• Title/Summary/Keyword: Herbal complex

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Effects of Herbal Medicine Complex on Skin Inflammation and Atopic Dermatitis (한방 복합물이 피부 염증 및 아토피 피부염에 미치는 영향)

  • Ji-Hee, Choi;In-Hwan, Joo;Jong-Min, Park;Dong-Hee, Kim
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.36 no.5
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    • pp.187-192
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    • 2022
  • The purpose of this study is to examine the effect of herbal medicine complex (HMC) containing Camellia sinensis L., Duchoesna chrysantha, Houttuynia cordata Thunberg, Poncirus trifoliata Rafinesque on skin inflammation and atopic dermatitis. First, we examined the anti-inflammatory effect of HMC in TNF-α induced human keratinocytes (HaCaT cell). Real-time PCR and western blotting were performed to evaluate the expression of inflammatory cytokines (e.g., iNOS, COX-2, IL-6, IL-8) mRNA and protein. Four-weeks old male NC/Nga mice were treated with 1% 2,4-dinitrochlorobenzene (DNCB) solution and used as an atopic dermatitis mice model. And, HMC (200 mg/kg or 400 mg/kg) was administered directly into the stomach of mice for 4 weeks, and blood or serum analysis, tissue staining were performed after oral gavage. As a result HMC inhibited the mRNA expression of iNOS, COX-2, IL-6, and IL-8, which had been increased by TNF-α in HaCaT cells. In addition, the protein expression was also significantly suppressed in the same way as the mRNA expression results. The in vivo experiment results showed that, HMC administration reduced thickening of the epidermis and infiltration of eosinophil into the skin stratum basale compared to DNCB treatment. In addition, HMC administration significantly reduced the inflammatory cytokines (IL-4, IL-5, IL-6, and IL-13) production and immunocyte (white blood cell, lymphocyte, neutrophil, and eosinophil) count compared to DNCB treatment. Moreover, the serum IgE and histamine level was decreased by HMC administration. These results suggest that HMC can be used as effective herbal medicine extract for skin inflammation and atopic dermatitis. And this study may contribute to the development of the herbal medicine-based drug for the treatment of skin inflammation and atopic dermatitis.

Development of a Segregating Population with Biological Efficiency in Agaricus bisporus

  • Oh, Youn-Lee;Sonnenberg, Anton S.M.;Baars, Johan J.P.;Jang, Kab-Yeul;Oh, Min ji;Im, Ji-Hoon;Kong, Won-Sik
    • The Korean Journal of Mycology
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    • v.45 no.4
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    • pp.328-335
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    • 2017
  • In this study, we made a population with high biological efficiency (BE) to investigate the complex genetic architecture of yield-related traits in Agaricus bisporus. MB-013 crossed between bisp 015-p2 and bisp 034-p2, had high BE. Additionally MB-013 was an intervarietal hybrid that intercrosses with A. bisporus var. burnettii, bisp 015, and A. bisporus var. bisporus, bisp 034. One hundred and seventy homokaryons were selected using the cleaved amplified polymorphic sequence (CAPS) markers (PIN primer/HaeIII) from 300 single spore isolates (SSIs). One hundred $BC_1F_1$ hybrids were obtained by crossing the homokaryons of MB-013 with bisp15-p1. The population of 100 BC1F1 hybrids is suitable for analyses of BE.

Constituents and Effects of Ginseng Leaf

  • Xie, Jing-Tian;Wu, Ji-An;Lin, Elaine;Wang, Chong-Zhi;Yuan, Chun-Su
    • Advances in Traditional Medicine
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    • v.4 no.1
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    • pp.1-8
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    • 2004
  • Ginseng root has been used as a tonic remedy in Traditional Chinese Medicine for centuries. Modern studies have demonstrated that ginseng root has complex components and multiple pharmacological properties. The effects of ginseng leaf, however, are not well known. Recent studies show that compared to ginseng root, ginseng leaf and stem exhibit a higher content of active compositions such as ginsenosides, polysaccharides, triterpene flavonoids, volatile oil, polyacetylenic alcohols, peptides, amino acids and fatty acids. Ginseng leaf possesses multiple pharmacological effects in the central nervous, cardiovascular, growth and metabolism systems. Additionally, the leaf has anti-fatigue, anti-hyperglycemic, anti-oxidant, and anti-aged effects. In general, ginseng leaf is quite safe, but adverse effects may occur if it is abused or is of poor quality. Thus, attention must be paid to dosages, quality, and standardization of ginseng leaf products.

Trends in Animal Experiments for Herbal Medicine Treatment of Atopic Dermatitis: During the 10 year's results (한약의 아토피피부염에 대한 국내외 동물실험 연구 동향: 최근 10년을 중심으로)

  • Jeong Aram
    • The Journal of Pediatrics of Korean Medicine
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    • v.37 no.3
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    • pp.49-74
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    • 2023
  • Objectives The aim of this study was to analyze the research trends in herbal medicine as a treatment for atopic dermatitis (AD) in an animal model. Methods We conducted a search targeting papers published from 2013 onwards in Korean and English databases. The search terms were focused on "AD", "dermatitis", and "Korean medicine". Only animal experimental studies involving the oral administration of single or complex prescriptions of Korean medicine were included, whereas in vitro studies, local administration studies, and studies of specific components of Korean medicine were excluded. We searched the PubMed and Embase databases for English databases and the Science on Research Information Sharing Service (RISS) and Korean Studies Information Service System (KISS) databases for Korean studies. Results Among the herbal medicine intervention groups, the most commonly used single herbs were baekseonpi, danggui, and douchi, whereas a mixture of hwangryun and gamcho was used in three studies, making it the most frequently used combination. There were significant improvements in indicators related to the symptoms of AD and various pro-inflammatory markers. Conclusions These results indicate the efficacy of herbal medicines in the treatment of AD in animal models. However, this study had several limitations. Therefore, future systematic reviews that address these limitations and provide more comprehensive analyses are required.

The Effect of Oral Administration of Herbal Medicines on Memory in Alzheimer's Disease Animal Models: A Review of Animal Study Reports Published in Korea (알츠하이머병 유발 동물모델에서 한약제재 경구투여가 기억에 미치는 영향에 대한 국내 연구보고 고찰)

  • Han, Da-Young;Park, Na-Eun;Kim, Sang-Ho;Chung, Dae-kyoo
    • Journal of Oriental Neuropsychiatry
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    • v.28 no.4
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    • pp.359-371
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    • 2017
  • Objectives: The objective of this study was to review the effect of oral administration of herbal medicines on the improvement of memory in Alzheimer's disease animal model reported in Korean domestic journals. Methods: The Korean databases (Koreantk, KISS) were searched with memory as a popular search term. During the searches, only animal study reports were reviewed. Data of animal models, intervention, observation methods of measuring indicators were extracted from the databases. Results: Typically, 36 articles were reviewed. Twenty-two studies used scopolamine to induce Alzheimer's disease, 24 studies used complex herbal medicines, and 12 studies used simple herbal medicines. Polygalae Radix and Acori Rhizoma were the most frequently used herbal medicines to improve memory in Alzheimer model. To evaluate the effect of herbal medicines, 36 studies used macroscopy, 16 studies used molecular biological analysis, 21 studies used biochemical analysis, 15 studies used histological analysis, and 11 studies used hematological analysis. Each study showed significant improvement with respect to memory indicators. Conclusions: Overall, the results suggest that treatment employing herbal medicines is an effective option to treat memory impairment in Alzheimer's disease.

A Review of Etiology, Syndrome Differentiation, and Herbal Medicine of Epilepsy (뇌전증의 병인, 변증, 한약 치료에 대한 고찰 -한국, 일본, 중국 데이터베이스를 중심으로-)

  • Kim Hye Yeon;Kim Tae Hwan;Han Ju Hui;Bang Mi Ran;Chang Gyu Tae;Lee Jin Yong;Kim Hyo In;Lee Donghun;Lee Sun Haeng
    • The Journal of Pediatrics of Korean Medicine
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    • v.38 no.3
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    • pp.66-96
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    • 2024
  • Objectives This study aimed to establish a foundation for research on Korean medicine for epilepsy by analyzing its etiology, syndrome differentiation, and the use of herbal medicines. Methods Articles were extracted from five databases in Korea, Japan, and China: the Oriental Medicine Advanced Searching Integrated System (OASIS), Citation Information by the National Institute of Informatics (CiNii), Chinese National Knowledge Infrastructure (CNKI), Wangfang Database, and Chinese Scientific Journal Database (VIP). The etiology, syndrome differentiation, and herbal medicines used for epilepsy were investigated. Results A total of 64 articles were selected, including seven clinical studies, 16 case reports, and 41 reviews. Epilepsy is a complex disease with various etiologies, and among the five viscera, syndrome differentiation mainly involved the liver, spleen, and kidney, with pathological factors that included phlegm, wind, and blood stasis. Frequently used herbal medicines included Modified Jeongganhwan, Sihogyejitang, Yukgunjatang, and Sihogayonggolmoryotang. Conclusions This study analyzed epilepsy's etiology, syndrome differentiation, and herbal treatments. Further evidence is needed to better understand the efficacy and safety of Korean herbal medicine for treating epilepsy.

A Case Report of Ge-yan-jiu & Herbal Medicine Complex Treatment on Jue zheng (厥證) caused by Wang yang(亡陽) (격염구(隔鹽灸)와 사역탕(四逆湯)으로 치료한 상한후(傷寒後) 궐증(厥證) 환자에 대한 증례보고)

  • Choi, You-Kyung;Kang, Mi-Suk
    • The Journal of Internal Korean Medicine
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    • v.28 no.1
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    • pp.187-192
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    • 2007
  • Purpose : The purpose of this case is to report the improvement after acupuncture & moxibustion complex therapy about Jue zheng (厥證) caused by Shang han(傷寒). Methods : We provided acupuncture & moxibustion complex therapy to a patient who suffered from chills and coldness. We used acupuncture technique and Ge-yan-jin in umbilicus (神厥 隔鹽灸). Results & Conclusions : We observed that acupuncture & moxibustion complex therapy decreased symptoms of Jue zheng (厥證) and improved general condition of a patient who suffered from chills and coldness.

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Effects of Temperature and UV Irradiation on Stability of Anthocyanin-Polyphenol Copigment Complex in Mulberry Fruits (오디에서 열처리와 자외선 조사가 Anthocyanin-Polyphenol Copigment Complex의 안전성에 미치는 효과)

  • Bang, In-Soo;Yu, Chang-Yeon;Lim, Jung-Dae
    • Korean Journal of Medicinal Crop Science
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    • v.18 no.3
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    • pp.191-200
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    • 2010
  • Anthocyanin and polyphenolic compounds present in fruits of mulberry (Morus alba L.) were determined and the influence of temperature and UV irradiation on stability of the anthocyanin-copigment complex were investigated. The copigmentation substance selected in non-anthocyanin fraction from mulberry for the study included: phenolic acid (hydroxybenzoic acid) and flavonoid (quercetin-3-O-$\beta$-D-glucopyranoside). The copigmentation effect increased with the copigment content. UV irradiation had a stronger degradation effect on the copigmentation complex than heating at $80^{\circ}C$. The non-anthocyanin fraction of mulberry and isolated flavonoid (quercetin-3-O-$\beta$-D-glucopyranoside) from mulberry fruit predominated over other copigment substances.

Compilation of 104 Experimental Theses on the Antitumor and Immuno-activating therapies of Oriental Medicine (한의학의 항종양 면역치료에 관한 연구 -1990년 이후 발표된 실험논문을 중심으로-)

  • Kang Yeon Yee;Kim Tai Im;Park Jong Ho;Kim Sung Hoon;Park Jong Dai;Kim Dong Hee
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.17 no.1
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    • pp.1-24
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    • 2003
  • This study was done to compile 104 experimental theses which are related to the antitumor and immuno-activating therapies between February 1990 through February 2002. Master's and doctoral theses were dassified by schools, degrees, materials, effects, experimental methods of antitumor and immunoactivity, and results. The following results were obtained from this study : 1. Classifying the theses by the school, 34.6% were presented by Daejeon University, 29.8% by Kyung-hee University and 11.5% by Won-kwang University. Of all theses, 51.0% were aimed for the doctoral degree and 43.3% were for the master's degree. All of three universities have their own cancer centers. 2. Classifying the theses by herb materials, complex prescription accounted for 60.3%, single herb accounted for 24.8% and herbal acupuncture accounted for 14.2%. Considering the key principles of the traditional medicine, complex prescription was much more thoroughly studied than single herb prescription. The results showed that the complex prescription had both antitumor activity and immuno-activating activity, which might reflects on multi-activation mechanisms by complex components. 3. Classifying the theses by the efficacy of herbs examined, in single herb, invigorating spleen and supplementing was 35.5%, expelling toxin and cooling was 29.0%, activating blood flow and removing blood stasis was 12.9%. In herbal acupuncture, invigorating spleen and supplementing was 52.9%, expelling toxin and cooling was 29.4%. In complex prescription, pathogen-free status was 41.9%, strengthening healthy qi to eliminate pathogen was 35.5%, strengthening healthy qi was 22.6%. It is presumed that the antitumor and immunoactivating therapy based on syndrome differentiation is the best way to develop oriental oncology. 4. Classifying the theses by antitumor experiments, cytotoxic effect was 48.1 %, survival time was 48.1 % and change of tumor size was 42.3%. Survival rate was not necessarily correlated with cytotoxicity. These data reflect the characteristic, wholistic nature of the oriental medicine which is based on BRM (biological response modifier). 5. Classifying the theses by immunoactivating experiments, hemolysin titer was 51.0%, hemagglutinin titer was 46.2% and NK cell's activity was 44.2%. In the future studies, an effort to elucidate specific molecular and cellular mechanisms of cytokine production in the body would be crucial. 6. Classifying the theses according to the data in terms of antitumor activity, 50% was evaluated good, 24.0% was excellent, and 15.5% have no effect. In an evaluation of immuno-activating activity, 35.9% was excellent and 18.0% showed a little effect. The index point, as described here, may helps to use experimental data for clinical trials. Changes in index points by varying dosage implicate the importance of oriental medical theory for prescription. 7. In 167 materials, IIP (immunoactivating index point, mean : 3.12±0.07) was significantly higher than AIP(antitumor index point, mean : 2.83±0.07). These data demonstrate that the effect of herb medicine on tumor activity depends more on immunoactivating activity than antitumor activity. This further implies that the development of herbal antitumor drugs must be preceded by the mechanistic understanding of immunoactivating effect. 8. After medline-searching tumor and herb-related articles from NCBI web site, we conclude that most of the studies are primarily focused on biomolecular mechanisms and/or pathways. Henceforth, we need to define the biomolecular mechanisms and/or pathways affected by herbs or complicated prescriptions. 9. Therefore, the most important point of oriental medical oncology is to conned between experimental results and clinical trials. For the public application of herbal therapy to cancer, it is critical to present the data to mass media. 10. To develop the relationship of experimental results and clinical trials, university's cancer clinic must have a long-range plan related to the university laboratories and, at the same time, a regular consortium for this relationship is imperative. 11. After all these efforts, a new type herbal medicine for cancer therapy which is to take care of the long-term administering and safety problem must be developed. Then, it would be expected that anti-tumor herbal acupuncture can improve clinical symptoms and quality of life (QOL) for cancer patients. 12. Finally, oriental medical cancer center must be constructed in NCC (National Cancer Center) or government agency for the development of oriental medical oncology which has international competitive power.

A Case Report of Lower Extremity Paresthesia with Polyneuropathy Treated with Traditional Korean Complex Treatment (양하지 이상감각을 호소하는 다발신경병증 환자에 대한 침구-한약 복합한방 치료 치험 1례)

  • Lee, Hyun-ku;Kim, Se-won;Seo, Yu-na;Bae, In-hu;Park, Ho-jung;Cho, Ki-ho;Moon, Sang-kwan;Jung, Woo-sang;Kwon, Seung-won
    • The Journal of Internal Korean Medicine
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    • v.41 no.2
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    • pp.256-266
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    • 2020
  • Objectives: This report presents the case of a 21-year-old woman with lower extremity paresthesia in both feet due to polyneuropathy. Methods: The patient was treated using a Korean traditional complex treatment approach that included herbal medication, acupuncture, electroacupuncture, and moxibustion. We evaluated the improvements in the pain symptoms in both feet using the numeric rating scale (NRS); we also assessed for gait disturbance and used Digital Infrared Thermographic Imaging (D.I.T.I.) to evaluate minute changes in body temperature in diseased areas. Results: After administering the Korean traditional complex treatment, we observed a decrease in pain levels in both feet based on the NRS scores. We also observed improvements in gait disturbance and D.I.T.I. Conclusions: This case showed that the use of a Korean traditional complex treatment approach, consisting of herbal medication, acupuncture, electroacupuncture, moxibustion, had a positive effect on decreasing polyneuropathy symptoms.