• Proceedings of the Botanical Society of Korea Conference
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• 1999.08a
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• pp.83-90
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• 1999

Electron crystallography of two-dimensional crystalsand electron cryo-microscopy is becoming an established method for determining the structure and function of a variety of membrane proteins that are providing difficult to crystallize in three dimension. In this study this technique has been used to investigate the structure of a ~160 kDa reaction centre sub-core complex of photosystem II. Photosystem II is a photosynthetic membrane protein consisting of more than 25 subunits. It uses solar energy to split water releasing molecular oxygen into the atmosphere and creates electrochemical potential across the thylakoid membrane, which is eventually utilized to generate ATP and NADPH. Images were taken using Philips CM200 field emission gun electron microscope with an acceleration voltage of 200kW at liquid nitrogen temperature. In total, 79 images recorded dat tilt angles ranging from 0 to 67 degree yielded amplitudes and phases for a three-dimensional map with an in-plant resolution of 6$\AA$ and 11.4$\AA$ in the third dimension shows at least 23 transmembrane helices resolved in a monomeric complex, of which 18 were able to be assigned to the D1, D2, CP47 , and cytochrome b559 alfa beta-subunits with their associated pigments that ae active in electron transport (Rhee, 1998, Ph.D.thesis). The D1/D2 heterodimer is located in the central position within the complex and its helical scalffold is remarkably similar to that of the reaction centres not only in purple bacteria but also in plant photosystem I (PSI) , indicating a common evoluationary origin of all types of reaction centre in photosynthetic organism known today 9RHee et al. 1998). The structural homology is now extended to the inner antenna subunit, ascribed to CP47 in our map, where the 6 transmembrane helices show a striking structural similarity to the corresponding helices of the PSI reaction centre proteins. The overall arrangement of the chlorophylls in the D1 /D2 heterodimer, and in particular the distance between the central pair, is ocnsistent with the weak exciton coupling of P680 that distinguishes this reaction centre from bacterial counterpart. The map in most progress towards high resolution structure will be presented and discussed.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.25 no.3
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• pp.237-243
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• 2012

The thickness optimization of the gearbox housing for 5MW wind turbine is carried out with the help of the efficient structure analysis model and the approximation model of objective function. Wind turbine gearbox is a complex structural system composed of a number of gear trains, shafts, bearing and gearbox housing, requiring a tremendous number of elements for the structural analysis and design. In this paper, an effective analysis and design model considering the tooth stiffness of helical gears is proposed. It enables to significantly reduce the total element number and the analysis time. Through the numerical optimization of housing thickness making use of the effective gearbox model and the approximate model of objective function, the total weight of the gearbox housing is minimized. It has been observed from the numerical experiment that the approximation model is reliable and the optimization result is acceptable and verified analysis.

• Proceedings of the Korean Vacuum Society Conference
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• 2013.02a
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• pp.550-551
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• 2013

Large-area RF-driven ion source is being developed at Germany for the heating and current drive of ITER plasmas. Negative hydrogen (deuterium) ion sources are major components of neutral beam injection systems in future large-scale fusion experiments such as ITER and DEMO. RF ion sources for the production of positive hydrogen ions have been successfully developed at IPP (Max-Planck- Institute for Plasma Physics, Garching) for ASDEX-U and W7-AS neutral beam injection (NBI) systems. In recent, the first NBI system (NBI-1) has been developed successfully for the KSTAR. The first and second long-pulse ion sources (LPIS-1 and LPIS-2) of NBI-1 system consist of a magnetic bucket plasma generator with multi-pole cusp fields, filament heating structure, and a set of tetrode accelerators with circular apertures. There is a development plan of large-area RF ion source at KAERI to extract the positive ions, which can be used for the second NBI (NBI-2) system of KSTAR, and to extract the negative ions for future fusion devices such as ITER and K-DEMO. The large-area RF ion source consists of a driver region, including a helical antenna (6-turn copper tube with an outer diameter of 6 mm) and a discharge chamber (ceramic and/or quartz tubes with an inner diameter of 200 mm, a height of 150 mm, and a thickness of 8 mm), and an expansion region (magnetic bucket of prototype LPIS in the KAERI). RF power can be transferred up to 10 kW with a fixed frequency of 2 MHz through a matching circuit (auto- and manual-matching apparatus). Argon gas is commonly injected to the initial ignition of RF plasma discharge, and then hydrogen gas instead of argon gas is finally injected for the RF plasma sustainment. The uniformities of plasma density and electron temperature at the lowest area of expansion region (a distance of 300 mm from the driver region) are measured by using two electrostatic probes in the directions of short- and long-dimension of expansion region.

• Proceedings of the Korean Biophysical Society Conference
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• 2003.06a
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• pp.75-75
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• 2003

We report here the results on N-acetyl-N'-dimethylamide of proline (Ac-Pro-NM $e_2$) calculated using the ab initio molecular orbital method with the self-consistent reaction field (SCRF) theory at the HF level with the 6-31+G(d) basis set to investigate the conformational preference of polyproline depending on the cis/trans peptide bonds and down/up puckerings along the backbone torsion angle $\square$ in the gas phase, chloroform, and water. In the gas phase, Ac-Pro-NM $e_2$ has seven local minima of tFd, tFu, cFd, cFu, cAu, tAu, and cAd conformations. In particular, polyproline conformations tFd, tFu, cFd, and cFu are found to be more stable than $\square$-helical conformations cAu, tAu, and cAd. In contrast, Ac-Pro-NHMe has seven local minima of tCd, tCu, cBd, cAu, tAu, cFd, and cFu conformations. Conformations tCd and tCu are found to be most stable, which is ascribed to the intramolecular hydrogen bond between C=O of acetyl group and $N^{~}$ H of N'-methyl amide group. The stability of the cFd conformation (i.e., the polyproline I structure) in chloroform is somewhat increased, relative to that in water, although tFd and tFu conformations (i.e., the polyproline II structure) are dominate both in chloroform and water. The population of backbone conformations feasible in chloroform and water is consistent with the experiments. This work is supported by a Korea Research Foundation Grant (KRF-2002-041-C00129).

• Proceedings of the Korean Biophysical Society Conference
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• 2003.06a
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• pp.68-68
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• 2003

HP(2-20) (AKKVFKRLEKLEKLFSKIQNDK) derived from the N-terminus of Helicobacter pylori Ribosomal Protein L1 shows potent antimicrobial activity against bacterial, fungi and cancer cells without cytotoxic effect. In order to investigate the relationships between antimicrobial activity and the structures, several analogues have been designed and synthesized. The structures of these peptides in SDS micelles have been investigated using NMR spectroscopy and they revealed that analogue 3 has the longest, well-defined alpha-helix from Val5 to Trp19. NOESY experiments performed on HP and its analogues in nondeuterated SDS micelles show that protons in the indole ring of Trp16 are in close contact with methylene protons of SDS micelles. In order to probe the position of HP and its analogues relative to the SDS micelles, spin-labeled stearate was added. Large effects are observed for the chemical shifts and the intensities of Phe5, Glu9, Phe12, and Trp16 within the helix region by 16-doxylstearate. This result implies that 16-doxylstearate is located in the center of the micelles and the hydrophobic phase of the amphiphilic ${\alpha}$-helix is located in contact with the acyl chains of the micelles. Also, Lys3 and Lys4 at N-terminus and Lys20 at C-terminus may produce an optimal arrangement for electrostatic interactions between the sulfate head groups of the SDS and the positively charged lysyl N$\sub$3/$\^$+/. Interactions between the indole ring of Trp and the membrane, as well as the amphiphilic ${\alpha}$-helical structure of HP induced by Trp at the C-terminus may allow HP to span the lipid bilayer. These structural features are crucial for their potent antibiotic activities.

• Proceedings of the Korean Biophysical Society Conference
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• 2003.06a
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• pp.69-69
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• 2003

Human CD99 is a ubiquitous 32-kDa transmembrane protein encoded by the mic2 gene. The major cellular functions of CD99 protein are related to homotypic cell adhension, apoptosis, vesicular protein transport, and differentiation of thymocytes or T cells. Recently it has been reported that expression of a splice variant of CD99 transmembrane protein (Type I and Type II) increases invasive ability of human breast cancer cells. To understand structural basis for cellular functions of CD99 (Type I), we have initiated studies on hCD99$^{TMcytoI}$ and hCD99$^{cytoI}$ using circular dichroism (CD) and multi-dimensional NMR spectroscopy. CD spectrum of hCD99$^{TMcytoI}$ in the presence of 200mM DPC and CHAPS displayed an existence $\alpha$-helical conformation. The solution structure of hCD99$^{cytoI}$ determined by NMR is composed of one N-terminal $\alpha$-helix, $\alpha$A, two C-terminal short $\alpha$-helix segments, $\alpha$B and $\alpha$C. While $\alpha$A and $\alpha$B are connected by the long flexible loop, $\alpha$B and $\alpha$C connected by type III$\beta$-turn. Although it has been rarely figured out the correlation between structure and functional mechanism of hCD99$^{TMcytoI}$ and hCD99$^{cytoI}$, there is possibility of dimerization or oligomerization. In addition, the feasible mechanism of hCD99$^{cytoI}$ is that it could have intramolecular interaction between the N- and C- terminal domain through large flexible AB loop.

• Composites Research
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• v.33 no.3
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• pp.169-175
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• 2020

The compression and tension test were performed to evaluate the stiffnesses of the conical composite lattice structures and results of test were compared with finite element analysis results. Because of difficulty to perform simple tension and compression test due to conical shape, suitable specimens and jig for test were made. Subelements extracted from the structure were prepared for compression test. Compression test of subelement was performed and compressive strains in fiber direction were measured. Compressive stiffness of the helical rib was verified by finite element analysis results. For stiffness of hoop rib, hoop ring specimens were extracted from the structure. Tension test of hoop ring specimen was performed to apply bending deformation to hoop rib. Stiffness of hoop rib was verified by finite element model considering various fiber volume fraction in thickness direction.

• 한국지구과학회:학술대회논문집
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• 2010.04a
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• pp.53-53
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• 2010

지표위의 어떤 지점에서의 지구자기의 수평분력 방향과 진북방향 사이의 각을 편각(Declination)이라고 정의한다. 쉽게 말하면 편각은 나침반의 자침이 가러 키는 방향과 진북방향과의 사이 각을 말한다. 대부분의 사람들은 나침반의 자침이 북자기극(North magnetic pole)을 가러킨다고 잘못알고 있다. 지구 다이나모설(Geodynamo theory)에 의하면 주로 철(약 90%)로 구성된 외핵 속에서 계속 생성 유지되고 있는 복잡한 (각각 나선형(helical)의 회전축에 대체로 평행하거나 평행하지 않은) 대류(Convection currents)에 수반하는 전류가 복잡한 지구자기장을 형성한다. 지표상에서 측정한 지구자기장의 자료를 Spherical harmonic analysis 으로 분석하면 한 개의 커다란 쌍극자(Dipole) (Inclined geocentric dipole 또는 주된 자기장(Main field) 이라고 부름), 적도쌍극자(Equatorial dipole), 4극자 (Quadrupoles), 8극자(Octupoles) 등의 여러 개의 크고 작은 쌍극자들의 총합이 지구자기장의 근원인 것처럼 해석되고 있다. 어떤 지점에서의 지구자기장의 방향은 외핵에서 생성된 천체 자기장에서 Main field를 제거한 나머지 자기장과, 상부 맨틀(upper mantle), 지각 및 지표상에 존재하는 인공 물체 또는 암석 및 광석 등의 잔류자기 및 유도자기 그리고 지형 등의 영향으로 결정된다. 어떤 지점에서의 지구자기장의 방향은 태양풍(Solar wind)과 전리층 사이의 상호작용 등의 외부자장(external field)의 영향도 받는다. 비쌍극자 자장(Non-dipole field)은 지표상에서 측정되는 총자기장에서 외핵에서 생성된 주된 자기장(Main field) 즉, 지구의 회전축에서 약 11.5도 기울어진 쌍극자 자장을 제거하고 남는 자기장을 말한다. 따라서 편각은 비쌍극자자장의 영향을 가장 많이 받는다. 비쌍극자 자장은 정지한 상태의 자장(standing field) 과 매년 서쪽으로 약 0.2도 움직이는 Westward drift하는 자장으로 크게 두 가지로 구분된다. 쌍극자 자장의 방향은 매우 느리게 변하지만 그 세기는 현재 비교적으로 빠르게 약해지고 있다. 비교적으로 매우 빠르게 변하는 비쌍극자 자장의 변화를 영년변화(Secular variation) 이라고 한다.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.18 no.12
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• pp.2823-2830
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• 2014

The antenna miniaturization technique involves the increment of the electrical length of the resonator the variation of the physical appearance of the antenna. The most typical method of size reduction is designing helical antenna, meander antenna, and fractal antenna. Size reduction using fractal antenna is proposed in this paper. Fractal koch curve has been etched in microstrip patch antenna to downsize the antenna at ISM (Industrial Scientific and Medical) frequency band of 2.45 GHz koch curve microstrip patch antenna ha FR4 epoxy substrate with dielectric constant 4.7, loss tangent equal to 0.02 and dielectric high of 1.6 mm. The designed antenna is fabricated using etching process. The fabricated antenna has return loss of 2.45 GHz, VSWR of 1.1492, and impedance is matched to $46{\Omega}$.

• Bulletin of the Korean Chemical Society
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• v.24 no.10
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• pp.1478-1484
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• 2003

CRAMP was identified from a cDNA clone derived from a mouse femoral marrow cells as a member of cathelicidin-derived antimicrobial peptide. Tertiary structure of CRAMP in TFE/$H_2O$ (1 : 1, v/v) solution has been determined by NMR spectroscopy previously and consists of two amphipathic $\alpha-helices$ from Leu4 to Lys10 and from Gly16 to Leu33. These two helices are connected by a flexible region from Gly11 to Gly16. Analysis of series of fragments composed of various portion of CRAMP revealed that an 18-residue fragment with the sequence from Gly16 to Leu33 (CRAMP-18) was found to retain antibacterial activity without cytotoxicity. The effects of two Phe residues at positions 14 and 15 of CRAMP-18 on structure, antibacterial activity, and interaction with lipid membranes were investigated by $Phe^{14,15}$ ${\rightarrow}$ Ala substitution (CRAMP-18-A) in the present study. Substitution of Phe with Ala in CRAMP-18 caused a significant reduction on antibacterial and membrane-disrupting activities. Tertiary structures of CRAMP-18 in 50% TFE/$H_2O$ (1 : 1, v : v) solution shows amphipathic ${\alpha}$-helix, from $Glu^2{\;}to{\;}Leu^{18}$, while CRAMP-18-A has relatively short amphipathic ${\alpha}$-helix from $Leu^4{\;}to{\;}Ala^{15}$. These results suggest that the hydrophobic property of $Phe^{14}{\;}and{\;}Phe^15$ in CRAMP-18 is essential for its antibacterial activity, ${\alpha}$-helical structure, and interactions with phospholipid membranes.