• Journal of the Society of Cosmetic Scientists of Korea
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• v.31 no.2 s.51
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• pp.197-206
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• 2005

In the present study, the Chuncheon buckwheat extracts prepared from its seed coats, seeds and stems were used to determine anti-oxidative effects, the content of rutin and phytic acid, and the protective role against cadmium at the cellular level. futhermore, it was evaluated whether the buckwheat, mainly known as a healthy food source, might be applicable to functional cosmetics. Up to $100 {\mu}g/mL$ of the extract was not toxic in HaCaT and B16F10 cell lines using MTT assay. The anti-oxidative capacity of superoxide radicals was shown in seed coats extracts > stem extracts=seed extracts. Although its content of rutin, known as one of effective anti-oxidants, mainly exists in the stem, any extract did not eliminate hydroxyl radicals. Phytic acid, known as a heavy metal-chelate agent, was highly concentrated in the stem. The Chuncheon buckwheat extract had $10\%$ protective effect against the treatment of $50{\mu}M$ cadmium at which $50\%$ of HaCaT cells survived. Confocal laser scanning microscope revealed the intracellular generation of reactive oxygen species (ROS) by cadmium treatment. Finally, we identified that the stem extract had the most protective effect on the elimination of ROS.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.10a
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• pp.39-39
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• 2019

섬초롱꽃은 울릉군에 한정적으로 분포하는 여러해살이풀이다. 산림청에서는 약관심종(LC) 등급의 희귀식물이자 특산식물로 분류하고 있으며, 꽃의 관상가치가 특히 높아 화단용 소재, 절화용, 화분재배 등으로 흔히 이용되고 있다. 따라서 본 연구는 섬초롱꽃의 대량증식 시, 중금속이 미칠 수 있는 영향에 대해 파악하고자 수행하였다. 실험에 사용한 중금속은 카드뮴(Cd), 철(Fe) 두 종류이며, 두 종류 모두 1% 한천(agar)배지에 10, 25, 50, 100, 200 mg/L 농도로 녹여 사용하였다. 발아율 및 유근 생장을 위해 종자 및 유묘를 치상 후, $25^{\circ}C$ 항온 생장상에서 발아율 및 유근 발달을 관찰하였다. 발아율 측정결과, 아무처리도하지 않은 1% 한천(agar) 배지에서 $62.7{\pm}7.4%$로 가장 높았고 중금속 농도가 높아질수록 감소하였다. 카드뮴(Cd) 처리구에서는 10, 25, 50, 100, 200 mg/L 농도에서 각각 $17.3{\pm}1.3$, $2.7{\pm}1.3$, $1.3{\pm}1.3$, 0, 0%로 나타났다. 한편, 철(Fe) 처리구에서는 10, 25, 50, 100, 200 mg/L 농도에서 각각 $46.7{\pm}4.8$, $24.0{\pm}2.3$, $36.0{\pm}6.9$, $20.0{\pm}2.3$, 0%로 나타났다. 유근의 평균 생장 길이(mm)는 아무처리도 하지 않은 1% 한천(agar) 배지에서 19.6 mm로 가장 높았고, 중금속 농도가 높아질수록 낮게 나타났다. 카드뮴(Cd) 처리구에서는 10, 25, 50, 100, 200 mg/L 농도에서 각각 4.6 mm, 1.9 mm, 0.7 mm, 0.9 mm, 0 mm 생장한 것으로 나타났다. 한편, 철(Fe) 처리구에서는 10, 25, 50, 100, 200 mg/L 농도에서 각각 15.9 mm, 8.6 mm, 8.1 mm, 0.9 mm, 0 mm 생장한 것으로 나타났다. 실험결과를 종합해 볼 때, 섬초롱꽃 종자 발아 및 유근 생장의 억제는 카드뮴(Cd) 처리구에서 높게 나타났고, 철(Fe) 처리구에서는 비교적 영향을 덜 미치는 것으로 나타났다. 특히 카드뮴은 10 mg/L 농도만 처리하더라도 약 3.6~4.2배의 발아율 생장율 감소가 나타나, 섬초롱꽃 대량증식 시, 치명적일 것으로 판단된다. 본 실험의 결과는 섬초롱꽃 종자를 이용한 대량증식 및 유묘재배에 있어, 토양을 선택하는데 중요한 기초자료로서 활용될 것으로 기대된다.

• Clean Technology
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• v.26 no.4
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• pp.263-269
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• 2020

Zeolite was synthesized hydrothermally using the water-treatment sludge, and the effects of various synthesis parameters like reaction temperature, reaction time, and Na2O/SiO2 molar ratio on the crystallization of zeolite were investigated. Crystal structure, physical property, and thermal stability of zeolite crystals were characterized by X-ray powder diffraction, FTIR spectroscopy, BET nitrogen adsorption, and TGA measurements. The removal efficiencies of nitrogen in ammonia, heavy metal ions, and TOC were calculated to evaluate zeolite's adsorption capacity. The primary chemical composition of water-treatment sludge was 28.79% Al2O3 and 27.06% SiO2. The zeolites were synthesized by merely employing the water-treatment sludge as silica and alumina sources without additional chemicals. Zeolite crystals synthesized through the water-treatment sludge were confirmed as an A-type zeolite structure. Zeolite A had the highest crystallinity obtained from a gel with the molar composition 2.1Na2O-Al2O3-1.6SiO2-65H2O after 5 h at a temperature of 90 ℃. The specific surface area of zeolite obtained was 55 ㎡ g-1, which was higher than commercial zeolite A. The removal efficiency of nitrogen in ammonia was 68% after 3 h of reaction time, while the removal efficiencies of Pb2+ and Cd2+ ions were 99.1% and 99.3%, respectively. These results indicate active ion exchange between Pb2+ or Cd2+ ion and Na+ ion in the zeolite framework. The adsorption experiments on the different zeolite addition conditions were performed for 3 h with 300 ppm humic acid. Based on the results, TOC's highest efficiency was 83% when 5 g of zeolite was added.

• Economic and Environmental Geology
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• v.45 no.3
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• pp.217-235
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• 2012

We evaluated the geochemical characteristics and their potential pollution of Asian Dusts in Daejeon, Korea during spring 2007. Compared with the chemical compositions of soils in source area of Asian Dust, those of aerosols in Daejeon were enriched with trace elements (ten to hundred fold), inferring that pollutants from China have affected on local environment in adjoining country such as Korea. Chemical analysis of aerosols during Asian dust showed that fine particles ($PM_{2.5}$) contained high contents of trace elements such as Cr, Cu, Pb, Zn, V, S, As, Cd, Co, Ni, Mo, Sb, Cs, Rb, Th, Sc and Y. In the case of TSP (Total Suspended Particle), Zr, Sr, Ba, Li, Th and U were contained much more than other trace elements. The contents of some elements (i.e. Li, Cs, Co, U, Cr, Ni, Rb, V, Th, Y, Sr and Sc) in aerosols collected in Asian Dust period, which are not likely enriched by air pollutants, were higher (2 - 4.2 fold) than those in Non Asian Dust period, indicating that these elements could be used as indicator elements for determining the occurrence of Asian Dust phenomena (especially, Sr, V, Cr & Li). In the case of Asian Dust coming through the big cities and/or industrial areas of China, the domestic aerosols had higher contents of trace elements (such as S, Cd, Zn, Pb, Cu, Mo and As) than those from Northeastern China via North Korea, indicating that the transportation courses of air mass are very important to determine the pollution degrees. Using the enrichment factors of trace elements in aerosols during Asian Dust and Non Asian Dust, we identified that some elements (i.e. S, Zn, Cu, Pb, As, Mo and Cd) were most problematic in terms of environmental hazard aspects, and these elements could affect adverse effects on human health as well as ecosystem and surface environment (soil and water) through long-lived precipitation.

• Journal of Korean Society of Forest Science
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• v.86 no.3
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• pp.324-333
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• 1997

The research has been made for the effects of the pollution by the abandoned coal mine drainage on the physical and chemical properties of soil, stream sediment and soil water. The soils overspreaded by the abandoned coal don't develop solum and the bulk density is $1.83g/m^3$, compared with $1.14-1.38g/m^3$ in the other forest soils. The soil pH range in coal bearing region ie, from 4.01 to 4.11 and non-coal bearing soil range is from 5.03 to 5.13. Heavy metals such as As, Cr, Ni, Mo and Ba of coal bearing soils and polluted stream sediments have larger concentration than those of non-coal content and non-polluted. Especially As and Mo concentrations are largely high in coal bearing. The relative ratios $K_2O/Na_2O$ of geochemical elements are higher in coal bearing soil and polluted stream sediments than those of non-coal bearing soils and non-polluted stream sediments as well as black shales of the Changri Formation. However, $MgO+Fe_2O_3+TiO_2/CaO+K_2O$ are the opposite trends, so that the ratios are lower in the polluted regions. The soil water pHs in the polluted regions are the strong acid(pH3.4-4.2) and buffer capacity of the polluted soil is low because canons such as $Na^+$, $K^+$, $Mg^{+2}$are leached by the acidification.

• Korean Journal of Soil Science and Fertilizer
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• v.44 no.6
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• pp.1306-1313
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• 2011

A liquid fertilizer treated with slurry composting and biofiltration (SCB) process has been applied increasingly on agricultural field but the effects on the soil properties and crop production has not been throughly evaluated. This study was conducted to investigate the effect of the SCB application on soil chemical properties and the growth of radish and corn. SCB liquid fertilizer as a basal fertilization was treated with five levels based on $6kg\;10a^{-1}$ for radish and $10kg\;10a^{-1}$ for corn. The experimental design was the completely randomized block design with five levels and three replicates. Electrical conductivity (EC), $NO_3$-N, Exch. K and Exch. Na increased depending on the treatment levels of SCB. There were no changes in soil organic matter, Avail. $P_2O_5$, Exch. Ca and Exch. Mg. EC, $NO_3$-N and Exch. Na content decreased as precipitation increased. Especially, they decreased up to the initial condition before the treatment after the heavy rainy season in 2008. Although Exch. K decreased at the rainy season, they remained relatively higher content after the experiment on August, 2008. Fresh weight and the amount of N uptake of radish increased due to the levels of SCB, but corn did not present any significant increase. It is recommended that we need to decide the proper amount of SCB as well as the application method on the field to increase the productivity and decrease environmental stress. Additional experiments also need to clarify the effect of the trace element and heavy metal accumulations due to long term application of SCB.

• Journal of the Korean Society of Marine Environment & Safety
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• v.28 no.2
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• pp.212-223
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• 2022

For the remediation and restoration of contaminated sediment at the West Sea-Byeong dumping site, dredged materials was dumped in 2013, 2014, 2016, and 2017. The physicochemical properties and benthic fauna in surface sediments of the capping area (5 stations) and natural recovery area (2 stations) were analyzed annually from 2014 to 2020 to evaluate the capping effect of the dredged materials. The natural recovery area had a finer sediment with a mean particle size of 5.91-7.64 Φ, while the sediment in the capping area consisted of coarse-grained particles with a mean particle size of 1.47-3.01 Φ owing to the capping effect of dredged materials. Considering that the contents of organic matters (COD, TOC, and TN) and heavy metals in the capping area are approximately 50 % lower (p<0.05) than that in the natural recovery area, it is judged that there is a capping effect of dredged materials. As a result of analyzing macrobenthic assemblages, the number of species and ecological indices of the capping area were significantly lower than that of the natural recovery area (p<0.05). The number of species and ecological indices at the capping area were increased for the first four years after the capping in 2013 and 2014 and then tended to decrease thereafter. It is presumed that opportunistic species, which have rapid growth and short lifetime, appeared dominantly during the initial phase of capping, and the additory capping in 2016 and 2017 caused re-disturbance in the habitat environment. In the natural recovery and capping areas, Azti's Marine Biotic Index (AMBI) was evaluated as a fine healthy status because it maintained the level of 2^nd grades (Good), whereas Benthic Pollution Index (BPI) remained at the 1^st and 2^nd grade. Therefore, capping of dredged materials for remediation of contaminated sediment in the dumping site has the effect of reducing the pollution level. However, in terms of the benthic ecosystem, it is recommended that the recovery trend should be monitored long-term. Additionally, it is necessary to introduce an adaptive management strategy when expanding the project to remediate the contaminated sediment at the dumping area in the future.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.13 no.2
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• pp.121-128
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• 2008

As an aquatic ecotoxicity test method, a bioassay using the inhibition of sporualtion of the green macroalga, Ulva pertusa, has been developed. Optimal test conditions determined for photon irradiance, pH, salinity and temperature were $100\;{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, $7{\sim}9$, $25{\sim}35\;psu$ and $15{\sim}20^{\circ}C$, respectively. The validity of the test endpoint was evaluated by assessing the toxicity of four metals (Cd, Cu, Pb, Zn) and elutriates of sewage or waste sludge collected from 9 different locations. When the metals were assayed, the $EC_{50}$ values indicated the following toxicity rankings: Cu ($0.062\;mg{\cdot}L^{-1}$) > Cd ($0.208\;mg{\cdot}L^{-1}$) > Pb ($0.718\;mg{\cdot}L^{-1}$) > Zn ($0.776\;mg{\cdot}L^{-1}$). When compared with other commonly used bioassays of metal pollution listed on US ECOTOX database, the sporualtion test proved to be the most sensitive. Ulva sporulation was significantly inhibited in all elutriates with the greatest and least effects observed in elutriates of sludge from industrial waste ($EC_{50}=6.78%$) and filtration bed ($EC_{50}=15.0%$), respectively. The results of the Spearman rank correlation analysis for $EC_{50}$ data versus the concentrations of toxicants in the sludge presented a significant correlation between toxicity and four heavy metals(Cd, Cu, Pb, Zn). The method described here is sensitive to toxicants, simple to use, easy to interpret and economical. It is also easy to procure samples and maintain cultures. The present method would therefore probably make a useful assessment of aquatic toxicity of a wide range of toxicants. In addition, the genus Ulva has a wide geographical distribution and species have similar reproductive processes, so the test method would have a potential application worldwide.

• Journal of Sericultural and Entomological Science
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• v.13 no.1
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• pp.49-59
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• 1971

This experiment was conducted, how making use of "Seracol 100", a kind of nonionic surface active reagent, during cocoon cooking and silk reeling process which is mainly used in Korea and developed by one of the authors since 1965. Main purpose of the experiment is to investigate the influence of the activity of the reelability aid reagent on various degree of hardness component and various salts which are contained in filature water being used by silk factories now. Specifically, it was tried to determine the effects of the reelability aid reagent added to each sample water of artificially differentiated the degree of hardness component upon sericin solubility. Some of the major findings are summarized as follows. 1. "Seracol 100", with below 5$^{\circ}$dH water, increased sericin solubility in each sample water, but above $10^{\circ}$dH the effect of it shows a significant relationship with the different degree of hardness component. Besides the component (MgCO$_3$)$_4$ㆍMg(OH)$_2$, generally, the higher the degree of hardness in the water, the less the amount of desolved sericin in the water showed. There is little or no difference between 1000 times diluted "Seracol 100" water and 2000 times diluted "Seracol 100" water in terms of sericin solubility. 2. The different kind of degree of hardness component shows a significant relationship with sericin solubility, In case use of "Seracol 100", Mg hardness component affected on the sericin solubility more than Ca hardness. But in the control, contrary to this, that is Ca hardness component did more than Mg hardness, 3. The different kind of salts in degree of hardness component show a significant relationship with sericin solubility. In the control water, nitrate is the greatest among salts affecting on sericin solubility, next chloride and sulfate are in order. In case of "Seracol 100" water, chloride is the least among salts, below $10^{\circ}$dH, next sulfate and nitrate are in order, and above 15$^{\circ}$dH, next nitrate and sulfate are in order. 4. In case of "Seracol 100" water, the more contained heavy metal salts (Fe, Al, Cu, Mn) in the water, the less sericin solubility showed. It is found that there is little or no difference among other salts in terms of sericin solubility. But alkaline metal salt remarkably increased sericin solubility. 5. In case of "Seracol 100" water, tinting of the water was affected by Ca salts more than by Mg salts. Among other salts, only Al and Fe affected on the tinting of the water, specifically, in view of the fact that "Seracol 100" water increase the tinting of Fe salt water, but decrease the tinting of raw silk. It is thought that "Seracol 100" deter Fe$^{+2}$ from absorbing to raw silk by deteriorating the activity of Fe$^{+2}$ . 6. "Seracol 100" have the effect on osmosis of the water, After treatment until 2hrs the osmosis of 1000 times diluted "Seracol 100" water is the greatest, next orders are 2000 times diluted "Seracol 100" and control. From 2 hrs to 6 hrs the osmosis of 2000 times diluted water is the greatest, next orders are 1000 times diluted water and control. After 6 hrs the osmosis of the water is the same order as above. 7. In view of tile fact that "Seracol 100" have the effect to control the degree of hardness during the treatment of cocoon layer in the water, it is thought that, in varying degree of hardness in the water, there is a significant relationship between "Seracol 100" and degree of hardness components in cocoon layer.

• Journal of the Korean Society of Radiology
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• v.15 no.7
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• pp.965-973
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• 2021

Since radiation therapy is irradiated with high-energy X-rays in a variety of at least 20 Gy to 80 Gy, a high dose is administered to the local area where the tumor is located, and various side effects of some normal tissues are expected. Currently, in clinical practice, lead, a representative material, is used as an effort to shield normal tissues, but lead is classified as a heavy metal harmful to the human body, and a large amount of skin contact can cause poisoning. Therefore, this study intends to manufacture a measurement sheet that can compensate for the limitations of lead using the materials Tungsten, Brass, and Copper of the 3D printer of the FDM (Fused Deposition Modeling) method and to investigate the penetration performance. Tungsten mixed filament transmission measurement sheet size was 70 × 70 mm and thickness 1, 2, 4 mm using a 3D printer, and a linear accelerator (TrueBeam STx, S/N: 1187) was measured by irradiating 100 MU at SSD 100 cm and 5 cm in water using a water phantom, an ion chamber (FC-65G), and an elcetrometer (PTW UNIDOSE), and the permeability was evaluated. As a result of increasing the measurement sheet of each material by 1 mm, in the case of Tungsten sheet at 3.8 to 3.9 cm in 6 MV, the thickness of the lead shielding body was thinner than 6.5 cm, and in case of Tungsten sheet at 4.5 to 4.6 cm in 15 MV. The sheet was thinner than the existing lead shielding body thickness of 7 cm, and equivalent performance was confirmed. Through this study, the transmittance measurement sheet produced using Tungsten alloy filaments confirmed the possibility of transmission shielding in the high energy region. It has been confirmed that the usability as a substitute is also excellent. It is thought that it can be provided as basic data for the production of shielding agents with 3D printing technology in the future.