• Journal of Applied Biological Chemistry
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• v.62 no.3
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• pp.265-273
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• 2019

This study aimed to evaluate the anti-atherosclerotic and anti-hypertensive effects of six different plant extracts using a N(G)-nitro-L-arginine-methyl ester (L-NAME)-induced rat model of hypertension. All extracts were administered orally for six weeks. At the end of the study period blood pressure, blood flow, aortic histopathology, and hepatic endothelial nitric oxide synthase (eNOS) expression were measured. Subsequently, we also measured the levels of intracellular reactive oxygen species, nitric oxide (NO), and anti-inflammatory cytokines in vitro. Based on these screening results, we selected extracts of Cinnamomum cassia (C. cassia) and Salvia miltiorrhiza (S. miltiorrhiza) for further evaluation. C. cassia and S. miltiorrhiza extracts ameliorated hypertension and atherosclerosis in L-NAME-treated rats in a dose-dependent manner. In addition, a mixture of C. cassia and S. miltiorrhiza had an additive effect to reduce blood pressure, increase blood flow, and normalize aortic tissue. This mixture demonstrated anti-oxidative and anti-inflammatory activities in vitro. In conclusion, although further analysis of the therapeutic mechanism is required, the anti-hypertensive and anti-atherosclerotic effects of this mixture are likely mediated by increased eNOS expression, and its anti-oxidative and anti-inflammatory activities.

• Research in Plant Disease
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• v.24 no.1
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• pp.52-58
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• 2018

Pear scab and rust are the major diseases causing severe epidemics in organic cultivation of pear trees. Incidences of pear scab and rust were compared in organically managed plots and conventionally managed plots to obtain optimum application schedule of environment-friendly control agents in organically managed plots. Organically cultural practice with 10 time-applications of lime sulfur and Bordeaux mixture showed higher than 40% of control efficacies of pear scab and rust compared to conventionally cultural practice. Organically cultural practice with 8 time-applications of lime sulfur considering weather condition showed higher than 30% of control efficacies of pear scab compared to conventionally cultural practice. The results suggest that proper application of environment-friendly control agents such as lime sulfur considering weather condition will enable effective control of the major diseases for organic cultivation of pear.

• Korean Journal of Medicinal Crop Science
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• v.22 no.3
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• pp.231-236
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• 2014

This experiment was carried out to establish the optimum cutting time, plant growth regulator, and bed-soil for rooting by greenwood cutting of Schisandra chinensis. Approximately 7 cm long-shoots of greenwood cuttings were transplanted by various cutting times, plant growth regulators, and bed-soils in the plastic-film house. The rooting rate of greenwood cutting was 12.5% in the April 5th, 73.5% in the May 8th, and 75.5% in the August 5th. The number and length of primary root in greenwood cutting were more in the early May than those in the early August. For mass propagation of Schisandra chinensis. using greenwood cutting, shoots were treated with plant growth regulators on May to increase rooting rate. Rooting rate was 100% with IAA $50mg/{\ell}$, 92.9% with NAA $100mg/{\ell}$, and NAA $1,000mg/{\ell}$, for 60 min. To select effective media for rooting, various medias for bed-soil were treated by single and mixture form for 100 days after cutting. Rooting rate was 91.8% in the single treatment of peat moss or decomposition of granite soil, and this result was better than those in other treatment. The treatment by 1 : 1 mixture of peat moss and horticulture bed soil was rooting with 94.0% best rooting rate.

• Korean Journal of Plant Resources
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• v.23 no.4
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• pp.379-385
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• 2010

This study was carried out to develop cultivation techniques of Climacium japonicum in transparent pot. We analyzed effect of different soil moisture content, air exposure degree, bed soil and ground cover moss on the growth of Climacium japonicum. The best humidity condition in transparent pot culture was 60~80% and a mixture of bed soil with peatmoss : perlite as 7:3 ratio was effective to growth. Two holes(each diameter is 4 mm) in the cover were also effective to the growth and control moisture in the transparent pot. The best mixture of bed soil was moss with peatmoss : pelite(7:3) in the pot culture but price of moss is expensive, the moss treatment was not practical. Total of 10 moss species were selected as ground cover plant; Hypnum erectiusculum Sull. et Lesq., Thuidium kanedae Sak., Hypnum plumaeforme Wils., Trachycystis microphylla Lindb., Bryum argenteum Hedw., Hypnum oldhamii Jaeg., Funaria hygrometrica Hedw., Leucobryum glaucum Aongstr., Polytrichum commune Hedw. and Weissia controversa Hedw. The plant height of selected moss was smaller than that of Climacium japonicum so these moss were very effective to cover the ground.

• Journal of Bio-Environment Control
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• v.19 no.4
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• pp.311-316
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• 2010

This study was conducted to investigate the effect of anaerobic fermentation of wheat bran to prevent root-knot nematodes which are infected in plastic house due to over 10 years continuous cultivation of fruits and vegetables. Anaerobic fermentation treatment of wheat bran was done for 20 days by mixture of 2,000 kg fresh wheat bran per 10 are and soil with water in 30 cm soil depth. Chemical treatment of fosthiazate was done by mixture of 6 kg soil for 7 days. Both treatments show suppression of density of rootknot nematodes, especially in anaerobic fermentation treatment. Anaerobic fermentation treatment keeps the low level of root-knot nematode density until 90 days of cultivation and also showed good effect of melon growth. Related with death percentage of melon plant, anaerobic fermentation treatment shows only 3% and also large size of quality fruit but control 65%. Anaerobic fermentation treatment of wheat bran have proved to control the level of root-knot nematodes instead of synthetic chemicals for at least one cropping season and it showed good effect to fruit quality.

• Korean Journal of Medicinal Crop Science
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• v.21 no.1
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• pp.7-19
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• 2013

This study was carried out to select new pelleting binder and material for seeds from Fraxinus rhynchophylla Hance and Alnus sibirica Fisch. ex Turcz. The optimum treatments of the various concentrations and species of priming agents to improve seed germination of both woody medicinal plants were also estimated. Germinability was increased when the seeds of Fraxinus rhynchophylla Hance was soaked in -1.0 MPa of PEG6000 solution at $15^{\circ}C$ for 4 days significantly, the optimum treatment for improving germination of Alnus sibirica Fisch. ex Turcz was observed when the tested seeds was soaked in 100 mM of KCl at $15^{\circ}C$ for 4 days. The influence of physical and chemical properties of pelleting solid materials, the mixture of gypsum, diatomaceous earth, dalma ceramic and vermicuolite (6:1:1:1 ratio) were found as the best pelleting materials for Fraxinus rhynchophylla Hance and Alnus sibirica Fisch. ex Turcz. seeds. To satisfy the requirements of absorption and compatibility for multi-layer seed pelleting, SGPA (Starch-grafted cross-linked polyacrylates) hydrogel was prepared using starch, acrylonitrile, ceric ammonium nitrate, nitric acid, methyl alcohol and potassium hydroxide. The resulting SGPA hydrogel showed high water absorption but not plant compatibility. It suggested that seed pelleting using pelleting materials and SGPA hydrogel (multi-layer coating) after priming agent treatment is to increase germinability and seedling growth and it can reduced irrigation labours and can also save seed.

• Korean Journal of Medicinal Crop Science
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• v.22 no.4
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• pp.270-275
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• 2014

This study was conducted to select and develop effect of eco-friendly organic materials for the eco-friendly prevention of Anthracnose occurred in the ginseng. Anthracnose on ginseng is occurred by Colletotrichum gloeosporioides and the crop damage is severe especially from July to August after rainy season. The test results showed that control effect by test products materials on the three years ginseng and four years ginseng field was lower in eco-friendly organic materials than that of chemical pesticide. However, the control effect of bordeaux mixture was higher with 71.3% and 73.8% levels than those of mineral matter, microbial agent, and developed plants extract mixtures (Eugenol, Curcumin, Wood vinegar, etc). On the other hand, three types of developed plants extract mixtures (3 types) showed control effect in a range of from 58.1% to 63.6% against Anthracnose which was higher as compared with plant extract alone and sodium silicate regardless of ages of ginseng. The results of this study would attribute in verifying the control effect of eco-friendly materials against Anthracnose for ginseng through investigating antimicrobial compounds contained in the plants body. Also, it would be used as control method against Anthracnose occurred in ginseng by judging the right control time through monitoring occurrence of disease.

• Mass Spectrometry Letters
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• v.14 no.2
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• pp.25-35
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• 2023

Plants are a traditional source of many chemicals used as biochemical, flavors, food, color, and pharmaceuticals in various countries, especially India. Most herbal medicines and their derivatives are often made from crude extracts containing a complex mixture of various phytochemical chemical components (secondary metabolites of the plants). This study aimed to identify bioactive compounds from the different parts of the plant from the ethanolic extract of Gymnema sylvestre, Senna auriculata, and Cissus quadrangularis (leaves, flower, stem) by gas chromatography-mass spectroscopy (GC-MS). The gas chromatography - mass spectrometry analysis revealed the presence of various compounds like 3,4-dimethylcyclohexanol, hexanoic acid, D-mannose, and N-decanoic acid. Hence, the Gymnema sylvestre, Senna auriculata, and Cissus quadrangularis may have chemopreventive, anti-cancer, anti-microbial activity, antioxidant, anti-diabetic activity, anti-inflammatory, and antifungal due to the presence of secondary metabolites in the ethanolic extract. These phytochemicals are supported for traditional use in a variety of diseases.

• 보존과학연구
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• s.33
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• pp.5-17
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• 2012

An Ola leaf manuscript, which consists of words carved on an Ola leaf and is filled up with a mixture of herbal oils made from Vateria acuminata Hyne and charcoal, is one of the native writing media in Sri Lanka. According to historical records, Ola leaf manuscripts had been used from the 1st to the 18th century A.D. From the recent findings that Ola leaf manuscripts have been preserved well for the past 400 years and are in good state of preservation, it is supposed that herbal oils preserve Ola leaves against environmental and biological factors such as fungi and insects. To evaluate the anti-biological susceptibility of the herbal oils, the molds isolated from wooden printing blocks in Janggyeong Panjeon of Haeinsa Temple in South Korea and bacteria and fungi isolated from Ola leaves were cultured. After spreading the microorganisms suspension on an agar plate, a disk paper containing a certain volume of herbal oil was placed on the agar plate. It was found in the experiment that herbal oil exhibits a clear zone, which is optically clear and inhibits the growth of microorganisms, against some molds and bacteria. The study results indicate that the herbal oil from the plant Vateria acuminata Hyne has the anti-bacterial and anti-fungal properties.

• Korean Journal of Plant Resources
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• v.21 no.1
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• pp.5-11
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• 2008

This study examined the characteristics of Pinus thunbergii community that forms a windbreak forest around the coast of Gwanmaedo located in Jodo-myeon, Jindo-gun, Jeollanamdo which is located in the south-western areas of Korea from December 2005 to April 2007 and to suggeste the ecological management methods of coastal windbreak areas. P. thunbergii community, a coastal windbreak forest of Gwanmaedo, was classified into disturbance, growth, mixture, stability, and back mountain vegetation according to major companions species and vegetation types. P. thunbergii community of disturbance and growth vegetation needs active management through tree thinning, mowing, weeding out, use of rest space, and felling sick pine trees. P. thunbergii community of mixture, stability, and back mountain vegetation needs active preservation of a coastal windbreak to restore natural vegetation by making a windbreak walk and a forest buffer zone and inducing vegetation succession. Accordingly, in this study, ecological management methods were suggested according to the actual state of distribution by habitat characteristics of coastal windbreak areas such as management of beaches and surrounding area of idle lands, restoration of back wetlands, inhibition of foreign plants, maintenance of diversity of species and habitats, and prevention of aging and spread of damage from insects.