• Journal of Life Science
• /
• v.18 no.6
• /
• pp.753-758
• /
• 2008

Genus Hemerocallis is a herbaceous species and some species among their taxa are very important herbal medicines. We evaluated representative samples of the eight taxa in Korea with inter simple sequence repeats (ISSR) markers to estimate phylogenetic relationships within taxa of this genus. The studied taxa were Hemerocallis fulva L., H. fulva for. kwanso, H. dumortieri Morren, H. coreana Nakai, H. hongdoensis M.G.Chung & S.S.Kang, H. middendorffi Trautv. et Mayer, H. thunbergii Baker, H. minor Miller. In addition, we investigated the genetic variation and structure of Korean populations of these taxa. The mean genetic diversity was 0.098 across species, varying from 0.068 to 0.123. A low level of genetic variation was found in populations of Hemerocallis species. Specially, gene diversity for H. minor was maintained the highest among genus Hemerocallis. An indirect estimate of the number of migrants per generation (Nm=0.218) indicated that gene flow was not extensive among Korean populations of Hemerocallis species. The phylogenic tree showed distinct three clades. One includes H. fulva, H. fulva for. kwanso and H. middendorffi. Another includes three Hemerocallis species, H. dumortieri, H. thunbergii and H. minor. The H. coreana and H. hongdoensis were shown as the sister group to the second clades. Although the size of sampling was not large enough for eight Korean Hemerocallis species, the analyses of ISSRs will certainly provide an enhanced view on the phylogeny of species.

• Mycobiology
• /
• v.32 no.2
• /
• pp.95-97
• /
• 2004

In July 2002, a destructive stem rot of tawny daylily(Hemerocallis fulva) was occurred sporadically in exhibition farm of Gyeongsangnam-do Agricultural Research and Extension Services located in Hamyang-gun, Korea. The fungus also caused collar and crown rot, and systemic wilt or blight of whole plant. White mycelium spread over stems and petioles of infected plants and sclerotia were formed on the old lesions and near the soil surface. The optimum temperature for mycelial growth and scierotial formations was $30^{\circ}C$ on PDA. The mycelial width ranged $4.2{\sim}10.4{\mu}m$ and the color was white, usually many narrow mycelial strand grew in the aerial mycelium and formed clamp connection. The shape of sclerotia was spherical and $1.0{\sim}3.2$ mm in diameter. The fungus was isolated repeatedly from the infected tissues and confirmed its pathogenicity to Hemerocallis fulva and identified as Sclerotium rolfsii. This is the first report on the stem rot of H. fulva caused by S. rolfsii in Korea.

• Journal of the Korean Society of Environmental Restoration Technology
• /
• v.11 no.3
• /
• pp.62-73
• /
• 2008

This paper was conducted to acquire the scientific data for the waste landfill greening. The native plant flora was investigated in waste landfill at Nanjido. As a result, total 40 species, 22 families, 32 genus were classified. The dominant species were Robinia pseudoacacia (15%), Populus euramericana (3%), and Populus monilifera (1%), ect. Naive plant of 6 species (Zoysia japonica, Aster koraiensis, Liriope platyphylla, Hemerocallis fulva, Sedum kantschaticum, Indigofera pseudotinctoria) were planted in waste landfill in one species per 1 $m^2$ for greening purpose. After One year planting, the ornamental optimum value was showed in Zoysia japonica, Aster koraiensis, Hemerocallis fulva, Hemerocallis fulva, Sedum kantschaticum. The covering rate after nine months planting was 85% and 80% for Zoysia japonica and Hemerocallis fulva, respectively. While Aster koraiensis(a) 13 %, Liriope platyphylla 8 % and Sedum kantschaticum appeared 22 % were obtained. Aster koraiensis (a) has highest height and Sedum kantschaticum was the shortest plant.

• The Journal of Korean Medicine
• /
• v.27 no.2 s.66
• /
• pp.122-133
• /
• 2006

Objectives : The amyloid $\beta$-protein ($A\beta$) is the principal component of the senile plaques characteristic of Alzheimer's disease (AD) and elicits a toxic effect on neurons in vitro and in vivo. Many environmental factors including antioxidants and proteoglycans modify $A{\beta}toxicity$. In this study, we have investigated the protective effects of water- and organic solvent-extracts of Hemerocallis fulva root fractions pre-extracted with methanol on $A\beta$-induced oxidative cell death in cultured rat pheochromocytoma (PC12) cells. Methods : For this study, we used MTT reduction assay for detection of protective effects of water- and organic solvent-extracts of Hemerocallis fulva root fractions pre-extracted with methanol on $A{\beta}_{25-35}$-induced cytotoxicity to PC12 cells. We also used cell-based $\beta$-secretase assay system to investigate the inhibitory effect of water- and organic solvent-extracts of Hemerocallis fulva root on $\beta$-secretase activity. Results : We previously reported that methanol extracts of Hemerocallis fulva root strongly attenuated cytotoxicity induced by the three $A\beta$ fragments ($A{\beta}_{25-35},\;A{\beta}_{1-42}\;A{\beta}_{1-43}$) to both SK-N-MC and PC12 cells. In the present study, we found that butanol-, ethylacetate-, chloroform-, and water-extracts of Hemerocallis fulva root fractions pre-extracted with methanol had strong protective effects against $A{\beta}_{25-35}$-induced cytotoxicity to PC12 cells and inhibitory potency to $\beta$-secretase activity. Conclusion : These results suggest that butanol-, ethylacetate-, chloroform-, and water-extracts of Hemerocallis fulva root fractions pre-extracted with methanol may contain the protective component(s) against $A\beta$-induced cell death in PC12 cells as well as inhibitory component(s) to $\beta$-secretase activity.

• The Plant Pathology Journal
• /
• v.17 no.5
• /
• pp.305-307
• /
• 2001

In March 2000, gray mold was found on day lily (Hemerocallis fulva L.) in Korea. Among the symptoms observed was blight or early rot with chlorotic halo of the leaves. All the isolates obtained from the lesions of the diseased plant parts were identified as Botrytis elliptica, based on the morphological characteristics of conidia. Conidia that formed on conidiogenous cells were not in chains, hyaline to pale brown, unicellular, ellipsoidal to obovate with a single hilum at the base, entirely verruculose, and 21-31 x 12-$23\mu\textrm{m}$ in size. Pathogenicity of the fungus was established by artificial inoculation on day lily plants. This is the first record of gray mold on day lily caused by B. elliptica in Korea.

• Journal of Bio-Environment Control
• /
• v.16 no.1
• /
• pp.7-12
• /
• 2007

This study was conducted to clarify the effects of the different concentration of the nutrient solution on the early growth and the nutritional contents of hydroponically grown Aster koraiensis, Hemerocallis fulva and Plantago asiatica at 70 days after transplanting in perlite culture. Balanced nutrient solution formulated by Japanese Horticultural Experiment Station was used as the standard concentration of the nutrient solution. Overall plant growth of Aster koraiensis and Hemerocallis fulva such as plant height, stem diameter, number of loaves, fresh and dry shoot and root weight were significantly increased in 1.5 times concentration of nutrient solution. Shoot and root fresh and dry weight of hydroponically grown Plantago asiatica were significantly increased in the higher concentration of the nutrient solution, however, number of loaves and root length were significantly increased in the standard and the lower concentration of the nutrient solution. The highest contents of calcium, magnesium and sodium in plants were shown in Aster koraienxis which were grown in the 1.5 times concentration of nutrient solution, and Hemerocallis fulva and Plantago asiatica in 0.25 times of that. The contents of potassium in Aster koraiensis and Hemerocallis fulva were significantly increased when the plants were grown in the 0.5 times concentration of the nutrient solution, and that in Plantago asiatica in the 1.5 times concentration of the nutrient solution. The contents of phosphoric acid in plants as affected by the different species of Namul and the different concentration of the nutrient solution were not significant.

• Journal of Life Science
• /
• v.23 no.7
• /
• pp.847-853
• /
• 2013

The genus Hemerocallis (family Xanthorthoeaceae) is a herbaceous species, some of which are very important in herbal medicines. We evaluated the rps16-trnK region of the chloroplast DNA of a representative sample of eight taxa in Korea to estimate phylogenetic relationships within the taxa of this genus. Due to differences in the number of inserted nucleotides, the aligned data for Hemerocallis ranged from 729 (H. aurantiaca) to 742 nucleotides (H. fulva var. kwanso), with a mean of 736. Although several small indels and 20 inserts were present, sequence variation within the Hemerocallis genus was mostly due to nucleotide substitutions. All rps16-trnK trees generated in Korea exhibited a well-solved topology, with high bootstrap support, irrespective of the methods (parsimony) and the setting used. The node of H. minor and H. littorea was strongly supported, with a high bootstrap value in three trees, and these two taxa were sistered with H. thunbergii. The number of chromosomes was not congruent with that found in a previous study with RAPD, but the number was in agreement with the results of this study.

• Korean Journal of Pharmacognosy
• /
• v.33 no.2 s.129
• /
• pp.105-109
• /
• 2002

Besides chrysophanol and friedelin, mixtures of n-hydrocarbons [pentacosane (72.6%), heptacosane (14.6%), tetracosane (5.8%), nonacosane (4.1 %) and hexacosane (2.9%)], n-hydrocarbon alcohols [octacosanol (70.5%) and hexacosanol (29.5%)], l-monoacyl glycerols [acyl part; behenic acid (43.5%), lignoceric acid (32.4%), cerotic acid (9.3%), tricosanoic acid (8.9%), pentacosanoic acid (2.6%), octacosanoic acid (2.3%), heneicosanoic acid (1.0%)], wax esters [behenic acid (56.3%), lignoceric acid (23.0%), cerotic acid (19.8%), tricosanoic acid (4.6%), octacosanoic acid (4.0%), pentacosanoic acid (1.7%), triacontanoic acid (0.6%)/ octacosanol (33.7%), hexacosanol (21.0%), tetracosanol (15.6%), triacontanol (10.5%), docosanol (6.0%), tricosanol (6.0%), heptacosanol (4.2%), nonacosanol (3.0%)] and sterols [${\beta}-sitosterol$ (73.2%), stigmasterol (14.6%), campesterol (12.2%)] were isolated from the roots of Hemerocallis fulva. The acid : alcohol combinations of the major wax esters were $C_{48}$: 22 : 26, 24 : 24, $C_{46}$: 22 : 24, and $C_{44}$: 22 : 22. This is the first report of l-monoacyl glycerols and friedelin from this plant. All isolates were identified on the basis of spectral data and chemical reactions.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2019.09a
• /
• pp.154-154
• /
• 2019

• Korean Journal of Organic Agriculture
• /
• v.24 no.4
• /
• pp.787-795
• /
• 2016

The purpose of this study was to assess drought tolerance of groundcover plants for extensive green roof. Form July to November 2013, plant height, chlorophyll content, soil moisture, evapotranspiration and survival rate was measured. As results, after unirrigation started, Aster koraiensis reached 0% of soil moisture earliest followed by Sedum kamtschaticum, Hemerocallis fulva, Hylotelephium spectabile, Iris sanguinea, Hosta longipes and control. Among the plants, A. koraiensis withered earliest on 19th day of unirrigation experiment, followed by H. longipes and H. fulva (27th day); and I. sanguinea (29th day). S. kamtschaticum and H. spectabile survived even after 120th day of the experiment. As for chlorophyll content, I. sanguinea showed constant values in early stage than rapidly decreased right before its withering. Chlorophyll content of A. koraiensis and H. fulva showed rapid decrease from the beginning, while that of H. spectabile and S. kamtschaticum showed continuous reduction for first 30 days and the reduction was slowed down since then. In conclusion, H. longipes, I. sanguinea and H. fulva were found most applicable for extensive green roof considering scenic aspect.