• 대한두개안면성형외과학회지
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• 제10권2호
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• pp.127-130
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• 2009

Purpose: In an amputated auricle, a microvascular anastomosis is the best treatment of choice. But, the neartotally amputated auricle which is connected by very narrow tissue pedicle to the head, can survive by simple attachment without a microvascular anastomosis owing to the rich vascular network through the superficial temporal artery and posterior auricular artery. In cases of venous congestion resulting from a lack of vein anastomosis, medical leeches (Hirudo medicinalis) can solve the problem. We are reporting the case of a 6-year-old boy who had a neartotally amputated auricle with successful results by simple closure and medical leech treatment without a microvascular anastomosis. Methods: A 6-year-old male patient had an left auricular injury by an escalator accident. The left auricle was neartotally amputated from the temporal head with connection only by very narrow skin and subcutaneous pedicle (about 1 cm in width) at the helical root of upper and anterior part of auricle. Marginal bleeding from the avulsed auricle was noted and the arterial blood was supplied from a branch of upper auricular branch of the superficial temporal artery. The auricle was repaired by simple closure including cartilage and skin without any vascular anastomosis. After simple closure, the auricle showed good circulation with pink color. But on the 2nd day after the operation, there was a venous congestion with severe swelling, which resulted in a purplish colored auricle. The venous congestion disappeared after using medical leeches by the 5th day after the operation. Results: The repaired auricle showed aesthetically and functionally satisfactory result with normal development at the 9 months follow-up check after the operation. Conclusion: In cases of neartotally amputated auricles of children or crushing injury in which microsurgery is difficult, we can try simple closure with the use of medical leeches in treating a of venous congestion for a successful result.

• BMB Reports
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• 제48권6호
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• pp.324-329
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• 2015

CopA3 is a homodimeric ${\alpha}$-helical peptide derived from coprisin which is a defensin-like antimicrobial peptide that was identified from the dung beetle, Copris tripartitus. CopA3 has been reported to have anticancer activity against leukemia cancer cells. In the present study, we investigated the anticancer activity of CopA3 in human gastric cancer cells. CopA3 reduced cell viability and it was cytotoxic to gastric cancer cells in the MTS and LDH release assay, respectively. CopA3 was shown to induce necrotic cell death of the gastric cancer cells by flow cytometric analysis and acridine orange/ethidium bromide staining. CopA3-induced cell death was mediated by specific interactions with phosphatidylserine, a membrane component of cancer cells. Taken together, these data indicated that CopA3 mainly caused necrosis of gastric cancer cells, probably through interactions with phosphatidylserine, which suggests the potential utility of CopA3 as a cancer therapeutic. [BMB Reports 2015; 48(6): 324-329]

• 한국지반공학회논문집
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• 제30권1호
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• pp.5-16
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• 2014

본 논문에서는 현장인발재하시험을 통한 스크류 앵커 파일의 인발 저항 특성에 관한 연구내용을 제시하였다. 현장시험에서는 동일한 피치를 가지나 샤프트나 스크류 직경이 다른 스크류 앵커 파일에 대해 다양한 시공조건을 도출하고 이에 대한 시험을 수행하였다. 실험결과 스크류 앵커 파일은 스크류가 없는 파일에 비해 현저히 큰 인발저항력을 발휘하는 것으로 검토되었으며 시간경과 효과와 그라우팅 여부에 따른 검토 결과 일반 파일과 유사하게 시간경과에 따라 인발저항력이 증가하는 셋업효과와 그라우팅시 인발저항력 상승효과를 기대할 수 있는 것으로 분석되었다. 이와 아울러 기존의 헬리컬 파일의 인발저항력 산정식을 스크류 앵커 파일의 인발저항력 평가에 있어서의 적용성을 검토하였으며 현장인발재하 시험결과를 다양한 측면에서의 상관관계를 파악할 수 있도록 제시하고 연구결과의 실무적 관점에서의 검토 내용을 기술하였다.

• Bulletin of the Korean Chemical Society
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• 제35권9호
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• pp.2809-2812
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• 2014

The 43-residue defensin-like peptide coprisin, which is isolated from dung bettle, Copris tripartitus, is a potent antimicrobial peptide. In our previous work, we determined the tertiary structure of coprisin and found that alpha helical region of coprisin from residue 19 to residue 30 is important for its antimicrobial activities. Here, we designed cop12mer and cop9mer analogs of coprisin based on the tertiary structure of coprisin. To investigate the relationship between hydrophobicity and antimicrobial activities and develop the potent peptide antibiotics, we designed cop9mer-1 with substitution of $His^2$ with Trp in cop9mer. The results showed that cop9mer-1 has higher toxicities as well as improved antimicrobial activities compared to cop9mer. In order to reduce the toxicity of cop9mer-1, we designed cop9mer-2 and cop9mer-3 with substitution of $Cys^3$ with Lys or Ser. Substitution of $Cys^3$ with these hydrophilic amino acids results in lower cytotoxicities compared to cop9mer-1. Cop9mer-2 with substitution of $Cys^3$ with Lys in Cop9mer-1 showed high antibacterial activities against drug resistant bacteria without cytotoxicity. Antibiotic action of cop9mer-1 analog appears to involve permeabilization of the bacterial cell membrane while cop9mer-2 and cop9mer-3 may have different mechanism of action. These results imply that that optimum balance in hydrophobicity and hydrophilicity in these 9-meric peptides plays key roles in their antimicrobial activities as well as cytotoxicities.

• Bulletin of the Korean Chemical Society
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• 제29권6호
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• pp.1233-1242
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• 2008

The hydration effect on the intrinsic magnetism of natural salmon double-strand DNA was explored using electron magnetic resonance (EMR) spectroscopy and superconducting quantum interference device (SQUID) magnetic measurements. We learned from this study that the magnetic properties of DNA are roughly classified into two distinct groups depending on their water content: One group is of higher water content in the range of 2.6-24 water molecules per nucleotide (wpn), where all the EMR parameters and SQUID susceptibilities are dominated by spin species experiencing quasi one-dimensional diffusive motion and are independent of the water content. The other group is of lower water content in the range of 1.4-0.5 wpn. In this group, the magnetic properties are most probably dominated by cyclotron motion of spin species along the helical π -way, which is possible when the momentum scattering time (${\tau}_k$) is long enough not only to satisfy the cyclotron resonance condition (${\omega}_c{\tau}_k$ > 1) but also to induce a constructive interference between the neighboring double helices. The same effect is reflected in the S-shaped magnetization-magnetic field strength (M-H) curves superimposed with the linear background obtained by SQUID measurements, which leads to larger susceptibilities at 1000 G when compared with the values at 10,000 G. In particular, we propose that the spin-orbital coupling and Faraday's mutual inductive effect can be utilized to interpret the dimensional crossover of spin motions from quasi 1D in the hydrate state to 3D in the dry state of dsDNA.

• 한국항행학회논문지
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• 제21권4호
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• pp.371-376
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• 2017

본 논문에서는 6.78 MHz 에서 공진하는 대형 코일을 이용한 자기공진방식 무선전력전송 시나리오를 제시하고 3차원 전자계 시뮬레이션, 자기공진방식 무선전력전송 등가회로를 통한 특성을 비교하였다. 본 논문에서 제시한 자기공진방식의 무선전력전송 등가회로는 기존 등가회로에 추가적으로 코일 간에 발생하는 기생 커패시턴스를 고려하였다. 이를 바탕으로 제작한 자기공진방식 무선전력전송 코일을 측정하고 시뮬레이션 예측과 비교하였다. 비교 결과 전송 특성 및 공진 주파수 천이 정도를 예측할 수 있었다. 제시한 자기공진방식 무선전력전송 등가회로 특성과 측정치의 오차는 ${\Delta}{\mid}S11{\mid}$이 최대 1.31 dB, ${\Delta}{\mid}S21{\mid}$이 최대 1.36 dB로 유사하게 예측하였다.

• 한국전기전자재료학회논문지
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• 제20권1호
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• pp.80-85
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• 2007

We report the electro-optical properties of the sensory rhodopsin II using a near-field scanning microwave microscope(NSMM). Rhodopsin was known as a photoreceptor pigment with a retinal as a chromophore via a protonated Schiff base and consists of seven ${\alpha}-helical$ transmembrane segments. The sensory rhodopsin II, expressing E. coli UT5600 with endogenous retinal biosynthesis system and purified with $Ni^{-2}-NTA$ affinity chromatography in the presence of 0.02 % DM (Dodecyl Maltoside) from Natronomonas pharaonis. We measured the absorption spectra and the transients difference of sensory rhodopsin II from Natronomonas pharaonis using a UV/VIS spectrophotometer with Nd-Yag Laser (532 nm). The absorption spectra of NpSR II showed a typical rhodopsin spectrum with a left shoulder region and the photointermediates spectra of NpSR II-ground state (${\lambda}max=498\;nm$), NpSR II-M state (${\lambda}max=390\;nm$), and NpSR II-O state (${\lambda}max=550\;nm$) during the photocycle. The observed photocycle reaction was confirmed by measuring the microwave reflection coefficient $S_{11}$ at an operating frequency of f=3.93-3.95 GHz and compared with the results of a photocycle of NpSR II.

• 한국해양공학회지
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• 제28권4호
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• pp.314-323
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• 2014

In this study, a gearbox and blade were modeled in the MASTA program, and the housing and carrier components were modeled using a finite element method. Using substructure synthesis, all the components were combined and used to establish a vibration model of a 2.5-MW wind turbine gearbox. In addition, the safety displacement factor was evaluated using an AGMA data sheet about bearing's outer race for the input shaft and output shaft. As a result, the bearing's outer race for the input shaft, and the radial and axial responses were satisfied by the $1^{st}$ and $2^{nd}$ planetary gears and the $3^{nd}$ helical gear transmission error(TE), respectively. However, the output shaft support bearing's outer race responses were not satisfied with the radial response by the $2^{nd}$ TE and axial response by the $3^{rd}$ TE. To reduce the vibration, tooth modification was needed. After profile tooth modification, at the outer race of the output shaft support bearing, the radial response was reduced by approximately $20{\mu}m$, and the axial response was reduced by approximately $6{\mu}m$.

• 한국전자파학회논문지
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• 제22권10호
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• pp.999-1004
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• 2011

본 논문에서는 무선 전력 전송 시스템에서 널리 사용되는 원형 코일 사이의 상호 인덕턴스 계산 방법과 이를 이용한 해석 결과를 제시한다. 두 원형 코일은 수직으로 배열되었다. 상호 인덕턴스 계산을 위해 수직 배열된 수전부 원형 코일의 면적을 일정한 높이의 단위 셀로 나누고, 각 단위 셀에 쇄교하는 자속 밀도를 구하여 상호인덕턴스를 계산하였다. 이를 위해 두 원형 코일을 필라멘트 코일로 모델링하였고, 각 코일에서의 전류는 균일 하다고 하였다. 검증을 위해 헬리컬, 스파이럴 형태로 두 원형 코일을 제작하였고, z 방향의 간격이 50~100 cm인 구간에서 상호 인덕턴스를 측정한 결과, 본 논문에서 제시한 이론값과 일치하였다.

• 한국기계가공학회지
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• 제19권9호
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• pp.116-121
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• 2020

In recent times, fuel economy enhancement and environmental regulation compliance have become the main topics of interest in the automobile industry. Electric vehicles are desirable alternatives to the existing cars that employ internal combustion engines. Specifically, electric vehicles are equipped with inverters, motors, and a gearbox instead of engines and transmission mechanisms. The gearbox is a key component, used to transmit power from the electric motor to the wheel. Therefore, the design of the gearbox is critical. However, most engineers design gears based only on their experience because no standards pertaining to the design factor exist, other than those for the gear ratios. To overcome this problem, the structural stabilities must be examined considering the design factors of the gears. In this study, we considered the module and number of teeth as the main factors. The constraints corresponded to the final gear ratio and fixed distance between each axle of the shafts. Moreover, a structural analysis was conducted, and the variation trend of the maximum equivalent stress against changes in the gear module and number of teeth was examined. By performing such an analysis, the structural stability in the design of a gear system could be effectively investigated.