revealed mutations in 5 out of 6 alleles. Patient 1 was a compound heterozygote of c.1282 G>A (p.R428K) and c.1306delA (p.S603PfsX6), patient 2 had c.1510_1511insT (p.Y 504L fsX 10), and patient 3 had c.3416 T >C (p.L 1139P) and c.1735+1 G>T (p.Y 538_R578delfsX 4) mutations. A part from the p.R428K mutation, the 4 other substitutions identified w ere nov el. Conclusion : GSD-III patients display variable phenotypic characteristics resembling those of GSD-Ia. Molecular defects in the AGL gene of Korean GSD-III patients are genetically heterogeneous.

• Journal of the Korea Society of Computer and Information
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• v.18 no.4
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• pp.131-140
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• 2013

This paper deals with the design and implementation of an interface for interoperation between DiskSim, a well-known disk simulator, and a system-level simulator based on DEVSim++. Such inter-operational simulation aims at evaluation of an overall performance of storage systems which consist of multiple computer nodes with a variety of I/O level specifications. A well-known system-level simulation framework, DEVSim++ environment is based on the DEVS formalism, which provides a sound semantics of modular and hierarchical modeling methodology at the discrete event systems level such as multi-node computer systems. For maintainability we assume that there is no change of the source codes for two heterogeneous simulation engines. Thus, we adopt a notion of simulators interoperation in which there should be a means to synchronize simulation times as well as to exchange messages between simulators. As an interface for such interoperation DiskSimManager is designed and implemented. Various experiments, comparing the results of the standalone DiskSim simulation and the interoperation simulation using the proposed interface of DiskSimManager, proved that DiskSimManager works correctly as an interface for interoperation between DEVSim++ and DiskSim.

• Applied Chemistry for Engineering
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• v.26 no.3
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• pp.336-340
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• 2015

Three different types of polycarbonate (PC)/graphene oxide (GO) composites using diphenyl carbonate as a monomer were fabricated by melt polymerization. Those were the PC/GO composite (PC/GO) using a twin extruder, in-situ PC/GO composite (PC/GO-cat.) using a catalyst, and in-situ PC/GO composite (PC/GO-COCl) using a GO-COCl treated by -COCl, Chemical structures of the composites were confirmed by C-H and C=O stretching peak at $3000cm^{-1}$ and $1750cm^{-1}$, respectively. The slope for the storage (G') versus loss (G") modulus plot decreased with an increase in the heterogeneous property of polymer melts. So we can check the GO dispersion of the PC/GO composites using by the slop for G'-G" plot. According to the G'- G" slopes for three different types of PC/GO composites, GO was well dispersed within PC matrix in case of PC/GO and PC/GO-cat.. It was also confirmed by atomic force microscope (AFM) photos. One of the reasons for the poor GO dispersion of PC/GO-COCl is branching and crosslinking processes occurred during polymerization, which was further confirmed by a plot for the complex modulus versus phase difference.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.11 no.4
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• pp.103-112
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• 1991

In this study, a numerical model was established and applied to simulate the steady-state groundwater and heat flow in an isotropic, heterogeneous, three dimensional aquifer system with uniform thermal properties and no change of state. This model was developed as an aid in screening large groundwater-flow systems as prospects for underground waste storage. Driving forces on the system are external hydrologic conditions of recharge from precipitation and fixed hydraulic head boundaries. Heat flux includes geothermal heat-flow, conduction to the land surface, advection from recharge, and advection to or from fixed-head boundaries. The model uses an iterative procedure that alternately solves the groundwater-flow and heat-flow equations, updating advective flux after solution of the groundwater-flow equation, and updating hydraulic conductivity after solution of the heat-flow equation. Dierect solution is used for each equation. Travel time is determined by particle tracking through the modeled space. Velocities within blocks are linear interpolations of velocities at block faces. Applying this model to the groundwater-flow system located in Jigyung-ri. Songla-myun, Youngil-gun. Kyungsangbuk-do, the groundwater-flow system including distribution of head, temperature and travel time and flow line, is analyzed.

• The KIPS Transactions:PartD
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• v.11D no.1
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• pp.83-94
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• 2004

Recent researches which purport to Integrate distributed information have been concentrated on developing efficient mediator systems that not only provide a high degree of autonomy for local users but also support the flexible integration of required functions for global users. However, there has been little attention on how to evaluate a global query in a mediator. A global query is transformed into a set of its sub-queries and each sub-query is the unit of evaluation in a remote server. Therefore, it is possible to speed up the execution of a global query if the previous results of frequently evaluated sub-queries are materialized in a mediator. Since the Integration schema of a mediator can be incrementally modified and the evaluation frequency of a global query can also be continuously varied, query usage should be carefully monitored to determine the optimized set of materialized sub-queries. Furthermore, as the number of sub-queries increases, the optimization process itself may take too long, so that the optimized set Identified by a long optimization process nay become obsolete due to the recent change of query usage. This paper proposes the adaptive selection of materialized sub-queries such that available storage in a mediator can be highly utilized at any time. In order to differentiate the recent usage of a query from the past, the accumulated usage frequency of a query decays as time goes by.

• Journal of Microbiology and Biotechnology
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• v.19 no.10
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• pp.1085-1091
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• 2009

A phytase with high activity at low temperatures has great potential for feed applications, especially in aquaculture. Therefore, this study used a degenerate PCR and TAIL PCR to clone a phytase gene from Erwinia carotovora var. carotovota, the cause of soft rot of vegetables in the ground or during cold storage. The full-length 2.5-kb fragment included an open reading frame of 1,302 bp and encoded a putative phytase of 45.3 kDa with a 50% amino acid identity to the Klebsiella pneumoniae phytase. The phytase contained the active site RHGXRXP and HD sequence motifs that are typical of histidine acid phosphatases. The enzyme was expressed in Escherichia coli, purified, and displayed the following characteristics: a high catalytic activity at low temperatures (retaining over 24% activity at $5^{\circ}C$) and remarkably thermal lability (losing >96% activity after incubation at $60^{\circ}C$ for 2 min). The optimal phytase activity occurred at pH 5.5 and ${\sim}49^{\circ}C$, and the enzyme activity rapidly decreased above $40^{\circ}C$. When compared with mesophilic counterparts, the phytase not only exhibited a high activity at a low temperature, but also had a low $K_m$ and high $k_{cat}$. These temperature characteristics and kinetic parameters are consistent with low-temperature-active enzymes. To our knowledge, this would appear to be the first report of a low-temperature-active phytase and its heterogeneous expression.

• Journal of Korea Multimedia Society
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• v.3 no.5
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• pp.479-494
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• 2000

Although the proliferation of real-time multimedia services over the Internet might indicate its successfulness in dealing with heterogeneous environments, it is obvious, on the other hand, that the internet now has to cope with a flood of multimedia data which consumes most of network communication channels due to a great deal of video or audio streams. Therefore, for the purpose of an efficient and appropriate utilization of network resources, it requires to develop and deploy a new scalable transmission technique n consideration of respective network environment and individual clients computing power. Also, we can eliminate the waste effects of storage device and data transmission overhead in that the same video stream duplicated according to QoS. The purpose of this paper is to develop a technology that can adjust the amount of data transmitted as an MPEG video stream according to its given communication bandwidth, and technique that can reflect dynamic bandwidth while playing a video stream. For this purpose, we introduce a media scalable media decomposer working on server side, and a scalable media composer working o n a client side, and then propose a scalable transmission method and a media sender and a media receiver in consideration of dynamic QoS. Those methods proposed her can facilitate an effective use of network resources, and provide multimedia MPEG video services in real-time with respect to individual client computing environment.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.6
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• pp.46-54
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• 2018

The Internet of Things (IoT) is an environment in which various devices provide services to users through communications. Because of the nature of the IoT, data are stored and distributed in heterogeneous information systems. In this situation, IoT end applications should be able to access data without having information on where the data are or what the type of storage is. This mechanism is called Service Discovery (SD). However, some problems arise, since the current SD architectures search for data in physical devices. First, turnaround time increases from searching for services based on physical location. Second, there is a need for a data structure to manage devices and services separately. These increase the administrator's service configuration complexity. As a result, the device-oriented SD structure is not suitable to the IoT. Therefore, we propose an SD structure called Name-based Service-centric Service Discovery (NSSD). NSSD provides name-based centralized SD and uses the IoT edge gateway as a cache server to speed up service discovery. Simulation results show that NSSD provides about twice the improvement in average turnaround time, compared to existing domain name system and distributed hash table SD architectures.

• The KIPS Transactions:PartA
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• v.15A no.1
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• pp.53-60
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• 2008

Streaming media has contributed to a significant amount of today's Internet Traffic. Like traditional web objects, rich media objects can benefit from proxy caching, but caching streaming media is more of challenging than caching simple web objects, because the streaming media have features such as huge size and high bandwidth. And to support various bandwidth requirements for the heterogeneous ubiquitous devices, a transcoding proxy is usually necessary to provide not only adapting multimedia streams to the client by transcoding, but also caching them for later use. The traditional proxy considers only a single version of the objects, whether they are to be cached or not. However the transcoding proxy has to evaluate the aggregate effect from caching multiple versions of the same object to determine an optimal set of cache objects. And recent researches about multimedia caching frequently store initial parts of videos on the proxy to reduce playback latency and archive better performance. Also lots of researches manage the contents with segments for efficient storage management. In this paper, we define the 9-events of transcoding proxy using 4-atomic events. According to these events, the transcoding proxy can define the next actions. Then, we also propose the segment-based caching policy for the transcoding proxy system. The performance results show that the proposing policy have a low delayed start time, high byte-hit ratio and less transcoding data.

• Journal of KIISE:Computing Practices and Letters
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• v.16 no.1
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• pp.1-10
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• 2010

Recently heterogeneous storage system such as hybrid hard disk drive (H-HDD) combining flash memory and magnetic disk is launched, according as the read performance of NAND flash memory is enhanced as similar to that of hard disk drive (HDD) and the power consumption of NAND flash memory is reduced less than that of HDD. However, the read and write operations of NAND flash memory are slower than those of rotational disk. Besides, serious overheads are incurred on CPU and main memory in the case that intensive write requests to flash memory are repeatedly occurred. In this paper, we propose the Least Frequently Used-Hot scheme that replaces the data blocks whose reference frequency of read operation is low and update frequency of write operation is high, and the data flushing scheme that rearranges the data blocks into the multi-zone of the rotation disk. Experimental results show that the execution time of the proposed method is 38% faster than those of conventional LRU and LFU block replacement schemes in I/O performance aspect and the proposed method increases the life span of Non-Volatile Cache 40% higher than those of conventional LRU, LFU, FIFO block replacement schemes.