• Biomolecules & Therapeutics
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• 제27권6호
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• pp.514-521
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• 2019

G protein-coupled receptors (GPCRs) are membrane receptors whose agonist-induced dynamic conformational changes trigger heterotrimeric G protein activation, followed by GRK-mediated phosphorylation and arrestin-mediated desensitization. Cytosolic regions of GPCRs have been studied extensively because they are direct contact sites with G proteins, GRKs, and arrestins. Among various cytosolic regions, the role of helix 8 is least understood, although a few studies have suggested that it is involved in G protein activation, receptor localization, and/or internalization. In the present study, we investigated the role of helix 8 in dopamine receptor signaling focusing on dopamine D1 receptor (D1R) and dopamine D2 receptor (D2R). D1R couples exclusively to Gs, whereas D2R couples exclusively to Gi. Bioinformatic analysis implied that the sequences of helix 8 may affect GPCR-G protein coupling selectivity; therefore, we evaluated if swapping helix 8 between D1R and D2R changed G protein selectivity. Our results suggest that helix 8 is not involved in D1R-Gs or D2R-Gi coupling selectivity. Instead, we observed that D1R with D2R helix 8 or D1R with an increased number of hydrophobic residues in helix 8 relative to wild-type showed diminished ${\beta}$-arrestin-mediated desensitization, resulting in increased Gs signaling.

• 한국인터넷방송통신학회논문지
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• 제16권5호
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• pp.229-233
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• 2016

본 논문에서는 4개의 유전자 핵염기 C, U(T), A, G를 행렬로 표시하고, $4{\times}4$ RNA(ribose nucleic acid)에서 $8{\times}8$ DNA(deoxyribose nucleic acid)로의 행렬 구조에 대해 서술한다. BCHJM (block circulant Hadamard-Jacket matrix)에 의해 DNA 이중나선 구조(double helix)를 해석한다. 직교 BCHJM은 비대칭 쌍 상보성(complementary)을 보이고 있다. 블록순환(block circulant) RNA 쌍 손상(damage) 신뢰성(reliability)은 기존 이중나선 보다 우수함을 보이고 있다. k=4, N=1인 경우 블록 순환 상보 쌍 신뢰도는 93.75%이고, k=4, N=4인 경우 신뢰도는 98.44%로 기존 이중나선의 경우 보다 4.69% 개선된다.

• 한류연구
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• 제2권1호
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• pp.1-8
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• 2023

The South Korean automotive industry has been the major producer for both domestic and international market. This rises of the South Korean automotive industry has resulted in combination various factors ranging from government policies and the foreign investment. The Double Helix model provides a comprehensive and useful framework to understand how these factors have interacted to shape the history of the auto industry of South Korea. This research aims to provide the history of the Auto Industry in Korea based on the Double Helix Model. To collect textual literature dataset, the present author tried to screen and select adequate prior studies in the past and current literature to achieve the purpose of the study. According to the literature analysis, the history of the automotive industry in South Korea is an interesting case in the successful implementation of the Double Helix model. The model emphasizes the collaboration between the government and the private sector in achieving common goals. This research founded the four historical events of the development of the South Korean automotive industry based on the Double Helix Models. In sum, the research concludes that the Double Helix Model is an essential tool for understanding the historical development of the auto industry in South Korea.

• Journal of Biosystems Engineering
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• 제41권1호
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• pp.12-20
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• 2016

Purpose: Pepper prices have risen continuously because of a decrease in cultivation area; therefore, mechanical harvesting systems for peppers should be developed to reduce cost, time, and labor during harvest. In this study, a screw type picking head for a self-propelled pepper harvester was developed, and the optimal working conditions were evaluated considering helix types, winding directions of helix, and rotational speeds of the helix. Methods: The screw type was selected for the picking head after analyzing previous studies, and the device consisted of helices and a feed chain mechanism for conveying pepper branches. A double helix and a triple helix were manufactured, and rotational speeds of 200, 300, and 400 rpm were tested. The device was controlled by a variable speed (VS) motor and an inverter. Both the forward and reverse directions were tested for the winding and rotating directions of the helix. An experiment crop (cultivar: Longgreenmat) was cultivated in a plastic greenhouse. The test results were analyzed using the SAS program with ANOVA to examine the relationship between each factor and the performance of the picking head. Results: The results of the double and triple helix tests in the reverse direction showed gross harvest efficiency levels of 60-95%, mechanical damage rates of 8-20%, and net marketable portion rates of 50-80%. The dividing ratio was highest at a rotational speed of 400 rpm. Gross harvest efficiency was influenced by the types of helix and rotational speed. Net marketable portion was influenced by rotational speed but not influenced by the type of helix. Mechanical damage was not influenced by the type of helix or rotational speed. Conclusions: Best gross harvest efficiency was obtained at a rotational speed of 400 rpm; however, operating the device at that speed resulted in vibration, which should be reduced.

• 한국전산유체공학회지
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• 제19권4호
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• pp.8-13
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• 2014

A series of numerical simulation has been carried out to study performance of a cyclone type oil separator, which is designed for the compressor of a refrigeration system. Working fluid is R22, which is a typical refrigerant, and mineral oil droplet is supplied. Pitch of the helix is considered as design parameters to make a compact separator. Depending on the helix pitch, separation efficiency varies from 97.5 to 99%, while predicted pressure drop ranges from 5 to 6.5 kPa. Considering both of the pressure drop and separation efficiency, helix pitch of the separator has been designed as 50 mm.

• BMB Reports
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• 제32권6호
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• pp.561-566
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• 1999

CA(1-8)-ME(1-12) [CA-ME], composed of cecropin A(1-8) and melittin(1-12), is a synthetic antimicrobial peptide having potent antibacterial and antitumor activities with minimal hemolytic activity. In order to investigate the effects of the flexible hinge sequence, Gly-Ile-Gly, of CA-ME on antibiotic activity, CA-ME and three analogues, CA-ME1, CA-ME2, and CA-ME3, were synthesized. The Gly-Ile-Gly sequence of Ca-ME was deleted in CA-ME1 and replaced with Pro and Gly-Pro-Gly in CA-ME2 and CA-ME3, respectively. CA-ME1 and CA-ME3 showed a significant decrease in antitumor activity and phospholipid vesicle-disrupting ability. However, CA-ME2 showed similar antitumor and vesicle-disrupting activities, as compared with CA-ME. These results suggest that the flexibility or ${\beta}$-turn induced by Gly-Ile-Gly or Pro in the central part of CA-ME may be important in the electrostatic interaction of the N-terminus cationic ${\alpha}$-helical region with the cell membrane surface and the hydrophobic interaction of the C-terminus amphipathic ${\alpha}$-helical region with the hydrophobic acyl chains in the cell membrane. CA-ME3 exhibited lower antitumor and vesicle-disrupting activities than CA-ME and CA-ME2. This result suggests that the excessive ${\beta}$-turn structure caused by the Gly-Pro-Gly sequence in CA-ME3 seems to interrupt ion channel/pore formation in the lipid bilayer. We concluded that the appropriate flexibility or bilayer. We concluded that the appropriate flexibility or ${\beta}$-turn structure provided by the central hinge is responsible for the effective antibiotic activity of the antimicrobial peptides with the helix-hinge-helix structure.

• Journal of Contemporary Eastern Asia
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• 제8권1호
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• pp.1-14
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• 2009

• Journal of Contemporary Eastern Asia
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• 제8권1호
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• pp.47-53
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• 2009

• Journal of Contemporary Eastern Asia
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• 제8권2호
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• pp.15-25
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• 2009

• Bulletin of the Korean Chemical Society
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• 제16권8호
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• pp.755-760
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• 1995