• Journal of Life Science
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• v.18 no.6
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• pp.753-758
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• 2008

Genus Hemerocallis is a herbaceous species and some species among their taxa are very important herbal medicines. We evaluated representative samples of the eight taxa in Korea with inter simple sequence repeats (ISSR) markers to estimate phylogenetic relationships within taxa of this genus. The studied taxa were Hemerocallis fulva L., H. fulva for. kwanso, H. dumortieri Morren, H. coreana Nakai, H. hongdoensis M.G.Chung & S.S.Kang, H. middendorffi Trautv. et Mayer, H. thunbergii Baker, H. minor Miller. In addition, we investigated the genetic variation and structure of Korean populations of these taxa. The mean genetic diversity was 0.098 across species, varying from 0.068 to 0.123. A low level of genetic variation was found in populations of Hemerocallis species. Specially, gene diversity for H. minor was maintained the highest among genus Hemerocallis. An indirect estimate of the number of migrants per generation (Nm=0.218) indicated that gene flow was not extensive among Korean populations of Hemerocallis species. The phylogenic tree showed distinct three clades. One includes H. fulva, H. fulva for. kwanso and H. middendorffi. Another includes three Hemerocallis species, H. dumortieri, H. thunbergii and H. minor. The H. coreana and H. hongdoensis were shown as the sister group to the second clades. Although the size of sampling was not large enough for eight Korean Hemerocallis species, the analyses of ISSRs will certainly provide an enhanced view on the phylogeny of species.

• Korean Journal of Plant Taxonomy
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• v.42 no.4
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• pp.294-306
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• 2012

Taxonomic study of the genus Hemerocallis (Liliaceae) in Korea was conducted based on morphological data. H. middendorffii was distinguished from its related taxon H. dumortieri by the trait of coriaceous long bract with green color. H. taeanensis was distinguished from its related taxon H. minor by its odorless flowers, longer perianth tube and leaf length. H. thunbergii was distinguished from its related taxon H. lilioasphodelus by its long perianth tube. Lastly, H. hakuunensis was distinct from its related taxon H. hongdoensis as a result of its extreme branching of scape and short leaf width. From this study, it was revealed that H. minor, H. lilioasphodelus, and H. dumortieri were distributed only in the northern part of the Korean peninsular.

• Mycobiology
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• v.32 no.2
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• pp.95-97
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• 2004

In July 2002, a destructive stem rot of tawny daylily(Hemerocallis fulva) was occurred sporadically in exhibition farm of Gyeongsangnam-do Agricultural Research and Extension Services located in Hamyang-gun, Korea. The fungus also caused collar and crown rot, and systemic wilt or blight of whole plant. White mycelium spread over stems and petioles of infected plants and sclerotia were formed on the old lesions and near the soil surface. The optimum temperature for mycelial growth and scierotial formations was $30^{\circ}C$ on PDA. The mycelial width ranged $4.2{\sim}10.4{\mu}m$ and the color was white, usually many narrow mycelial strand grew in the aerial mycelium and formed clamp connection. The shape of sclerotia was spherical and $1.0{\sim}3.2$ mm in diameter. The fungus was isolated repeatedly from the infected tissues and confirmed its pathogenicity to Hemerocallis fulva and identified as Sclerotium rolfsii. This is the first report on the stem rot of H. fulva caused by S. rolfsii in Korea.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.11 no.3
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• pp.62-73
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• 2008

This paper was conducted to acquire the scientific data for the waste landfill greening. The native plant flora was investigated in waste landfill at Nanjido. As a result, total 40 species, 22 families, 32 genus were classified. The dominant species were Robinia pseudoacacia (15%), Populus euramericana (3%), and Populus monilifera (1%), ect. Naive plant of 6 species (Zoysia japonica, Aster koraiensis, Liriope platyphylla, Hemerocallis fulva, Sedum kantschaticum, Indigofera pseudotinctoria) were planted in waste landfill in one species per 1 $m^2$ for greening purpose. After One year planting, the ornamental optimum value was showed in Zoysia japonica, Aster koraiensis, Hemerocallis fulva, Hemerocallis fulva, Sedum kantschaticum. The covering rate after nine months planting was 85% and 80% for Zoysia japonica and Hemerocallis fulva, respectively. While Aster koraiensis(a) 13 %, Liriope platyphylla 8 % and Sedum kantschaticum appeared 22 % were obtained. Aster koraiensis (a) has highest height and Sedum kantschaticum was the shortest plant.

• The Journal of Korean Medicine
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• v.27 no.2 s.66
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• pp.122-133
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• 2006

Objectives : The amyloid $\beta$-protein ($A\beta$) is the principal component of the senile plaques characteristic of Alzheimer's disease (AD) and elicits a toxic effect on neurons in vitro and in vivo. Many environmental factors including antioxidants and proteoglycans modify $A{\beta}toxicity$. In this study, we have investigated the protective effects of water- and organic solvent-extracts of Hemerocallis fulva root fractions pre-extracted with methanol on $A\beta$-induced oxidative cell death in cultured rat pheochromocytoma (PC12) cells. Methods : For this study, we used MTT reduction assay for detection of protective effects of water- and organic solvent-extracts of Hemerocallis fulva root fractions pre-extracted with methanol on $A{\beta}_{25-35}$-induced cytotoxicity to PC12 cells. We also used cell-based $\beta$-secretase assay system to investigate the inhibitory effect of water- and organic solvent-extracts of Hemerocallis fulva root on $\beta$-secretase activity. Results : We previously reported that methanol extracts of Hemerocallis fulva root strongly attenuated cytotoxicity induced by the three $A\beta$ fragments ($A{\beta}_{25-35},\;A{\beta}_{1-42}\;A{\beta}_{1-43}$) to both SK-N-MC and PC12 cells. In the present study, we found that butanol-, ethylacetate-, chloroform-, and water-extracts of Hemerocallis fulva root fractions pre-extracted with methanol had strong protective effects against $A{\beta}_{25-35}$-induced cytotoxicity to PC12 cells and inhibitory potency to $\beta$-secretase activity. Conclusion : These results suggest that butanol-, ethylacetate-, chloroform-, and water-extracts of Hemerocallis fulva root fractions pre-extracted with methanol may contain the protective component(s) against $A\beta$-induced cell death in PC12 cells as well as inhibitory component(s) to $\beta$-secretase activity.

• Journal of Life Science
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• v.23 no.7
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• pp.847-853
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• 2013

The genus Hemerocallis (family Xanthorthoeaceae) is a herbaceous species, some of which are very important in herbal medicines. We evaluated the rps16-trnK region of the chloroplast DNA of a representative sample of eight taxa in Korea to estimate phylogenetic relationships within the taxa of this genus. Due to differences in the number of inserted nucleotides, the aligned data for Hemerocallis ranged from 729 (H. aurantiaca) to 742 nucleotides (H. fulva var. kwanso), with a mean of 736. Although several small indels and 20 inserts were present, sequence variation within the Hemerocallis genus was mostly due to nucleotide substitutions. All rps16-trnK trees generated in Korea exhibited a well-solved topology, with high bootstrap support, irrespective of the methods (parsimony) and the setting used. The node of H. minor and H. littorea was strongly supported, with a high bootstrap value in three trees, and these two taxa were sistered with H. thunbergii. The number of chromosomes was not congruent with that found in a previous study with RAPD, but the number was in agreement with the results of this study.

• The Plant Pathology Journal
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• v.17 no.5
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• pp.305-307
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• 2001

In March 2000, gray mold was found on day lily (Hemerocallis fulva L.) in Korea. Among the symptoms observed was blight or early rot with chlorotic halo of the leaves. All the isolates obtained from the lesions of the diseased plant parts were identified as Botrytis elliptica, based on the morphological characteristics of conidia. Conidia that formed on conidiogenous cells were not in chains, hyaline to pale brown, unicellular, ellipsoidal to obovate with a single hilum at the base, entirely verruculose, and 21-31 x 12-$23\mu\textrm{m}$ in size. Pathogenicity of the fungus was established by artificial inoculation on day lily plants. This is the first record of gray mold on day lily caused by B. elliptica in Korea.

• Journal of the Korean Society of Food Science and Nutrition
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• v.42 no.10
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• pp.1638-1648
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• 2013

To promote the utilization of wild edible plants, this study examined blanching, drying, and fermentation as methods for enhancing the functionality of Hemerocallis coreana Nakai. Specimens fermented for 24 hours at a fermentation temperature of $50^{\circ}C$, with a relative humidity of 65%, contained the highest amount of organic acid (18,109.82 mg/100 g). For the blanched; specimens, total organic acid content decreased about 30% compared with the freeze-dried specimens. The main organic acid of Hemerocallis coreana Nakai was confirmed as succinic acid. After fermentation, free sugars decreased; in particular, specimens fermented at a relative humidity of 80% showed a 32~75% reduction in free sugar compared with the freeze-dried specimens. In terms of amino acid content, Hemerocallis coreana Nakai was mainly composed of valine, isoleucine, and phenylalanine. In fermented specimens the total amino acid content was highest in a moderately fermented (17 hr) specimen, (1,010.71 mg/100 g fresh wt.), but decreased in the maximally fermented (24 hr) specimen. The longer the fermentation, the higher the decrease in non-essential amino acids content, while the content of more essential amino acids consistently increased. In conclusion, since seasoned Hemerocallis coreana Nakai contains a considerable amount of glutamine and asparagine, it has a fresh sour and sweet taste; thus, it will likely be a highly preferred wild edible plant. Also, with an increase of essential amino acids after fermentation, Hemerocallis coreana Nakai is excellent in terms of nutrition. Thus, it may be possible to utilize fermented Hemerocallis coreana Nakai in the development of diverse products.

• Korean Journal of Environment and Ecology
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• v.26 no.6
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• pp.868-875
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• 2012

This study was carried out to analyze the vegetation properties, soil characteristics and ordination of Hemerocallis hongdoensis population in South Korea. The Hemerocallis hongdoensis population was classified into Mallotus japonicus dominant population, Viburnum wrightii dominant population, Melampyrum setaceum dominant population. Hemerocallis hongdoensis were mainly distributed along the coast of south-western iland of the Korean penninsula and it's population was located at an elevation of 6m to 362m. In the study sites, soil organic matter, total nitrogen, exchangeable potassium, exchangeable calcium, exchangeable magnesium, cation exchange capacity and soil pH were 16.18~21.70%, 0.56~0.97%, 0.42~0.88mg/kg, $3.38{\sim}5.65cmol^+/kg$, $1.12{\sim}2.38cmol^+/kg$, $25.93{\sim}41.45cmol^+/kg$, and 4.45~4.86 respectively. Mallotus japonicus dominant population was found in the steep sloped area that has high percentage of cation exchange capacity and total nitrogen than other populations. Viburnum wrightii dominant population was found gentle sloped area that has low percentage of cation exchange capacity and total nitrogen. Melampyrum setaceum dominant population was found in the medium sloped area that has medium percentage of cation exchange capacity and total nitrogen. Current status of Hemerocallis hongdoensis habitats is very vulnerable with local development constantly threatening the species' survival. Thus, concrete conservation plans to protect natural habitats should be set up as soon as possible.

• Horticultural Science & Technology
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• v.34 no.6
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• pp.830-839
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• 2016

Germination of Hemerocallis seeds as influenced by cold stratification at $5^{\circ}C$ (CS) and maturity of seeds evaluated using X-ray imaging has not been well investigated. Seeds of H. lilioasphodelus, H. citrina, H. citrina 'April Flower', and H. minor collected from China and H. thunbergii collected from Korea were germinated at $20^{\circ}C$ without pre-temperature treatment, while H. hongdoensis, H. dumortieri, H. minor, and H. vespertina seeds were treated with CS. Harvesting 'Stella de Oro' capsules at 35-40 days after anthesis yielded mature seeds with well-developed embryo and cotyledons analyzed by X-ray images with a 92% germination in 17 days after sowing. Seeds of H. thunbergii and H. citrina germinated in less than 13 days without CS; two weeks of CS did not accelerate seed germination. Seeds of H. hongdoensis germinated in 24 days when seeds were stored at $25^{\circ}C$ without CS and in less than 27 days when cold stratified. Therefore, 'Stella de Oro' capsules should be harvested at 35-40 days after anthesis to harvest mature seeds. Cold stratification is not required to accelerate seed germination in the Hemerocallis taxa evaluated in this study.