• Asian-Australasian Journal of Animal Sciences
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• v.31 no.8
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• pp.1373-1380
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• 2018

Objective: This study was designed to investigate the effects of temperature and storage time on the evolution of bacterial communities in swine manure. Methods: Manure was stored at $-20^{\circ}C$, $4^{\circ}C$, $20^{\circ}C$, or $37^{\circ}C$ and sampled at 7-day intervals over 28 days of storage, for a total of 5 time points. To assess the bacterial species present, 16S ribosomal RNA gene sequences were analyzed using pyrosequencing. Results: After normalization, 113,934 sequence reads were obtained, with an average length of $466.6{\pm}4.4bp$. The diversity indices of the communities reduced as temperature and storage time increased, and the slopes of rarefaction curves decreased from the second week in samples stored at $-20^{\circ}C$ and $4^{\circ}C$. These results indicate that the richness of the bacterial community in the manure reduced as temperature and storage time increased. Firmicutes were the dominant phylum in all samples examined, ranging from 89.3% to 98.8% of total reads, followed by Actinobacteria, which accounted for 0.6% to 7.9%. A change in community composition was observed in samples stored at $37^{\circ}C$ during the first 7 days, indicating that temperature plays an important role in determining the microbiota of swine manure. Clostridium, Turicibacter, Streptococcus, and Lactobacillus within Firmicutes, and Corynebacterium within Actinobacteria were the most dominant genera in fresh manure and all stored samples. Conclusion: Based on our findings, we propose Clostridium as an indicator genus of swine manure decomposition in an anaerobic environment. The proportions of dominant genera changed in samples stored at $20^{\circ}C$ and $37^{\circ}C$ during the fourth week. Based on these results, it was concluded that the microbial communities of swine manure change rapidly as storage time and temperature increase.

• Mycobiology
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• v.45 no.4
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• pp.392-400
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• 2017

Peanut yield and quality are seriously affected by pod rot pathogens worldwide, especially in China in recent years. The goals of this study are to analyze the structure of fungal communities of peanut pod rot in soil in three peanut cultivars and the correlation of pod rot with environmental variables using 454 pyrosequencing. A total of 46,723 internal transcribed spacer high-quality sequences were obtained and grouped into 1,706 operational taxonomic units at the 97% similarity cut-off level. The coverage, rank abundance, and the Chao 1 and Shannon diversity indices of the operational taxonomic units were analyzed. Members of the phylum Ascomycota were dominant, such as Fusarium, Chaetomium, Alternaria, and Sordariomycetes, followed by Basidiomycota. The results of the heatmap and redundancy analysis revealed significant variation in the composition of the fungal community among the three cultivar samples. The environmental conditions in different peanut cultivars may also influence on the structure of the fungal community. The results of this study suggest that the causal agent of peanut pod rot may be more complex, and cultivars and environmental conditions are both important contributors to the community structure of peanut pod rot fungi.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.141-141
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• 2017

The ubiquitin-proteasome pathway is the major regulatory mechanism in a number of cellular processes for selective degradation of proteins and involves three steps: (1) ATP dependent activation of ubiquitin by E1 enzyme, (2) transfer of activated ubiquitin to E2 and (3) transfer of ubiquitin to the protein to be degraded by E3 complex. F-box proteins are subunit of SCF complex and involved in specificity for a target substrate to be degraded. F-box proteins regulate many important biological processes such as embryogenesis, floral development, plant growth and development, biotic and abiotic stress, hormonal responses and senescence. However, little is known about the F-box genes in wheat. The draft genome sequence of wheat (IWGSC Reference Sequence v1.0 assembly) used to analysis a genome-wide survey of the F-box gene family in wheat. The Hidden Markov Model (HMM) profiles of F-box (PF00646), F-box-like (PF12937), F-box-like 2 (PF13013), FBA (PF04300), FBA_1 (PF07734), FBA_2 (PF07735), FBA_3 (PF08268) and FBD (PF08387) domains were downloaded from Pfam database were searched against IWGSC Reference Sequence v1.0 assembly. RNA-seq paired-end libraries from different stages of wheat, such as stages of seedling, tillering, booting, day after flowering (DAF) 1, DAF 10, DAF 20, and DAF 30 were conducted and sequenced by Illumina HiSeq2000 for expression analysis of F-box protein genes. Basic analysis including Hisat, HTseq, DEseq, gene ontology analysis and KEGG mapping were conducted for differentially expressed gene analysis and their annotation mappings of DEGs from various stages. About 950 F-box domain proteins identified by Pfam were mapped to wheat reference genome sequence by blastX (e-value < 0.05). Among them, more than 140 putative F-box protein genes were selected by fold changes cut-offs of > 2, significance p-value < 0.01, and FDR<0.01. Expression profiling of selected F-box protein genes were shown by heatmap analysis, and average linkage and squared Euclidean distance of putative 144 F-box protein genes by expression patterns were calculated for clustering analysis. This work may provide valuable and basic information for further investigation of protein degradation mechanism by ubiquitin proteasome system using F-box proteins during wheat development stages.

• Journal of Nutrition and Health
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• v.51 no.4
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• pp.275-286
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• 2018

Purpose: Our previous study demonstrated that persimmon (Diospyros kaki Thumb.) at different stages of ripening provided different protective effects against high-fat/cholesterol diet (HFD)-induced dyslipidemia in rats. In this study, we compared the metabolites profile and gene expressions related to triglyceride (TG)/cholesterol metabolism in vitro and in vivo after treating with persimmon water extracts (PWE) or tannin-enriched persimmon concentrate (TEP). Methods: Primary and secondary metabolites in test materials were determined by GC-TOF/MS, UHPLC-LTQ-ESI-IT-MS/MS, and UPLC-Q-TOF-MS. The expression of genes related to TG and cholesterol metabolism were determined by RT-PCR both in HepG2 cells stimulated by oleic acid/palmitic acid and in liver tissues obtained from Wistar rats fed with HFD and PWE at 0, 150, 300, and 600 mg/d (experiment I) or TEP at 0, 7, 14, and 28 mg/d (experiment II) by oral gavage for 9 weeks. Results: PLS-DA analysis and heatmap analysis demonstrated significantly differential profiling of metabolites of PWE and TEP according to processing of persimmon powder. In vitro, TEP showed similar hypolipidemic effects as PWE, but significantly enhanced hypocholesterolemic effects compared to PWE in sterol regulatory element-binding protein 2 (SREBP2), HMG-CoA reductase (HMGCR), proprotein convertase subtilisin/kexin type 9 (PCSK9), cholesterol $7{\alpha}-hydroxylase$ (CYP7A1), and low density lipoprotein receptor (LDLR) gene expression. Consistently, TEP and PWE showed similar hypolipidemic capacity in vivo, but significantly enhanced hypocholesterolemic capacity in terms of SREBP2, HMGCR, and bile salt export pump (BSEP) gene expression. Conclusion: These results suggest that column extraction after hot water extraction may be a good strategy to enhance tannins and long-chain fatty acid amides, which might cause stimulation of hypocholesterolemic actions through downregulation of cholesterol biosynthesis gene expression and upregulation of LDL receptor gene expression.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.16 no.3
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• pp.130-137
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• 2023

Smart farms, which have been receiving attention as a solution to recent rural problems, refer to technologies that optimize the growing environment of crops and increase the productivity and quality of crops through efficient management. If the relationships between environmental data in smart farms are analyzed, additional productivity enhancement and crop management will be possible. In this paper, we propose a method for acquiring and analyzing nine environmental data, including temperature, humidity, CO2, soil temperature, soil moisture, insolation, soil EC, EC, and pH. Data acquisition is done through RS-485 communication between the main board and the sensor board and stored in the database after acquisition. The stored data is downloaded in Excel sheet format and analyzed through histograms, data charts, and correlation heatmaps. First, we analyze the distribution of total, day, and night data through histogram analysis, and identifiy the average, median, minimum, and maximum values by month through data chart analysis separating day and night to see how the data changes by month. Finally, we analyze the correlation of the data through a correlation heatmap analysis separating day and night. The results show a very strong positive correlation between temperature and soil temperature and soil EC and EC during the day, and a very strong positive correlation between temperature and soil temperature and soil EC and EC at night, and a strong negative correlation between temperature and soil EC.

• Science of Emotion and Sensibility
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• v.13 no.3
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• pp.533-540
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• 2010

This study was designed to examine the difference in visual attention between the evaluations of facial attractiveness and facial trustworthiness, both of which may be the two most fundamental social evaluation for forming first impressions under various types of social interactions. In study 1, participants were asked to evaluate the attractiveness and trustworthiness of 40 new faces while their gaze directions being recorded using an eye-tracker. The analysis revealed that participants spent significantly longer gaze fixation time while examining certain facial features such as eyes and nose during the evaluation of facial trustworthiness, as compared to facial attractiveness. In study 2, participants performed the same face evaluation tasks, except that a word was briefly displayed on a certain facial feature in each face trial, which were then followed by unexpected recall tests of the previously viewed words. The analysis demonstrated that the recognition rate of the words that had been presented on the nose was significantly higher for the task of facial trustworthiness vs. facial attractiveness evaluation. These findings suggest that the evaluation of facial trustworthiness may be distinguished by that of facial attractiveness in terms of the allocation of attentional resources.

• Journal of the Korea Society for Simulation
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• v.31 no.3
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• pp.55-61
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• 2022

Currently police and fire departments use a Network/Wifi/GPS based emergency location positioning system established by mobile carriers to directly link with the device of the people who request the rescue to accurately position the expected location in the call area. However in the case of mountain rescue it is difficult to rescue the victim in golden time because the location of the search area cannot be limited when the victim is located in a radio shadow area of the mountain or the device power is off and this situation become worse if victim fail to report 911 by himself due to the injury. In this paper, we are expected to solve the previous problem by propose the mobile telecommunication forensic simulator consist of time series of cell information, human mobility model which include some general and specific features (age, gender, behavioral characteristics of victim, etc.) and intelligent infer system. The results of analysis appear in heatmap of polygons on the map based on the probability of the expected location information of the victim. With this technology we are expected to contribute to rapid and accurate lifesaving by reducing the search area of rescue team.

• The Journal of Information Systems
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• v.31 no.3
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• pp.177-195
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• 2022

Purpose This paper aims to prepare a full operational readiness by establishing an optimal flight plan considering the weather conditions in order to effectively perform the mission and operation of military aircraft. This paper suggests a flight prediction model and rules by analyzing the correlation between flight implementation and cancellation according to weather conditions by using big data collected from historical flight information of military aircraft supplied by Korean manufacturers and meteorological information from the Korea Meteorological Administration. In addition, by deriving flight rules according to weather information, it was possible to discover an efficient flight schedule establishment method in consideration of weather information. Design/methodology/approach This study is an analytic study using data mining techniques based on flight historical data of 44,558 flights of military aircraft accumulated by the Republic of Korea Air Force for a total of 36 months from January 2013 to December 2015 and meteorological information provided by the Korea Meteorological Administration. Four steps were taken to develop optimal flight prediction models and to derive rules for flight implementation and cancellation. First, a total of 10 independent variables and one dependent variable were used to develop the optimal model for flight implementation according to weather condition. Second, optimal flight prediction models were derived using algorithms such as logistics regression, Adaboost, KNN, Random forest and LightGBM, which are data mining techniques. Third, we collected the opinions of military aircraft pilots who have more than 25 years experience and evaluated importance level about independent variables using Python heatmap to develop flight implementation and cancellation rules according to weather conditions. Finally, the decision tree model was constructed, and the flight rules were derived to see how the weather conditions at each airport affect the implementation and cancellation of the flight. Findings Based on historical flight information of military aircraft and weather information of flight zone. We developed flight prediction model using data mining techniques. As a result of optimal flight prediction model development for each airbase, it was confirmed that the LightGBM algorithm had the best prediction rate in terms of recall rate. Each flight rules were checked according to the weather condition, and it was confirmed that precipitation, humidity, and the total cloud had a significant effect on flight cancellation. Whereas, the effect of visibility was found to be relatively insignificant. When a flight schedule was established, the rules will provide some insight to decide flight training more systematically and effectively.

• Journal of Life Science
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• v.33 no.5
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• pp.397-405
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• 2023

Although glutathione (GSH) has been shown to play an important role in the prevention of oxidative damage as an antioxidant, studies on immune regulation by it have not been properly conducted. In this study, we investigated whether luthione^®, a reduced GSH, has an immune enhancing effect in murine macrophage RAW 264.7 cells. The results of flow cytometry and immunofluorescence experiments indicated that luthione increased phagocytic activity, a representative function of macrophages, compared to the control cells. According to the results of the cytokine array, the expression of interleukin (IL)-5, IL-1β, and IL-27 was significantly increased in the luthione-treated cells. Luthione also enhanced the production of tumor necrosis factor-α and IL-1β through increased expression of their proteins, and increased release of the immune mediators such as nitric oxide (NO) and prostaglandin E₂ was associated with increased expression of inducible NO synthase and cyclooxygenase-2. In addition, the expression of cluster of differentiation 86, an M1 macrophage marker, was dramatically enhanced in RAW 264.7 cells treated with luthione. Furthermore, as a result of heat map analysis, we found that cytokine signaling 1/3-mediated signal transducer and activator of transcription/Janus tyrosine kinase signaling pathway was involved in the immunomodulatory effect by luthione. In conclusion, our data suggested that luthione could act as a molecular regulator in M1 macrophage polarization and enhance immune capacity by promoting macrophage phagocytic function.