• Archives of Pharmacal Research
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• v.28 no.3
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• pp.269-273
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• 2005

A furocoumarin derivative, psoralen (7H-furo[3,2-g][1]benzopyran-7-one), was isolated from the n-hexane fraction of Heracleum moellendorffii Hance. We examined the effects of psor-alen on a human Kv1.5 potassium channel (hKv1.5) cloned from human heart and stably expressed in Uk- cells. We found that psoralen inhibited the hKv1.5 current in a concentration-, use- and voltage-dependent manner with an IC$_{50}$ value of 180 $\pm$ 21 nM at +60 mV. Psoralen accelerated the inactivation kinetics of the hKv1.5 channel, and it slowed the deactivation kinetics of the hKv1.5 current resulting in a tail crossover phenomenon. These results indicate that psoralen acts on the hKv1.5 channel as an open channel blocker. Furthermore, psoralen prolonged the action potential duration of rat atrial muscles in a dose-dependent manner. Taken together, the present results strongly suggest that psoralen may be an ideal antiarrhythmic drug for atrial fibrillation.

• Food Science and Preservation
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• v.16 no.5
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• pp.765-771
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• 2009

Anti-oxidative activities of Heracleum moellendorffii Hance extracts were measured after extraction with 50% (v/v) ethanol or water (at $80^{\circ}C$ or $20^{\circ}C$). The total polyphenol content was highest (64.73 mg GAE/g) in extract from 50% ethanol extraction at $80^{\circ}C$. In extracts obtained by water extraction at $80^{\circ}C$ and $20^{\circ}C$, the total polyphenol contents were 39.78 mg GAE/g and 23.17 mg GAE/g, respectively. The antioxidant activities of the ethanol extract at $80^{\circ}C$ were highest, as assessed by DPPH radical scavenging activity (69.14% at 50 ppm), reducing power (OD 0.93), and $ABTS^+$ radical-scavenging activity (85.62% at 1,000 ppm). At $80^{\circ}C$, the antioxidant activity of the 50% (v/v) ethanol extract was 8-20% higher than that of the water extract. Also, the chemical composition of extract from ethanol extraction at $80^{\circ}C$ was analyzed. The levels of moisture, crude protein, crude fat, and crude ash were 6.38%, 4.35%, 0.67%, and 1.96%, (all w/w), respectively. The Na, K, Mg, Zn, Fe, Ca, and P contents of the extract were 53.41, 398.26, 5.80, 0.40, 5.27, 3.56, and 47.17 mg/100 g, respectively. The total levels of polyphenolic compounds and flavonoids in the ethanol extract were 64.73 mg GAE/g and 49.54 mg RE/g.

• The Korea Journal of Herbology
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• v.31 no.5
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• pp.15-23
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• 2016

Objectives : Araliae Continentalis Radix and Angelicae Pubescentis Radix have been used as the same medicinal name Korean and Chinese traditional medicines, respectively. The authentic Araliae Continentalis Radix is described only the root of Aralia continentalis in the Korean Pharmarcopoeia. However, the dried root of Angelica biserrata, Levisticum officinale, or Heracleum moellendorffii also has been distributed adulterants of Araliae Continentalis Radix. To develop a reliable method for identifying Araliae Continentalis Radix from adulterants, we carried out the analyses of universal DNA barcode sequences.Methods : Four plants species were collected from different habitate and nucleotide sequences of matK and rbcL were analyzed. The species-specific sequences and phylogenetic relationship were estimated using entire sequences of two DNA barcodes, respectively.Results : In comparative analysis of matK sequences, we were identified 104 positions of marker nucleotide for Ar. continentalis, 3 for An. biserrata, 4 for L. officinale and 8 for H. moellendorffii enough to distinguish individual species, respectively. Furthermore, we obtained marker nucleotides in rbcL at 42 positions for Ar. continentalis, 5 for An. biserrata and 2 for H. moellendorffii, but not for L. officinale. The phylogenetic tree of matK and rbcL were showed that all samples were clustered into four groups constituting homogeneous clades within the species.Conclusions : We confirmed that species-specific marker nucleotides of matK sequence provides distinct genetic information enough to identify four species. Therefore, we suggest that matK gene is useful DNA barcode for discriminating authentic Araliae Continentalis Radix from inauthentic adulterants.

• Korean Journal of Medicinal Crop Science
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• v.18 no.5
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• pp.329-337
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• 2010

'Angelicae Pubescentis Radix' (APR) is an important oriental medical preparation. In Korea, Aralia continentalis has been recognized as the source plant of APR. Aralia cordata, which is difficult to distinguish from A. continentalis, and Heracleum moellendorffii, which is frequently used in lieu of A. continentalis, are traded in Korean herbal markets. In contrast, in China, Angelica pubescens is recognized as the source plant of APR. In this study, we devised a method not only to discriminate A. contientalis from A. cordata, but also to discriminate both A. contientalis and A. cordata from H. moellendorffii and A. pubescens. Based on the discrepancy in the sequences of specific regions of ITS, we designed a Cont F/ Cont R primer set to amplify a 173 bp PCR band that appears only in A. continentalis. Additionally, we designed an Ara F/ Ara R primer set to amplify a 278 bp PCR band that appears in both A. continentalis and A. cordata. Using these primer sets and the ST R primer to confirm the PCR amplification results, we developed a simple multiplex PCR method for differentiating A. continentalis from A. cordata and to concurrently differentiate both A. continentalis and A. cordata from other APR herbs.

• Food Science and Preservation
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• v.17 no.5
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• pp.602-607
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• 2010

The quality characteristics and shelf life of wet noodles containing a freeze-dried powder of Heracleum moellendorffii(HM) were studied to investigate the use of HM as a food ingredient. The weight, volume, and water absorption of wet noodle sprepared with HM(HML) decreased as the concentration of HM increased. The turbidity of HML sauce and the loss in the solid content of noodles after cooking were lower than those of control noodles. Whiteness(L) and redness(a) values of wet noodles decreased but yellowness(b) increased after addition of HM. The sensory qualities of HML, including appearance, taste, color, flavor, texture, and overall acceptability, were better than those of control noodles. Addition of HM to 0.6%(w/w) afforded the best sensory qualities with respect to taste, texture, and overall acceptability. The total polyphenol content of HML increased as HM concentration increased. Noodle DPPH free-radical-scavenging activities were 22%(control), 28.41%(0.3%HM addition), 40.22%(0.6%HM addition) and 49.42%(0.9%HM addition). Viable bacterial cell counts did not differ significantly between control noodles and those prepared using 0.6%(w/w)HM during storage for 6day sat $10^{\circ}C$. However, viable cell numbers in noodles prepared using 0.9%(w/w) HM were significantly lower than those of control samples and of noodles prepared using either 0.3%(w/w) or 0.6%(w/w) HM, during storage for 12 days at $10^{\circ}C$. Changes in pH values showed trends similar to those of viable cell numbers during storage.

• Korean Journal of Pharmacognosy
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• v.31 no.1
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• pp.87-94
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• 2000

Mt. Dae-Am is the branch-range of DMZ located on the $38^{\circ}7'$ N KangWon-Do in South Korea. The resources of important Herbal medicine (medicinal plants) were Phacellanthus tubiflorus (fam.: Orobanchaceae), Ostericum maximowiczii, Dendranthemum zawadskii Herbich f. latifolium, D. zawadskii subsp. acutilobum, D. var. campanulatum, Halenia corniculata (fam.: gentianaceae), Prunus mandshurica var. glabra, Acanthopanax divaricatus f. inermis. A. chiisanense, A. sessiliflorum, Eleutherococcus senticosus, Bupleurum longeradiatum, Heracleum moellendorffii sub-spp. subbipinnatum, Sanicula rubriflora, Spuriopimpinella bracycarpa f. latifolia, Angelico gigas, Artemisia montana, A. stelleriana, Paeonia japonica, Phellodendron amurense, Schizandra chinensis, Menyanthes trifoliata, and Gentiana axillariflora var. coreana, etc.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.82-82
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• 2018

산채는 주로 산지에서 자생하는 것을 채취하거나 자생지와 비슷한 환경의 임간에서 재배하여 수확을 한다. 본 시험은 차광처리를 통해 산지가 아닌 노지에서 산채 재배 가능성을 검토하고자 하였다. 대상작목은 개미취(Aster tataricus), 눈개승마(Aruncus dioicus var. kamtschaticus), 단풍취(Ainsliaea acerifolia), 박쥐나물(Parasenecio auricudatus var. matsumurana), 산마늘(Allium microdictyon), 어수리(Heracleum moellendorffii), 우산나물(Syneilesis palmata)이다. 시험작목을 전북 남원시 허브시험장(표고 500m) 포장에서 2017년 4월에 종묘를 정식하여 약 2년간 재배하였다. 고온기인 5월부터 9월까지 35%, 55%, 75%, 95% 차광정도를 달리하여 설치 한 후 시설 내의 기온, 상대습도 등 환경조건 및 재배작물의 고사율, 출현율, 생육특성 등을 조사하였다. 시설 내부의 평균 기온은 차광정도가 높아질수록 다소 낮아졌으며, 상대습도는 점차 높아졌다. 차광정도별 고사율을 조사한 결과 개미취, 어수리, 우산나물의 경우는 차광비율에 상관없이 각각 3.6%, 5.7%, 9.8%로 낮게 나타났다. 반면 단풍취와 산마늘의 경우 차광 비율에 따른 편차가 크지는 않았지만 최대 고사율이 각각 20.0%, 36.8%로 다소 높게 나타났다. 또한 눈개승마 75% 차광에서 50.0%, 박쥐나물 35% 차광에서 100% 고사율을 보여 적정 차광비율 선정 필요하다고 판단된다. 정식 2년차 4월 평균 출현율은 개미취 100%, 눈개승마 86.2%, 단풍취 84.3%, 박쥐나물 77.9%, 산마늘 97.5%, 어수리 88.9%, 우산나물 96.3%이었다. 다만 단풍취, 박쥐나물은 차광정도가 높아질수록 출현율이 높아졌고, 어수리는 차광정도가 높아질수록 다소 출현율이 낮아졌다. 또한 생육특성 조사 결과 초장은 단풍취를 제외하고 차광처리별 경향성이 뚜렷하게 나타나지 않았으며, 엽장과 엽폭은 산마늘을 제외한 작목들은 차광이 강할수록 커지는 경향이었다. SPAD 값은 개미취와 산마늘은 처리구역별 차이가 없었으나, 나머지 작물은 차광이 높아질수록 SPAD 값도 커졌다. 시험결과 표고 500m지역에서 개미취, 산마늘, 어수리, 우산나물은 차광수준에 따른 생육의 차이가 크지 않거나 차광이 낮아도 생육이 비교적 양호하였다. 그러나 단풍취와 박쥐나물은 차광정도에 따라 생육의 차이가 분명하였으며, 차광이 높아질수록 생육이 좋아졌다. 이를 바탕으로 산채류 노지재배를 위해서는 연차 간 작목별 생육특성, 수량성, 상품율, 경제적 분석 등의 추가적인 조사가 이루어져 할 것으로 여겨진다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.53-53
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• 2018

전라북도 동부권 지역은 중산악지형으로 표고 100~500m 산지가 69%, 500~1,000m가 26%로 이루어져 있어, 산채를 재배하기에 유리한 지리적 조건을 갖고 있다. 따라서 소비자의 수요가 높은 산채류의 재배 및 이용에 대한 농업인들의 기술개발이 요구되고 있다. 이에 전라북도 준고랭지역에서 재배하기 적합한 산채작목을 선발하고자 본 시험을 실시하였다. 시험은 표고 500m 전라북도 남원시 운봉읍 허브시험장에서 노지재배, 남원시 주천면 표고 530~540m, 진안군 주천면 표고 340~350m에 위치한 농가포장에서 임간재배를 실시하였다. 대상작물은 개미취(Aster tataricus), 갯기름나물(Peucedanum japonicum), 곤달비(Ligularia stenocephala), 눈개승마(Aruncus dioicus var. kamtschaticus), 단풍취(Ainsliaea acerifolia), 산마늘(Allium microdictyon), 섬쑥부쟁이(Aster glehnii), 어수리(Heracleum moellendorffii), 우산나물(Syneilesis palmata) 등 9종을 2016년 종묘를 정식하였다. 정식 2년차에 해당지역의 5월~9월 평균기온 및 조도 등 생육환경을 조사하고, 시험작물의 출현율, 생육특성, 수량 등을 조사하였다. 조사기간 동안의 평균기온은 노지가 $20.8^{\circ}C$, 남원임간은 $20.3^{\circ}C$, 진안임간은 $20.5^{\circ}C$이었으며, 평균조도는 노지가 37,362Lux, 남원임간은 7,816Lux, 진안임간은 25,316Lux이었다. 노지에서 출현율은 개미취, 갯기름나물, 눈개승마, 산마늘, 섬쑥부쟁이, 우산나물이, 임간에서는 개미취, 곤달미, 눈개승마, 단풍취, 산마늘, 어수리, 우산나물이 높았다. 생육특성은 노지에서 개미취, 갯기름나물, 눈개승마, 섬쑥부쟁이, 임간에서는 단풍취, 산마늘, 우산나물의 생육이 좋았다. 수량은 노지에서 개미취, 갯기름나물, 곤달비, 산마늘, 섬쑥부쟁이, 어수리가 높았으며, 남원임간에서는 곤달비, 단풍취, 우산나물, 어수리가, 진안임간에서는 개미취, 갯기름나물, 산마늘, 우산나물의 수량이 많았다. 이와 더불어 준고랭지에 적합한 산채류률 선발하기 위해서는 차후 재배 지역 및 방법의 차이에 따른 산채작물의 연차별 수량성과 경제성에 관한 연구가 이루어져야 할 것으로 여겨진다.