• Journal of Aquaculture
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• v.15 no.3
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• pp.145-155
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• 2002

Effect of density (30, 40, 65, 90 or 120/cage) of lantern and bottom cages on growth of the ark shell, Scapharca satowi was studied in the Korean west coast from April 2000 to October 2001, when the following range of environmental conditions prevailed : temperature : 4.2 -25.5 $^{\circ}C, salinity : 30.23-32.$15\textperthousand, dissolved oxygen : 5.12-7.16 $ml$/l, pH : 7.84-8.17, phosphate : 0.22-0.56 $\mu $M, dissolved inorganic nitrogen : 3.16-9.10 $\mu $M, suspended solid : 7.6-17.9 mg/l, chemical oxygen demand: 0.46-1.61 mg/l and chlorophyll-a : 0.92-5.93 $\mug/l. Daily growth rate of shell length ranged from 0.066 to 0.071 mm/day for the lantern net cages, and from 0.079 to 0.082 mm/day for the bottom cages. Total weight also ranged from 0.067 to 0.082 g/day in the lantern net cages, as against 0.099 to 0.114 g/day in the bottom cages. Hemoglobin content of S. satowi (55 mm shell length), which was about 3.9 g/dl during february, 2001, increased to 6.0 and 7.0 g/dl during October, 2001 in animals culture in the lantern and bottom cages, respectively. ANOVA test of the growth rate showed that the growth rate of S. satowi, was significantly dependent on rearing density and the tested culture methods (P < 0.0001). The daily growth rate of the shell length was more significantly correlated with water temperature; the growth rate of shell length and total weight showed a tendency decrease with decreasing temperature. In cages suspended at the bottom, not only the increase shell weight but also the meat obtainable from comparable sized S. satowi was greater. Survival decreased with increasing density and was optimal at the density of 30 individual/cage.

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
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• 1999.06a
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• pp.97-117
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• 1999

This study was performed to investigate the effect of pork on the cadmium detoxification in rats. Ninety male Sprague-Dawley rats weighing $125.3{\pm}1.4g$ were divided into five groups based on cadmium treatment(0, 25, 50, 100, 250ppm) and five levels of Cd in AIN-76 purified diet had been fed for 8 weeks. Cadmium was supplemented with a form of $CdCl_2$.. During following 8 weeks of intoxication, casein was replaced by pork and the effect of pork on cadmium- detoxification was compared with casein. After 8 weeks of Cd intake had resulted in apparent cadmium intoxication; reduced growth rate, enlarged kidney and testis, decreased hematocrit value and hemoglobin content in response to the supplemented Cd levels in the diets. Discontinuing cadmium feeding, the body weights were relieved. Pork-fed groups seemed to have higher body weight than casein-fed groups. Hemoglobin content and hematocrit value became normal range at detoxification stage. The weights of liver, kidney, and testis were decreased along with cadmium intake. However, organ weight ratio was not affected by cadmium. Cadmium accumulation in liver and kidney showed a tendency to increase in the cadmium-exposed groups. The levels of metallothionein were also significantly elevated in the tissues of liver in response to the levels of Cd supplemented(p<0.05). Cadmium concentration in kidney was two times higher than that in liver. Cadmium removal rate of liver was higher than that of kidney. Cadmium accumulation of the pork-fed group was lower than that of casein. Especially, the factors which affected the cadmium contents in kidney were $Cd^{***}$ and $Cd{\times}pork^{***}$. Metallothionein(MT) was increased with cadmium, and MT was not likely to be affected by pork. Based on the findings from gross lesion, rats fed 250ppm of Cd were externally emaciated, had exposed penis and observed atrophies of kidney and testis. Histopathological observation seemed that the liver of groups feeding Cd supplemented diets showed cellular degeneration and accumulation of eosinophilic materials in the capillaries. In kidney, rats fed Cd diets had shown tubular epithelium degeneration and lesions of basophilic materials, while testis were weakened in numbers of spermatid and sporadically enlarged of giant cells. But the rats administered cadmium-detoxified diet supplemented pork for 7 weeks were shown individually decreased lesions compared with the rats supplied with casein diet.

• Journal of the Korean Society of Food Science and Nutrition
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• v.6 no.1
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• pp.41-47
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• 1977

This study was designed to evaluate the nutritive value of pearl barley. Forty males and the same number of females of albino rats, the Leuise strain, weighing from 52.1g to 59.1g were divided into eight experimental groups, five males and females were composed of one group. A group: casein 15%+starch 75%+oil 8%+vitamin mixture 1%+salt mixture 1% B group: pearl barley 98%+vitamin mixture 1%+salt mixture 1% C group: rice 98%+vitamin mixture 1%+salt mixture 1% D group: barley 98%+vitamin mixture 1%+salt mixture 1% E group: rice 49%+pearl barley 49%+vitamin mixture 1%+salt mixture 1% F group: rice 73.5%+pearl barley 24.5%+vitamin mixture 1% +salt mixture 1% G group: barley 49%+pearl barley 49%+vitamin mixture 1% +salt mixture 1% H group: barley 73.5%+Pearl barley 24.5%+vitamin mixture 1%+salt mixture 1% The rats were kept in an individual cage and were fed 8 different diets for 7 weeks and fed by ad-libitum feeding method. The results of this study were eluciated as followings. 1. B group was significantly lower in weight gain than the other groups. 2. Polished pearl barley showed higher digestion rate in protein and carbohydrate than brown pearl barley but lower in fat. 3. Liver weight was obserbed significant differences between B group and C group. but no significant differences between the others. 4. B group was significantly lower in the total protein content of liver and A/G ratio in plasma than other groups. 5. B group was also the lowest in the hematocrit and hemoglobin contents among the experimental groups. 6. Free amino acid composition in plasma was similar to those in diet.

• Korean Journal of Veterinary Research
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• v.27 no.2
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• pp.361-383
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• 1987

This thesis was designed to find out whether the cadmium content of hair in living animals reflects the status of cadmium accumulation in internal organs or muscles so that this status can be used as a diagnostic method. Forty-five rats were divided into 4 experimental groups and one control group. The control group received a basal diet which contained zinc $100{\mu}g/g$, iron $80{\mu}g/g$ and copper $135{\mu}g/g$. The experimental group received experimental diets sllpplemented with 1, 10, 100 or $1,000{\mu}g/g$ cadmium as cadmium sulfate. Rats were dissected on the 28th or 56th day of experimental periods and tissues, blood and hair samples were taken, dried and burned to ash and analysed. The cadmium, iron zinc and copper concentrations were measured with an atomic absorption spectrophotometer. In addition feed efficiency and hematological changes were observed. The results obtained can be summerized as follows: A marked decrease in feed intake, weight gain and feed efficiency were observed from 1 or 2 weeks of experimental periods, which was in accordance with the dosage and periods. The cadmium contents of kidney, liver, spleen and muscle were significantly increased in all experimental rats; the accumulation was marked in kidney and liver tissues. It was shown that the cadmium contents of hair reflects the cadmium accumulation in internal organs and muscle; the higher the cadmium levels of diet and of rat tissues, the higher the cadmium content of hair. In the $100{\mu}g/g$ group of rats erythrocyte counts and. hemoglobin concentrations were decreased. A significant(p<0.01, p<0.05) increase in iron contents of kidney, liver and muscle was observed in 10, 100, $1,000{\mu}g/g$ groups of rats on the 28th day of experiment. A significant (p<0.01, p<0.05) increase in zinc contents of kidney, liver and muscle was observed in all experimental rats. On the other hand, serum zinc concentration was decreased. A significant(p<0.01) increase in copper contents in the liver was observed on the 28th day in $100{\mu}g/g$ and $1,000{\mu}g/g$ groups of rats. From these results it may be concluded that the analysis of hair for cadmium is a useful diagnostic approach for the cadmium status of internal organs and muscle in living animals.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.12
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• pp.1732-1737
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• 2003

An experiment was conducted to investigate the effect of feeding transgenic cottonseed (Bt.) vis-a-vis non-transgenic (non-Bt.) cottonseed on blood biochemical constituents in lactating Murrah buffaloes. Twenty Murrah buffaloes in mid-lactation were divided into 2 groups of 10 each. Animals of group I were fed with 39.5% non-transgenic cottonseed in concentrate mixture while the same percentage of transgenic (Bt.) cottonseed was included in the concentrate mixture fed to the animals of group II. Animals of both groups were fed with concentrate mixture to support their milk production requirements. Each buffalo was also offered 20 kg mixed green fodder (oats and berseem) and wheat straw ad libitum. The experimental feeding trial lasted for 35 days. There was no significant difference in the dry matter intake between the two groups of buffaloes. All the buffaloes gained body weight, however, the differences were non significant. Total erythrocyte count, hemoglobin content and packed cell volume were $9.27{\pm}0.70${\times}10^6/{\mu}l$, $13.01{\pm}0.60gdl$ and $34.87{\pm}1.47%$, respectively in group I with the corresponding figures of $8.88{\pm}0.33$, $12.99{\pm}0.52$ and $31.08{\pm}1.52$ in group II. The values of total erythrocyte count, haemoglobin content and packed cell volume did not differ significantly between the two groups of buffaloes. The concentration of plasma glucose, serum total proteins, albumin, globulin, triglycerides and high density lipoprotein were non significantly higher in buffaloes fed non-transgenic cottonseed than in buffaloes fed transgenic cottonseed. The cholesterol concentration was significantly (p<0.01) higher in buffaloes of group I ($136.84{\pm}8.40mg/dl$) than in buffaloes of group II ($105.20{\pm}1.85mg/dl$). The serum alkaline phosphotase, glutamic-oxaloacetate transaminase and glutamic-pyruate transaminase activities did not differ significantly between two groups of buffaloes. However, serum glutamic-pyruate transaminase activity was considerably high in buffaloes fed nontransgenic cottonseed as compared to buffaloes fed transgenic cottonseed. Bt. proteins in serum samples of animals of group II were not detected after 35 days of feeding trial. It was concluded that transgenic cottonseed and non-transgenic cottonseed have similar nutritional value without any adverse effects on health status of buffaloes as assessed from haematobiochemical constituents.

• Toxicological Research
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• v.23 no.4
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• pp.353-362
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• 2007

Deoxynivalenol (DON) is a common food borne mycotoxin and occurs predominantly in grains such as wheat, barley, oats, etc. DON induces systemic health problems such as loss of appetite, emesis and diarrhea in both human and farm animals. Reliable diagnostic parameters for DON intoxication are needed to prevent deep health impact. In order to establish useful diagnostic parameters, we investigated clinical signs, hematological values, serum biochemical values, gross-, histo- and toxico-pathological findings in B6C3F1 male mice after oral administration of DON (0.83, 2.5 and 7.5 mg/kg) for 8 days. Body weight gain was significantly decreased at the highest dose of DON. Anorexia, ataxia, for crudness and lack of vigor were observed at the highest dose DON group. In hematological values, the numbers of WBC and platelets and hemoglobin content were reduced with decreased neutrophil and monocytes by 7.5 mg/kg DON. Prothrombin time (PT) and activated partial thromboplastin time (aPTT) were prolonged in a dose-dependent manner and the content of fibrinogen was elevated at high dose of DON. Of serum biochemical values, total protein, globulin, BUN, cholesterol and test-osterone were reduced but total bilirubin and albumin/globulin ratio increased. The enzyme activity of alkaline phosphatase was decreased while that of alanine aminotransferase was elevated. Relative organ weights of thymus, seminal vesicle/prostate and testes were dose-dependently reduced but those of liver and left adrenal gland increased with dose dependency. As for pathological findings, atrophy of thymus, seminal vesicle/prostate and testes and submucosal edema and ulceration in stomach and depletion of lymphocytes in thymus cortex were observed. In conclusion, these clinical, hematological, blood biochemical and patholgical parameters obtained in the present studies can be used for diagnosis of DON-mycotoxicosis, especially, low WBC, platelets, protein, BUN and testosterone and delayed prothrombin time can be available as for reliable diagnostic parameters.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.47 no.6
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• pp.1063-1068
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• 2014

We investigated the effects of feeding rate on the growth performance and blood components of sub-adult olive flounder Paralichthys olivaceus. Optimum feeding rate (initial fish mean weight : $370{\pm}5.72g$) was determined under the low water temperature. Two replicated groups of fish were fed a commercial diet at rates of 0%, 0.3%, 0.4%, 0.5%, and 0.6% body weight (BW) per day, and to satiation (0.75%). Feeding trial was conducted under a flow-through system with 12 1.2-metric ton aquaria receiving filtered seawater at $12-14^{\circ}C$ for 4 weeks. Weight gain (WG) and specific growth rate (SGR) for fish fed at 0.6% BW per day was significantly higher than that of unfed fish (0%) and fish fed at 0.3% and 0.4% BW per day. There were no significant differences in WG and SGR among fish fed at 0.5%, 0.6%, and 0.75%. These parameters were negative and significantly lower in the starved fish than in fish fed the experimental diet at all feeding rates. Survival for unfed fish (0%) was significantly lower than that of fish fed at 0.4%, 0.5%, and 0.6%. Hematocrit and hemoglobin content of fish fed at 0% and 0.75% (satiation) were significantly lower than that of fish fed at 0.4% BW per day. Total protein content in unfed fish was significantly lower than those in other treatments. Broken-line regression analysis of weight gain showed that the optimum feeding rate of olive flounder weighing 370 g was 0.51% BW per day at the low water temperature.

• Journal of Aquaculture
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• v.13 no.1
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• pp.1-7
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• 2000

The effect of various dietary protein levels on growth and body composition of young common carp raised from 56 g to 170 g in recirculating system was investigated for 15 weeks when they were fed to visual satiety three times daily. Five experiemtal diets were formulated to contain 40, 35, 30, 25 and 21% protein levels and 3.56, 3.59. 3.63, 3.66 and 3.69 kcal/g diet GE levels respectively. Mean survival rates of the fish fed the 40, 35, 30 and 25% protein diets were not different but sig-nificantly higher than that of the fish fed the 40, 35, 30 and 25% protein diets were not different but sig-nificantly higher than that of o the fish fed the 21% protein diet(P<0.05) Weight gain (g/tank) of common carp fed the 30% protein diet was the best. However weight gain of the fish fed the 25, 30, 35 and 40% protein diets were not different but significantly better than that of the fish fed the 21% protein diet. Feed efficiency ratio of the 21% protein diet was significantly lower (P<0.05) than for other groups of diets which were not different among them. Protein efficiency ratio for the 21% protein diet was significantly lower (P<0.05) than for other groups of diets which were not different among them(P>0.05) Dietary protein level had no effect on hemoglobin content in the fish(P>0.05) Crude protein contents of whole body of the fish fed the 35 and 40% protein diets were significantly higher than that of the fish fed the 21$$\mid$% or 25% protein diet(P<0.05) Body crude lipid contents of the fish fed the 21 and 25% protein diets were significantly higher than that of the fish fed the 30% or 35% protein diet. Crude ash contents of the fish fed the 35 and 40% protein diets were significantly higher than that of the fish fed the 21% or 25% protein diet(P<0.05) Moisture content of the fish fed the 35% protein diet was significantly higher than that of the fish fed the 21% protein diet(P<0.05) In considering growth performance of common carp and efficiency of diet dietary protein level could be lowered up to 25% without the reduction of young common carp production in recirculating system.

• Journal of Aquaculture
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• v.16 no.4
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• pp.267-272
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• 2003

This study investigated sex determination through serum steroid hormone level and compared hematological characteristics of one-year old cultured eels, Anguilla japonica. Four different groups were divided with total length at 40∼45, 45∼50, 50∼55, and 55∼60 cm. Males dominated eels of 40∼50 cm in total length group, while females dominated eels of more than 50 cm. All females and males were immature. Hematocrit levels of males (total length: 40∼50 cm) were higher than those of females (total length: 50∼60 cm). Hemoglobin content also differed between males and females. Glutamate oxaloacetate transaminase (GOT) levels of 40∼45 cm males tended to be higher (157$\pm$3.46 IU/L) than that of other sized males, but there were no significant differences between groups (P<0.05). The highest GOT levels were found in of 55∼60 cm females (148$\pm$3.46 IU/L) compared with that of other female groups (P<0.05). Glutamate pyruvate transaminase (GPT) levels of males and females in the 55∼60 cm size range was significantly higher thanothers (P<0.05). The result of this study indicated steroid hormone content of males and females were very low, but testosterone (T) and estradiol-17$\beta$, (E2) contents of females were higher than those of males. For further research of sex determination in cultured eels, it needs to investigate in more defined body length.

• The Korean Journal of Physiology
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• v.2 no.2
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• pp.93-99
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• 1968

The Present study attempted to analyze the fate of CO diffused into the circulating blood through the alveoli. Dogs were induced to CO poisoning by rebreathing CO gas mixture contained in Krog's spirometer, by closed circuit method, for 60 minutes. The spirometer was filled initially with 282 ml of CO and 20 liters of air and oxygen, so the composition of gases were arranged as 1.4% in CO and 50% in $O_2$ at the begining of the rebreathing. Oxygen was added corresponding to the utilization of $O_2$ by the animal in proceeding of the experiment. At 60th minutes of CO rebreathing, the concentration of CO in arterial blood and in mixed venous blood were analysed and compared with each other after the CO contents were corrected with the hematocrit measured in the arterial and mixed venous blood. The distribution of CO gas to other tissues was estimated by the analysis of CO diffused into the cystic bile and into the peritoneal gas pocket which was formed by injection of 300 ml air into the peritoneal cavity prior to the CO gas rebreathing. The blood volume was measured by dilution method using $^{51}Chromium$ tagged red cells. CO amount vanished in the animal body was calculated by subtraction of total CO content in blood stream and the CO remained in closed circuit breathing system from the CO amount given to the breathing system at the begining of the experiment. Results obtained are summarized as follows: 1. The content of CO corrected by the hematocrit value was slightly less in mixed venous blood than in arterial blood. The amount of CO diffused into the cystic bile and into the peritoneal cavity was averaged to 0.1% and 0.4% of the CO amount in 100 ml of blood, respectively. 2. For 60 minutes of CO rebreathing, CO-hemoglobin saturation reached about 77% at the 60th minutes, CO amount vanished in the experimental animal averaged 36.1 ml/dog/hr., or 21% of the total CO volume in the blood stream. The average vanishing rate of CO during 60 minutes of CO rebreathing per kg of body weight was 2.71 ml/hr. Production of CO measured in ten dogs under hypoxic condition averaged 0.023 ml/kg/hr. The major part of the CO vanished in the dogs seemed to be oxidized to $CO_2$ by various tissues of the animal. The conclusion might be delivered as such oxidation of CO to $CO_2$ by animal tissues can play a role in part of the process of recovery and protection of animal from CO-poisoning.